##gff-version 3
##sequence-region BBa_K273056 1 3109
BBa_K273056	.	ribosome_entry_site	1800	1811	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K273056	.	conserved_region	1804	1807	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K273056	.	CDS	82	1791	.	+	0	ID=BBa_K273000;Name=BBa_K273000
BBa_K273056	.	CDS	82	1785	.	+	0	ID=annotation2018225;Name=coding sequence;Parent=BBa_K273000
BBa_K273056	.	start_codon	82	84	.	+	0	ID=annotation2018223;Name=startcodon;Parent=BBa_K273000
BBa_K273056	.	stop_codon	1786	1791	.	+	0	ID=annotation2018224;Name=stopcodon (double);Parent=BBa_K273000
BBa_K273056	.	ribosome_entry_site	64	75	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K273056	.	conserved_region	68	71	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K273056	.	terminator	2981	3060	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K273056	.	stem_loop	2992	3035	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K273056	.	engineered_region	2981	3060	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K273056	.	CDS	1818	2972	.	+	0	ID=BBa_K273001;Name=BBa_K273001
BBa_K273056	.	stop_codon	2967	2972	.	+	0	ID=annotation2018227;Name=stopcodon (double);Parent=BBa_K273001
BBa_K273056	.	CDS	1818	2966	.	+	0	ID=annotation2018228;Name=cds adh2;Parent=BBa_K273001
BBa_K273056	.	start_codon	1818	1820	.	+	0	ID=annotation2018226;Name=startcodon;Parent=BBa_K273001
BBa_K273056	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_K273056	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_K273056	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_K273056	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_K273056	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_K273056	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_K273056	.	terminator	3069	3109	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K273056	.	stem_loop	3076	3095	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K273056	.	stop_codon	3102	3102	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K273056	.	polyA_site	3096	3109	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K273056	.	engineered_region	3069	3109	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
>BBa_K273056
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatact
agatgagttatactgtcggtacctatttagcggagcggcttgtccagattggtctcaagcatcacttcgcagtcgcggg
cgactacaacctcgtccttcttgacaacctgcttttgaacaaaaacatggagcaggtttattgctgtaacgaactgaac
tgcggtttcagtgcagaaggttatgctcgtgccaaaggcgcagcagcagccgtcgttacctacagcgtcggtgcgcttt
ccgcatttgatgctatcggtggcgcctatgcagaaaaccttccggttatcctgatctccggtgctccgaacaacaatga
tcacgctgctggtcacgtgttgcatcacgctcttggcaaaaccgactatcactatcagttggaaatggccaagaacatc
acggccgccgctgaagcgatttacaccccggaagaagctccggctaaaatcgatcacgtgattaaaactgctcttcgtg
agaagaagccggtttatctcgaaatcgcttgcaacattgcttccatgccctgcgccgctcctggaccggcaagcgcatt
gttcaatgacgaagccagcgacgaagcttctttgaatgcagcggttgaagaaaccctgaaattcatcgccaaccgcgac
aaagttgccgtcctcgtcggcagcaagctgcgcgcagctggtgctgaagaagctgctgtcaaatttgctgatgctctcg
gtggcgcagttgctaccatggctgctgcaaaaagcttcttcccagaagaaaacccgcattacatcggcacctcatgggg
tgaagtcagctatccgggcgttgaaaagacgatgaaagaagccgatgcggttatcgctctggctcctgtcttcaacgac
tactccaccactggttggacggatattcctgatcctaagaaactggttctcgctgaaccgcgttctgtcgtcgttaacg
gcattcgcttccccagcgtccatctgaaagactatctgacccgtttggctcagaaagtttccaagaaaaccggtgcatt
ggacttcttcaaatccctcaatgcaggtgaactgaagaaagccgctccggctgatccgagtgctccgttggtcaacgca
gaaatcgcccgtcaggtcgaagctcttctgaccccgaacacgacggttattgctgaaaccggtgactcttggttcaatg
ctcagcgcatgaagctcccgaacggtgctcgcgttgaatatgaaatgcagtggggtcacattggttggtccgttcctgc
cgccttcggttatgccgtcggtgctccggaacgtcgcaacatcctcatggttggtgatggttccttccagctgacggct
caggaagtcgctcagatggttcgcctgaaactgccggttatcatcttcttgatcaataactatggttacaccatcgaag
ttatgatccatgatggtccgtacaacaacatcaagaactgggattatgccggtctgatggaagtgttcaacggtaacgg
tggttatgacagcggtgctggtaaaggcctgaaggctaaaaccggtggcgaactggcagaagctatcaaggttgctctg
gcaaacaccgacggcccaaccctgatcgaatgcttcatcggtcgtgaagactgcactgaagaattggtcaaatggggta
agcgcgttgctgccgccaacagccgtaagcctgttaacaagctcctctaataatactagagaaagaggagaaatactag
atggcttcttcaactttttatattcctttcgtcaacgaaatgggcgaaggttcgcttgaaaaagcaatcaaggatctta
acggcagcggctttaaaaatgcgctgatcgtttctgatgctttcatgaacaaatccggtgttgtgaagcaggttgctga
cctgttgaaagcacagggtattaattctgctgtttatgatggcgttatgccgaacccgactgttaccgcagttctggaa
ggccttaagatcctgaaggataacaattcagacttcgtcatctccctcggtggtggttctccccatgactgcgccaaag
ccatcgctctggtcgcaaccaatggtggtgaagtcaaagactacgaaggtatcgacaaatctaagaaacctgccctgcc
tttgatgtcaatcaacacgacggctggtacggcttctgaaatgacgcgtttctgcatcatcactgatgaagtccgtcac
gttaagatggccattgttgaccgtcacgttaccccgatggtttccgtcaacgatcctctgttgatggttggtatgccaa
aaggcctgaccgccgccaccggtatggatgctctgacccacgcatttgaagcttattcttcaacggcagctactccgat
caccgatgcttgcgctttgaaagcagcttccatgatcgctaagaatctgaagaccgcttgcgacaacggtaaggatatg
ccggctcgtgaagctatggcttatgcccaattcctcgctggtatggccttcaacaacgcttcgcttggttatgtccatg
ctatggctcaccagttgggcggttactacaacctgccgcatggtgtctgcaacgctgttctgcttccgcatgttctggc
ttataacgcctctgtcgttgctggtcgtctgaaagacgttggtgttgctatgggtctcgatatcgccaatctcggtgat
aaagaaggcgcagaagccaccattcaggctgttcgcgatctggctgcttccattggtattccagcaaacctgaccgagc
tgggtgctaagaaagaagatgtgccgcttcttgctgaccacgctctgaaagatgcttgtgctctgaccaacccgcgtca
gggtgatcagaaagaagttgaagaactcttcctgagcgctttctaataatactagagccaggcatcaaataaaacgaaa
ggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctca
ccttcgggtgggcctttctgcgtttata