LOCUS BBa_K274220 5773 bp DNA linear 14-Nov-2024 DEFINITION CrtEBIY under pBad promoter ACCESSION BBa_K274220 VERSION BBa_K274220.1 FEATURES Location/Qualifiers promoter 1080..1210 /label=Pbad promoter 1043..1114 /label=PC misc_feature 1..1079 /label=AraC promoter 1..1210 /label=BBa_I0500 RBS 1219..1222 /label=BBa_K118012 CDS 1229..2140 /label=BBa_I742151 misc_feature 2149..2151 /label=SacI RBS 2152..2158 /label=rbs RBS 2149..2158 /label=BBa_J15001 CDS 2165..3094 /label=crtB coding sequence CDS 2165..3097 /label=BBa_K118002 misc_feature 3106..3108 /label=SacI RBS 3109..3115 /label=rbs RBS 3106..3115 /label=BBa_J15001 misc_feature 3599..3604 /label=PstI site removed misc_feature 4010..4015 /label=PstI site removed CDS 3122..4600 /label=crtI coding sequence CDS 3122..4603 /label=BBa_K118003 RBS 4612..4615 /label=BBa_K118012 CDS 4622..5770 /label=crtY coding sequence CDS 4622..5773 /label=BBa_K118008 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc tactagagga ggtactagat gacggtctgc gcaaaaaaac acgttcatct 1261 cactcgcgat gctgcggagc agttactggc tgatattgat cgacgccttg atcagttatt 1321 gcccgtggag ggagaacggg atgttgtggg tgccgcgatg cgtgaaggtg cgctggcacc 1381 gggaaaacgt attcgcccca tgttgctgtt gctgaccgcc cgcgatctgg gttgcgctgt 1441 cagccatgac ggattactgg atttggcctg tgcggtggaa atggtccacg cggcttcgct 1501 gatccttgac gatatgccct gcatggacga tgcgaagctg cggcgcggac gccctaccat 1561 tcattctcat tacggagagc atgtggcaat actggcggcg gttgccttgc tgagtaaagc 1621 ctttggcgta attgccgatg cagatggcct cacgccgctg gcaaaaaatc gggcggtttc 1681 tgaactgtca aacgccatcg gcatgcaagg attggttcag ggtcagttca aggatctgtc 1741 tgaaggggat aagccgcgca gcgctgaagc tattttgatg acgaatcact ttaaaaccag 1801 cacgctgttt tgtgcctcca tgcagatggc ctcgattgtt gcgaatgcct ccagcgaagc 1861 gcgtgattgc ctgcatcgtt tttcacttga tcttggtcag gcatttcaac tgctggacga 1921 tttgaccgat ggcatgaccg acaccggtaa ggatagcaat caggacgccg gtaaatcgac 1981 gctggtcaat ctgttaggcc cgagggcggt tgaagaacgt ctgagacaac atcttcagct 2041 tgccagtgag catctctctg cggcctgcca acacgggcac gccactcaac attttattca 2101 ggcctggttt gacaaaaaac tcgctgccgt cagttaataa tactagagct caaggaggta 2161 ctagatgaat aatccgtcgt tactcaatca tgcggtcgaa acgatggcag ttggctcgaa 2221 aagttttgcg acagcctcaa agttatttga tgcaaaaacc cggcgcagcg tactgatgct 2281 ctacgcctgg tgccgccatt gtgacgatgt tattgacgat cagacgctgg gctttcaggc 2341 ccggcagcct gccttacaaa cgcccgaaca acgtctgatg caacttgaga tgaaaacgcg 2401 ccaggcctat gcaggatcgc agatgcacga accggcgttt gcggcttttc aggaagtggc 2461 tatggctcat gatatcgccc cggcttacgc gtttgatcat ctggaaggct tcgccatgga 2521 tgtacgcgaa gcgcaataca gccaactgga tgatacgctg cgctattgct atcacgttgc 2581 aggcgttgtc ggcttgatga tggcgcaaat catgggcgtg cgggataacg ccacgctgga 2641 ccgcgcctgt gaccttgggc tggcatttca gttgaccaat attgctcgcg atattgtgga 2701 cgatgcgcat gcgggccgct gttatctgcc ggcaagctgg ctggagcatg aaggtctgaa 2761 caaagagaat tatgcggcac ctgaaaaccg tcaggcgctg agccgtatcg cccgtcgttt 2821 ggtgcaggaa gcagaacctt actatttgtc tgccacagcc ggcctggcag ggttgcccct 2881 gcgttccgcc tgggcaatcg ctacggcgaa gcaggtttac cggaaaatag gtgtcaaagt 2941 tgaacaggcc ggtcagcaag cctgggatca gcggcagtca acgaccacgc ccgaaaaatt 3001 aacgctgctg ctggccgcct ctggtcaggc ccttacttcc cggatgcggg ctcatcctcc 3061 ccgccctgcg catctctggc agcgcccgct ctaataatac tagagctcaa ggaggtacta 3121 gatgaaacca actacggtaa ttggtgcagg cttcggtggc ctggcactgg caattcgtct 3181 acaagctgcg gggatccccg tcttactgct tgaacaacgt gataaacccg gcggtcgggc 3241 ttatgtctac gaggatcagg ggtttacctt tgatgcaggc ccgacggtta tcaccgatcc 3301 cagtgccatt gaagaactgt ttgcactggc aggaaaacag ttaaaagagt atgtcgaact 3361 gctgccggtt acgccgtttt accgcctgtg ttgggagtca gggaaggtct ttaattacga 3421 taacgatcaa acccggctcg aagcgcagat tcagcagttt aatccccgcg atgtcgaagg 3481 ttatcgtcag tttctggact attcacgcgc ggtgtttaaa gaaggctatc taaagctcgg 3541 tactgtccct tttttatcgt tcagagacat gcttcgcgcc gcacctcaac tggcgaaact 3601 gcaagcatgg agaagcgttt acagtaaggt tgccagttac atcgaagatg aacatctgcg 3661 ccaggcgttt tctttccact cgctgttggt gggcggcaat cccttcgcca cctcatccat 3721 ttatacgttg atacacgcgc tggagcgtga gtggggcgtc tggtttccgc gtggcggcac 3781 cggcgcatta gttcagggga tgataaagct gtttcaggat ctgggtggcg aagtcgtgtt 3841 aaacgccaga gtcagccata tggaaacgac aggaaacaag attgaagccg tgcatttaga 3901 ggacggtcgc aggttcctga cgcaagccgt cgcgtcaaat gcagatgtgg ttcataccta 3961 tcgcgacctg ttaagccagc accctgccgc ggttaagcag tccaacaaac tgcaaactaa 4021 gcgcatgagt aactctctgt ttgtgctcta ttttggtttg aatcaccatc atgatcagct 4081 cgcgcatcac acggtttgtt tcggcccgcg ttaccgcgag ctgattgacg aaatttttaa 4141 tcatgatggc ctcgcagagg acttctcact ttatctgcac gcgccctgtg tcacggattc 4201 gtcactggcg cctgaaggtt gcggcagtta ctatgtgttg gcgccggtgc cgcatttagg 4261 caccgcgaac ctcgactgga cggttgaggg gccaaaacta cgcgaccgta tttttgcgta 4321 ccttgagcag cattacatgc ctggcttacg gagtcagctg gtcacgcacc ggatgtttac 4381 gccgtttgat tttcgcgacc agcttaatgc ctatcatggc tcagcctttt ctgtggagcc 4441 cgttcttacc cagagcgcct ggtttcggcc gcataaccgc gataaaacca ttactaatct 4501 ctacctggtc ggcgcaggca cgcatcccgg cgcaggcatt cctggcgtca tcggctcggc 4561 aaaagcgaca gcaggtttga tgctggagga tctgatataa taatactaga ggaggtacta 4621 gatgcaaccg cattatgatc tgattctcgt gggggctgga ctcgcgaatg gccttatcgc 4681 cctgcgtctt cagcagcagc aacctgatat gcgtattttg cttatcgacg ccgcacccca 4741 ggcgggcggg aatcatacgt ggtcatttca ccacgatgat ttgactgaga gccaacatcg 4801 ttggatagct ccgctggtgg ttcatcactg gcccgactat caggtacgct ttcccacacg 4861 ccgtcgtaag ctgaacagcg gctacttttg tattacttct cagcgtttcg ctgaggtttt 4921 acagcgacag tttggcccgc acttgtggat ggataccgcg gtcgcagagg ttaatgcgga 4981 atctgttcgg ttgaaaaagg gtcaggttat cggtgcccgc gcggtgattg acgggcgggg 5041 ttatgcggca aattcagcac tgagcgtggg cttccaggcg tttattggcc aggaatggcg 5101 attgagccac ccgcatggtt tatcgtctcc cattatcatg gatgccacgg tcgatcagca 5161 aaatggttat cgcttcgtgt acagcctgcc gctctcgccg accagattgt taattgaaga 5221 cacgcactat attgataatg cgacattaga tcctgaatgc gcgcggcaaa atatttgcga 5281 ctatgccgcg caacagggtt ggcagcttca gacactgctg cgagaagaac agggcgcctt 5341 acccattact ctgtcgggca atgccgacgc attctggcag cagcgccccc tggcctgtag 5401 tggattacgt gccggtctgt tccatcctac caccggctat tcactgccgc tggcggttgc 5461 cgtggccgac cgcctgagtg cacttgatgt ctttacgtcg gcctcaattc accatgccat 5521 tacgcatttt gcccgcgagc gctggcagca gcagggcttt ttccgcatgc tgaatcgcat 5581 gctgttttta gccggacccg ccgattcacg ctggcgggtt atgcagcgtt tttatggttt 5641 acctgaagat ttaattgccc gtttttatgc gggaaaactc acgctgaccg atcggctacg 5701 tattctgagc ggcaagccgc ctgttccggt attagcagca ttgcaagcca ttatgacgac 5761 tcatcgttaa taa //