BBa_K274392
1
BBa_K274392
PoPS -> PhiR73 pag - PP promoter -> PoPS
2009-10-18T11:00:00Z
2015-05-08T01:11:45Z
n/a
n/a
false
false
_374_
0
5448
9
Not in stock
false
n/a
false
Vivian Mullin
component2055393
1
BBa_B0010
component2055395
1
BBa_B0012
component2055400
1
BBa_I746362
component2055392
1
BBa_I746352
annotation2055400
1
BBa_I746362
range2055400
1
410
501
annotation2055393
1
BBa_B0010
range2055393
1
273
352
annotation2055395
1
BBa_B0012
range2055395
1
361
401
annotation2055392
1
BBa_I746352
range2055392
1
1
264
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation4184
1
stem_loop
range4184
1
12
55
annotation7018
1
BBa_B0010
range7018
1
1
80
BBa_I746362
1
BBa_I746362
PP promoter from P2 phage
2007-09-10T11:00:00Z
2015-08-31T04:08:04Z
P2 phage genome
This is the PP promoter taken from the P2 phage genome. It is an inducible promoter that is activated by a class of activators, including P2 ogr (I746350), PSP3 pag (I746351) and phiR73 delta (I746352). These different activators should cause different levels of activity of the PP promoter.
false
false
_116_
0
2122
9
It's complicated
false
no special considerations
true
Stefan Milde
annotation1943785
1
PP
range1943785
1
1
92
BBa_I746352
1
BBa_I746352
delta activator from phiR73 phage
2007-09-11T11:00:00Z
2015-08-31T04:08:04Z
plasmid DNA supplied by Prof. Richard Calendar, University of California.
The delta activator taken from phiR73 phage acts on a class of inducible promoters (parts I746360 to I746365), inducing their activity to varying degrees.
The part sequence does already contain a ribosome binding site (B0034)!
false
false
_116_
0
2122
9
In stock
true
The part does contain a RBS (B0034) already.
true
Stefan Milde
annotation1943872
1
phiR73 delta
range1943872
1
22
264
annotation1943871
1
B0034
range1943871
1
1
12
annotation1943873
1
phiR73 delta
range1943873
1
22
22
annotation1943874
1
silent mutation to remove PstI site
range1943874
1
102
102
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1690
1
polya
range1690
1
28
41
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1687
1
stop
range1687
1
34
34
annotation1686
1
T7 TE
range1686
1
8
27
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_I746362_sequence
1
acggcaaggctactgagtcgcgccccgcgattcgctaaggtgctgttgtgtcagtgataagccatccgggactgatggcggaggatgcgcat
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K274392_sequence
1
aaagaggagaaatactagatgatgcgctgccctttctgtcgtcattcagcgcatacccgcaccagccggtatgtgagtgacaatgtcaaagaaagttatctccagtgccagaatatttactgttcggcgacatttaaaacgcatgagtcaatttgtgccgtgattcgttctccggtcacggaggaaaaaccagcaccggcaagcacagcaccggctgttgtccgaaaagttaaaggctgttacagctcaccattcaaccattaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacggcaaggctactgagtcgcgccccgcgattcgctaaggtgctgttgtgtcagtgataagccatccgggactgatggcggaggatgcgcat
BBa_I746352_sequence
1
aaagaggagaaatactagatgatgcgctgccctttctgtcgtcattcagcgcatacccgcaccagccggtatgtgagtgacaatgtcaaagaaagttatctccagtgccagaatatttactgttcggcgacatttaaaacgcatgagtcaatttgtgccgtgattcgttctccggtcacggaggaaaaaccagcaccggcaagcacagcaccggctgttgtccgaaaagttaaaggctgttacagctcaccattcaaccattaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z