LOCUS       BBa_K273056             3109 bp    DNA     linear       26-Jun-2024
DEFINITION  PLlac - RBS - pdc - RBS - adh2(Z) - Ter
ACCESSION   BBa_K273056
VERSION     BBa_K273056.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     misc_feature    82..84
                     /label=startcodon
     misc_feature    1786..1791
                     /label=stopcodon (double)
     CDS             82..1785
                     /label=coding sequence
     CDS             82..1791
                     /label=BBa_K273000
     misc_feature    1804..1807
                     /label=conserved
     RBS             1800..1811
                     /label=BBa_B0034
     misc_feature    1818..1820
                     /label=startcodon
     misc_feature    2967..2972
                     /label=stopcodon (double)
     CDS             1818..2966
                     /label=cds adh2
     CDS             1818..2972
                     /label=BBa_K273001
     stem_loop       2992..3035
                     /label=stem_loop
     misc_feature    2981..3060
                     /label=BBa_B0010
     terminator      2981..3060
                     /label=BBa_B0010
     stem_loop       3076..3095
                     /label=T7 TE
     misc_feature    3102..3102
                     /label=stop
     polyA_site      3096..3109
                     /label=polya
     misc_feature    3069..3109
                     /label=BBa_B0012
     terminator      3069..3109
                     /label=BBa_B0012
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatgagttat actgtcggta cctatttagc ggagcggctt
      121 gtccagattg gtctcaagca tcacttcgca gtcgcgggcg actacaacct cgtccttctt
      181 gacaacctgc ttttgaacaa aaacatggag caggtttatt gctgtaacga actgaactgc
      241 ggtttcagtg cagaaggtta tgctcgtgcc aaaggcgcag cagcagccgt cgttacctac
      301 agcgtcggtg cgctttccgc atttgatgct atcggtggcg cctatgcaga aaaccttccg
      361 gttatcctga tctccggtgc tccgaacaac aatgatcacg ctgctggtca cgtgttgcat
      421 cacgctcttg gcaaaaccga ctatcactat cagttggaaa tggccaagaa catcacggcc
      481 gccgctgaag cgatttacac cccggaagaa gctccggcta aaatcgatca cgtgattaaa
      541 actgctcttc gtgagaagaa gccggtttat ctcgaaatcg cttgcaacat tgcttccatg
      601 ccctgcgccg ctcctggacc ggcaagcgca ttgttcaatg acgaagccag cgacgaagct
      661 tctttgaatg cagcggttga agaaaccctg aaattcatcg ccaaccgcga caaagttgcc
      721 gtcctcgtcg gcagcaagct gcgcgcagct ggtgctgaag aagctgctgt caaatttgct
      781 gatgctctcg gtggcgcagt tgctaccatg gctgctgcaa aaagcttctt cccagaagaa
      841 aacccgcatt acatcggcac ctcatggggt gaagtcagct atccgggcgt tgaaaagacg
      901 atgaaagaag ccgatgcggt tatcgctctg gctcctgtct tcaacgacta ctccaccact
      961 ggttggacgg atattcctga tcctaagaaa ctggttctcg ctgaaccgcg ttctgtcgtc
     1021 gttaacggca ttcgcttccc cagcgtccat ctgaaagact atctgacccg tttggctcag
     1081 aaagtttcca agaaaaccgg tgcattggac ttcttcaaat ccctcaatgc aggtgaactg
     1141 aagaaagccg ctccggctga tccgagtgct ccgttggtca acgcagaaat cgcccgtcag
     1201 gtcgaagctc ttctgacccc gaacacgacg gttattgctg aaaccggtga ctcttggttc
     1261 aatgctcagc gcatgaagct cccgaacggt gctcgcgttg aatatgaaat gcagtggggt
     1321 cacattggtt ggtccgttcc tgccgccttc ggttatgccg tcggtgctcc ggaacgtcgc
     1381 aacatcctca tggttggtga tggttccttc cagctgacgg ctcaggaagt cgctcagatg
     1441 gttcgcctga aactgccggt tatcatcttc ttgatcaata actatggtta caccatcgaa
     1501 gttatgatcc atgatggtcc gtacaacaac atcaagaact gggattatgc cggtctgatg
     1561 gaagtgttca acggtaacgg tggttatgac agcggtgctg gtaaaggcct gaaggctaaa
     1621 accggtggcg aactggcaga agctatcaag gttgctctgg caaacaccga cggcccaacc
     1681 ctgatcgaat gcttcatcgg tcgtgaagac tgcactgaag aattggtcaa atggggtaag
     1741 cgcgttgctg ccgccaacag ccgtaagcct gttaacaagc tcctctaata atactagaga
     1801 aagaggagaa atactagatg gcttcttcaa ctttttatat tcctttcgtc aacgaaatgg
     1861 gcgaaggttc gcttgaaaaa gcaatcaagg atcttaacgg cagcggcttt aaaaatgcgc
     1921 tgatcgtttc tgatgctttc atgaacaaat ccggtgttgt gaagcaggtt gctgacctgt
     1981 tgaaagcaca gggtattaat tctgctgttt atgatggcgt tatgccgaac ccgactgtta
     2041 ccgcagttct ggaaggcctt aagatcctga aggataacaa ttcagacttc gtcatctccc
     2101 tcggtggtgg ttctccccat gactgcgcca aagccatcgc tctggtcgca accaatggtg
     2161 gtgaagtcaa agactacgaa ggtatcgaca aatctaagaa acctgccctg cctttgatgt
     2221 caatcaacac gacggctggt acggcttctg aaatgacgcg tttctgcatc atcactgatg
     2281 aagtccgtca cgttaagatg gccattgttg accgtcacgt taccccgatg gtttccgtca
     2341 acgatcctct gttgatggtt ggtatgccaa aaggcctgac cgccgccacc ggtatggatg
     2401 ctctgaccca cgcatttgaa gcttattctt caacggcagc tactccgatc accgatgctt
     2461 gcgctttgaa agcagcttcc atgatcgcta agaatctgaa gaccgcttgc gacaacggta
     2521 aggatatgcc ggctcgtgaa gctatggctt atgcccaatt cctcgctggt atggccttca
     2581 acaacgcttc gcttggttat gtccatgcta tggctcacca gttgggcggt tactacaacc
     2641 tgccgcatgg tgtctgcaac gctgttctgc ttccgcatgt tctggcttat aacgcctctg
     2701 tcgttgctgg tcgtctgaaa gacgttggtg ttgctatggg tctcgatatc gccaatctcg
     2761 gtgataaaga aggcgcagaa gccaccattc aggctgttcg cgatctggct gcttccattg
     2821 gtattccagc aaacctgacc gagctgggtg ctaagaaaga agatgtgccg cttcttgctg
     2881 accacgctct gaaagatgct tgtgctctga ccaacccgcg tcagggtgat cagaaagaag
     2941 ttgaagaact cttcctgagc gctttctaat aatactagag ccaggcatca aataaaacga
     3001 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     3061 tactagagtc acactggctc accttcgggt gggcctttct gcgtttata
//