BBa_K277016 1 BBa_K277016 3L.3_23.A2.04 2009-10-19T11:00:00Z 2015-05-08T01:11:45Z Synthetic Yeast 3L.3_23.A2.04 is 743 bases long and is cloned into the pGem-T vector. 3L.3_23.A2.04 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.A2.04 is a constituent of 3L.3_23.A2 (along with 3L.3_23.A2.01, 3L.3_23.A2.02, 3L.3_23.A2.03, 3L.3_23.A2.05, 3L.3_23.A2.06, 3L.3_23.A2.07, 3L.3_23.A2.08, 3L.3_23.A2.09, 3L.3_23.A2.10, 3L.3_23.A2.11, 3L.3_23.A2.12, 3L.3_23.A2.13, and 3L.3_23.A2.14.) This part contains at least part of the following features (positions offset from first base of sequence): kind and name offset notes reverse_primer YCL061C_tagr2v1 (607..634) reverse_primer YCL061C_tagr1v1 (400..427) gene YCL061C (-2120..+1170) S-phase checkpoint protein found at replication forks%2C required for DNA replication%3B also required for Rad53p activation during DNA replication stress%2C where it forms a replication-pausing complex with Tof1p and is phosphorylated by Mec1p%3B protein involved in replication checkpoint forward_primer YCL061C_tagf1v1 (7..34) forward_primer YCL061C_tagf2v1 (136..163) Sequence (the first 743 bases correspond to coordinates 11072..11814 in synthetic chromosome yeast_chr3_3_23) false false _385_ 0 2669 9 It's complicated false Constructed using overlap extension PCR protocol described in RFC38 false James DiCarlo BBa_K277016_sequence 1 agcatagctagatagcaaagggactcgcttttcgtttattcttgtctcatcagttagatttaattcaacacttttgttctcatgcagttcatcgtcattctgtactggttttggatgtgcattttccagctttatgtcgtcatcttcgttgctgctgctgtcatcaaaatcagcaagaaaagactctttagaaaaaaattttttggttggtattggcttttgcggttttttgtattcgacatttctagctttttcccttttagaatcttctgaagccaattcgctttgtatttcccgaatttttaatgtgtgtggtacttcatcttgaagtgtttgtgcgactgtatctatttgggttttctgttcctgatgcgtagcctgtggaataccaattgcttgggttttgtcttcgtcgtgagcggtttgaggtggtacatccatttgggtgtcaggaccacgattttgaatggactgaaataaaggactagttccaataagttccgttggaattataggaattttcggcgacagagcattagaatgattgctggaagtcgtaattatttgggattgtggttcaattgattttataggctgagtttgtgaagtagcgccagaagagtcgattcgttgggtagcccctggccgtcctgtcgtgagaggtgttttcagcgcttgagaagtttcgttgacgagaccatcttctttgattaattgggtgggtttcccgtcattaacggaat igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z