LOCUS BBa_K277000 3002 bp DNA linear 22-Nov-2024 DEFINITION pGEM-T Cloning Vector ACCESSION BBa_K277000 VERSION BBa_K277000.1 FEATURES Location/Qualifiers misc_feature 2960..3001 /label=Multiple cloning site RBS 2951..2959 /label=T7 RNA polymerase transcription initiation site misc_feature 2..61 /label=Multiple cloning site CDS 1261..2121 /label=beta-lactamase (Ampr) ORIGIN 1 tatcactagt gcggccgcct gcaggtcgac catatgggag agctcccaac gcgttggatg 61 catagcttga gtattctata gtgtcaccta aatagcttgg cgtaatcatg gtcatagctg 121 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata 181 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca 241 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc 301 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg 361 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta 421 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc 481 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag 541 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac 601 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc 661 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt 721 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc 781 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga 841 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta 901 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta 961 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga 1021 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg 1081 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag 1141 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc 1201 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact 1261 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt 1321 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta 1381 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta 1441 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc 1501 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat 1561 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt 1621 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg 1681 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca 1741 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta 1801 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg 1861 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact 1921 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg 1981 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt 2041 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga 2101 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc 2161 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa 2221 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgatgcggt gtgaaatacc 2281 gcacagatgc gtaaggagaa aataccgcat caggaaattg taagcgttaa tattttgtta 2341 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc 2401 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg 2461 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat 2521 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc 2581 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag 2641 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg 2701 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta 2761 cagggcgcgt ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg 2821 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg 2881 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa ttgtaatacg 2941 actcactata gggcgaattg ggcccgacgt cgcatgctcc cggccgccat ggccgcggga 3001 ta //