BBa_K274001 1 MelA melanin pigment 2009-08-02T11:00:00Z 2015-05-08T01:11:45Z The melA gene comes from the Rhizobium etli CFN 42 symbiotic plasmid p42d (accession number U80928). The origonal plasmid was created by Christine Nicole S. Santos and Gregory Stephanopoulos (reference: http://aem.asm.org/cgi/content/abstract/74/4/1190) Produces dark brown pigment output. The gene (melA) codes for a tyrosinase which produces the pigment from L-tyrosine. Production of the pigment requires the addition of copper and L-tyrosine supplements (the copper acts as a cofactor for the gene product) but no other precursors. The BioBrick sequence includes the native ribosome binding site. false false _374_ 0 5412 9 Not in stock false The only changes to the sequence from the plasmid kindly provided by The Massachusetts Institute of Technology and Gregory Stephanopoulos was the removal of two resitrction sites, and the addition of the iGEM prefix and suffix. The two PstI restriction sites were removed by converting CAG to CAA in the second codon of the restriction site. false Shuna Gould annotation2014738 1 MelA range2014738 1 15 1844 annotation2014737 1 rbs range2014737 1 1 8 annotation2014757 1 stop range2014757 1 1841 1844 annotation2014756 1 start range2014756 1 15 17 BBa_K274001_sequence 1 aggaggaataaaccatggcgtggctggtcggcaagccgtcgctcgaacgatcatggaatgcgatactaagttttccggaatctggattccaactggaatgcaggaatacgatcggtagtagcgtttttagctcgcattttacgctgcattttcgagtggcacgtcgcttgcttcatttctcgtgccgccgattcaccgaaactcagaaagagccaacgcaagctctatggtggtgcgaattgcccactgctcccgcgcccagacggcgcggaaccggcctaaaagcggcattaatcttggcgaaggacaattcaaatccaagggagagtaagatgagcatcacacgcagacatgtcatcgttcagggtggcgtcattgcagcaggcctgctcgccagcggcctaccggggacaaaagccttcgcgcagataccgtcaatcccttggcggcgctcactgcaaggcttggcctggaacgacccgatcatcgagacctatcgcgacgcagtgcgccttctcaacgcccttcccgccagcgacaaattcaactgggtcaacctctcgaaaattcacggcagcggtgacgtcgtcaaatactgcccgcatggcaactggtatttcctgccgtggcacagggcctatacggctatgtacgagcgcatcgttcggcacgtgaccaagaacaacgatttcgctatgccgttctgggactggaccgacaatccgtacctgcccgaagtgttcacaatgcaaaagacgcccgacggcaaggacaatccactttatgtttcgtcgcgcacctggccaatcacgcagccgatgccggacaatatagttgggccacaggttctcaacaccatcctaacggcgaagccatacgaggtcttcggcaccacccgccccgagggacagaactcactcgatccttcctgggtcaccaccagcagcggcacgcagggggcgctggaatacacaccgcacaatcaggtgcacaacaatatcggtggctggatgccggaaatgtcgtcgccccgcgatccgatcttcttcatgcatcattgcaacatcgaccgcatctgggcgacctggaatttgcgcaacgccaacagcacggatcgactctgggccgacatgccgttcaccgacaatttctacgatgtcgacggcaacttctggtccccgaaggtctctgacctttatgttccagaggaactcggatacaattatggtttccggacctacttcaaggtcgcggcggcgagcgccaaaacgctggccctgaacgataaactcacgtccgtgatcgcggcgacggcgaccgatgctgcaatcgccggcgtgacgaccacctccacggacaacagcaaggcggcaacggaaaacgtgccgctttcgctgccgatcaagatcccggcgggcgcattgcaggagatcgtccgccaaccgcctctgccatccggcatggatacaatggatttcggcgcggcacaggagcaggcggcctccgctcctcgtgtgctggcattcctgcgcgatgtcgagatcaccagtgccagcacgaccagcgttcgggtgtttctcggcaagaacgaccttaaggccgatacgcccgtcaccgatccccattacgtcggttctttcgccgttctcggtcatgacggcgaccatcatcgcaaaccatccttcgtcctcgatctgacggacgcgatccagcgggtttacggcggaagggggcagacggatggcgaggccatcgacctgcaactcattcctgtcggatcgggagcgggcaaacccggcgccgtagagcccgcaaagctagaaatagccatagtgtccgcctaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z