BBa_K277087 1 BBa_K277087 3L.3_23.C3.08 2009-10-20T11:00:00Z 2015-05-08T01:11:46Z Synthetic Yeast 3L.3_23.C3.08 is 669 bases long and is cloned into the pGem-T vector. 3L.3_23.C3.08 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. The whole synthetic chromosome may be viewed at http://macbeth.clark.jhu.edu/cgi-bin/gbrowse 3L.3_23.C3.08 is a constituent of 3L.3_23.C3 (along with 3L.3_23.C3.01, 3L.3_23.C3.02, 3L.3_23.C3.03, 3L.3_23.C3.04, 3L.3_23.C3.05, 3L.3_23.C3.06, 3L.3_23.C3.07, 3L.3_23.C3.09, 3L.3_23.C3.10, 3L.3_23.C3.11, 3L.3_23.C3.12, 3L.3_23.C3.13, and 3L.3_23.C3.14.) This part contains at least part of the following features (positions offset from first base of sequence): kind and name offset notes gene YCL026C-B (658..+1239) Putative protein of unknown function%3B green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus gene YCL027W (-978..560) Membrane protein localized to the shmoo tip%2C required for cell fusion%3B expression regulated by mating pheromone%3B proposed to coordinate signaling%2C fusion%2C and polarization events required for fusion%3B potential Cdc28p substrate loxP_site loxPsym_YCL027W (564..597) forward_primer YCL027W_tagf2v1 (68..95) reverse_primer YCL027W_tagr2v1 (266..293) Sequence (corresponds to coordinates 63461..64129 in synthetic chromosome yeast_chr3_3_23) false false _385_ 0 2669 9 It's complicated false Constructed using overlap extension PCR protocol described in RFC38 false James DiCarlo BBa_K277087_sequence 1 acagtgtgaataacttgggtaataccacgccagattcacaaatcacatcttatcgcaacaataacattgacttgattaccgctcgaccacacagcgtgatatacggtactactgcacaacaaactttggaaaccaacttcaatgatcatcatgactgcaataaaagcactgagaaacacgagttgataatacccaccccatcaaaaccactaaagaaaaggaaaaaaagaagacaaagtaaaatgtatcagcatttacaacatttaagccgaagcaagcctctaccattgaccccaaactccaaatataatggggaggctagcgtccaattagggaagacatatacagttattcaggattacgagcctagattgacagacgaaataagaatctcgctgggtgaaaaagttaaaattctggccactcataccgatggatggtgtctggtagaaaagtgtaatacacaaaagggttctattcacgtcagtgttgacgataaaagatacctcaatgaagatagaggcattgtgcctggtgactgtctccaagaatacgactgatgaataacttcgtataatgtacattatacgaagttataaataatattgacgttcgcatttaatctatacctataattctgtacttatatactgttccttaattgaagat igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z