BBa_K274004 1 BBa_K274004 Vio operon ABCE 2009-10-18T11:00:00Z 2015-05-08T01:11:45Z This part was made from the vio operon biobrick, part number BBa_K274002. The vioD gene has been removed by restriction digest. Produces a light green pigment from L-tyrosine. Formed from the vio operon biobrick (BBa_K274002) with the vioD gene removed by restriction with BglII and BclI. This sequence contains four genes, vioA, vioB vioD and vioE, each preceded by their own ribosome binding site. false false _374_ 0 5412 9 In stock false The origonal vio operon was designed with the vioD gene flanked by complementary sticky end restriction sites for BglII and BclI. This allowed the vioD to be easily removed, leading to the production of the light green pigment. false Shuna Gould annotation2061084 1 rbs range2061084 1 4295 4311 annotation2061082 1 rbs range2061082 1 1275 1291 annotation2061080 1 rbs range2061080 1 1 17 annotation2061081 1 VioA range2061081 1 18 1274 annotation2061085 1 VioC range2061085 1 4312 5607 annotation2061093 1 rbs range2061093 1 5608 5624 annotation2061083 1 VioB range2061083 1 1292 4294 annotation2061095 1 VioE range2061095 1 5625 6200 BBa_K274004_sequence 1 ttaaggaggtaaaaaaaatgaaacattcttccgatatctgcattgttggtgctggtatttctggtttgacgtgcgcaagccatctgctggacagcccggcatgccgtggtctgagcctgcgtatctttgacatgcagcaagaagccggtggccgtatccgcagcaaaatgctggatggtaaggcaagcattgaactgggcgcaggtcgctactcccctcagttgcacccgcatttccaaagcgcaatgcagcactatagccaaaagagcgaagtctatccgttcacccagttgaagttcaaatctcacgtgcagcaaaagctgaagcgcgccatgaatgaactgtccccgcgtctgaaagagcatggtaaagagagctttttgcagtttgtcagccgttatcaaggtcacgatagcgcggttggtatgatccgctctatgggttacgacgcactgttcctgccggatatcagcgcagaaatggcctacgacattgtgggtaagcacccggagatccagagcgtgacggacaacgacgcgaaccaatggtttgcagcggaaacgggctttgctggtctgattcagggcatcaaggctaaggttaaggcggcaggtgcgcgttttagcctgggttatcgtctgctgagcgtccgtaccgacggtgacggctacctgctgcaactggcaggtgacgacggctggaaactggagcaccgtacccgccatctgattctggcgattccgccgagcgcgatggcgggtttgaatgttgattttccagaagcctggtccggtgcgcgctatggcagcctgccgctgtttaagggctttctgacgtacggtgagccgtggtggttggactacaaactggacgatcaggtgctgattgttgacaacccgctgcgcaaaatctatttcaaaggcgataagtacctgttcttctataccgatagcgagatggcgaattactggcgcggttgtgtcgcggagggcgaggacggttacctggagcaaattcgcacccatttggctagcgcactgggtatcgtccgtgaacgtatcccgcaaccgctggcacacgttcacaagtattgggcgcacggcgttgagttttgccgtgattctgatattgaccacccgagcgcactgtctcatcgcgacagcggtatcatcgcgtgctccgatgcgtacacggagcattgtggttggatggagggcggtctgctgagcgcccgtgaggcaagccgtctgctgttgcagcgtatcgccgcgtgattaaggaggtaaaaaaaatgagcattctggatttcccgcgtatccacttccgtggctgggcccgtgtcaatgcgccgaccgcgaaccgcgatccgcacggccacatcgatatggccagcaataccgtggcgatggcgggtgagccgttcgacctggcacgccatcctacggagttccaccgtcacctgcgctccctgggtccgcgcttcggcttggatggtcgtgctgacccggaaggcccgttcagcctggccgagggctacaacgctgccggtaacaaccacttttcgtgggagagcgcaaccgttagccacgtgcaatgggatggcggtgaggcggatcgtggtgacggtctggtcggtgctcgtttggcactgtggggtcactacaatgattatctgcgtaccaccttcaatcgtgctcgttgggtcgacagcgacccgacgcgccgtgacgctgcacaaatctatgcgggccaattcaccattagcccggctggtgccggtccgggtacgccgtggctgtttacggcagacattgatgatagccatggtgcacgttggacgcgtggcggccacattgcagagcgtggcggccacttcttggatgaagagtttggtctggcacgcctgtttcagttctctgtgccgaaagatcacccacattttctgtttcacccgggtccgtttgattccgaggcctggcgtcgtctgcaattggctctggaggatgacgacgttctgggtctgaccgtgcaatatgcgttgttcaatatgagcaccccgcctcagccgaacagcccggtttttcacgatatggtcggtgttgtcggtctgtggcgtcgtggtgaactggcgagctacccggctggtcgtctgctgcgtccgcgtcaaccgggtctgggtgacctgaccctgcgcgtcaacggtggtcgcgttgcgctgaatttggcgtgtgccattccgttcagcactcgtgccgcgcagccaagcgcaccggaccgcctgaccccggacctgggtgccaaactgccgctgggcgatctgctgctgcgtgatgaggacggcgcactgttggcacgtgtgccgcaggctctgtaccaagactattggacgaatcacggtattgtggacctgccgctgctgcgcgaaccgcgtggtagcttgaccctgagcagcgaactggcggagtggcgtgagcaagactgggtcacccaaagcgacgcgtctaacctgtacctggaggcaccggatcgccgtcacggtcgctttttccctgagagcatcgcgctgcgcagctactttcgcggtgaagcgcgtgcgcgtccggatatcccgcatcgtatcgagggcatgggcctggtcggcgtcgaatctcgtcaggatggcgacgctgcggaatggcgtctgacgggtctgcgtccgggtccggcacgcattgttctggacgatggtgccgaggcgatccctctgcgtgttctgcctgacgattgggcgctggatgacgcgaccgtcgaagaagtggattacgcctttttgtaccgccacgttatggcgtattacgagctggtgtatccattcatgagcgacaaggtgttttccctggctgatcgttgcaaatgtgaaacgtacgcacgtctgatgtggcagatgtgtgatccgcagaaccgcaacaagtcctattacatgccgagcacccgcgaactgtcggcaccgaaagctcgtttgttcttgaagtatctggcccacgtggaaggccaggcacgcctgcaagcacctccgccagcgggtccggcacgcattgaatctaaagcccagttggcggcagagctgcgtaaagccgtcgacctggagctgtctgtgatgctgcaatacctgtacgcggcgtatagcattccgaactatgcacagggccaacaacgtgttcgtgacggtgcgtggaccgccgagcagctgcaactggcgtgcggtagcggtgaccgtcgccgtgatggcggtattcgtgcagcactgctggaaattgctcatgaagaaatgattcattacctggtcgttaacaacctgctgatggccctgggcgagccgttctacgcgggtgtcccgctgatgggcgaagcggcacgtcaggcgtttggcctggacaccgagttcgctctggaaccgtttagcgaaagcacgctggcacgttttgttcgtctggaatggccgcactttatcccagcaccgggcaaatccatcgcggactgctatgccgccattcgtcaggcgtttttggatctgccggacttgtttggtggcgaggcaggtaagcgtggcggtgaacaccacctgttcctgaatgagctgaccaaccgtgcgcatccgggttatcaactggaagttttcgatcgcgactcggcgctgtttggtattgcatttgtgaccgatcagggcgaaggtggcgctctggacagcccgcactacgaacatagccattttcaacgtctgcgtgaaatgagcgcgcgtatcatggctcaaagcgcaccgttcgaaccggcgctgccggcgttgcgtaatccggttctggatgagagcccgggttgccaacgtgtcgcagacggtcgtgcgcgtgcgctgatggcattgtaccaaggcgtttatgagctgatgtttgcgatgatggcgcagcacttcgccgtgaaaccgctgggtagcttgcgtcgcagccgcctgatgaacgcagcaatcgatctgatgaccggtctgttgcgtccgctgagctgcgcgctgatgaacctgccaagcggcatcgccggtcgcacggccggtccgccgctgccgggtccggttgacacccgtagctatgacgactacgcgctgggctgtcgcatgctggcacgccgttgcgagcgtctgctggagcaggcgagcatgctggaaccgggttggctgccggatgcgcagatggagctgctggatttctatcgtcgccaaatgctggacttggcgtgcggcaaactgagccgcgaggcctaaggatccttaaggaggtaaaaaaaatgaaacgtgcgattatcgttggtggcggcctggcgggtggcctgaccgcgatctacctggcgaagcgtggctacgaagtgcacgtcgtggagaagcgtggtgatcctctgcgcgatctgagctcttacgtggacgttgttagcagccgtgcgatcggcgtgagcatgaccgttcgtggtatcaagagcgttttggctgcgggcattccgcgtgcagagctggatgcgtgtggcgaaccgatcgtggcaatggctttctccgtgggtggtcagtatcgcatgcgcgaactgaagccgttggaggatttccgtccgctgagcttgaaccgtgcggcgtttcaaaagctgctgaacaaatacgcgaacctggcaggcgttcgttactactttgagcataagtgcctggatgttgacctggatggtaagagcgtgttgattcagggcaaagatggtcagccgcagcgtctgcaaggtgacatgattatcggtgcggatggcgcccacagcgccgtccgtcaggcgatgcagagcggcctgcgtcgtttcgagttccagcaaacgttcttccgccatggctacaaaaccctggttttgccggacgcgcaagcactgggttaccgtaaagacacgctgtactttttcggcatggattccggtggcctgttcgcgggtcgtgcggctacgatcccagatggtagcgtcagcatcgccgtttgcctgccgtactcgggtagcccttccctgacgaccaccgacgaaccgacgatgcgtgcgttcttcgatcgttacttcggtggcctgccgcgtgacgcgcgtgacgaaatgctgcgtcagtttctggcgaagccgagcaacgacctgattaacgtgcgctctagcacctttcactataagggtaatgtgctgttgctgggtgatgctgcgcatgcgactgcgccgttcctgggtcagggtatgaacatggcgctggaggacgcccgcacgtttgtcgagctgctggaccgccaccagggcgaccaagacaaagcctttccggagttcacggagctgcgcaaagtccaggcagacgcaatgcaagacatggctcgcgccaactatgacgttttgagctgctcgaacccgatctttttcatgcgtgcgcgttacacgcgttacatgcattccaagtttccgggcctgtatccgccggatatggccgagaaactgtactttacgagcgagccgtacgatcgtctgcaacaaatccagcgtaaacagaatgtttggtacaagattggtcgcgtgaattgaagatcattaaggaggtaaaaaaaatggagaaccgtgagccaccactgttgccagcccgttggagcagcgcctatgtctcttattggagcccgatgctgccggatgaccagctgaccagcggctattgctggttcgactatgaacgtgacatctgtcgtattgacggcctgttcaatccgtggagcgagcgtgatactggttatcgcctgtggatgtcggaggttggtaatgcggccagcggccgtacctggaaacaaaaagtcgcctatggtcgtgagcgtaccgccctgggtgaacagctgtgtgagcgtccgctggatgatgagactggcccttttgccgaattgttcctgccacgcgatgtcctgcgccgtctgggtgcccgtcacattggccgtcgcgtggttctgggtcgcgaagcggacggttggcgttaccagcgcccaggtaaaggtccgagcaccctgtacctggatgcggcgagcggcactccactgcgcatggtcaccggcgatgaagcgtcgcgtgcaagcctgcgtgattttccgaatgtgagcgaggcggagatcccggacgcggttttcgcggccaagcgctaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z