LOCUS BBa_K273057 3483 bp DNA linear 27-Dec-2024 DEFINITION pPetE - RBS - pdc - RBS - adh2(Z) - Ter ACCESSION BBa_K273057 VERSION BBa_K273057.1 FEATURES Location/Qualifiers promoter 1..429 /label=BBa_K273019 misc_feature 442..445 /label=conserved RBS 438..449 /label=BBa_B0034 misc_feature 456..458 /label=startcodon misc_feature 2160..2165 /label=stopcodon (double) CDS 456..2159 /label=coding sequence CDS 456..2165 /label=BBa_K273000 misc_feature 2178..2181 /label=conserved RBS 2174..2185 /label=BBa_B0034 misc_feature 2192..2194 /label=startcodon misc_feature 3341..3346 /label=stopcodon (double) CDS 2192..3340 /label=cds adh2 CDS 2192..3346 /label=BBa_K273001 stem_loop 3366..3409 /label=stem_loop misc_feature 3355..3434 /label=BBa_B0010 terminator 3355..3434 /label=BBa_B0010 stem_loop 3450..3469 /label=T7 TE misc_feature 3476..3476 /label=stop polyA_site 3470..3483 /label=polya misc_feature 3443..3483 /label=BBa_B0012 terminator 3443..3483 /label=BBa_B0012 ORIGIN 1 tcatagcggt tgcccaatct aactcaggga gcgacttcag cccacaaaaa acaccactgg 61 gcctactggg ctattcccat tatcatctac attgaaggga tagcaagcta atttttatga 121 cggcgatcgc caaaaacaaa gaaaattcag caattaccgt gggtagcaaa aaatccccat 181 ctaaagttca gtaaatatag ctagaacaac caagcatttt cggcaaagta ctattcagat 241 agaacgagaa atgagcttgt tctatccgcc cggggctgag gctgtataat ctacgacggg 301 ctgtcaaaca ttgtgatacc atgggcagaa gaaaggaaaa acgtccctga tcgccttttt 361 gggcacggag tagggcgtta ccccggcccg ttcaaccaca agtccctata gatacaatcg 421 ccaagaagtt actagagaaa gaggagaaat actagatgag ttatactgtc ggtacctatt 481 tagcggagcg gcttgtccag attggtctca agcatcactt cgcagtcgcg ggcgactaca 541 acctcgtcct tcttgacaac ctgcttttga acaaaaacat ggagcaggtt tattgctgta 601 acgaactgaa ctgcggtttc agtgcagaag gttatgctcg tgccaaaggc gcagcagcag 661 ccgtcgttac ctacagcgtc ggtgcgcttt ccgcatttga tgctatcggt ggcgcctatg 721 cagaaaacct tccggttatc ctgatctccg gtgctccgaa caacaatgat cacgctgctg 781 gtcacgtgtt gcatcacgct cttggcaaaa ccgactatca ctatcagttg gaaatggcca 841 agaacatcac ggccgccgct gaagcgattt acaccccgga agaagctccg gctaaaatcg 901 atcacgtgat taaaactgct cttcgtgaga agaagccggt ttatctcgaa atcgcttgca 961 acattgcttc catgccctgc gccgctcctg gaccggcaag cgcattgttc aatgacgaag 1021 ccagcgacga agcttctttg aatgcagcgg ttgaagaaac cctgaaattc atcgccaacc 1081 gcgacaaagt tgccgtcctc gtcggcagca agctgcgcgc agctggtgct gaagaagctg 1141 ctgtcaaatt tgctgatgct ctcggtggcg cagttgctac catggctgct gcaaaaagct 1201 tcttcccaga agaaaacccg cattacatcg gcacctcatg gggtgaagtc agctatccgg 1261 gcgttgaaaa gacgatgaaa gaagccgatg cggttatcgc tctggctcct gtcttcaacg 1321 actactccac cactggttgg acggatattc ctgatcctaa gaaactggtt ctcgctgaac 1381 cgcgttctgt cgtcgttaac ggcattcgct tccccagcgt ccatctgaaa gactatctga 1441 cccgtttggc tcagaaagtt tccaagaaaa ccggtgcatt ggacttcttc aaatccctca 1501 atgcaggtga actgaagaaa gccgctccgg ctgatccgag tgctccgttg gtcaacgcag 1561 aaatcgcccg tcaggtcgaa gctcttctga ccccgaacac gacggttatt gctgaaaccg 1621 gtgactcttg gttcaatgct cagcgcatga agctcccgaa cggtgctcgc gttgaatatg 1681 aaatgcagtg gggtcacatt ggttggtccg ttcctgccgc cttcggttat gccgtcggtg 1741 ctccggaacg tcgcaacatc ctcatggttg gtgatggttc cttccagctg acggctcagg 1801 aagtcgctca gatggttcgc ctgaaactgc cggttatcat cttcttgatc aataactatg 1861 gttacaccat cgaagttatg atccatgatg gtccgtacaa caacatcaag aactgggatt 1921 atgccggtct gatggaagtg ttcaacggta acggtggtta tgacagcggt gctggtaaag 1981 gcctgaaggc taaaaccggt ggcgaactgg cagaagctat caaggttgct ctggcaaaca 2041 ccgacggccc aaccctgatc gaatgcttca tcggtcgtga agactgcact gaagaattgg 2101 tcaaatgggg taagcgcgtt gctgccgcca acagccgtaa gcctgttaac aagctcctct 2161 aataatacta gagaaagagg agaaatacta gatggcttct tcaacttttt atattccttt 2221 cgtcaacgaa atgggcgaag gttcgcttga aaaagcaatc aaggatctta acggcagcgg 2281 ctttaaaaat gcgctgatcg tttctgatgc tttcatgaac aaatccggtg ttgtgaagca 2341 ggttgctgac ctgttgaaag cacagggtat taattctgct gtttatgatg gcgttatgcc 2401 gaacccgact gttaccgcag ttctggaagg ccttaagatc ctgaaggata acaattcaga 2461 cttcgtcatc tccctcggtg gtggttctcc ccatgactgc gccaaagcca tcgctctggt 2521 cgcaaccaat ggtggtgaag tcaaagacta cgaaggtatc gacaaatcta agaaacctgc 2581 cctgcctttg atgtcaatca acacgacggc tggtacggct tctgaaatga cgcgtttctg 2641 catcatcact gatgaagtcc gtcacgttaa gatggccatt gttgaccgtc acgttacccc 2701 gatggtttcc gtcaacgatc ctctgttgat ggttggtatg ccaaaaggcc tgaccgccgc 2761 caccggtatg gatgctctga cccacgcatt tgaagcttat tcttcaacgg cagctactcc 2821 gatcaccgat gcttgcgctt tgaaagcagc ttccatgatc gctaagaatc tgaagaccgc 2881 ttgcgacaac ggtaaggata tgccggctcg tgaagctatg gcttatgccc aattcctcgc 2941 tggtatggcc ttcaacaacg cttcgcttgg ttatgtccat gctatggctc accagttggg 3001 cggttactac aacctgccgc atggtgtctg caacgctgtt ctgcttccgc atgttctggc 3061 ttataacgcc tctgtcgttg ctggtcgtct gaaagacgtt ggtgttgcta tgggtctcga 3121 tatcgccaat ctcggtgata aagaaggcgc agaagccacc attcaggctg ttcgcgatct 3181 ggctgcttcc attggtattc cagcaaacct gaccgagctg ggtgctaaga aagaagatgt 3241 gccgcttctt gctgaccacg ctctgaaaga tgcttgtgct ctgaccaacc cgcgtcaggg 3301 tgatcagaaa gaagttgaag aactcttcct gagcgctttc taataatact agagccaggc 3361 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt 3421 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt 3481 ata //