LOCUS       BBa_K274100             3385 bp    DNA     linear       22-Dec-2024
DEFINITION  CrtEBI with rbs
ACCESSION   BBa_K274100
VERSION     BBa_K274100.1  
FEATURES             Location/Qualifiers
     RBS             1..4
                     /label=BBa_K118012
     CDS             11..922
                     /label=BBa_I742151
     misc_feature    931..933
                     /label=SacI
     RBS             934..940
                     /label=rbs
     RBS             931..940
                     /label=BBa_J15001
     CDS             947..1876
                     /label=crtB coding sequence
     CDS             947..1879
                     /label=BBa_K118002
     misc_feature    1888..1890
                     /label=SacI
     RBS             1891..1897
                     /label=rbs
     RBS             1888..1897
                     /label=BBa_J15001
     misc_feature    2381..2386
                     /label=PstI site removed
     misc_feature    2792..2797
                     /label=PstI site removed
     CDS             1904..3382
                     /label=crtI coding sequence
     CDS             1904..3385
                     /label=BBa_K118003
ORIGIN
        1 gaggtactag atgacggtct gcgcaaaaaa acacgttcat ctcactcgcg atgctgcgga
       61 gcagttactg gctgatattg atcgacgcct tgatcagtta ttgcccgtgg agggagaacg
      121 ggatgttgtg ggtgccgcga tgcgtgaagg tgcgctggca ccgggaaaac gtattcgccc
      181 catgttgctg ttgctgaccg cccgcgatct gggttgcgct gtcagccatg acggattact
      241 ggatttggcc tgtgcggtgg aaatggtcca cgcggcttcg ctgatccttg acgatatgcc
      301 ctgcatggac gatgcgaagc tgcggcgcgg acgccctacc attcattctc attacggaga
      361 gcatgtggca atactggcgg cggttgcctt gctgagtaaa gcctttggcg taattgccga
      421 tgcagatggc ctcacgccgc tggcaaaaaa tcgggcggtt tctgaactgt caaacgccat
      481 cggcatgcaa ggattggttc agggtcagtt caaggatctg tctgaagggg ataagccgcg
      541 cagcgctgaa gctattttga tgacgaatca ctttaaaacc agcacgctgt tttgtgcctc
      601 catgcagatg gcctcgattg ttgcgaatgc ctccagcgaa gcgcgtgatt gcctgcatcg
      661 tttttcactt gatcttggtc aggcatttca actgctggac gatttgaccg atggcatgac
      721 cgacaccggt aaggatagca atcaggacgc cggtaaatcg acgctggtca atctgttagg
      781 cccgagggcg gttgaagaac gtctgagaca acatcttcag cttgccagtg agcatctctc
      841 tgcggcctgc caacacgggc acgccactca acattttatt caggcctggt ttgacaaaaa
      901 actcgctgcc gtcagttaat aatactagag ctcaaggagg tactagatga ataatccgtc
      961 gttactcaat catgcggtcg aaacgatggc agttggctcg aaaagttttg cgacagcctc
     1021 aaagttattt gatgcaaaaa cccggcgcag cgtactgatg ctctacgcct ggtgccgcca
     1081 ttgtgacgat gttattgacg atcagacgct gggctttcag gcccggcagc ctgccttaca
     1141 aacgcccgaa caacgtctga tgcaacttga gatgaaaacg cgccaggcct atgcaggatc
     1201 gcagatgcac gaaccggcgt ttgcggcttt tcaggaagtg gctatggctc atgatatcgc
     1261 cccggcttac gcgtttgatc atctggaagg cttcgccatg gatgtacgcg aagcgcaata
     1321 cagccaactg gatgatacgc tgcgctattg ctatcacgtt gcaggcgttg tcggcttgat
     1381 gatggcgcaa atcatgggcg tgcgggataa cgccacgctg gaccgcgcct gtgaccttgg
     1441 gctggcattt cagttgacca atattgctcg cgatattgtg gacgatgcgc atgcgggccg
     1501 ctgttatctg ccggcaagct ggctggagca tgaaggtctg aacaaagaga attatgcggc
     1561 acctgaaaac cgtcaggcgc tgagccgtat cgcccgtcgt ttggtgcagg aagcagaacc
     1621 ttactatttg tctgccacag ccggcctggc agggttgccc ctgcgttccg cctgggcaat
     1681 cgctacggcg aagcaggttt accggaaaat aggtgtcaaa gttgaacagg ccggtcagca
     1741 agcctgggat cagcggcagt caacgaccac gcccgaaaaa ttaacgctgc tgctggccgc
     1801 ctctggtcag gcccttactt cccggatgcg ggctcatcct ccccgccctg cgcatctctg
     1861 gcagcgcccg ctctaataat actagagctc aaggaggtac tagatgaaac caactacggt
     1921 aattggtgca ggcttcggtg gcctggcact ggcaattcgt ctacaagctg cggggatccc
     1981 cgtcttactg cttgaacaac gtgataaacc cggcggtcgg gcttatgtct acgaggatca
     2041 ggggtttacc tttgatgcag gcccgacggt tatcaccgat cccagtgcca ttgaagaact
     2101 gtttgcactg gcaggaaaac agttaaaaga gtatgtcgaa ctgctgccgg ttacgccgtt
     2161 ttaccgcctg tgttgggagt cagggaaggt ctttaattac gataacgatc aaacccggct
     2221 cgaagcgcag attcagcagt ttaatccccg cgatgtcgaa ggttatcgtc agtttctgga
     2281 ctattcacgc gcggtgttta aagaaggcta tctaaagctc ggtactgtcc cttttttatc
     2341 gttcagagac atgcttcgcg ccgcacctca actggcgaaa ctgcaagcat ggagaagcgt
     2401 ttacagtaag gttgccagtt acatcgaaga tgaacatctg cgccaggcgt tttctttcca
     2461 ctcgctgttg gtgggcggca atcccttcgc cacctcatcc atttatacgt tgatacacgc
     2521 gctggagcgt gagtggggcg tctggtttcc gcgtggcggc accggcgcat tagttcaggg
     2581 gatgataaag ctgtttcagg atctgggtgg cgaagtcgtg ttaaacgcca gagtcagcca
     2641 tatggaaacg acaggaaaca agattgaagc cgtgcattta gaggacggtc gcaggttcct
     2701 gacgcaagcc gtcgcgtcaa atgcagatgt ggttcatacc tatcgcgacc tgttaagcca
     2761 gcaccctgcc gcggttaagc agtccaacaa actgcaaact aagcgcatga gtaactctct
     2821 gtttgtgctc tattttggtt tgaatcacca tcatgatcag ctcgcgcatc acacggtttg
     2881 tttcggcccg cgttaccgcg agctgattga cgaaattttt aatcatgatg gcctcgcaga
     2941 ggacttctca ctttatctgc acgcgccctg tgtcacggat tcgtcactgg cgcctgaagg
     3001 ttgcggcagt tactatgtgt tggcgccggt gccgcattta ggcaccgcga acctcgactg
     3061 gacggttgag gggccaaaac tacgcgaccg tatttttgcg taccttgagc agcattacat
     3121 gcctggctta cggagtcagc tggtcacgca ccggatgttt acgccgtttg attttcgcga
     3181 ccagcttaat gcctatcatg gctcagcctt ttctgtggag cccgttctta cccagagcgc
     3241 ctggtttcgg ccgcataacc gcgataaaac cattactaat ctctacctgg tcggcgcagg
     3301 cacgcatccc ggcgcaggca ttcctggcgt catcggctcg gcaaaagcga cagcaggttt
     3361 gatgctggag gatctgatat aataa
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