BBa_K274002 1 BBa_K274002 Vio operon 2009-10-07T11:00:00Z 2015-05-08T01:11:45Z The VioA-E genes were originally from from Chromobacterium voilaceum ATCC 12472 in the pPSX vio+ plasmid. This was kindly provided by John Pemberton; Department of Microbiology and parasitology, University of Queensland, Brisbane, Australia. (Sarovich & Pemberton (2007) Plasmid 57:306-313). The codons were optimised for E. coli by DNA2.0 (Welch, Villalobos, Gustafsson and Minshull (2009) J. R. Soc. Interface) Produces a purple pigment (violacein) from L-tyrosine. The operon contains five genes (VioA-E) each with their own ribosome binding sites. false false _374_ 0 5412 9 It's complicated true We added complementary restriction sites on either side of vioC and vioD (BamHI, BglII, HindIII). This allows them to be seperately removed to produce more colours from the operon. false Shuna Gould annotation2032826 1 vioE range2032826 1 6770 7345 annotation2032552 1 rbs range2032552 1 1 17 annotation2032822 1 vioC range2032822 1 4312 5607 annotation2032720 1 rbs range2032720 1 1275 1291 annotation2032821 1 rbs range2032821 1 4295 4311 annotation2032721 1 vioB range2032721 1 1292 4294 annotation2032824 1 vioD range2032824 1 5625 6752 annotation2032825 1 rbs range2032825 1 6753 6769 annotation2032668 1 vioA range2032668 1 18 1274 annotation2032823 1 rbs range2032823 1 5608 5624 BBa_K274002_sequence 1 ttaaggaggtaaaaaaaatgaaacattcttccgatatctgcattgttggtgctggtatttctggtttgacgtgcgcaagccatctgctggacagcccggcatgccgtggtctgagcctgcgtatctttgacatgcagcaagaagccggtggccgtatccgcagcaaaatgctggatggtaaggcaagcattgaactgggcgcaggtcgctactcccctcagttgcacccgcatttccaaagcgcaatgcagcactatagccaaaagagcgaagtctatccgttcacccagttgaagttcaaatctcacgtgcagcaaaagctgaagcgcgccatgaatgaactgtccccgcgtctgaaagagcatggtaaagagagctttttgcagtttgtcagccgttatcaaggtcacgatagcgcggttggtatgatccgctctatgggttacgacgcactgttcctgccggatatcagcgcagaaatggcctacgacattgtgggtaagcacccggagatccagagcgtgacggacaacgacgcgaaccaatggtttgcagcggaaacgggctttgctggtctgattcagggcatcaaggctaaggttaaggcggcaggtgcgcgttttagcctgggttatcgtctgctgagcgtccgtaccgacggtgacggctacctgctgcaactggcaggtgacgacggctggaaactggagcaccgtacccgccatctgattctggcgattccgccgagcgcgatggcgggtttgaatgttgattttccagaagcctggtccggtgcgcgctatggcagcctgccgctgtttaagggctttctgacgtacggtgagccgtggtggttggactacaaactggacgatcaggtgctgattgttgacaacccgctgcgcaaaatctatttcaaaggcgataagtacctgttcttctataccgatagcgagatggcgaattactggcgcggttgtgtcgcggagggcgaggacggttacctggagcaaattcgcacccatttggctagcgcactgggtatcgtccgtgaacgtatcccgcaaccgctggcacacgttcacaagtattgggcgcacggcgttgagttttgccgtgattctgatattgaccacccgagcgcactgtctcatcgcgacagcggtatcatcgcgtgctccgatgcgtacacggagcattgtggttggatggagggcggtctgctgagcgcccgtgaggcaagccgtctgctgttgcagcgtatcgccgcgtgattaaggaggtaaaaaaaatgagcattctggatttcccgcgtatccacttccgtggctgggcccgtgtcaatgcgccgaccgcgaaccgcgatccgcacggccacatcgatatggccagcaataccgtggcgatggcgggtgagccgttcgacctggcacgccatcctacggagttccaccgtcacctgcgctccctgggtccgcgcttcggcttggatggtcgtgctgacccggaaggcccgttcagcctggccgagggctacaacgctgccggtaacaaccacttttcgtgggagagcgcaaccgttagccacgtgcaatgggatggcggtgaggcggatcgtggtgacggtctggtcggtgctcgtttggcactgtggggtcactacaatgattatctgcgtaccaccttcaatcgtgctcgttgggtcgacagcgacccgacgcgccgtgacgctgcacaaatctatgcgggccaattcaccattagcccggctggtgccggtccgggtacgccgtggctgtttacggcagacattgatgatagccatggtgcacgttggacgcgtggcggccacattgcagagcgtggcggccacttcttggatgaagagtttggtctggcacgcctgtttcagttctctgtgccgaaagatcacccacattttctgtttcacccgggtccgtttgattccgaggcctggcgtcgtctgcaattggctctggaggatgacgacgttctgggtctgaccgtgcaatatgcgttgttcaatatgagcaccccgcctcagccgaacagcccggtttttcacgatatggtcggtgttgtcggtctgtggcgtcgtggtgaactggcgagctacccggctggtcgtctgctgcgtccgcgtcaaccgggtctgggtgacctgaccctgcgcgtcaacggtggtcgcgttgcgctgaatttggcgtgtgccattccgttcagcactcgtgccgcgcagccaagcgcaccggaccgcctgaccccggacctgggtgccaaactgccgctgggcgatctgctgctgcgtgatgaggacggcgcactgttggcacgtgtgccgcaggctctgtaccaagactattggacgaatcacggtattgtggacctgccgctgctgcgcgaaccgcgtggtagcttgaccctgagcagcgaactggcggagtggcgtgagcaagactgggtcacccaaagcgacgcgtctaacctgtacctggaggcaccggatcgccgtcacggtcgctttttccctgagagcatcgcgctgcgcagctactttcgcggtgaagcgcgtgcgcgtccggatatcccgcatcgtatcgagggcatgggcctggtcggcgtcgaatctcgtcaggatggcgacgctgcggaatggcgtctgacgggtctgcgtccgggtccggcacgcattgttctggacgatggtgccgaggcgatccctctgcgtgttctgcctgacgattgggcgctggatgacgcgaccgtcgaagaagtggattacgcctttttgtaccgccacgttatggcgtattacgagctggtgtatccattcatgagcgacaaggtgttttccctggctgatcgttgcaaatgtgaaacgtacgcacgtctgatgtggcagatgtgtgatccgcagaaccgcaacaagtcctattacatgccgagcacccgcgaactgtcggcaccgaaagctcgtttgttcttgaagtatctggcccacgtggaaggccaggcacgcctgcaagcacctccgccagcgggtccggcacgcattgaatctaaagcccagttggcggcagagctgcgtaaagccgtcgacctggagctgtctgtgatgctgcaatacctgtacgcggcgtatagcattccgaactatgcacagggccaacaacgtgttcgtgacggtgcgtggaccgccgagcagctgcaactggcgtgcggtagcggtgaccgtcgccgtgatggcggtattcgtgcagcactgctggaaattgctcatgaagaaatgattcattacctggtcgttaacaacctgctgatggccctgggcgagccgttctacgcgggtgtcccgctgatgggcgaagcggcacgtcaggcgtttggcctggacaccgagttcgctctggaaccgtttagcgaaagcacgctggcacgttttgttcgtctggaatggccgcactttatcccagcaccgggcaaatccatcgcggactgctatgccgccattcgtcaggcgtttttggatctgccggacttgtttggtggcgaggcaggtaagcgtggcggtgaacaccacctgttcctgaatgagctgaccaaccgtgcgcatccgggttatcaactggaagttttcgatcgcgactcggcgctgtttggtattgcatttgtgaccgatcagggcgaaggtggcgctctggacagcccgcactacgaacatagccattttcaacgtctgcgtgaaatgagcgcgcgtatcatggctcaaagcgcaccgttcgaaccggcgctgccggcgttgcgtaatccggttctggatgagagcccgggttgccaacgtgtcgcagacggtcgtgcgcgtgcgctgatggcattgtaccaaggcgtttatgagctgatgtttgcgatgatggcgcagcacttcgccgtgaaaccgctgggtagcttgcgtcgcagccgcctgatgaacgcagcaatcgatctgatgaccggtctgttgcgtccgctgagctgcgcgctgatgaacctgccaagcggcatcgccggtcgcacggccggtccgccgctgccgggtccggttgacacccgtagctatgacgactacgcgctgggctgtcgcatgctggcacgccgttgcgagcgtctgctggagcaggcgagcatgctggaaccgggttggctgccggatgcgcagatggagctgctggatttctatcgtcgccaaatgctggacttggcgtgcggcaaactgagccgcgaggcctaaggatccttaaggaggtaaaaaaaatgaaacgtgcgattatcgttggtggcggcctggcgggtggcctgaccgcgatctacctggcgaagcgtggctacgaagtgcacgtcgtggagaagcgtggtgatcctctgcgcgatctgagctcttacgtggacgttgttagcagccgtgcgatcggcgtgagcatgaccgttcgtggtatcaagagcgttttggctgcgggcattccgcgtgcagagctggatgcgtgtggcgaaccgatcgtggcaatggctttctccgtgggtggtcagtatcgcatgcgcgaactgaagccgttggaggatttccgtccgctgagcttgaaccgtgcggcgtttcaaaagctgctgaacaaatacgcgaacctggcaggcgttcgttactactttgagcataagtgcctggatgttgacctggatggtaagagcgtgttgattcagggcaaagatggtcagccgcagcgtctgcaaggtgacatgattatcggtgcggatggcgcccacagcgccgtccgtcaggcgatgcagagcggcctgcgtcgtttcgagttccagcaaacgttcttccgccatggctacaaaaccctggttttgccggacgcgcaagcactgggttaccgtaaagacacgctgtactttttcggcatggattccggtggcctgttcgcgggtcgtgcggctacgatcccagatggtagcgtcagcatcgccgtttgcctgccgtactcgggtagcccttccctgacgaccaccgacgaaccgacgatgcgtgcgttcttcgatcgttacttcggtggcctgccgcgtgacgcgcgtgacgaaatgctgcgtcagtttctggcgaagccgagcaacgacctgattaacgtgcgctctagcacctttcactataagggtaatgtgctgttgctgggtgatgctgcgcatgcgactgcgccgttcctgggtcagggtatgaacatggcgctggaggacgcccgcacgtttgtcgagctgctggaccgccaccagggcgaccaagacaaagcctttccggagttcacggagctgcgcaaagtccaggcagacgcaatgcaagacatggctcgcgccaactatgacgttttgagctgctcgaacccgatctttttcatgcgtgcgcgttacacgcgttacatgcattccaagtttccgggcctgtatccgccggatatggccgagaaactgtactttacgagcgagccgtacgatcgtctgcaacaaatccagcgtaaacagaatgtttggtacaagattggtcgcgtgaattgaagatctttaaggaggtaaaaaaaatgaagattctggtcattggtgctggtccagctggtctggttttcgcatcccaactgaagcaggcacgccctttgtgggccattgacatcgtggagaagaatgacgagcaagaagtgctgggctggggtgtcgtgctgcctggccgtccgggtcagcacccggcgaacccgctgtcctatctggatgcaccggagcgtctgaatccgcaatttctggaggacttcaaactggtgcatcataatgagccgtccttgatgtccacgggcgttttgttgtgcggcgtggagcgtcgcggtctggttcacgcgctgcgcgataagtgccgcagccaaggcattgctattcgtttcgaaagcccgttgctggaacacggtgagctgccgctggcggactatgatctggtggtcctggctaatggtgttaatcacaaaaccgcgcatttcaccgaggctctggtcccgcaggtggactacggccgcaataagtacatttggtatggcactagccagctgttcgatcagatgaatctggtttttcgtacccatggtaaagatatctttatcgcgcatgcctataagtatagcgataccatgagcacgttcattgtcgaatgtagcgaagagacttacgcacgcgcacgcctgggcgaaatgtccgaagaggcgagcgcagaatacgttgctaaggtgttccaggccgagctgggtggtcacggcctggtgagccagccgggtctgggttggcgtaacttcatgacgttgtctcatgaccgttgtcatgatggtaagttggttctgctgggtgacgcgctgcaaagcggtcactttagcatcggccacggcaccacgatggccgtggtggtggcgcagctgctggttaaagcgctgtgtaccgaagatggtgtgcctgccgcgctgaaacgtttcgaagagcgtgccctgccgctggtgcagttgttccgtggccacgcagacaacagccgcgtttggttcgaaaccgtcgaagagcgcatgcacctgtcctcggcggaatttgtgcaaagcttcgacgcacgccgcaaaagcctgccgccgatgccggaagcactggcgcagaatctgcgttatgctttgcagcgctgatgatcattaaggaggtaaaaaaaatggagaaccgtgagccaccactgttgccagcccgttggagcagcgcctatgtctcttattggagcccgatgctgccggatgaccagctgaccagcggctattgctggttcgactatgaacgtgacatctgtcgtattgacggcctgttcaatccgtggagcgagcgtgatactggttatcgcctgtggatgtcggaggttggtaatgcggccagcggccgtacctggaaacaaaaagtcgcctatggtcgtgagcgtaccgccctgggtgaacagctgtgtgagcgtccgctggatgatgagactggcccttttgccgaattgttcctgccacgcgatgtcctgcgccgtctgggtgcccgtcacattggccgtcgcgtggttctgggtcgcgaagcggacggttggcgttaccagcgcccaggtaaaggtccgagcaccctgtacctggatgcggcgagcggcactccactgcgcatggtcaccggcgatgaagcgtcgcgtgcaagcctgcgtgattttccgaatgtgagcgaggcggagatcccggacgcggttttcgcggccaagcgctaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z