BBa_K277134
type: ComponentDefinition
persistent identity: BBa K277134
display id: BBa_K277134
version: 1
was generated by: iGEM to SBOL conversion
description: 3L.3_23.A1
created: 2009-10-20T11:00:00Z
modified: 2015-05-08T01:11:47Z
mutable provenance: Synthetic Yeast
owned by: james
owned by: myers
mutable description: 3L.3_23.A1 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns,repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.A1 is comprised of and must be constructed from the parts 3L.3_23.A1.01, 3L.3_23.A1.02, 3L.3_23.A1.03, 3L.3_23.A1.04, 3L.3_23.A1.05, 3L.3_23.A1.06, 3L.3_23.A1.07, 3L.3_23.A1.08, 3L.3_23.A1.09, 3L.3_23.A1.10, 3L.3_23.A1.11, and 3L.3_23.A1.12. This part wholly contains the following features (positions offset from first base of sequence): kind and name offset notes loxP_site loxpsym_delyeast103_25(chrIII)3000..3001 (3000..3033) gene YCL073C (413..2260) Protein of unconfirmed function%3B displays a topology characteristic of the Major Facilitators Superfamily of membrane proteins%3B coding sequence 98% identical to that of YKR106W loxP_site loxPsym_YCL063W (8632..8665) gene YCL069W (3672..5048) Permease of basic amino acids in the vacuolar membrane ARS ARS320 (5534..6341) Autonomously Replicating Sequence on Chromosome III loxP_site loxpsym_delyeast_chr3_3_17(chrIII)5500..9500 (5500..5533) reverse_primer YCL063W_tagr1v1 (8223..8250) loxP_site loxpsym_delyeast103_24(chrIII)1..6100 (1..34) mutation_affecting_coding_sequence YCL069W_re_remove_AlwNI (4680..4691) removal of AlwNI forward_primer YCL064C_tagf1v1 (5973..6000) loxP_site loxPsym_YCL069W (5052..5085) gene YCL063W (7357..8628) Protein involved in vacuole inheritance%3B acts as a vacuole-specific receptor for myosin Myo2p forward_primer YCL063W_tagf1v1 (7971..7998) reverse_primer YCL064C_tagr1v1 (6216..6243) ARS ARS301 (5146..5401)…
discontinued: false
dominant: false
group u list: _385_
m user id: 0
owner id: 2669
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Constructed using USER assembly method described in RFC38
star: false
creator: James DiCarlo
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K277001
component: BBa_K277003
component: BBa_K277002
component: BBa_K277005
component: BBa_K277004
component: BBa_K277007
component: BBa_K277006
component: BBa_K277009
component: BBa_K277008
component: BBa_K277011
component: BBa_K277010
component: BBa_K277012
sequence annotation: BBa_K277001
sequence annotation: BBa_K277002
sequence annotation: BBa_K277003
sequence annotation: BBa_K277004
sequence annotation: BBa_K277005
sequence annotation: BBa_K277006
sequence annotation: BBa_K277007
sequence annotation: BBa_K277008
sequence annotation: BBa_K277009
sequence annotation: BBa_K277010
sequence annotation: BBa_K277011
sequence annotation: BBa_K277012
sequence: https://synbiohub.org/public/igem/BBa_K277134_sequence/1

BBa_K277001
type: Component
persistent identity: component2061979
display id: component2061979
version: 1
top level: BBa_K277134
definition: BBa_K277001
access: public

BBa_K277003
type: Component
persistent identity: component2061981
display id: component2061981
version: 1
top level: BBa_K277134
definition: BBa_K277003
access: public

BBa_K277002
type: Component
persistent identity: component2061980
display id: component2061980
version: 1
top level: BBa_K277134
definition: BBa_K277002
access: public

BBa_K277005
type: Component
persistent identity: component2061983
display id: component2061983
version: 1
top level: BBa_K277134
definition: BBa_K277005
access: public

BBa_K277004
type: Component
persistent identity: component2061982
display id: component2061982
version: 1
top level: BBa_K277134
definition: BBa_K277004
access: public

BBa_K277007
type: Component
persistent identity: component2061985
display id: component2061985
version: 1
top level: BBa_K277134
definition: BBa_K277007
access: public

BBa_K277006
type: Component
persistent identity: component2061984
display id: component2061984
version: 1
top level: BBa_K277134
definition: BBa_K277006
access: public

BBa_K277009
type: Component
persistent identity: component2061987
display id: component2061987
version: 1
top level: BBa_K277134
definition: BBa_K277009
access: public

BBa_K277008
type: Component
persistent identity: component2061986
display id: component2061986
version: 1
top level: BBa_K277134
definition: BBa_K277008
access: public

BBa_K277011
type: Component
persistent identity: component2061989
display id: component2061989
version: 1
top level: BBa_K277134
definition: BBa_K277011
access: public

BBa_K277010
type: Component
persistent identity: component2061988
display id: component2061988
version: 1
top level: BBa_K277134
definition: BBa_K277010
access: public

BBa_K277012
type: Component
persistent identity: component2061990
display id: component2061990
version: 1
top level: BBa_K277134
definition: BBa_K277012
access: public

BBa_K277001
type: SequenceAnnotation
persistent identity: annotation2061979
display id: annotation2061979
version: 1
top level: BBa_K277134
location: https://synbiohub.org/public/igem/BBa_K277134/annotation2061979/range2061979/1
component: BBa_K277001

https://synbiohub.org/public/igem/BBa_K277134/annotation2061979/range2061979/1
type: Range
persistent identity: range2061979
display id: range2061979
version: 1
top level: BBa_K277134
direction: direction/reverse
start: 1
end: 744
orientation: inline

BBa_K277002
type: SequenceAnnotation
persistent identity: annotation2061980
display id: annotation2061980
version: 1
top level: BBa_K277134
location: https://synbiohub.org/public/igem/BBa_K277134/annotation2061980/range2061980/1
component: BBa_K277002

https://synbiohub.org/public/igem/BBa_K277134/annotation2061980/range2061980/1
type: Range
persistent identity: range2061980
display id: range2061980
version: 1
top level: BBa_K277134
direction: direction/reverse
start: 753
end: 1483
orientation: inline

BBa_K277003
type: SequenceAnnotation
persistent identity: annotation2061981
display id: annotation2061981
version: 1
top level: BBa_K277134
location: https://synbiohub.org/public/igem/BBa_K277134/annotation2061981/range2061981/1
component: BBa_K277003

https://synbiohub.org/public/igem/BBa_K277134/annotation2061981/range2061981/1
type: Range
persistent identity: range2061981
display id: range2061981
version: 1
top level: BBa_K277134
direction: direction/reverse
start: 1492
end: 2270
orientation: inline

BBa_K277004
type: SequenceAnnotation
persistent identity: annotation2061982
display id: annotation2061982
version: 1
top level: BBa_K277134
location: https://synbiohub.org/public/igem/BBa_K277134/annotation2061982/range2061982/1
component: BBa_K277004

https://synbiohub.org/public/igem/BBa_K277134/annotation2061982/range2061982/1
type: Range
persistent identity: range2061982
display id: range2061982
version: 1
top level: BBa_K277134
direction: direction/reverse
start: 2279
end: 3035
orientation: inline

BBa_K277005
type: SequenceAnnotation
persistent identity: annotation2061983
display id: annotation2061983
version: 1
top level: BBa_K277134
location: https://synbiohub.org/public/igem/BBa_K277134/annotation2061983/range2061983/1
component: BBa_K277005

https://synbiohub.org/public/igem/BBa_K277134/annotation2061983/range2061983/1
type: Range
persistent identity: range2061983
display id: range2061983
version: 1
top level: BBa_K277134
direction: direction/reverse
start: 3044
end: 3794
orientation: inline

BBa_K277006
type: SequenceAnnotation
persistent identity: annotation2061984
display id: annotation2061984
version: 1
top level: BBa_K277134
location: https://synbiohub.org/public/igem/BBa_K277134/annotation2061984/range2061984/1
component: BBa_K277006

https://synbiohub.org/public/igem/BBa_K277134/annotation2061984/range2061984/1
type: Range
persistent identity: range2061984
display id: range2061984
version: 1
top level: BBa_K277134
direction: direction/reverse
start: 3803
end: 4556
orientation: inline

BBa_K277007
type: SequenceAnnotation
persistent identity: annotation2061985
display id: annotation2061985
version: 1
top level: BBa_K277134
location: https://synbiohub.org/public/igem/BBa_K277134/annotation2061985/range2061985/1
component: BBa_K277007

https://synbiohub.org/public/igem/BBa_K277134/annotation2061985/range2061985/1
type: Range
persistent identity: range2061985
display id: range2061985
version: 1
top level: BBa_K277134
direction: direction/reverse
start: 4565
end: 5253
orientation: inline

BBa_K277008
type: SequenceAnnotation
persistent identity: annotation2061986
display id: annotation2061986
version: 1
top level: BBa_K277134
location: https://synbiohub.org/public/igem/BBa_K277134/annotation2061986/range2061986/1
component: BBa_K277008

https://synbiohub.org/public/igem/BBa_K277134/annotation2061986/range2061986/1
type: Range
persistent identity: range2061986
display id: range2061986
version: 1
top level: BBa_K277134
direction: direction/reverse
start: 5262
end: 6009
orientation: inline

BBa_K277009
type: SequenceAnnotation
persistent identity: annotation2061987
display id: annotation2061987
version: 1
top level: BBa_K277134
location: https://synbiohub.org/public/igem/BBa_K277134/annotation2061987/range2061987/1
component: BBa_K277009

https://synbiohub.org/public/igem/BBa_K277134/annotation2061987/range2061987/1
type: Range
persistent identity: range2061987
display id: range2061987
version: 1
top level: BBa_K277134
direction: direction/reverse
start: 6018
end: 6761
orientation: inline

BBa_K277010
type: SequenceAnnotation
persistent identity: annotation2061988
display id: annotation2061988
version: 1
top level: BBa_K277134
location: https://synbiohub.org/public/igem/BBa_K277134/annotation2061988/range2061988/1
component: BBa_K277010

https://synbiohub.org/public/igem/BBa_K277134/annotation2061988/range2061988/1
type: Range
persistent identity: range2061988
display id: range2061988
version: 1
top level: BBa_K277134
direction: direction/reverse
start: 6770
end: 7601
orientation: inline

BBa_K277011
type: SequenceAnnotation
persistent identity: annotation2061989
display id: annotation2061989
version: 1
top level: BBa_K277134
location: https://synbiohub.org/public/igem/BBa_K277134/annotation2061989/range2061989/1
component: BBa_K277011

https://synbiohub.org/public/igem/BBa_K277134/annotation2061989/range2061989/1
type: Range
persistent identity: range2061989
display id: range2061989
version: 1
top level: BBa_K277134
direction: direction/reverse
start: 7610
end: 8265
orientation: inline

BBa_K277012
type: SequenceAnnotation
persistent identity: annotation2061990
display id: annotation2061990
version: 1
top level: BBa_K277134
location: https://synbiohub.org/public/igem/BBa_K277134/annotation2061990/range2061990/1
component: BBa_K277012

https://synbiohub.org/public/igem/BBa_K277134/annotation2061990/range2061990/1
type: Range
persistent identity: range2061990
display id: range2061990
version: 1
top level: BBa_K277134
direction: direction/reverse
start: 8274
end: 8771
orientation: inline

BBa_K277001
type: ComponentDefinition
persistent identity: BBa K277001
display id: BBa_K277001
version: 1
was generated by: iGEM to SBOL conversion
description: 3L.3_23.A1.01
created: 2009-10-19T11:00:00Z
modified: 2015-05-08T01:11:45Z
mutable provenance: Synthetic Yeast
owned by: james
owned by: myers
mutable description: 3L.3_23.A1.01 is 744 bases long and is cloned into the pGem-T vector. 3L.3_23.A1.01 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.A1.01 is a constituent of 3L.3_23.A1 (along with 3L.3_23.A1.02, 3L.3_23.A1.03, 3L.3_23.A1.04, 3L.3_23.A1.05, 3L.3_23.A1.06, 3L.3_23.A1.07, 3L.3_23.A1.08, 3L.3_23.A1.09, 3L.3_23.A1.10, 3L.3_23.A1.11, and 3L.3_23.A1.12.) This part contains at least part of the following features (positions offset from first base of sequence): kind and name offset notes gene YCL073C (413..+2260) Protein of unconfirmed function%3B displays a topology characteristic of the Major Facilitators Superfamily of membrane proteins%3B coding sequence 98% identical to that of YKR106W loxP_site loxpsym_delyeast103_24(chrIII)1..6100 (1..34) Sequence (the first 744 bases correspond to coordinates 1..744 in synthetic chromosome yeast_chr3_3_23)
discontinued: false
dominant: false
group u list: _385_
m user id: 0
owner id: 2669
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Built with overlap extension PCR
star: false
creator: James DiCarlo
type: DnaRegion
role: SO:0000110
role: partType/Other
sequence: https://synbiohub.org/public/igem/BBa_K277001_sequence/1

BBa_K277002
type: ComponentDefinition
persistent identity: BBa K277002
display id: BBa_K277002
version: 1
was generated by: iGEM to SBOL conversion
description: 3L.3_23.A1.02
created: 2009-10-19T11:00:00Z
modified: 2015-05-08T01:11:45Z
mutable provenance: Synthetic Yeast
owned by: james
owned by: myers
mutable description: 3L.3_23.A1.02 is 731 bases long and is cloned into the pGem-T vector. 3L.3_23.A1.02 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.A1.02 is a constituent of 3L.3_23.A1 (along with 3L.3_23.A1.01, 3L.3_23.A1.03, 3L.3_23.A1.04, 3L.3_23.A1.05, 3L.3_23.A1.06, 3L.3_23.A1.07, 3L.3_23.A1.08, 3L.3_23.A1.09, 3L.3_23.A1.10, 3L.3_23.A1.11, and 3L.3_23.A1.12.) This part contains at least part of the following features (positions offset from first base of sequence): kind and name offset notes gene YCL073C (-320..+1527) Protein of unconfirmed function%3B displays a topology characteristic of the Major Facilitators Superfamily of membrane proteins%3B coding sequence 98% identical to that of YKR106W Sequence (the first 731 bases correspond to coordinates 734..1464 in synthetic chromosome yeast_chr3_3_23)
discontinued: false
dominant: false
group u list: _385_
m user id: 0
owner id: 2669
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Assembled by Overlap extension PCR
star: false
creator: James DiCarlo
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K277002_sequence/1

BBa_K277003
type: ComponentDefinition
persistent identity: BBa K277003
display id: BBa_K277003
version: 1
was generated by: iGEM to SBOL conversion
description: 3L.3_23.A1.03
created: 2009-10-19T11:00:00Z
modified: 2015-05-08T01:11:45Z
mutable provenance: synthetic Yeast
owned by: james
owned by: myers
mutable description: 3L.3_23.A1.03 is 779 bases long and is cloned into the pGem-T vector. 3L.3_23.A1.03 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.A1.03 is a constituent of 3L.3_23.A1 (along with 3L.3_23.A1.01, 3L.3_23.A1.02, 3L.3_23.A1.04, 3L.3_23.A1.05, 3L.3_23.A1.06, 3L.3_23.A1.07, 3L.3_23.A1.08, 3L.3_23.A1.09, 3L.3_23.A1.10, 3L.3_23.A1.11, and 3L.3_23.A1.12.) This part contains at least part of the following features (positions offset from first base of sequence): kind and name offset notes gene YCL073C (-1042..+805) Protein of unconfirmed function%3B displays a topology characteristic of the Major Facilitators Superfamily of membrane proteins%3B coding sequence 98% identical to that of YKR106W Sequence (the first 779 bases correspond to coordinates 1456..2234 in synthetic chromosome yeast_chr3_3_23)
discontinued: false
dominant: false
group u list: _385_
m user id: 0
owner id: 2669
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Constructed using overlap extension PCR proto descibed in RFC38
star: false
creator: James DiCarlo
type: DnaRegion
role: SO:0000110
role: partType/Other
sequence: https://synbiohub.org/public/igem/BBa_K277003_sequence/1

BBa_K277004
type: ComponentDefinition
persistent identity: BBa K277004
display id: BBa_K277004
version: 1
was generated by: iGEM to SBOL conversion
description: 3L.3_23.A1.04
created: 2009-10-19T11:00:00Z
modified: 2015-05-08T01:11:45Z
mutable provenance: synthetic Yeast
owned by: james
owned by: myers
mutable description: 3L.3_23.A1.04 is 757 bases long and is cloned into the pGem-T vector. 3L.3_23.A1.04 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.A1.04 is a constituent of 3L.3_23.A1 (along with 3L.3_23.A1.01, 3L.3_23.A1.02, 3L.3_23.A1.03, 3L.3_23.A1.05, 3L.3_23.A1.06, 3L.3_23.A1.07, 3L.3_23.A1.08, 3L.3_23.A1.09, 3L.3_23.A1.10, 3L.3_23.A1.11, and 3L.3_23.A1.12.) This part contains at least part of the following features (positions offset from first base of sequence): kind and name offset notes gene YCL073C (-1814..33) Protein of unconfirmed function%3B displays a topology characteristic of the Major Facilitators Superfamily of membrane proteins%3B coding sequence 98% identical to that of YKR106W Sequence (the first 757 bases correspond to coordinates 2228..2984 in synthetic chromosome yeast_chr3_3_23)
discontinued: false
dominant: false
group u list: _385_
m user id: 0
owner id: 2669
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Constructed using overlap extension PCR protocol described in RFC38
star: false
creator: James DiCarlo
type: DnaRegion
role: SO:0000110
role: partType/Other
sequence: https://synbiohub.org/public/igem/BBa_K277004_sequence/1

BBa_K277005
type: ComponentDefinition
persistent identity: BBa K277005
display id: BBa_K277005
version: 1
was generated by: iGEM to SBOL conversion
description: 3L.3_23.A1.05
created: 2009-10-19T11:00:00Z
modified: 2015-05-08T01:11:45Z
mutable provenance: synthetic Yeast
owned by: james
owned by: myers
mutable description: 3L.3_23.A1.05 is 751 bases long and is cloned into the pGem-T vector. 3L.3_23.A1.05 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.A1.05 is a constituent of 3L.3_23.A1 (along with 3L.3_23.A1.01, 3L.3_23.A1.02, 3L.3_23.A1.03, 3L.3_23.A1.04, 3L.3_23.A1.06, 3L.3_23.A1.07, 3L.3_23.A1.08, 3L.3_23.A1.09, 3L.3_23.A1.10, 3L.3_23.A1.11, and 3L.3_23.A1.12.) This part contains at least part of the following features (positions offset from first base of sequence): kind and name offset notes loxP_site loxpsym_delyeast103_25(chrIII)3000..3001 (27..60) gene YCL069W (699..+2075) Permease of basic amino acids in the vacuolar membrane Sequence (the first 751 bases correspond to coordinates 2974..3724 in synthetic chromosome yeast_chr3_3_23)
discontinued: false
dominant: false
group u list: _385_
m user id: 0
owner id: 2669
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Constructed using overlap extension PCR protocol described in RFC38
star: false
creator: James DiCarlo
type: DnaRegion
role: SO:0000110
role: partType/Other
sequence: https://synbiohub.org/public/igem/BBa_K277005_sequence/1

BBa_K277006
type: ComponentDefinition
persistent identity: BBa K277006
display id: BBa_K277006
version: 1
was generated by: iGEM to SBOL conversion
description: 3L.3_23.A1.06
created: 2009-10-19T11:00:00Z
modified: 2015-05-08T01:11:45Z
mutable provenance: synthetic Yeast
owned by: james
owned by: myers
mutable description: 3L.3_23.A1.06 is 754 bases long and is cloned into the pGem-T vector. 3L.3_23.A1.06 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.A1.06 is a constituent of 3L.3_23.A1 (along with 3L.3_23.A1.01, 3L.3_23.A1.02, 3L.3_23.A1.03, 3L.3_23.A1.04, 3L.3_23.A1.05, 3L.3_23.A1.07, 3L.3_23.A1.08, 3L.3_23.A1.09, 3L.3_23.A1.10, 3L.3_23.A1.11, and 3L.3_23.A1.12.) This part contains at least part of the following features (positions offset from first base of sequence): kind and name offset notes gene YCL069W (-43..+1333) Permease of basic amino acids in the vacuolar membrane Sequence (the first 754 bases correspond to coordinates 3716..4469 in synthetic chromosome yeast_chr3_3_23):
discontinued: false
dominant: false
group u list: _385_
m user id: 0
owner id: 2669
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Constructed using overlap extension PCR protocol described in RFC38
star: false
creator: James DiCarlo
type: DnaRegion
role: SO:0000110
role: partType/Other
sequence: https://synbiohub.org/public/igem/BBa_K277006_sequence/1

BBa_K277007
type: ComponentDefinition
persistent identity: BBa K277007
display id: BBa_K277007
version: 1
was generated by: iGEM to SBOL conversion
description: 3L.3_23.A1.07
created: 2009-10-19T11:00:00Z
modified: 2015-05-08T01:11:45Z
mutable provenance: synthetic Yeast
owned by: james
owned by: myers
mutable description: 3L.3_23.A1.07 is 689 bases long and is cloned into the pGem-T vector. 3L.3_23.A1.07 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.A1.07 is a constituent of 3L.3_23.A1 (along with 3L.3_23.A1.01, 3L.3_23.A1.02, 3L.3_23.A1.03, 3L.3_23.A1.04, 3L.3_23.A1.05, 3L.3_23.A1.06, 3L.3_23.A1.08, 3L.3_23.A1.09, 3L.3_23.A1.10, 3L.3_23.A1.11, and 3L.3_23.A1.12.) This part contains at least part of the following features (positions offset from first base of sequence): kind and name offset notes gene YCL069W (-788..588) Permease of basic amino acids in the vacuolar membrane mutation_affecting_coding_sequence YCL069W_re_remove_AlwNI (220..231) removal of AlwNI loxP_site loxPsym_YCL069W (592..625) ARS ARS301 (686..+941) Inactive replication origin associated with the silent mating type locus HML%2C where it functions as a transcriptional silencer Sequence (the first 689 bases correspond to coordinates 4461..5149 in synthetic chromosome yeast_chr3_3_23)
discontinued: false
dominant: false
group u list: _385_
m user id: 0
owner id: 2669
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Constructed using overlap extension PCR protocol described in RFC38
star: false
creator: James DiCarlo
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K277007_sequence/1

BBa_K277008
type: ComponentDefinition
persistent identity: BBa K277008
display id: BBa_K277008
version: 1
was generated by: iGEM to SBOL conversion
description: 3L.3_23.A1.08
created: 2009-10-19T11:00:00Z
modified: 2015-05-08T01:11:45Z
mutable provenance: synthetic Yeast
owned by: james
owned by: myers
mutable description: 3L.3_23.A1.08 is 748 bases long and is cloned into the pGem-T vector. 3L.3_23.A1.08 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.A1.08 is a constituent of 3L.3_23.A1 (along with 3L.3_23.A1.01, 3L.3_23.A1.02, 3L.3_23.A1.03, 3L.3_23.A1.04, 3L.3_23.A1.05, 3L.3_23.A1.06, 3L.3_23.A1.07, 3L.3_23.A1.09, 3L.3_23.A1.10, 3L.3_23.A1.11, and 3L.3_23.A1.12.) This part contains at least part of the following features (positions offset from first base of sequence): kind and name offset notes ARS ARS320 (394..+1201) Autonomously Replicating Sequence on Chromosome III loxP_site loxpsym_delyeast_chr3_3_17(chrIII)5500..9500 (360..393) ARS ARS301 (6..261) Inactive replication origin associated with the silent mating type locus HML%2C where it functions as a transcriptional silencer gene YCL064C (725..+1807) Catabolic L-serine (L-threonine) deaminase%2C catalyzes the degradation of both L-serine and L-threonine%3B required to use serine or threonine as the sole nitrogen source%2C transcriptionally induced by serine and threonine Sequence (the first 748 bases correspond to coordinates 5141..5888 in synthetic chromosome yeast_chr3_3_23)
discontinued: false
dominant: false
group u list: _385_
m user id: 0
owner id: 2669
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Constructed using overlap extension PCR protocol described in RFC38
star: false
creator: James DiCarlo
type: DnaRegion
role: SO:0000110
role: partType/Other
sequence: https://synbiohub.org/public/igem/BBa_K277008_sequence/1

BBa_K277009
type: ComponentDefinition
persistent identity: BBa K277009
display id: BBa_K277009
version: 1
was generated by: iGEM to SBOL conversion
description: 3L.3_23.A1.09
created: 2009-10-19T11:00:00Z
modified: 2015-05-08T01:11:45Z
mutable provenance: Synthetic Yeast
owned by: james
owned by: myers
mutable description: 3L.3_23.A1.09 is 744 bases long and is cloned into the pGem-T vector. 3L.3_23.A1.09 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.A1.09 is a constituent of 3L.3_23.A1 (along with 3L.3_23.A1.01, 3L.3_23.A1.02, 3L.3_23.A1.03, 3L.3_23.A1.04, 3L.3_23.A1.05, 3L.3_23.A1.06, 3L.3_23.A1.07, 3L.3_23.A1.08, 3L.3_23.A1.10, 3L.3_23.A1.11, and 3L.3_23.A1.12.) This part contains at least part of the following features (positions offset from first base of sequence): kind and name offset notes ARS ARS320 (-345..462) Autonomously Replicating Sequence on Chromosome III forward_primer YCL064C_tagf1v1 (94..121) reverse_primer YCL064C_tagr1v1 (337..364) gene YCL064C (-14..+1068) Catabolic L-serine (L-threonine) deaminase%2C catalyzes the degradation of both L-serine and L-threonine%3B required to use serine or threonine as the sole nitrogen source%2C transcriptionally induced by serine and threonine Sequence (the first 744 bases correspond to coordinates 5880..6623 in synthetic chromosome yeast_chr3_3_23)
discontinued: false
dominant: false
group u list: _385_
m user id: 0
owner id: 2669
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Constructed using overlap extension PCR protocol described in RFC38
star: false
creator: James DiCarlo
type: DnaRegion
role: SO:0000110
role: partType/Other
sequence: https://synbiohub.org/public/igem/BBa_K277009_sequence/1

BBa_K277010
type: ComponentDefinition
persistent identity: BBa K277010
display id: BBa_K277010
version: 1
was generated by: iGEM to SBOL conversion
description: 3L.3_23.A1.10
created: 2009-10-19T11:00:00Z
modified: 2015-05-08T01:11:45Z
mutable provenance: Synthetic Yeast
owned by: james
owned by: myers
mutable description: 3L.3_23.A1.10 is 832 bases long and is cloned into the pGem-T vector. 3L.3_23.A1.10 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.A1.10 is a constituent of 3L.3_23.A1 (along with 3L.3_23.A1.01, 3L.3_23.A1.02, 3L.3_23.A1.03, 3L.3_23.A1.04, 3L.3_23.A1.05, 3L.3_23.A1.06, 3L.3_23.A1.07, 3L.3_23.A1.08, 3L.3_23.A1.09, 3L.3_23.A1.11, and 3L.3_23.A1.12.) This part contains at least part of the following features (positions offset from first base of sequence): kind and name offset notes gene YCL063W (743..+2014) Protein involved in vacuole inheritance%3B acts as a vacuole-specific receptor for myosin Myo2p gene YCL064C (-749..333) Catabolic L-serine (L-threonine) deaminase%2C catalyzes the degradation of both L-serine and L-threonine%3B required to use serine or threonine as the sole nitrogen source%2C transcriptionally induced by serine and threonine Sequence (the first 832 bases correspond to coordinates 6615..7446 in synthetic chromosome yeast_chr3_3_23)
discontinued: false
dominant: false
group u list: _385_
m user id: 0
owner id: 2669
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Constructed using overlap extension PCR protocol described in RFC38
star: false
creator: James DiCarlo
type: DnaRegion
role: SO:0000110
role: partType/Other
sequence: https://synbiohub.org/public/igem/BBa_K277010_sequence/1

BBa_K277011
type: ComponentDefinition
persistent identity: BBa K277011
display id: BBa_K277011
version: 1
was generated by: iGEM to SBOL conversion
description: 3L.3_23.A1.11
created: 2009-10-19T11:00:00Z
modified: 2015-05-08T01:11:45Z
mutable provenance: Synthetic Yeast
owned by: james
owned by: myers
mutable description: 3L.3_23.A1.11 is 656 bases long and is cloned into the pGem-T vector. 3L.3_23.A1.11 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.A1.11 is a constituent of 3L.3_23.A1 (along with 3L.3_23.A1.01, 3L.3_23.A1.02, 3L.3_23.A1.03, 3L.3_23.A1.04, 3L.3_23.A1.05, 3L.3_23.A1.06, 3L.3_23.A1.07, 3L.3_23.A1.08, 3L.3_23.A1.09, 3L.3_23.A1.10, and 3L.3_23.A1.12.) This part contains at least part of the following features (positions offset from first base of sequence): kind and name offset notes gene YCL063W (-80..+1191) Protein involved in vacuole inheritance%3B acts as a vacuole-specific receptor for myosin Myo2p forward_primer YCL063W_tagf1v1 (534..561) Sequence (the first 656 bases correspond to coordinates 7438..8093 in synthetic chromosome yeast_chr3_3_23)
discontinued: false
dominant: false
group u list: _385_
m user id: 0
owner id: 2669
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Constructed using overlap extension PCR protocol described in RFC38
star: false
creator: James DiCarlo
type: DnaRegion
role: SO:0000110
role: partType/Other
sequence: https://synbiohub.org/public/igem/BBa_K277011_sequence/1

BBa_K277012
type: ComponentDefinition
persistent identity: BBa K277012
display id: BBa_K277012
version: 1
was generated by: iGEM to SBOL conversion
description: 3L.3_23.A1.12
created: 2009-10-19T11:00:00Z
modified: 2015-05-08T01:11:45Z
mutable provenance: Synthetic Yeast
owned by: james
owned by: myers
mutable description: 3L.3_23.A1.12 is 795 bases long and is cloned into the pGem-T vector. 3L.3_23.A1.12 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.A1.12 is a constituent of 3L.3_23.A1 (along with 3L.3_23.A1.01, 3L.3_23.A1.02, 3L.3_23.A1.03, 3L.3_23.A1.04, 3L.3_23.A1.05, 3L.3_23.A1.06, 3L.3_23.A1.07, 3L.3_23.A1.08, 3L.3_23.A1.09, 3L.3_23.A1.10, and 3L.3_23.A1.11.) This part contains at least part of the following features (positions offset from first base of sequence): kind and name offset notes loxP_site loxPsym_YCL063W (548..581) reverse_primer YCL063W_tagr1v1 (139..166) gene YCL063W (-727..544) Protein involved in vacuole inheritance%3B acts as a vacuole-specific receptor for myosin Myo2p Sequence (the first 795 bases correspond to coordinates 8085..8879 in synthetic chromosome yeast_chr3_3_23)
discontinued: false
dominant: false
group u list: _385_
m user id: 0
owner id: 2669
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Constructed using overlap extension PCR protocol described in RFC38
star: false
creator: James DiCarlo
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K277012_sequence/1

https://synbiohub.org/public/igem/BBa_K277134_sequence/1
type: Sequence
persistent identity: BBa K277134 sequence
display id: BBa_K277134_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ataacttcgtataatgtacattatacgaagttattctttctttttcggtacaacataattaatgcattcatacagagcgcatttccagcatcatttattggatagtaactcctcccgcactttcaatatcaatgacgctgactcttttactgacgctgagtctttaccgacgctgagtctttaccgacgctgagtctttaccgacgctgagtctttaccgacgcttgtgaaaataaaaagtacgacacagctatatgcgtcgcaagttcagatgtgggtctatcgagcatgtttatcctatcctgttatgtcatttttatcggaagtaataaaagtgtagaaatcacttgtttttgccttttttgtgattttgctttcatttttttttgcccttttttcaccagcttggggatcaacatcctaatttcttgttacttcgattatgtctaaaaagtgccttgatttgagagaaaatattttcctcatctggcaagacaaccaaaccatcttcagtgaattcttcgtgtgtcgccttgtctattagttccgggtctctcatgaacattgtaaacgcaaagaacggtaccgtacatgccaagccaaccgtcattattattcgttgaacatatctgtaagattcaaccacagcatctctttcgggtgagccccaaggataatcactaatgaatgtataaggtgatgcatatgctatttctgccaatgtatcgtttccaaggttcttgttactagagaaggttcttgtagagttggttaggcatggtttgtgtccatatggcaccagaaacggaggcaccgatagcagcgcctagctttgaaaaggtatactggatgccggttattacagccatcctactatgcgtagtcatggcttgcagtatgacgatcactgaattgctgcataggagaccactcaaacccatgataacagatgcagcgataacaccttcatgagacccagatcctcctctgtatttgtaaaaaaggcccatgcacaccatccatgcagcacaacctccgattacagaaagtttcagtttccttgtctttgccaccaacaaactgtagaatggagatgcagtagcagcaacaaagtcaggaaggtttactatccgagcagccgaagtggacgattctttcatcgataccagcaaaacaggatacagatagtcacacgaaatgaaaaaggtgaaaaaattgaaaaaagtaacaccaaggggtgcccaaattccacgatcacttagtaatttgaacggtagaagaggagatttggcaaatttggcctcccaatataaaaaaatgaaaaataaacagccacctgaaactaaagtggcaattatttttgaattgtgccacttctgtgatgtctcattagccaacgtcaaagggacaaggatacaccctagcgacacagttattaataatatgccaacaatatcgagtttccagaatagaaacaccaaattactagagacaccaaattctcaaataatccgcccgttctttcctttctagcctgttctttgagagatctccactcagcagtctttgaagatttgtacttcatataaagaataagaaatataattggcaaggcagagagtgggtaaataaaagcccacattgcaatattccaggaccagtttttctgaggatttgctgctgtgataatattacctgaaatccatggtattatgatatatggccaatatgaggcgtactggtaaaacattctccacttcaaggaggagaaatcagaaagtattaatgtcaggagcagatttgttccgacgtatccacagttatagaaaaccgatcctgctgcatacattgtgagacgggtcgcctgtgattgaatgatggttcccattatataaaaaatagttgcaactaaaaaaagccttagtcttccgaagtggtcagagagtctggagtagacaacttgggatccgacacttacaacagcattgataacttggaca…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K277001_sequence/1
type: Sequence
persistent identity: BBa K277001 sequence
display id: BBa_K277001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ataacttcgtataatgtacattatacgaagttattctttctttttcggtacaacataattaatgcattcatacagagcgcatttccagcatcatttattggatagtaactcctcccgcactttcaatatcaatgacgctgactcttttactgacgctgagtctttaccgacgctgagtctttaccgacgctgagtctttaccgacgctgagtctttaccgacgcttgtgaaaataaaaagtacgacacagctatatgcgtcgcaagttcagatgtgggtctatcgagcatgtttatcctatcctgttatgtcatttttatcggaagtaataaaagtgtagaaatcacttgtttttgccttttttgtgattttgctttcatttttttttgcccttttttcaccagcttggggatcaacatcctaatttcttgttacttcgattatgtctaaaaagtgccttgatttgagagaaaatattttcctcatctggcaagacaaccaaaccatcttcagtgaattcttcgtgtgtcgccttgtctattagttccgggtctctcatgaacattgtaaacgcaaagaacggtaccgtacatgccaagccaaccgtcattattattcgttgaacatatctgtaagattcaaccacagcatctctttcgggtgagccccaaggataatcactaatgaatgtataaggtgatgcatatgctatttctgccaatgtatcgtttccaaggttcttgt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K277002_sequence/1
type: Sequence
persistent identity: BBa K277002 sequence
display id: BBa_K277002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaggttcttgtagagttggttaggcatggtttgtgtccatatggcaccagaaacggaggcaccgatagcagcgcctagctttgaaaaggtatactggatgccggttattacagccatcctactatgcgtagtcatggcttgcagtatgacgatcactgaattgctgcataggagaccactcaaacccatgataacagatgcagcgataacaccttcatgagacccagatcctcctctgtatttgtaaaaaaggcccatgcacaccatccatgcagcacaacctccgattacagaaagtttcagtttccttgtctttgccaccaacaaactgtagaatggagatgcagtagcagcaacaaagtcaggaaggtttactatccgagcagccgaagtggacgattctttcatcgataccagcaaaacaggatacagatagtcacacgaaatgaaaaaggtgaaaaaattgaaaaaagtaacaccaaggggtgcccaaattccacgatcacttagtaatttgaacggtagaagaggagatttggcaaatttggcctcccaatataaaaaaatgaaaaataaacagccacctgaaactaaagtggcaattatttttgaattgtgccacttctgtgatgtctcattagccaacgtcaaagggacaaggatacaccctagcgacacagttattaataatatgccaacaatatcgagtttccagaatagaaacaccaaat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K277003_sequence/1
type: Sequence
persistent identity: BBa K277003 sequence
display id: BBa_K277003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acaccaaattctcaaataatccgcccgttctttcctttctagcctgttctttgagagatctccactcagcagtctttgaagatttgtacttcatataaagaataagaaatataattggcaaggcagagagtgggtaaataaaagcccacattgcaatattccaggaccagtttttctgaggatttgctgctgtgataatattacctgaaatccatggtattatgatatatggccaatatgaggcgtactggtaaaacattctccacttcaaggaggagaaatcagaaagtattaatgtcaggagcagatttgttccgacgtatccacagttatagaaaaccgatcctgctgcatacattgtgagacgggtcgcctgtgattgaatgatggttcccattatataaaaaatagttgcaactaaaaaaagccttagtcttccgaagtggtcagagagtctggagtagacaacttgggatccgacacttacaacagcattgataacttggacagttgaaagtaaggagtgttctgaatatgagttcgtcgcatagcccgtataggtcgatctaagtgtgtagtctaaactaattccaaacccacatacaaacgcggtacttatcagtagaattttatatttcaaggaatcaaactgtgcagacataatttcgttttctttgagcttaaaaaaggtcgatgtcatagagtacgtgtcatcgtttgagtgacgttctcgctcaataatctcacaggactgcctaattccactttttttattactt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K277004_sequence/1
type: Sequence
persistent identity: BBa K277004 sequence
display id: BBa_K277004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attacttgaggcaccaacaacactagaactcatccgataatttatttacttgattattccttttttttttttttctctttccagtccataaaactattttacttttcttatttatgtaaatagtatttaagattctacggtcgctacatctgctagaaatggaattactttaatacattccatgttctcatatatacttttactgacacctttcgtcactattttttgtttaactttttttttggaagtactaaaattatatgctcgtaattattgagtatcaaaaaaaaaaaaaaaaaaaattgctgtgacaccccttcaatgtggtgtctatacacctggtgactttaactttaactttgacctgtcgtcgtacaatcaacttagtttccgaaaatccacgacgaaccagttataccgaagacttttgtacgacaagaatactgtaccgtcgaaactatctcttttcaaactgatgagtgaagcttgtgtttatgtctgttgaaacaaggaaaaaaatgcggtgcctgtcgacaggatatgcgcacaagtgcaaaatcatcaaaaaattgagtacctagatccggtggtgtaggacgagcaaagttccttcataaacaaaccgtaagggttactgatacacaatttcctttttgtaaagagtatttgagcaaattagtcagcgattgaaaacaacacccttttttctatatatagtaggtgaaagttaacatgcgagtaaaagtagtgccacatt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K277005_sequence/1
type: Sequence
persistent identity: BBa K277005 sequence
display id: BBa_K277005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agtgccacatttcctttgcagcaggtataacttcgtataatgtacattatacgaagttataaattggtacatatttaacagatagcgccgcacctcatatgcagcgcgtacatgatacgagacgacaagatatgcaaaagataatagtgtcatcacacctttatgagaagcgaatttttttttttttttggtttaaatatatatatatacatatatatagatatagatatacacatgtatagatgtattctagttatgcttatacctagaactgatcagaccagatcatacctggtatagagcttaatcgatagaaattcaaaaatatggaggaaactaagtactcttcgcagcaggagatagaagaagcatgtggttcagacgcttcattgaatgctagaggtagcaatgattctccaatgggactttccttgtacctctgcctggcttcgtgaactcttgtactattcataactgcactggatattttgatagtgggaactattattgacgtggtcgcagaacagttcggaaactactccaaaacaggttggctcgttacaggctacagtttaccaaatgctattctgagtctcatttggggaagattcgcatctatcataggtttccagcatagtctcattttagcaatacttatttttgaagccggatccctaattgctgcccttgcctcttcaatgaatatgctcattgtcggtagagttgttgctagtgttgggggaagcggact
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K277006_sequence/1
type: Sequence
persistent identity: BBa K277006 sequence
display id: BBa_K277006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aagcggacttcaaacgctttgctttgttattggttgtacgatggttggtgaaaggtcacgtccattggtgatttccatcctaagttgtgcatttgctgtagctgctatcgttggtcctataatcggaggtgcctttacaacccatgttacctggaggtggtgcttctatatcaatcttcctatcggtggtcttgccattattatgtttttactcacatataaggccgagaataagggtatacttcaacaaattaaagatgctataggaacaatctcgagctttacttttagtaagttcagacaccaagttaattttaaaagacttatgaatggcataatcttcaagtttgacttctttggttttgccctctgctctgcagggctggtccttttcctactggggctaacctttggtggtaataaatatagttggaactctggccaagtcatcgcatatttggttttgggtgtcttactttttattttttcattggtgtacgatttcttcttattcgataaattcaacccggaacctgataatatatcctacaggcctctccttctaagaagattggtagcaaaaccagccataataataataaacatggtaacatttctattatgtaccggttacaatgggcaaatgatatactctgtccagtttttccaacttatatttgcgtcgagtgcatggaaagccggtcttcacttgataccaatcgttattaccaacgttattgcggccattgcaagt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K277007_sequence/1
type: Sequence
persistent identity: BBa K277007 sequence
display id: BBa_K277007_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attgcaagtggtgtgattaccaaaaagctcggtttagttaaaccactcttaatatttggaggcgttcttggggtaattggagcagggcttatgacacttatgacaaatacgtccacgaagtcaactcaaattggtgttttgctattaccggggttttcccttggatttgctctacaagcatcgctcatgagtgcacagcttcaaattaccaaagatcgtccagaagccgctatggactttattgaagtaacagctttcaatacattcatgaagtcattaggtacaactcttggtggtgtgctttcaaccactgttttttccgcctcctttcacaacaaagtatcacgagctcatctagagccttacgaaggaaaaacggttgatgacatgattttgtatcgtcttcaaaactacgacggttctcattcgactattggaaacattttaagcgactccattaagaacgtattttggatggatctagggttttatgccttaggatttttgttttgtagtttttcatccaataagaaattaatcataccaaaaaaggacgagacaccagaagataatttagaagacaagtaacgcataacttcgtataatgtacattatacgaagttatagttattttcttattttcattttatttttttcgccttttatacagacttcaacacaatcagaat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K277008_sequence/1
type: Sequence
persistent identity: BBa K277008 sequence
display id: BBa_K277008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aatcagaatcaaataggtgtatcgcaatggaatgtaatttcttaagtattctatatgtacttaaaacctattaatatatggatcaacacagtatcttatgaatgggtttttgatttttttatgtttttttaaaacattaaagttttcggcacggacttatttggaattcaaattattaatgaaagaacaattaactaattaatgtacttagtatttggccattattatcgatttcgggggccaaatctaaccaaattcaacctacattttttcaaattgattcaaacacctttcacaataagatttttatatctagcgcacatagaatgaaatgtaaacaaagatttcagaaaaatcgtataacttcgtataatgtacattatacgaagttatgaaattataagttaaaaagcagtagtttatgctttatgctcgagtatcaagtgaatttgaacaggctagtgcttcattggtacttctttcatggataattttgagcaaatttctgcagcatgtccccctttatacaaattctgtgcattgccggcctagaaatatgtcaacgttttggatatgttgatgcttacttcgagaaatcttacactaatacttctggaaaaaatcaatactagcaaaatagtgatatatgagtaaaatgtatgtagtacatgtatgaaaattatcaagggcaaattgatgcttcaacgaaaaagttattggattttcaagcactttttaaattcacaat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K277009_sequence/1
type: Sequence
persistent identity: BBa K277009 sequence
display id: BBa_K277009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attcacaatatttttttctggaaatatgaaattgtcagcgacttctattacaggagtgtctttttttctcatgctatccaacgcttcttccaaatctttgatggtgttgctgctaccaccgcaagcaataattatcacaatgtcatccgcagctaattttgagcccagtgcattttctaggatcttagtgttgtaacccaaatgcaatgcggcgccacatgccggttcaatcaccatattgaattgatgtgtatatttaagacaggtttcaataacatctttgtcctctattacaacggatctggtgttgtatttgcgagcgtattcgaaagtttggttgctaataacagcggtacccaagctggtagcaatacttgttatcttcttgaattgaactggttggcctattttcaaagaagtattgaaaacatgacatccattcgtttccacccccacaatagggatcctatcagctaaaccatacctttccaaaccttgaataataccattgtataaaccacctccaccaacgctgcatactatgcctttaaccttattcacggaaatatgttgcgatttcaaatcttgtactatttcatctatcatagatgaatgtccttcccaaatatccggattatcgaagggatgaacataaatgggctcaatgacctgagagtctattttattcatgacatttgtttttaaaaaagtatctgcttctttccagtaggcaccactcacgat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K277010_sequence/1
type: Sequence
persistent identity: BBa K277010 sequence
display id: BBa_K277010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: actcacgataacctgggcaccggtgttcctgattttatctaccattctcttctttgtcgctgtaggaaccacgactgtacatggtagagacagtctttgacatgctgttgcagcagcaaaaccggcattaccgccagaactagcgaaaacctgaggagatcttttaccgtccttttgaattcgaatggcacttttcatgatgagattaccgattcctctacttttgaaggagccacttggttgaaggcattcatatttcaagaaaaattgtgcagaagcctttccggggaagaattgacgtaataatggtgttttattgtagactatcgacatctcgctggttaattttcctgtctcttgtctatccagcacttaaaaaagagaaaactgtataaacattttccttttatttggcgatatttatatacctgtgttcctcctcatattgtcccttttatcttatctcatcgcaaacaatttgataaagctttggcactatttaatgacactcagtggaattacatttccgctggggcttaacaggagccgcccatgcggaatcatgtctccgcgcagaggactcattagtttccgttcgcggtgattagtactcgcgaaaagagaaaaagaaaaatagtaggaacatttcgcaattgatcaaaatgtaaacagtgcagtaaagaatgggaccataagatagataagaaacagctcgcataaggaaacaaggacacatcgattaatggcaacccaagccctagaggatatcacggagaggcttttaataaggtcgcaagaggctatcttgcaattagatctgtggatacaacgt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K277011_sequence/1
type: Sequence
persistent identity: BBa K277011 sequence
display id: BBa_K277011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atacaacgtcagcagagatcatcaatatgccagacaacagatcaagagtcattggataagttatcccaacagtacaaccagtatatgtctcaactgaactctttgtatgttagatcggaatctgttcgagacaagttgagcaaggaacaacaacgcagattgatcacagaggataatgagcatcaacgcatagaagacttggttcgtgaattccaggatatcactttgaggttgaacgagctggccaccgtcccaaatgaagcgcctaatgattctccacaatcgcaatccaccagaagtagtttagggtcatttcaacctcgaccattgaaaataattgagaggcaacgtctgtgtatggtaactccatcgaaaccaccaaaaaaatcggtaggctttaaccccatcaatgaagtcgattgtccttcgaaaactaactctttaccgtgctcacctaaaaaacaacctgcaagaaatcgcactttacgtgcagccaaatcacatgatactggcttgaacaaaagtaagaaaccaagcagcagcgacacctacgaaagctttttcaaaaatagacaaagactttcgttgaccttctttgatgaaatggatgatgaagattttgattctgatcaagatactatcattctaccaaacat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K277012_sequence/1
type: Sequence
persistent identity: BBa K277012 sequence
display id: BBa_K277012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atcagatacacatacggtaaaagagaacaacacaaatcttaagcatgcatcttttatggataagtttaattcatcgttaagcacaatatcagaatcttttcaaagtaagagggggagaaagaataagggcatgaatgaagaacgaatatcaaatcataatgtagcacaggaacaaaaaaataatatggatataagcgtctctatagaagaattgcaagatgctttgaatacagaactgctgttttaattaataacttcgtataatgtacattatacgaagttatatgcctcctttacctaacctactcttcttttgctccaaatgtttattctttttttttttattcagaatatcttcaagtttttttgtatctttttgtattattattaaacttctttataatatttgtcttataaaatcctatcataacatgactatggcttggcctagactcgggtgccatcttttttaatgcgactacttcaagacagcttctg
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z