BBa_K277072 1 BBa_K277072 3L.3_23.C2.02 2009-10-20T11:00:00Z 2015-05-08T01:11:46Z Synthetic Yeast 3L.3_23.C2.02 is 734 bases long and is cloned into the pGem-T vector. 3L.3_23.C2.02 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. The whole synthetic chromosome may be viewed at http://macbeth.clark.jhu.edu/cgi-bin/gbrowse 3L.3_23.C2.02 is a constituent of 3L.3_23.C2 (along with 3L.3_23.C2.01, 3L.3_23.C2.03, 3L.3_23.C2.04, 3L.3_23.C2.05, 3L.3_23.C2.06, 3L.3_23.C2.07, 3L.3_23.C2.08, and 3L.3_23.C2.09.) This part contains at least part of the following features (positions offset from first base of sequence): kind and name offset notes gene YCL034W (-488..576) Protein of unknown function%3B binds Las17p%2C which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization loxP_site loxPsym_YCL034W (580..613) gene YCL033C (664..+1170) Putative protein-methionine-R-oxide reductase%3B involved in response to oxidative stress%3B similar to mouse Sepx1p and fly SelRp%3B YCL033C is not an essential gene mutation_affecting_coding_sequence YCL034W_re_remove_BglI (10..21) removal of BglI reverse_primer YCL034W_tagr1v1 (477..504) forward_primer YCL034W_tagf1v1 (168..189) Sequence (corresponds to coordinates 52656..53389 in synthetic chromosome yeast_chr3_3_23) false false _385_ 0 2669 9 It's complicated false Constructed using overlap extension PCR protocol described in RFC38 false James DiCarlo BBa_K277072_sequence 1 agccgttccaggcgtagcggcggaggctctggcggacggtctaacttcatggatgatagcgccgacgacactttgtaccagtccaactcgctgactagtgccgatagattgtataggattccacagatcaacatgaacaaagaggcacccaggattcggctcataatttctgacgccttggctagcgccgtgtcgttacagaactcgctgatcgggctgcccaagggcaagttctccacagacgatgaagaggccacgtccaagttcatccaggcaagggcgatcagaaggaaagttcttagatatctgcaattggtcacggagggtgagtttctgggtagcttaattcatgccaatgatgaactggtggctgcgttgactgcatacgacgaccgaagcgctcaggacgatagcagcgatgaaagtgatcacggctcgtacgatgacggtatttatgatgaaaacgagcaggataattctcgatacatcgactctgaaagcagcgaggaagagagcttgtcttcttatcagccgtccacgatctcaaaccctttcggtgatcataacaaaatttgaagaataacttcgtataatgtacattatacgaagttatagtatgtacgtatgcatacacacatatatatatatatatatatagtacatttaatccttcttgaggtttaaagacgcactgttcacacagtgtctggtgtccttgggcaagtttagcaact igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z