BBa_K273022
1
E1A3
E1A3
2009-10-16T11:00:00Z
2015-05-08T01:11:44Z
Desigend by the Uppsala iGEM Team 2009
__NOTOC__
<partinfo>BBa_K273022 short</partinfo>
E1A2 antisense RNA against the formation of pyruvate dehydrogenase complex (PDC). Target is pyruvate dehydrogenase E1
(EC 1.2.4.1), in partuclar the alfa subunit (A). Antisense RNA approach #3.<br>
Will be transcribed together with the BioBrick interface and may affect the performance of the antisense RNA molecule.<br>
EXYZ<br>
EX: E1 enzyme subunit of the PDC complex<br>
Y: a=Alpha subunit of the particular enzyme subunit<br>
b=beta subunit of the particular enzyme subunit<br>
Z: # of antisense RNA approach<br>
==Usage and Biology==
Antisense RNA function supposed to fuction through either Shine Dalgarno blocking and/or RNA elongation blocking.
false
false
_373_
0
5250
9
Not in stock
false
BioBrick interace will be transcribed as well and may influence the performance of the antisense molecule.
false
Karl Brune
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation4184
1
stem_loop
range4184
1
12
55
annotation7018
1
BBa_B0010
range7018
1
1
80
BBa_K273034
1
E1A3 - Ter
E1A3 - Ter
2009-10-17T11:00:00Z
2015-05-08T01:11:44Z
-
-
false
false
_373_
0
5250
9
It's complicated
false
-
false
Karl Brune
component2049768
1
BBa_B0010
component2049767
1
BBa_K273022
component2049770
1
BBa_B0012
annotation2049768
1
BBa_B0010
range2049768
1
59
138
annotation2049770
1
BBa_B0012
range2049770
1
147
187
annotation2049767
1
BBa_K273022
range2049767
1
1
50
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1690
1
polya
range1690
1
28
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1687
1
stop
range1687
1
34
34
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_K273034_sequence
1
tctgaaaccatagtacaatttgcagtagataaaggggaaggggttttatatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K273022_sequence
1
tctgaaaccatagtacaatttgcagtagataaaggggaaggggttttata
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z