BBa_K277003 1 BBa_K277003 3L.3_23.A1.03 2009-10-19T11:00:00Z 2015-05-08T01:11:45Z synthetic Yeast 3L.3_23.A1.03 is 779 bases long and is cloned into the pGem-T vector. 3L.3_23.A1.03 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.A1.03 is a constituent of 3L.3_23.A1 (along with 3L.3_23.A1.01, 3L.3_23.A1.02, 3L.3_23.A1.04, 3L.3_23.A1.05, 3L.3_23.A1.06, 3L.3_23.A1.07, 3L.3_23.A1.08, 3L.3_23.A1.09, 3L.3_23.A1.10, 3L.3_23.A1.11, and 3L.3_23.A1.12.) This part contains at least part of the following features (positions offset from first base of sequence): kind and name offset notes gene YCL073C (-1042..+805) Protein of unconfirmed function%3B displays a topology characteristic of the Major Facilitators Superfamily of membrane proteins%3B coding sequence 98% identical to that of YKR106W Sequence (the first 779 bases correspond to coordinates 1456..2234 in synthetic chromosome yeast_chr3_3_23) false false _385_ 0 2669 9 It's complicated false Constructed using overlap extension PCR proto descibed in RFC38 false James DiCarlo BBa_K277011 1 BBa_K277011 3L.3_23.A1.11 2009-10-19T11:00:00Z 2015-05-08T01:11:45Z Synthetic Yeast 3L.3_23.A1.11 is 656 bases long and is cloned into the pGem-T vector. 3L.3_23.A1.11 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.A1.11 is a constituent of 3L.3_23.A1 (along with 3L.3_23.A1.01, 3L.3_23.A1.02, 3L.3_23.A1.03, 3L.3_23.A1.04, 3L.3_23.A1.05, 3L.3_23.A1.06, 3L.3_23.A1.07, 3L.3_23.A1.08, 3L.3_23.A1.09, 3L.3_23.A1.10, and 3L.3_23.A1.12.) This part contains at least part of the following features (positions offset from first base of sequence): kind and name offset notes gene YCL063W (-80..+1191) Protein involved in vacuole inheritance%3B acts as a vacuole-specific receptor for myosin Myo2p forward_primer YCL063W_tagf1v1 (534..561) Sequence (the first 656 bases correspond to coordinates 7438..8093 in synthetic chromosome yeast_chr3_3_23) false false _385_ 0 2669 9 It's complicated false Constructed using overlap extension PCR protocol described in RFC38 false James DiCarlo BBa_K277009 1 BBa_K277009 3L.3_23.A1.09 2009-10-19T11:00:00Z 2015-05-08T01:11:45Z Synthetic Yeast 3L.3_23.A1.09 is 744 bases long and is cloned into the pGem-T vector. 3L.3_23.A1.09 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.A1.09 is a constituent of 3L.3_23.A1 (along with 3L.3_23.A1.01, 3L.3_23.A1.02, 3L.3_23.A1.03, 3L.3_23.A1.04, 3L.3_23.A1.05, 3L.3_23.A1.06, 3L.3_23.A1.07, 3L.3_23.A1.08, 3L.3_23.A1.10, 3L.3_23.A1.11, and 3L.3_23.A1.12.) This part contains at least part of the following features (positions offset from first base of sequence): kind and name offset notes ARS ARS320 (-345..462) Autonomously Replicating Sequence on Chromosome III forward_primer YCL064C_tagf1v1 (94..121) reverse_primer YCL064C_tagr1v1 (337..364) gene YCL064C (-14..+1068) Catabolic L-serine (L-threonine) deaminase%2C catalyzes the degradation of both L-serine and L-threonine%3B required to use serine or threonine as the sole nitrogen source%2C transcriptionally induced by serine and threonine Sequence (the first 744 bases correspond to coordinates 5880..6623 in synthetic chromosome yeast_chr3_3_23) false false _385_ 0 2669 9 It's complicated false Constructed using overlap extension PCR protocol described in RFC38 false James DiCarlo BBa_K277006 1 BBa_K277006 3L.3_23.A1.06 2009-10-19T11:00:00Z 2015-05-08T01:11:45Z synthetic Yeast 3L.3_23.A1.06 is 754 bases long and is cloned into the pGem-T vector. 3L.3_23.A1.06 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.A1.06 is a constituent of 3L.3_23.A1 (along with 3L.3_23.A1.01, 3L.3_23.A1.02, 3L.3_23.A1.03, 3L.3_23.A1.04, 3L.3_23.A1.05, 3L.3_23.A1.07, 3L.3_23.A1.08, 3L.3_23.A1.09, 3L.3_23.A1.10, 3L.3_23.A1.11, and 3L.3_23.A1.12.) This part contains at least part of the following features (positions offset from first base of sequence): kind and name offset notes gene YCL069W (-43..+1333) Permease of basic amino acids in the vacuolar membrane Sequence (the first 754 bases correspond to coordinates 3716..4469 in synthetic chromosome yeast_chr3_3_23): false false _385_ 0 2669 9 It's complicated false Constructed using overlap extension PCR protocol described in RFC38 false James DiCarlo BBa_K277134 1 BBa_K277134 3L.3_23.A1 2009-10-20T11:00:00Z 2015-05-08T01:11:47Z Synthetic Yeast 3L.3_23.A1 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns,repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.A1 is comprised of and must be constructed from the parts 3L.3_23.A1.01, 3L.3_23.A1.02, 3L.3_23.A1.03, 3L.3_23.A1.04, 3L.3_23.A1.05, 3L.3_23.A1.06, 3L.3_23.A1.07, 3L.3_23.A1.08, 3L.3_23.A1.09, 3L.3_23.A1.10, 3L.3_23.A1.11, and 3L.3_23.A1.12. This part wholly contains the following features (positions offset from first base of sequence): kind and name offset notes loxP_site loxpsym_delyeast103_25(chrIII)3000..3001 (3000..3033) gene YCL073C (413..2260) Protein of unconfirmed function%3B displays a topology characteristic of the Major Facilitators Superfamily of membrane proteins%3B coding sequence 98% identical to that of YKR106W loxP_site loxPsym_YCL063W (8632..8665) gene YCL069W (3672..5048) Permease of basic amino acids in the vacuolar membrane ARS ARS320 (5534..6341) Autonomously Replicating Sequence on Chromosome III loxP_site loxpsym_delyeast_chr3_3_17(chrIII)5500..9500 (5500..5533) reverse_primer YCL063W_tagr1v1 (8223..8250) loxP_site loxpsym_delyeast103_24(chrIII)1..6100 (1..34) mutation_affecting_coding_sequence YCL069W_re_remove_AlwNI (4680..4691) removal of AlwNI forward_primer YCL064C_tagf1v1 (5973..6000) loxP_site loxPsym_YCL069W (5052..5085) gene YCL063W (7357..8628) Protein involved in vacuole inheritance%3B acts as a vacuole-specific receptor for myosin Myo2p forward_primer YCL063W_tagf1v1 (7971..7998) reverse_primer YCL064C_tagr1v1 (6216..6243) ARS ARS301 (5146..5401) Inactive replication origin associated with the silent mating type locus HML%2C where it functions as a transcriptional silencer gene YCL064C (5865..6947) Catabolic L-serine (L-threonine) deaminase%2C catalyzes the degradation of both L-serine and L-threonine%3B required to use serine or threonine as the sole nitrogen source%2C transcriptionally induced by serine and threonine Sequence (corresponding to coordinates 1..8879 in synthetic chromosome yeast_chr3_3_23) false false _385_ 0 2669 9 It's complicated false Constructed using USER assembly method described in RFC38 false James DiCarlo component2061981 1 BBa_K277003 component2061989 1 BBa_K277011 component2061982 1 BBa_K277004 component2061984 1 BBa_K277006 component2061988 1 BBa_K277010 component2061985 1 BBa_K277007 component2061990 1 BBa_K277012 component2061979 1 BBa_K277001 component2061987 1 BBa_K277009 component2061986 1 BBa_K277008 component2061983 1 BBa_K277005 component2061980 1 BBa_K277002 annotation2061987 1 BBa_K277009 range2061987 1 6018 6761 annotation2061990 1 BBa_K277012 range2061990 1 8274 8771 annotation2061981 1 BBa_K277003 range2061981 1 1492 2270 annotation2061988 1 BBa_K277010 range2061988 1 6770 7601 annotation2061984 1 BBa_K277006 range2061984 1 3803 4556 annotation2061979 1 BBa_K277001 range2061979 1 1 744 annotation2061985 1 BBa_K277007 range2061985 1 4565 5253 annotation2061980 1 BBa_K277002 range2061980 1 753 1483 annotation2061982 1 BBa_K277004 range2061982 1 2279 3035 annotation2061989 1 BBa_K277011 range2061989 1 7610 8265 annotation2061983 1 BBa_K277005 range2061983 1 3044 3794 annotation2061986 1 BBa_K277008 range2061986 1 5262 6009 BBa_K277012 1 BBa_K277012 3L.3_23.A1.12 2009-10-19T11:00:00Z 2015-05-08T01:11:45Z Synthetic Yeast 3L.3_23.A1.12 is 795 bases long and is cloned into the pGem-T vector. 3L.3_23.A1.12 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.A1.12 is a constituent of 3L.3_23.A1 (along with 3L.3_23.A1.01, 3L.3_23.A1.02, 3L.3_23.A1.03, 3L.3_23.A1.04, 3L.3_23.A1.05, 3L.3_23.A1.06, 3L.3_23.A1.07, 3L.3_23.A1.08, 3L.3_23.A1.09, 3L.3_23.A1.10, and 3L.3_23.A1.11.) This part contains at least part of the following features (positions offset from first base of sequence): kind and name offset notes loxP_site loxPsym_YCL063W (548..581) reverse_primer YCL063W_tagr1v1 (139..166) gene YCL063W (-727..544) Protein involved in vacuole inheritance%3B acts as a vacuole-specific receptor for myosin Myo2p Sequence (the first 795 bases correspond to coordinates 8085..8879 in synthetic chromosome yeast_chr3_3_23) false false _385_ 0 2669 9 It's complicated false Constructed using overlap extension PCR protocol described in RFC38 false James DiCarlo BBa_K277001 1 BBa_K277001 3L.3_23.A1.01 2009-10-19T11:00:00Z 2015-05-08T01:11:45Z Synthetic Yeast 3L.3_23.A1.01 is 744 bases long and is cloned into the pGem-T vector. 3L.3_23.A1.01 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.A1.01 is a constituent of 3L.3_23.A1 (along with 3L.3_23.A1.02, 3L.3_23.A1.03, 3L.3_23.A1.04, 3L.3_23.A1.05, 3L.3_23.A1.06, 3L.3_23.A1.07, 3L.3_23.A1.08, 3L.3_23.A1.09, 3L.3_23.A1.10, 3L.3_23.A1.11, and 3L.3_23.A1.12.) This part contains at least part of the following features (positions offset from first base of sequence): kind and name offset notes gene YCL073C (413..+2260) Protein of unconfirmed function%3B displays a topology characteristic of the Major Facilitators Superfamily of membrane proteins%3B coding sequence 98% identical to that of YKR106W loxP_site loxpsym_delyeast103_24(chrIII)1..6100 (1..34) Sequence (the first 744 bases correspond to coordinates 1..744 in synthetic chromosome yeast_chr3_3_23) false false _385_ 0 2669 9 It's complicated false Built with overlap extension PCR false James DiCarlo BBa_K277010 1 BBa_K277010 3L.3_23.A1.10 2009-10-19T11:00:00Z 2015-05-08T01:11:45Z Synthetic Yeast 3L.3_23.A1.10 is 832 bases long and is cloned into the pGem-T vector. 3L.3_23.A1.10 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.A1.10 is a constituent of 3L.3_23.A1 (along with 3L.3_23.A1.01, 3L.3_23.A1.02, 3L.3_23.A1.03, 3L.3_23.A1.04, 3L.3_23.A1.05, 3L.3_23.A1.06, 3L.3_23.A1.07, 3L.3_23.A1.08, 3L.3_23.A1.09, 3L.3_23.A1.11, and 3L.3_23.A1.12.) This part contains at least part of the following features (positions offset from first base of sequence): kind and name offset notes gene YCL063W (743..+2014) Protein involved in vacuole inheritance%3B acts as a vacuole-specific receptor for myosin Myo2p gene YCL064C (-749..333) Catabolic L-serine (L-threonine) deaminase%2C catalyzes the degradation of both L-serine and L-threonine%3B required to use serine or threonine as the sole nitrogen source%2C transcriptionally induced by serine and threonine Sequence (the first 832 bases correspond to coordinates 6615..7446 in synthetic chromosome yeast_chr3_3_23) false false _385_ 0 2669 9 It's complicated false Constructed using overlap extension PCR protocol described in RFC38 false James DiCarlo BBa_K277007 1 BBa_K277007 3L.3_23.A1.07 2009-10-19T11:00:00Z 2015-05-08T01:11:45Z synthetic Yeast 3L.3_23.A1.07 is 689 bases long and is cloned into the pGem-T vector. 3L.3_23.A1.07 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.A1.07 is a constituent of 3L.3_23.A1 (along with 3L.3_23.A1.01, 3L.3_23.A1.02, 3L.3_23.A1.03, 3L.3_23.A1.04, 3L.3_23.A1.05, 3L.3_23.A1.06, 3L.3_23.A1.08, 3L.3_23.A1.09, 3L.3_23.A1.10, 3L.3_23.A1.11, and 3L.3_23.A1.12.) This part contains at least part of the following features (positions offset from first base of sequence): kind and name offset notes gene YCL069W (-788..588) Permease of basic amino acids in the vacuolar membrane mutation_affecting_coding_sequence YCL069W_re_remove_AlwNI (220..231) removal of AlwNI loxP_site loxPsym_YCL069W (592..625) ARS ARS301 (686..+941) Inactive replication origin associated with the silent mating type locus HML%2C where it functions as a transcriptional silencer Sequence (the first 689 bases correspond to coordinates 4461..5149 in synthetic chromosome yeast_chr3_3_23) false false _385_ 0 2669 9 It's complicated false Constructed using overlap extension PCR protocol described in RFC38 false James DiCarlo BBa_K277008 1 BBa_K277008 3L.3_23.A1.08 2009-10-19T11:00:00Z 2015-05-08T01:11:45Z synthetic Yeast 3L.3_23.A1.08 is 748 bases long and is cloned into the pGem-T vector. 3L.3_23.A1.08 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.A1.08 is a constituent of 3L.3_23.A1 (along with 3L.3_23.A1.01, 3L.3_23.A1.02, 3L.3_23.A1.03, 3L.3_23.A1.04, 3L.3_23.A1.05, 3L.3_23.A1.06, 3L.3_23.A1.07, 3L.3_23.A1.09, 3L.3_23.A1.10, 3L.3_23.A1.11, and 3L.3_23.A1.12.) This part contains at least part of the following features (positions offset from first base of sequence): kind and name offset notes ARS ARS320 (394..+1201) Autonomously Replicating Sequence on Chromosome III loxP_site loxpsym_delyeast_chr3_3_17(chrIII)5500..9500 (360..393) ARS ARS301 (6..261) Inactive replication origin associated with the silent mating type locus HML%2C where it functions as a transcriptional silencer gene YCL064C (725..+1807) Catabolic L-serine (L-threonine) deaminase%2C catalyzes the degradation of both L-serine and L-threonine%3B required to use serine or threonine as the sole nitrogen source%2C transcriptionally induced by serine and threonine Sequence (the first 748 bases correspond to coordinates 5141..5888 in synthetic chromosome yeast_chr3_3_23) false false _385_ 0 2669 9 It's complicated false Constructed using overlap extension PCR protocol described in RFC38 false James DiCarlo BBa_K277005 1 BBa_K277005 3L.3_23.A1.05 2009-10-19T11:00:00Z 2015-05-08T01:11:45Z synthetic Yeast 3L.3_23.A1.05 is 751 bases long and is cloned into the pGem-T vector. 3L.3_23.A1.05 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.A1.05 is a constituent of 3L.3_23.A1 (along with 3L.3_23.A1.01, 3L.3_23.A1.02, 3L.3_23.A1.03, 3L.3_23.A1.04, 3L.3_23.A1.06, 3L.3_23.A1.07, 3L.3_23.A1.08, 3L.3_23.A1.09, 3L.3_23.A1.10, 3L.3_23.A1.11, and 3L.3_23.A1.12.) This part contains at least part of the following features (positions offset from first base of sequence): kind and name offset notes loxP_site loxpsym_delyeast103_25(chrIII)3000..3001 (27..60) gene YCL069W (699..+2075) Permease of basic amino acids in the vacuolar membrane Sequence (the first 751 bases correspond to coordinates 2974..3724 in synthetic chromosome yeast_chr3_3_23) false false _385_ 0 2669 9 It's complicated false Constructed using overlap extension PCR protocol described in RFC38 false James DiCarlo BBa_K277002 1 BBa_K277002 3L.3_23.A1.02 2009-10-19T11:00:00Z 2015-05-08T01:11:45Z Synthetic Yeast 3L.3_23.A1.02 is 731 bases long and is cloned into the pGem-T vector. 3L.3_23.A1.02 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.A1.02 is a constituent of 3L.3_23.A1 (along with 3L.3_23.A1.01, 3L.3_23.A1.03, 3L.3_23.A1.04, 3L.3_23.A1.05, 3L.3_23.A1.06, 3L.3_23.A1.07, 3L.3_23.A1.08, 3L.3_23.A1.09, 3L.3_23.A1.10, 3L.3_23.A1.11, and 3L.3_23.A1.12.) This part contains at least part of the following features (positions offset from first base of sequence): kind and name offset notes gene YCL073C (-320..+1527) Protein of unconfirmed function%3B displays a topology characteristic of the Major Facilitators Superfamily of membrane proteins%3B coding sequence 98% identical to that of YKR106W Sequence (the first 731 bases correspond to coordinates 734..1464 in synthetic chromosome yeast_chr3_3_23) false false _385_ 0 2669 9 It's complicated false Assembled by Overlap extension PCR false James DiCarlo BBa_K277004 1 BBa_K277004 3L.3_23.A1.04 2009-10-19T11:00:00Z 2015-05-08T01:11:45Z synthetic Yeast 3L.3_23.A1.04 is 757 bases long and is cloned into the pGem-T vector. 3L.3_23.A1.04 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.A1.04 is a constituent of 3L.3_23.A1 (along with 3L.3_23.A1.01, 3L.3_23.A1.02, 3L.3_23.A1.03, 3L.3_23.A1.05, 3L.3_23.A1.06, 3L.3_23.A1.07, 3L.3_23.A1.08, 3L.3_23.A1.09, 3L.3_23.A1.10, 3L.3_23.A1.11, and 3L.3_23.A1.12.) This part contains at least part of the following features (positions offset from first base of sequence): kind and name offset notes gene YCL073C (-1814..33) Protein of unconfirmed function%3B displays a topology characteristic of the Major Facilitators Superfamily of membrane proteins%3B coding sequence 98% identical to that of YKR106W Sequence (the first 757 bases correspond to coordinates 2228..2984 in synthetic chromosome yeast_chr3_3_23) false false _385_ 0 2669 9 It's complicated false Constructed using overlap extension PCR protocol described in RFC38 false James DiCarlo BBa_K277134_sequence 1 ataacttcgtataatgtacattatacgaagttattctttctttttcggtacaacataattaatgcattcatacagagcgcatttccagcatcatttattggatagtaactcctcccgcactttcaatatcaatgacgctgactcttttactgacgctgagtctttaccgacgctgagtctttaccgacgctgagtctttaccgacgctgagtctttaccgacgcttgtgaaaataaaaagtacgacacagctatatgcgtcgcaagttcagatgtgggtctatcgagcatgtttatcctatcctgttatgtcatttttatcggaagtaataaaagtgtagaaatcacttgtttttgccttttttgtgattttgctttcatttttttttgcccttttttcaccagcttggggatcaacatcctaatttcttgttacttcgattatgtctaaaaagtgccttgatttgagagaaaatattttcctcatctggcaagacaaccaaaccatcttcagtgaattcttcgtgtgtcgccttgtctattagttccgggtctctcatgaacattgtaaacgcaaagaacggtaccgtacatgccaagccaaccgtcattattattcgttgaacatatctgtaagattcaaccacagcatctctttcgggtgagccccaaggataatcactaatgaatgtataaggtgatgcatatgctatttctgccaatgtatcgtttccaaggttcttgttactagagaaggttcttgtagagttggttaggcatggtttgtgtccatatggcaccagaaacggaggcaccgatagcagcgcctagctttgaaaaggtatactggatgccggttattacagccatcctactatgcgtagtcatggcttgcagtatgacgatcactgaattgctgcataggagaccactcaaacccatgataacagatgcagcgataacaccttcatgagacccagatcctcctctgtatttgtaaaaaaggcccatgcacaccatccatgcagcacaacctccgattacagaaagtttcagtttccttgtctttgccaccaacaaactgtagaatggagatgcagtagcagcaacaaagtcaggaaggtttactatccgagcagccgaagtggacgattctttcatcgataccagcaaaacaggatacagatagtcacacgaaatgaaaaaggtgaaaaaattgaaaaaagtaacaccaaggggtgcccaaattccacgatcacttagtaatttgaacggtagaagaggagatttggcaaatttggcctcccaatataaaaaaatgaaaaataaacagccacctgaaactaaagtggcaattatttttgaattgtgccacttctgtgatgtctcattagccaacgtcaaagggacaaggatacaccctagcgacacagttattaataatatgccaacaatatcgagtttccagaatagaaacaccaaattactagagacaccaaattctcaaataatccgcccgttctttcctttctagcctgttctttgagagatctccactcagcagtctttgaagatttgtacttcatataaagaataagaaatataattggcaaggcagagagtgggtaaataaaagcccacattgcaatattccaggaccagtttttctgaggatttgctgctgtgataatattacctgaaatccatggtattatgatatatggccaatatgaggcgtactggtaaaacattctccacttcaaggaggagaaatcagaaagtattaatgtcaggagcagatttgttccgacgtatccacagttatagaaaaccgatcctgctgcatacattgtgagacgggtcgcctgtgattgaatgatggttcccattatataaaaaatagttgcaactaaaaaaagccttagtcttccgaagtggtcagagagtctggagtagacaacttgggatccgacacttacaacagcattgataacttggacagttgaaagtaaggagtgttctgaatatgagttcgtcgcatagcccgtataggtcgatctaagtgtgtagtctaaactaattccaaacccacatacaaacgcggtacttatcagtagaattttatatttcaaggaatcaaactgtgcagacataatttcgttttctttgagcttaaaaaaggtcgatgtcatagagtacgtgtcatcgtttgagtgacgttctcgctcaataatctcacaggactgcctaattccactttttttattactttactagagattacttgaggcaccaacaacactagaactcatccgataatttatttacttgattattccttttttttttttttctctttccagtccataaaactattttacttttcttatttatgtaaatagtatttaagattctacggtcgctacatctgctagaaatggaattactttaatacattccatgttctcatatatacttttactgacacctttcgtcactattttttgtttaactttttttttggaagtactaaaattatatgctcgtaattattgagtatcaaaaaaaaaaaaaaaaaaaattgctgtgacaccccttcaatgtggtgtctatacacctggtgactttaactttaactttgacctgtcgtcgtacaatcaacttagtttccgaaaatccacgacgaaccagttataccgaagacttttgtacgacaagaatactgtaccgtcgaaactatctcttttcaaactgatgagtgaagcttgtgtttatgtctgttgaaacaaggaaaaaaatgcggtgcctgtcgacaggatatgcgcacaagtgcaaaatcatcaaaaaattgagtacctagatccggtggtgtaggacgagcaaagttccttcataaacaaaccgtaagggttactgatacacaatttcctttttgtaaagagtatttgagcaaattagtcagcgattgaaaacaacacccttttttctatatatagtaggtgaaagttaacatgcgagtaaaagtagtgccacatttactagagagtgccacatttcctttgcagcaggtataacttcgtataatgtacattatacgaagttataaattggtacatatttaacagatagcgccgcacctcatatgcagcgcgtacatgatacgagacgacaagatatgcaaaagataatagtgtcatcacacctttatgagaagcgaatttttttttttttttggtttaaatatatatatatacatatatatagatatagatatacacatgtatagatgtattctagttatgcttatacctagaactgatcagaccagatcatacctggtatagagcttaatcgatagaaattcaaaaatatggaggaaactaagtactcttcgcagcaggagatagaagaagcatgtggttcagacgcttcattgaatgctagaggtagcaatgattctccaatgggactttccttgtacctctgcctggcttcgtgaactcttgtactattcataactgcactggatattttgatagtgggaactattattgacgtggtcgcagaacagttcggaaactactccaaaacaggttggctcgttacaggctacagtttaccaaatgctattctgagtctcatttggggaagattcgcatctatcataggtttccagcatagtctcattttagcaatacttatttttgaagccggatccctaattgctgcccttgcctcttcaatgaatatgctcattgtcggtagagttgttgctagtgttgggggaagcggacttactagagaagcggacttcaaacgctttgctttgttattggttgtacgatggttggtgaaaggtcacgtccattggtgatttccatcctaagttgtgcatttgctgtagctgctatcgttggtcctataatcggaggtgcctttacaacccatgttacctggaggtggtgcttctatatcaatcttcctatcggtggtcttgccattattatgtttttactcacatataaggccgagaataagggtatacttcaacaaattaaagatgctataggaacaatctcgagctttacttttagtaagttcagacaccaagttaattttaaaagacttatgaatggcataatcttcaagtttgacttctttggttttgccctctgctctgcagggctggtccttttcctactggggctaacctttggtggtaataaatatagttggaactctggccaagtcatcgcatatttggttttgggtgtcttactttttattttttcattggtgtacgatttcttcttattcgataaattcaacccggaacctgataatatatcctacaggcctctccttctaagaagattggtagcaaaaccagccataataataataaacatggtaacatttctattatgtaccggttacaatgggcaaatgatatactctgtccagtttttccaacttatatttgcgtcgagtgcatggaaagccggtcttcacttgataccaatcgttattaccaacgttattgcggccattgcaagttactagagattgcaagtggtgtgattaccaaaaagctcggtttagttaaaccactcttaatatttggaggcgttcttggggtaattggagcagggcttatgacacttatgacaaatacgtccacgaagtcaactcaaattggtgttttgctattaccggggttttcccttggatttgctctacaagcatcgctcatgagtgcacagcttcaaattaccaaagatcgtccagaagccgctatggactttattgaagtaacagctttcaatacattcatgaagtcattaggtacaactcttggtggtgtgctttcaaccactgttttttccgcctcctttcacaacaaagtatcacgagctcatctagagccttacgaaggaaaaacggttgatgacatgattttgtatcgtcttcaaaactacgacggttctcattcgactattggaaacattttaagcgactccattaagaacgtattttggatggatctagggttttatgccttaggatttttgttttgtagtttttcatccaataagaaattaatcataccaaaaaaggacgagacaccagaagataatttagaagacaagtaacgcataacttcgtataatgtacattatacgaagttatagttattttcttattttcattttatttttttcgccttttatacagacttcaacacaatcagaattactagagaatcagaatcaaataggtgtatcgcaatggaatgtaatttcttaagtattctatatgtacttaaaacctattaatatatggatcaacacagtatcttatgaatgggtttttgatttttttatgtttttttaaaacattaaagttttcggcacggacttatttggaattcaaattattaatgaaagaacaattaactaattaatgtacttagtatttggccattattatcgatttcgggggccaaatctaaccaaattcaacctacattttttcaaattgattcaaacacctttcacaataagatttttatatctagcgcacatagaatgaaatgtaaacaaagatttcagaaaaatcgtataacttcgtataatgtacattatacgaagttatgaaattataagttaaaaagcagtagtttatgctttatgctcgagtatcaagtgaatttgaacaggctagtgcttcattggtacttctttcatggataattttgagcaaatttctgcagcatgtccccctttatacaaattctgtgcattgccggcctagaaatatgtcaacgttttggatatgttgatgcttacttcgagaaatcttacactaatacttctggaaaaaatcaatactagcaaaatagtgatatatgagtaaaatgtatgtagtacatgtatgaaaattatcaagggcaaattgatgcttcaacgaaaaagttattggattttcaagcactttttaaattcacaattactagagattcacaatatttttttctggaaatatgaaattgtcagcgacttctattacaggagtgtctttttttctcatgctatccaacgcttcttccaaatctttgatggtgttgctgctaccaccgcaagcaataattatcacaatgtcatccgcagctaattttgagcccagtgcattttctaggatcttagtgttgtaacccaaatgcaatgcggcgccacatgccggttcaatcaccatattgaattgatgtgtatatttaagacaggtttcaataacatctttgtcctctattacaacggatctggtgttgtatttgcgagcgtattcgaaagtttggttgctaataacagcggtacccaagctggtagcaatacttgttatcttcttgaattgaactggttggcctattttcaaagaagtattgaaaacatgacatccattcgtttccacccccacaatagggatcctatcagctaaaccatacctttccaaaccttgaataataccattgtataaaccacctccaccaacgctgcatactatgcctttaaccttattcacggaaatatgttgcgatttcaaatcttgtactatttcatctatcatagatgaatgtccttcccaaatatccggattatcgaagggatgaacataaatgggctcaatgacctgagagtctattttattcatgacatttgtttttaaaaaagtatctgcttctttccagtaggcaccactcacgattactagagactcacgataacctgggcaccggtgttcctgattttatctaccattctcttctttgtcgctgtaggaaccacgactgtacatggtagagacagtctttgacatgctgttgcagcagcaaaaccggcattaccgccagaactagcgaaaacctgaggagatcttttaccgtccttttgaattcgaatggcacttttcatgatgagattaccgattcctctacttttgaaggagccacttggttgaaggcattcatatttcaagaaaaattgtgcagaagcctttccggggaagaattgacgtaataatggtgttttattgtagactatcgacatctcgctggttaattttcctgtctcttgtctatccagcacttaaaaaagagaaaactgtataaacattttccttttatttggcgatatttatatacctgtgttcctcctcatattgtcccttttatcttatctcatcgcaaacaatttgataaagctttggcactatttaatgacactcagtggaattacatttccgctggggcttaacaggagccgcccatgcggaatcatgtctccgcgcagaggactcattagtttccgttcgcggtgattagtactcgcgaaaagagaaaaagaaaaatagtaggaacatttcgcaattgatcaaaatgtaaacagtgcagtaaagaatgggaccataagatagataagaaacagctcgcataaggaaacaaggacacatcgattaatggcaacccaagccctagaggatatcacggagaggcttttaataaggtcgcaagaggctatcttgcaattagatctgtggatacaacgttactagagatacaacgtcagcagagatcatcaatatgccagacaacagatcaagagtcattggataagttatcccaacagtacaaccagtatatgtctcaactgaactctttgtatgttagatcggaatctgttcgagacaagttgagcaaggaacaacaacgcagattgatcacagaggataatgagcatcaacgcatagaagacttggttcgtgaattccaggatatcactttgaggttgaacgagctggccaccgtcccaaatgaagcgcctaatgattctccacaatcgcaatccaccagaagtagtttagggtcatttcaacctcgaccattgaaaataattgagaggcaacgtctgtgtatggtaactccatcgaaaccaccaaaaaaatcggtaggctttaaccccatcaatgaagtcgattgtccttcgaaaactaactctttaccgtgctcacctaaaaaacaacctgcaagaaatcgcactttacgtgcagccaaatcacatgatactggcttgaacaaaagtaagaaaccaagcagcagcgacacctacgaaagctttttcaaaaatagacaaagactttcgttgaccttctttgatgaaatggatgatgaagattttgattctgatcaagatactatcattctaccaaacattactagagatcagatacacatacggtaaaagagaacaacacaaatcttaagcatgcatcttttatggataagtttaattcatcgttaagcacaatatcagaatcttttcaaagtaagagggggagaaagaataagggcatgaatgaagaacgaatatcaaatcataatgtagcacaggaacaaaaaaataatatggatataagcgtctctatagaagaattgcaagatgctttgaatacagaactgctgttttaattaataacttcgtataatgtacattatacgaagttatatgcctcctttacctaacctactcttcttttgctccaaatgtttattctttttttttttattcagaatatcttcaagtttttttgtatctttttgtattattattaaacttctttataatatttgtcttataaaatcctatcataacatgactatggcttggcctagactcgggtgccatcttttttaatgcgactacttcaagacagcttctg BBa_K277007_sequence 1 attgcaagtggtgtgattaccaaaaagctcggtttagttaaaccactcttaatatttggaggcgttcttggggtaattggagcagggcttatgacacttatgacaaatacgtccacgaagtcaactcaaattggtgttttgctattaccggggttttcccttggatttgctctacaagcatcgctcatgagtgcacagcttcaaattaccaaagatcgtccagaagccgctatggactttattgaagtaacagctttcaatacattcatgaagtcattaggtacaactcttggtggtgtgctttcaaccactgttttttccgcctcctttcacaacaaagtatcacgagctcatctagagccttacgaaggaaaaacggttgatgacatgattttgtatcgtcttcaaaactacgacggttctcattcgactattggaaacattttaagcgactccattaagaacgtattttggatggatctagggttttatgccttaggatttttgttttgtagtttttcatccaataagaaattaatcataccaaaaaaggacgagacaccagaagataatttagaagacaagtaacgcataacttcgtataatgtacattatacgaagttatagttattttcttattttcattttatttttttcgccttttatacagacttcaacacaatcagaat BBa_K277010_sequence 1 actcacgataacctgggcaccggtgttcctgattttatctaccattctcttctttgtcgctgtaggaaccacgactgtacatggtagagacagtctttgacatgctgttgcagcagcaaaaccggcattaccgccagaactagcgaaaacctgaggagatcttttaccgtccttttgaattcgaatggcacttttcatgatgagattaccgattcctctacttttgaaggagccacttggttgaaggcattcatatttcaagaaaaattgtgcagaagcctttccggggaagaattgacgtaataatggtgttttattgtagactatcgacatctcgctggttaattttcctgtctcttgtctatccagcacttaaaaaagagaaaactgtataaacattttccttttatttggcgatatttatatacctgtgttcctcctcatattgtcccttttatcttatctcatcgcaaacaatttgataaagctttggcactatttaatgacactcagtggaattacatttccgctggggcttaacaggagccgcccatgcggaatcatgtctccgcgcagaggactcattagtttccgttcgcggtgattagtactcgcgaaaagagaaaaagaaaaatagtaggaacatttcgcaattgatcaaaatgtaaacagtgcagtaaagaatgggaccataagatagataagaaacagctcgcataaggaaacaaggacacatcgattaatggcaacccaagccctagaggatatcacggagaggcttttaataaggtcgcaagaggctatcttgcaattagatctgtggatacaacgt BBa_K277002_sequence 1 aaggttcttgtagagttggttaggcatggtttgtgtccatatggcaccagaaacggaggcaccgatagcagcgcctagctttgaaaaggtatactggatgccggttattacagccatcctactatgcgtagtcatggcttgcagtatgacgatcactgaattgctgcataggagaccactcaaacccatgataacagatgcagcgataacaccttcatgagacccagatcctcctctgtatttgtaaaaaaggcccatgcacaccatccatgcagcacaacctccgattacagaaagtttcagtttccttgtctttgccaccaacaaactgtagaatggagatgcagtagcagcaacaaagtcaggaaggtttactatccgagcagccgaagtggacgattctttcatcgataccagcaaaacaggatacagatagtcacacgaaatgaaaaaggtgaaaaaattgaaaaaagtaacaccaaggggtgcccaaattccacgatcacttagtaatttgaacggtagaagaggagatttggcaaatttggcctcccaatataaaaaaatgaaaaataaacagccacctgaaactaaagtggcaattatttttgaattgtgccacttctgtgatgtctcattagccaacgtcaaagggacaaggatacaccctagcgacacagttattaataatatgccaacaatatcgagtttccagaatagaaacaccaaat BBa_K277001_sequence 1 ataacttcgtataatgtacattatacgaagttattctttctttttcggtacaacataattaatgcattcatacagagcgcatttccagcatcatttattggatagtaactcctcccgcactttcaatatcaatgacgctgactcttttactgacgctgagtctttaccgacgctgagtctttaccgacgctgagtctttaccgacgctgagtctttaccgacgcttgtgaaaataaaaagtacgacacagctatatgcgtcgcaagttcagatgtgggtctatcgagcatgtttatcctatcctgttatgtcatttttatcggaagtaataaaagtgtagaaatcacttgtttttgccttttttgtgattttgctttcatttttttttgcccttttttcaccagcttggggatcaacatcctaatttcttgttacttcgattatgtctaaaaagtgccttgatttgagagaaaatattttcctcatctggcaagacaaccaaaccatcttcagtgaattcttcgtgtgtcgccttgtctattagttccgggtctctcatgaacattgtaaacgcaaagaacggtaccgtacatgccaagccaaccgtcattattattcgttgaacatatctgtaagattcaaccacagcatctctttcgggtgagccccaaggataatcactaatgaatgtataaggtgatgcatatgctatttctgccaatgtatcgtttccaaggttcttgt BBa_K277012_sequence 1 atcagatacacatacggtaaaagagaacaacacaaatcttaagcatgcatcttttatggataagtttaattcatcgttaagcacaatatcagaatcttttcaaagtaagagggggagaaagaataagggcatgaatgaagaacgaatatcaaatcataatgtagcacaggaacaaaaaaataatatggatataagcgtctctatagaagaattgcaagatgctttgaatacagaactgctgttttaattaataacttcgtataatgtacattatacgaagttatatgcctcctttacctaacctactcttcttttgctccaaatgtttattctttttttttttattcagaatatcttcaagtttttttgtatctttttgtattattattaaacttctttataatatttgtcttataaaatcctatcataacatgactatggcttggcctagactcgggtgccatcttttttaatgcgactacttcaagacagcttctg BBa_K277008_sequence 1 aatcagaatcaaataggtgtatcgcaatggaatgtaatttcttaagtattctatatgtacttaaaacctattaatatatggatcaacacagtatcttatgaatgggtttttgatttttttatgtttttttaaaacattaaagttttcggcacggacttatttggaattcaaattattaatgaaagaacaattaactaattaatgtacttagtatttggccattattatcgatttcgggggccaaatctaaccaaattcaacctacattttttcaaattgattcaaacacctttcacaataagatttttatatctagcgcacatagaatgaaatgtaaacaaagatttcagaaaaatcgtataacttcgtataatgtacattatacgaagttatgaaattataagttaaaaagcagtagtttatgctttatgctcgagtatcaagtgaatttgaacaggctagtgcttcattggtacttctttcatggataattttgagcaaatttctgcagcatgtccccctttatacaaattctgtgcattgccggcctagaaatatgtcaacgttttggatatgttgatgcttacttcgagaaatcttacactaatacttctggaaaaaatcaatactagcaaaatagtgatatatgagtaaaatgtatgtagtacatgtatgaaaattatcaagggcaaattgatgcttcaacgaaaaagttattggattttcaagcactttttaaattcacaat BBa_K277011_sequence 1 atacaacgtcagcagagatcatcaatatgccagacaacagatcaagagtcattggataagttatcccaacagtacaaccagtatatgtctcaactgaactctttgtatgttagatcggaatctgttcgagacaagttgagcaaggaacaacaacgcagattgatcacagaggataatgagcatcaacgcatagaagacttggttcgtgaattccaggatatcactttgaggttgaacgagctggccaccgtcccaaatgaagcgcctaatgattctccacaatcgcaatccaccagaagtagtttagggtcatttcaacctcgaccattgaaaataattgagaggcaacgtctgtgtatggtaactccatcgaaaccaccaaaaaaatcggtaggctttaaccccatcaatgaagtcgattgtccttcgaaaactaactctttaccgtgctcacctaaaaaacaacctgcaagaaatcgcactttacgtgcagccaaatcacatgatactggcttgaacaaaagtaagaaaccaagcagcagcgacacctacgaaagctttttcaaaaatagacaaagactttcgttgaccttctttgatgaaatggatgatgaagattttgattctgatcaagatactatcattctaccaaacat BBa_K277004_sequence 1 attacttgaggcaccaacaacactagaactcatccgataatttatttacttgattattccttttttttttttttctctttccagtccataaaactattttacttttcttatttatgtaaatagtatttaagattctacggtcgctacatctgctagaaatggaattactttaatacattccatgttctcatatatacttttactgacacctttcgtcactattttttgtttaactttttttttggaagtactaaaattatatgctcgtaattattgagtatcaaaaaaaaaaaaaaaaaaaattgctgtgacaccccttcaatgtggtgtctatacacctggtgactttaactttaactttgacctgtcgtcgtacaatcaacttagtttccgaaaatccacgacgaaccagttataccgaagacttttgtacgacaagaatactgtaccgtcgaaactatctcttttcaaactgatgagtgaagcttgtgtttatgtctgttgaaacaaggaaaaaaatgcggtgcctgtcgacaggatatgcgcacaagtgcaaaatcatcaaaaaattgagtacctagatccggtggtgtaggacgagcaaagttccttcataaacaaaccgtaagggttactgatacacaatttcctttttgtaaagagtatttgagcaaattagtcagcgattgaaaacaacacccttttttctatatatagtaggtgaaagttaacatgcgagtaaaagtagtgccacatt BBa_K277009_sequence 1 attcacaatatttttttctggaaatatgaaattgtcagcgacttctattacaggagtgtctttttttctcatgctatccaacgcttcttccaaatctttgatggtgttgctgctaccaccgcaagcaataattatcacaatgtcatccgcagctaattttgagcccagtgcattttctaggatcttagtgttgtaacccaaatgcaatgcggcgccacatgccggttcaatcaccatattgaattgatgtgtatatttaagacaggtttcaataacatctttgtcctctattacaacggatctggtgttgtatttgcgagcgtattcgaaagtttggttgctaataacagcggtacccaagctggtagcaatacttgttatcttcttgaattgaactggttggcctattttcaaagaagtattgaaaacatgacatccattcgtttccacccccacaatagggatcctatcagctaaaccatacctttccaaaccttgaataataccattgtataaaccacctccaccaacgctgcatactatgcctttaaccttattcacggaaatatgttgcgatttcaaatcttgtactatttcatctatcatagatgaatgtccttcccaaatatccggattatcgaagggatgaacataaatgggctcaatgacctgagagtctattttattcatgacatttgtttttaaaaaagtatctgcttctttccagtaggcaccactcacgat BBa_K277005_sequence 1 agtgccacatttcctttgcagcaggtataacttcgtataatgtacattatacgaagttataaattggtacatatttaacagatagcgccgcacctcatatgcagcgcgtacatgatacgagacgacaagatatgcaaaagataatagtgtcatcacacctttatgagaagcgaatttttttttttttttggtttaaatatatatatatacatatatatagatatagatatacacatgtatagatgtattctagttatgcttatacctagaactgatcagaccagatcatacctggtatagagcttaatcgatagaaattcaaaaatatggaggaaactaagtactcttcgcagcaggagatagaagaagcatgtggttcagacgcttcattgaatgctagaggtagcaatgattctccaatgggactttccttgtacctctgcctggcttcgtgaactcttgtactattcataactgcactggatattttgatagtgggaactattattgacgtggtcgcagaacagttcggaaactactccaaaacaggttggctcgttacaggctacagtttaccaaatgctattctgagtctcatttggggaagattcgcatctatcataggtttccagcatagtctcattttagcaatacttatttttgaagccggatccctaattgctgcccttgcctcttcaatgaatatgctcattgtcggtagagttgttgctagtgttgggggaagcggact BBa_K277006_sequence 1 aagcggacttcaaacgctttgctttgttattggttgtacgatggttggtgaaaggtcacgtccattggtgatttccatcctaagttgtgcatttgctgtagctgctatcgttggtcctataatcggaggtgcctttacaacccatgttacctggaggtggtgcttctatatcaatcttcctatcggtggtcttgccattattatgtttttactcacatataaggccgagaataagggtatacttcaacaaattaaagatgctataggaacaatctcgagctttacttttagtaagttcagacaccaagttaattttaaaagacttatgaatggcataatcttcaagtttgacttctttggttttgccctctgctctgcagggctggtccttttcctactggggctaacctttggtggtaataaatatagttggaactctggccaagtcatcgcatatttggttttgggtgtcttactttttattttttcattggtgtacgatttcttcttattcgataaattcaacccggaacctgataatatatcctacaggcctctccttctaagaagattggtagcaaaaccagccataataataataaacatggtaacatttctattatgtaccggttacaatgggcaaatgatatactctgtccagtttttccaacttatatttgcgtcgagtgcatggaaagccggtcttcacttgataccaatcgttattaccaacgttattgcggccattgcaagt BBa_K277003_sequence 1 acaccaaattctcaaataatccgcccgttctttcctttctagcctgttctttgagagatctccactcagcagtctttgaagatttgtacttcatataaagaataagaaatataattggcaaggcagagagtgggtaaataaaagcccacattgcaatattccaggaccagtttttctgaggatttgctgctgtgataatattacctgaaatccatggtattatgatatatggccaatatgaggcgtactggtaaaacattctccacttcaaggaggagaaatcagaaagtattaatgtcaggagcagatttgttccgacgtatccacagttatagaaaaccgatcctgctgcatacattgtgagacgggtcgcctgtgattgaatgatggttcccattatataaaaaatagttgcaactaaaaaaagccttagtcttccgaagtggtcagagagtctggagtagacaacttgggatccgacacttacaacagcattgataacttggacagttgaaagtaaggagtgttctgaatatgagttcgtcgcatagcccgtataggtcgatctaagtgtgtagtctaaactaattccaaacccacatacaaacgcggtacttatcagtagaattttatatttcaaggaatcaaactgtgcagacataatttcgttttctttgagcttaaaaaaggtcgatgtcatagagtacgtgtcatcgtttgagtgacgttctcgctcaataatctcacaggactgcctaattccactttttttattactt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z