BBa_K273056
type: ComponentDefinition
persistent identity: BBa K273056
display id: BBa_K273056
version: 1
was generated by: iGEM to SBOL conversion
description: PLlac - RBS - pdc - RBS - adh2(Z) - Ter
created: 2009-10-17T11:00:00Z
modified: 2015-05-08T01:11:45Z
mutable provenance: -
owned by: james
owned by: myers
mutable description: -
discontinued: false
dominant: false
group u list: _373_
m user id: 0
owner id: 5250
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: -
star: false
creator: Karl Brune
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_B0010
component: BBa_K273001
component: BBa_B0012
component: BBa_B0034
component: BBa_R0011
component: BBa_K273000
sequence annotation: BBa_R0011
sequence annotation: BBa_B0034
sequence annotation: BBa_K273000
sequence annotation: BBa_K273001
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K273056_sequence/1

BBa_B0010
type: Component
persistent identity: component2051125
display id: component2051125
version: 1
top level: BBa_K273056
definition: BBa_B0010
access: public

BBa_K273001
type: Component
persistent identity: component2051124
display id: component2051124
version: 1
top level: BBa_K273056
definition: adh2
access: public

BBa_B0012
type: Component
persistent identity: component2051127
display id: component2051127
version: 1
top level: BBa_K273056
definition: BBa_B0012
access: public

BBa_B0034
type: Component
persistent identity: component2051114
display id: component2051114
version: 1
top level: BBa_K273056
definition: BBa_B0034
access: public

BBa_R0011
type: Component
persistent identity: component2051108
display id: component2051108
version: 1
top level: BBa_K273056
definition: lacI+pL
access: public

BBa_B0034
type: Component
persistent identity: component2051120
display id: component2051120
version: 1
top level: BBa_K273056
definition: BBa_B0034
access: public

BBa_K273000
type: Component
persistent identity: component2051118
display id: component2051118
version: 1
top level: BBa_K273056
definition: pdc
access: public

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2051108
display id: annotation2051108
version: 1
top level: BBa_K273056
location: https://synbiohub.org/public/igem/BBa_K273056/annotation2051108/range2051108/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_K273056/annotation2051108/range2051108/1
type: Range
persistent identity: range2051108
display id: range2051108
version: 1
top level: BBa_K273056
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2051114
display id: annotation2051114
version: 1
top level: BBa_K273056
location: https://synbiohub.org/public/igem/BBa_K273056/annotation2051114/range2051114/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K273056/annotation2051114/range2051114/1
type: Range
persistent identity: range2051114
display id: range2051114
version: 1
top level: BBa_K273056
direction: direction/reverse
start: 64
end: 75
orientation: inline

BBa_K273000
type: SequenceAnnotation
persistent identity: annotation2051118
display id: annotation2051118
version: 1
top level: BBa_K273056
location: https://synbiohub.org/public/igem/BBa_K273056/annotation2051118/range2051118/1
component: BBa_K273000

https://synbiohub.org/public/igem/BBa_K273056/annotation2051118/range2051118/1
type: Range
persistent identity: range2051118
display id: range2051118
version: 1
top level: BBa_K273056
direction: direction/reverse
start: 82
end: 1791
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2051120
display id: annotation2051120
version: 1
top level: BBa_K273056
location: https://synbiohub.org/public/igem/BBa_K273056/annotation2051120/range2051120/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K273056/annotation2051120/range2051120/1
type: Range
persistent identity: range2051120
display id: range2051120
version: 1
top level: BBa_K273056
direction: direction/reverse
start: 1800
end: 1811
orientation: inline

BBa_K273001
type: SequenceAnnotation
persistent identity: annotation2051124
display id: annotation2051124
version: 1
top level: BBa_K273056
location: https://synbiohub.org/public/igem/BBa_K273056/annotation2051124/range2051124/1
component: BBa_K273001

https://synbiohub.org/public/igem/BBa_K273056/annotation2051124/range2051124/1
type: Range
persistent identity: range2051124
display id: range2051124
version: 1
top level: BBa_K273056
direction: direction/reverse
start: 1818
end: 2972
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2051125
display id: annotation2051125
version: 1
top level: BBa_K273056
location: https://synbiohub.org/public/igem/BBa_K273056/annotation2051125/range2051125/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K273056/annotation2051125/range2051125/1
type: Range
persistent identity: range2051125
display id: range2051125
version: 1
top level: BBa_K273056
direction: direction/forward
start: 2981
end: 3060
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2051127
display id: annotation2051127
version: 1
top level: BBa_K273056
location: https://synbiohub.org/public/igem/BBa_K273056/annotation2051127/range2051127/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K273056/annotation2051127/range2051127/1
type: Range
persistent identity: range2051127
display id: range2051127
version: 1
top level: BBa_K273056
direction: direction/forward
start: 3069
end: 3109
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

lacI+pL
type: ComponentDefinition
persistent identity: BBa R0011
display id: BBa_R0011
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (lacI regulated, lambda pL hybrid)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: represillator of Elowitz and Leibler (2000)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference).
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site. LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs.
star: true
creator: Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0011
sequence annotation: lac O1
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0011_sequence/1

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation7064
display id: annotation7064
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
type: Range
persistent identity: range7064
display id: range7064
version: 1
top level: lacI+pL
direction: direction/forward
start: 1
end: 54
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation1999
display id: annotation1999
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
type: Range
persistent identity: range1999
display id: range1999
version: 1
top level: lacI+pL
direction: direction/forward
start: 3
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2000
display id: annotation2000
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
type: Range
persistent identity: range2000
display id: range2000
version: 1
top level: lacI+pL
direction: direction/forward
start: 20
end: 25
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation2001
display id: annotation2001
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
type: Range
persistent identity: range2001
display id: range2001
version: 1
top level: lacI+pL
direction: direction/forward
start: 26
end: 42
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2002
display id: annotation2002
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
type: Range
persistent identity: range2002
display id: range2002
version: 1
top level: lacI+pL
direction: direction/forward
start: 43
end: 48
orientation: inline

pdc
type: ComponentDefinition
persistent identity: BBa K273000
display id: BBa_K273000
version: 1
was generated by: iGEM to SBOL conversion
description: Pyruvate decarboxylase (pdc) from Z. Mobilis ATCC 29191
created: 2009-08-31T11:00:00Z
modified: 2015-05-08T01:11:44Z
mutable provenance: Source of the Pyruvate decarboxylase (pdc) is Z. Mobilis ATCC 29191 via Phusion PCR with the following primers: pdc_forward CGCgaattcgcggccgcttctagATGAGTTATACTGTCGGTACC 44nt pdc_reverse CGctgcagcggccgctactagtaTTATTAGAGGAGCTTGTTAACAGGC 48nt
owned by: james
owned by: myers
mutable description: The pdc converts pyruvate to acetaldehyde. Acetaldehyde can be then furhter converted to ethanol via the adh2 (alcohol dehydrogenase).
discontinued: false
dominant: false
experience: experience/None
group u list: _373_
m user id: 0
owner id: 5250
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: none
star: false
creator: Karl Brune
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: coding sequence
sequence annotation: startcodon
sequence annotation: stopcodon (double)
sequence: https://synbiohub.org/public/igem/BBa_K273000_sequence/1

coding sequence
type: SequenceAnnotation
persistent identity: annotation2018225
display id: annotation2018225
version: 1
top level: pdc
location: https://synbiohub.org/public/igem/BBa_K273000/annotation2018225/range2018225/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K273000/annotation2018225/range2018225/1
type: Range
persistent identity: range2018225
display id: range2018225
version: 1
top level: pdc
direction: direction/forward
start: 1
end: 1704
orientation: inline

startcodon
type: SequenceAnnotation
persistent identity: annotation2018223
display id: annotation2018223
version: 1
top level: pdc
location: https://synbiohub.org/public/igem/BBa_K273000/annotation2018223/range2018223/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K273000/annotation2018223/range2018223/1
type: Range
persistent identity: range2018223
display id: range2018223
version: 1
top level: pdc
direction: direction/forward
start: 1
end: 3
orientation: inline

stopcodon (double)
type: SequenceAnnotation
persistent identity: annotation2018224
display id: annotation2018224
version: 1
top level: pdc
location: https://synbiohub.org/public/igem/BBa_K273000/annotation2018224/range2018224/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K273000/annotation2018224/range2018224/1
type: Range
persistent identity: range2018224
display id: range2018224
version: 1
top level: pdc
direction: direction/forward
start: 1705
end: 1710
orientation: inline

adh2
type: ComponentDefinition
persistent identity: BBa K273001
display id: BBa_K273001
version: 1
was generated by: iGEM to SBOL conversion
description: Alcohol dehydrogenase (adh2 , adhII, adhB) from Z. Mobilis ATCC 29191
created: 2009-08-31T11:00:00Z
modified: 2015-05-08T01:11:44Z
mutable provenance: Zymomonas mobilis, Z. Mobilis, ATCC 29191
owned by: james
owned by: myers
mutable description: Acetaldehyde is converted to ethanol via the adh2 (alcohol dehydrogenase). Acetalydehyde can be produced by the conversion of pyruvate to acetaldehyde by the pyruvate decarboxylase (pdc) with this part <partinfo>BBa_K273000</partinfo>
discontinued: false
dominant: false
experience: experience/None
group u list: _373_
m user id: 0
owner id: 5250
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: none
star: false
creator: Karl Brune
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: startcodon
sequence annotation: cds adh2
sequence annotation: stopcodon (double)
sequence: https://synbiohub.org/public/igem/BBa_K273001_sequence/1

startcodon
type: SequenceAnnotation
persistent identity: annotation2018226
display id: annotation2018226
version: 1
top level: adh2
location: https://synbiohub.org/public/igem/BBa_K273001/annotation2018226/range2018226/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K273001/annotation2018226/range2018226/1
type: Range
persistent identity: range2018226
display id: range2018226
version: 1
top level: adh2
direction: direction/forward
start: 1
end: 3
orientation: inline

cds adh2
type: SequenceAnnotation
persistent identity: annotation2018228
display id: annotation2018228
version: 1
top level: adh2
location: https://synbiohub.org/public/igem/BBa_K273001/annotation2018228/range2018228/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K273001/annotation2018228/range2018228/1
type: Range
persistent identity: range2018228
display id: range2018228
version: 1
top level: adh2
direction: direction/forward
start: 1
end: 1149
orientation: inline

stopcodon (double)
type: SequenceAnnotation
persistent identity: annotation2018227
display id: annotation2018227
version: 1
top level: adh2
location: https://synbiohub.org/public/igem/BBa_K273001/annotation2018227/range2018227/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K273001/annotation2018227/range2018227/1
type: Range
persistent identity: range2018227
display id: range2018227
version: 1
top level: adh2
direction: direction/forward
start: 1150
end: 1155
orientation: inline

https://synbiohub.org/public/igem/BBa_K273056_sequence/1
type: Sequence
persistent identity: BBa K273056 sequence
display id: BBa_K273056_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatgagttatactgtcggtacctatttagcggagcggcttgtccagattggtctcaagcatcacttcgcagtcgcgggcgactacaacctcgtccttcttgacaacctgcttttgaacaaaaacatggagcaggtttattgctgtaacgaactgaactgcggtttcagtgcagaaggttatgctcgtgccaaaggcgcagcagcagccgtcgttacctacagcgtcggtgcgctttccgcatttgatgctatcggtggcgcctatgcagaaaaccttccggttatcctgatctccggtgctccgaacaacaatgatcacgctgctggtcacgtgttgcatcacgctcttggcaaaaccgactatcactatcagttggaaatggccaagaacatcacggccgccgctgaagcgatttacaccccggaagaagctccggctaaaatcgatcacgtgattaaaactgctcttcgtgagaagaagccggtttatctcgaaatcgcttgcaacattgcttccatgccctgcgccgctcctggaccggcaagcgcattgttcaatgacgaagccagcgacgaagcttctttgaatgcagcggttgaagaaaccctgaaattcatcgccaaccgcgacaaagttgccgtcctcgtcggcagcaagctgcgcgcagctggtgctgaagaagctgctgtcaaatttgctgatgctctcggtggcgcagttgctaccatggctgctgcaaaaagcttcttcccagaagaaaacccgcattacatcggcacctcatggggtgaagtcagctatccgggcgttgaaaagacgatgaaagaagccgatgcggttatcgctctggctcctgtcttcaacgactactccaccactggttggacggatattcctgatcctaagaaactggttctcgctgaaccgcgttctgtcgtcgttaacggcattcgcttccccagcgtccatctgaaagactatctgacccgtttggctcagaaagtttccaagaaaaccggtgcattggacttcttcaaatccctcaatgcaggtgaactgaagaaagccgctccggctgatccgagtgctccgttggtcaacgcagaaatcgcccgtcaggtcgaagctcttctgaccccgaacacgacggttattgctgaaaccggtgactcttggttcaatgctcagcgcatgaagctcccgaacggtgctcgcgttgaatatgaaatgcagtggggtcacattggttggtccgttcctgccgccttcggttatgccgtcggtgctccggaacgtcgcaacatcctcatggttggtgatggttccttccagctgacggctcaggaagtcgctcagatggttcgcctgaaactgccggttatcatcttcttgatcaataactatggttacaccatcgaagttatgatccatgatggtccgtacaacaacatcaagaactgggattatgccggtctgatggaagtgttcaacggtaacggtggttatgacagcggtgctggtaaaggcctgaaggctaaaaccggtggcgaactggcagaagctatcaaggttgctctggcaaacaccgacggcccaaccctgatcgaatgcttcatcggtcgtgaagactgcactgaagaattggtcaaatggggtaagcgcgttgctgccgccaacagccgtaagcctgttaacaagctcctctaataatactagagaaagaggagaaatactagatggcttcttcaactttttatattcctttcgtcaacgaaatgggcgaaggttcgcttgaaaaagcaatcaaggatcttaacggcagcggctttaaaaatgcgctgatcgtttctgatgctttcatgaacaaatccggtgttgtgaagcaggttgctgacctgttgaaagcacagggtattaat…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0011_sequence/1
type: Sequence
persistent identity: BBa R0011 sequence
display id: BBa_R0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K273000_sequence/1
type: Sequence
persistent identity: BBa K273000 sequence
display id: BBa_K273000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagttatactgtcggtacctatttagcggagcggcttgtccagattggtctcaagcatcacttcgcagtcgcgggcgactacaacctcgtccttcttgacaacctgcttttgaacaaaaacatggagcaggtttattgctgtaacgaactgaactgcggtttcagtgcagaaggttatgctcgtgccaaaggcgcagcagcagccgtcgttacctacagcgtcggtgcgctttccgcatttgatgctatcggtggcgcctatgcagaaaaccttccggttatcctgatctccggtgctccgaacaacaatgatcacgctgctggtcacgtgttgcatcacgctcttggcaaaaccgactatcactatcagttggaaatggccaagaacatcacggccgccgctgaagcgatttacaccccggaagaagctccggctaaaatcgatcacgtgattaaaactgctcttcgtgagaagaagccggtttatctcgaaatcgcttgcaacattgcttccatgccctgcgccgctcctggaccggcaagcgcattgttcaatgacgaagccagcgacgaagcttctttgaatgcagcggttgaagaaaccctgaaattcatcgccaaccgcgacaaagttgccgtcctcgtcggcagcaagctgcgcgcagctggtgctgaagaagctgctgtcaaatttgctgatgctctcggtggcgcagttgctaccatggctgctgcaaaaagcttcttcccagaagaaaacccgcattacatcggcacctcatggggtgaagtcagctatccgggcgttgaaaagacgatgaaagaagccgatgcggttatcgctctggctcctgtcttcaacgactactccaccactggttggacggatattcctgatcctaagaaactggttctcgctgaaccgcgttctgtcgtcgttaacggcattcgcttccccagcgtccatctgaaagactatctgacccgtttggctcagaaagtttccaagaaaaccggtgcattggacttcttcaaatccctcaatgcaggtgaactgaagaaagccgctccggctgatccgagtgctccgttggtcaacgcagaaatcgcccgtcaggtcgaagctcttctgaccccgaacacgacggttattgctgaaaccggtgactcttggttcaatgctcagcgcatgaagctcccgaacggtgctcgcgttgaatatgaaatgcagtggggtcacattggttggtccgttcctgccgccttcggttatgccgtcggtgctccggaacgtcgcaacatcctcatggttggtgatggttccttccagctgacggctcaggaagtcgctcagatggttcgcctgaaactgccggttatcatcttcttgatcaataactatggttacaccatcgaagttatgatccatgatggtccgtacaacaacatcaagaactgggattatgccggtctgatggaagtgttcaacggtaacggtggttatgacagcggtgctggtaaaggcctgaaggctaaaaccggtggcgaactggcagaagctatcaaggttgctctggcaaacaccgacggcccaaccctgatcgaatgcttcatcggtcgtgaagactgcactgaagaattggtcaaatggggtaagcgcgttgctgccgccaacagccgtaagcctgttaacaagctcctctaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K273001_sequence/1
type: Sequence
persistent identity: BBa K273001 sequence
display id: BBa_K273001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcttcttcaactttttatattcctttcgtcaacgaaatgggcgaaggttcgcttgaaaaagcaatcaaggatcttaacggcagcggctttaaaaatgcgctgatcgtttctgatgctttcatgaacaaatccggtgttgtgaagcaggttgctgacctgttgaaagcacagggtattaattctgctgtttatgatggcgttatgccgaacccgactgttaccgcagttctggaaggccttaagatcctgaaggataacaattcagacttcgtcatctccctcggtggtggttctccccatgactgcgccaaagccatcgctctggtcgcaaccaatggtggtgaagtcaaagactacgaaggtatcgacaaatctaagaaacctgccctgcctttgatgtcaatcaacacgacggctggtacggcttctgaaatgacgcgtttctgcatcatcactgatgaagtccgtcacgttaagatggccattgttgaccgtcacgttaccccgatggtttccgtcaacgatcctctgttgatggttggtatgccaaaaggcctgaccgccgccaccggtatggatgctctgacccacgcatttgaagcttattcttcaacggcagctactccgatcaccgatgcttgcgctttgaaagcagcttccatgatcgctaagaatctgaagaccgcttgcgacaacggtaaggatatgccggctcgtgaagctatggcttatgcccaattcctcgctggtatggccttcaacaacgcttcgcttggttatgtccatgctatggctcaccagttgggcggttactacaacctgccgcatggtgtctgcaacgctgttctgcttccgcatgttctggcttataacgcctctgtcgttgctggtcgtctgaaagacgttggtgttgctatgggtctcgatatcgccaatctcggtgataaagaaggcgcagaagccaccattcaggctgttcgcgatctggctgcttccattggtattccagcaaacctgaccgagctgggtgctaagaaagaagatgtgccgcttcttgctgaccacgctctgaaagatgcttgtgctctgaccaacccgcgtcagggtgatcagaaagaagttgaagaactcttcctgagcgctttctaataa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z