LOCUS       BBa_K274392              501 bp    DNA     linear       21-May-2024
DEFINITION  PoPS -> PhiR73 pag - PP promoter -> PoPS
ACCESSION   BBa_K274392
VERSION     BBa_K274392.1  
FEATURES             Location/Qualifiers
     misc_feature    1..12
                     /label=B0034
     CDS             22..264
                     /label=phiR73 delta
     misc_feature    22..22
                     /label=phiR73 delta
     misc_feature    102..102
                     /label=silent mutation to remove PstI site
     misc_feature    1..264
                     /label=BBa_I746352
     stem_loop       284..327
                     /label=stem_loop
     misc_feature    273..352
                     /label=BBa_B0010
     terminator      273..352
                     /label=BBa_B0010
     stem_loop       368..387
                     /label=T7 TE
     misc_feature    394..394
                     /label=stop
     polyA_site      388..401
                     /label=polya
     misc_feature    361..401
                     /label=BBa_B0012
     terminator      361..401
                     /label=BBa_B0012
     promoter        410..501
                     /label=PP
     promoter        410..501
                     /label=BBa_I746362
ORIGIN
        1 aaagaggaga aatactagat gatgcgctgc cctttctgtc gtcattcagc gcatacccgc
       61 accagccggt atgtgagtga caatgtcaaa gaaagttatc tccagtgcca gaatatttac
      121 tgttcggcga catttaaaac gcatgagtca atttgtgccg tgattcgttc tccggtcacg
      181 gaggaaaaac cagcaccggc aagcacagca ccggctgttg tccgaaaagt taaaggctgt
      241 tacagctcac cattcaacca ttaatactag agccaggcat caaataaaac gaaaggctca
      301 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag
      361 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagaga cggcaaggct
      421 actgagtcgc gccccgcgat tcgctaaggt gctgttgtgt cagtgataag ccatccggga
      481 ctgatggcgg aggatgcgca t
//