3L.3_23.A2 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns,repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.A2 is comprised of and must be constructed from the parts 3L.3_23.A2.01, 3L.3_23.A2.02, 3L.3_23.A2.03, 3L.3_23.A2.04, 3L.3_23.A2.05, 3L.3_23.A2.06, 3L.3_23.A2.07, 3L.3_23.A2.08, 3L.3_23.A2.09, 3L.3_23.A2.10, 3L.3_23.A2.11, 3L.3_23.A2.12, 3L.3_23.A2.13, and 3L.3_23.A2.14. This part wholly contains the following features (positions offset from first base of sequence): kind and name offset notes reverse_primer YCL057W_tagr1v1 (6353..6380) loxP_site loxPsym_YCL057W (8175..8208) forward_primer YCL059C_tagf1v1 (3862..3889) forward_primer YCL057W_tagf1v1 (6158..6185) reverse_primer YCL055W_tagr1v1 (9956..9983) gene YCL056C (8224..8658) Protein of unknown function%3B green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern loxP_site loxPsym_YCL055W (10239..10272) gene YCL059C (3694..4644) Essential nucleolar protein required for the synthesis of 18S rRNA and for the assembly of 40S ribosomal subunit mutation_affecting_coding_sequence YCL057W_re_remove_AlwNI (6474..6485) removal of AlwNI mutation_affecting_coding_sequence YCL061C_re_remove_AlwNI (1548..1559) removal of AlwNI reverse_primer YCL061C_tagr3v1 (3219..3246) gene YCL058W-A (4849..5190) Protein of unknown function%3B identified by homology to Ashbya gossypii reverse_primer YCL061C_tagr2v1 (2808..2835) reverse_primer YCL061C_tagr1v1 (2601..2628) reverse_primer YCL057W_tagr2v1 (7577..7604) forward_primer YCL061C_tagf3v1 (2991..3018) loxP_site loxPsym_YCL061C (44..77) gene YCL055W (9228..10235) Transcription factor required for induction of KAR3 and CIK1 during mating%2C also required during meiosis%3B exists in two forms%2C a slower-migrating form more abundant during vegetative growth and a faster-migrating form induced by pheromone forward_primer YCL057W_tagf2v1 (7181..7208) gene YCL057W (6033..8171) Zinc metalloendopeptidase%2C found in the cytoplasm and intermembrane space of mitochondria%3B with Cym1p%2C involved in degradation of mitochondrial proteins and of presequence peptides cleaved from imported proteins gene YCL061C (81..3371) S-phase checkpoint protein found at replication forks%2C required for DNA replication%3B also required for Rad53p activation during DNA replication stress%2C where it forms a replication-pausing complex with Tof1p and is phosphorylated by Mec1p%3B protein involved in replication checkpoint forward_primer YCL061C_tagf1v1 (2208..2235) mutation_affecting_coding_sequence YCL058C_stop_recode_overlap_from_YCL058W-A (5190..5190) Overlaps stop codon change in YCL058W-A gene YCL057C-A (5297..5590) Hypothetical protein%2C has similarity to proteins in S. pombe%2C C. elegans%2C D. melanogaster. forward_primer YCL055W_tagf1v1 (9500..9527) reverse_primer YCL059C_tagr1v1 (4312..4336) gene YCL058C (4788..5246) Protein of unknown function%2C required for survival upon exposure to K1 killer toxin%3B involved in ion homeostasis forward_primer YCL061C_tagf2v1 (2337..2364) Sequence (corresponding to coordinates 8871..19174 in synthetic chromosome yeast_chr3_3_23)
Constructed using USER assembly method described in RFC38
3L.3_23.A2.01 is 746 bases long and is cloned into the pGem-T vector. 3L.3_23.A2.01 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.A2.01 is a constituent of 3L.3_23.A2 (along with 3L.3_23.A2.02, 3L.3_23.A2.03, 3L.3_23.A2.04, 3L.3_23.A2.05, 3L.3_23.A2.06, 3L.3_23.A2.07, 3L.3_23.A2.08, 3L.3_23.A2.09, 3L.3_23.A2.10, 3L.3_23.A2.11, 3L.3_23.A2.12, 3L.3_23.A2.13, and 3L.3_23.A2.14.) This part contains at least part of the following features (positions offset from first base of sequence): kind and name offset notes loxP_site loxPsym_YCL061C (44..77) gene YCL061C (81..+3371) S-phase checkpoint protein found at replication forks%2C required for DNA replication%3B also required for Rad53p activation during DNA replication stress%2C where it forms a replication-pausing complex with Tof1p and is phosphorylated by Mec1p%3B protein involved in replication checkpoint Sequence (the first 746 bases correspond to coordinates 8871..9616 in synthetic chromosome yeast_chr3_3_23)
Constructed using overlap extension PCR protocol described in RFC38
3L.3_23.A2.02 is 756 bases long and is cloned into the pGem-T vector. 3L.3_23.A2.02 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.A2.02 is a constituent of 3L.3_23.A2 (along with 3L.3_23.A2.01, 3L.3_23.A2.03, 3L.3_23.A2.04, 3L.3_23.A2.05, 3L.3_23.A2.06, 3L.3_23.A2.07, 3L.3_23.A2.08, 3L.3_23.A2.09, 3L.3_23.A2.10, 3L.3_23.A2.11, 3L.3_23.A2.12, 3L.3_23.A2.13, and 3L.3_23.A2.14.) This part contains at least part of the following features (positions offset from first base of sequence): kind and name offset notes gene YCL061C (-656..+2634) S-phase checkpoint protein found at replication forks%2C required for DNA replication%3B also required for Rad53p activation during DNA replication stress%2C where it forms a replication-pausing complex with Tof1p and is phosphorylated by Mec1p%3B protein involved in replication checkpoint Sequence (the first 756 bases correspond to coordinates 9608..10363 in synthetic chromosome yeast_chr3_3_23)
3L.3_23.A2.03 is 726 bases long and is cloned into the pGem-T vector. 3L.3_23.A2.03 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.A2.03 is a constituent of 3L.3_23.A2 (along with 3L.3_23.A2.01, 3L.3_23.A2.02, 3L.3_23.A2.04, 3L.3_23.A2.05, 3L.3_23.A2.06, 3L.3_23.A2.07, 3L.3_23.A2.08, 3L.3_23.A2.09, 3L.3_23.A2.10, 3L.3_23.A2.11, 3L.3_23.A2.12, 3L.3_23.A2.13, and 3L.3_23.A2.14.) This part contains at least part of the following features (positions offset from first base of sequence): kind and name offset notes mutation_affecting_coding_sequence YCL061C_re_remove_AlwNI (64..75) removal of AlwNI gene YCL061C (-1403..+1887) S-phase checkpoint protein found at replication forks%2C required for DNA replication%3B also required for Rad53p activation during DNA replication stress%2C where it forms a replication-pausing complex with Tof1p and is phosphorylated by Mec1p%3B protein involved in replication checkpoint forward_primer YCL061C_tagf1v1 (724..+751) Sequence (the first 726 bases correspond to coordinates 10355..11080 in synthetic chromosome yeast_chr3_3_23)
3L.3_23.A2.04 is 743 bases long and is cloned into the pGem-T vector. 3L.3_23.A2.04 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.A2.04 is a constituent of 3L.3_23.A2 (along with 3L.3_23.A2.01, 3L.3_23.A2.02, 3L.3_23.A2.03, 3L.3_23.A2.05, 3L.3_23.A2.06, 3L.3_23.A2.07, 3L.3_23.A2.08, 3L.3_23.A2.09, 3L.3_23.A2.10, 3L.3_23.A2.11, 3L.3_23.A2.12, 3L.3_23.A2.13, and 3L.3_23.A2.14.) This part contains at least part of the following features (positions offset from first base of sequence): kind and name offset notes reverse_primer YCL061C_tagr2v1 (607..634) reverse_primer YCL061C_tagr1v1 (400..427) gene YCL061C (-2120..+1170) S-phase checkpoint protein found at replication forks%2C required for DNA replication%3B also required for Rad53p activation during DNA replication stress%2C where it forms a replication-pausing complex with Tof1p and is phosphorylated by Mec1p%3B protein involved in replication checkpoint forward_primer YCL061C_tagf1v1 (7..34) forward_primer YCL061C_tagf2v1 (136..163) Sequence (the first 743 bases correspond to coordinates 11072..11814 in synthetic chromosome yeast_chr3_3_23)
3L.3_23.A2.05 is 741 bases long and is cloned into the pGem-T vector. 3L.3_23.A2.05 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.A2.05 is a constituent of 3L.3_23.A2 (along with 3L.3_23.A2.01, 3L.3_23.A2.02, 3L.3_23.A2.03, 3L.3_23.A2.04, 3L.3_23.A2.06, 3L.3_23.A2.07, 3L.3_23.A2.08, 3L.3_23.A2.09, 3L.3_23.A2.10, 3L.3_23.A2.11, 3L.3_23.A2.12, 3L.3_23.A2.13, and 3L.3_23.A2.14.) This part contains at least part of the following features (positions offset from first base of sequence): kind and name offset notes reverse_primer YCL061C_tagr3v1 (282..309) forward_primer YCL061C_tagf3v1 (54..81) gene YCL061C (-2856..434) S-phase checkpoint protein found at replication forks%2C required for DNA replication%3B also required for Rad53p activation during DNA replication stress%2C where it forms a replication-pausing complex with Tof1p and is phosphorylated by Mec1p%3B protein involved in replication checkpoint Sequence (the first 741 bases correspond to coordinates 11808..12548 in synthetic chromosome yeast_chr3_3_23)
3L.3_23.A2.06 is 749 bases long and is cloned into the pGem-T vector. 3L.3_23.A2.06 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.A2.06 is a constituent of 3L.3_23.A2 (along with 3L.3_23.A2.01, 3L.3_23.A2.02, 3L.3_23.A2.03, 3L.3_23.A2.04, 3L.3_23.A2.05, 3L.3_23.A2.07, 3L.3_23.A2.08, 3L.3_23.A2.09, 3L.3_23.A2.10, 3L.3_23.A2.11, 3L.3_23.A2.12, 3L.3_23.A2.13, and 3L.3_23.A2.14.) This part contains at least part of the following features (positions offset from first base of sequence): kind and name offset notes forward_primer YCL059C_tagf1v1 (191..218) gene YCL059C (23..+973) Essential nucleolar protein required for the synthesis of 18S rRNA and for the assembly of 40S ribosomal subunit reverse_primer YCL059C_tagr1v1 (641..665) Sequence (the first 749 bases correspond to coordinates 12542..13290 in synthetic chromosome yeast_chr3_3_23)
3L.3_23.A2.07 is 815 bases long and is cloned into the pGem-T vector. 3L.3_23.A2.07 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.A2.07 is a constituent of 3L.3_23.A2 (along with 3L.3_23.A2.01, 3L.3_23.A2.02, 3L.3_23.A2.03, 3L.3_23.A2.04, 3L.3_23.A2.05, 3L.3_23.A2.06, 3L.3_23.A2.08, 3L.3_23.A2.09, 3L.3_23.A2.10, 3L.3_23.A2.11, 3L.3_23.A2.12, 3L.3_23.A2.13, and 3L.3_23.A2.14.) This part contains at least part of the following features (positions offset from first base of sequence): kind and name offset notes gene YCL059C (-715..235) Essential nucleolar protein required for the synthesis of 18S rRNA and for the assembly of 40S ribosomal subunit gene YCL058W-A (440..781) Protein of unknown function%3B identified by homology to Ashbya gossypii mutation_affecting_coding_sequence YCL058C_stop_recode_overlap_from_YCL058W-A (781..781) Overlaps stop codon change in YCL058W-A gene YCL058C (379..+837) Protein of unknown function%2C required for survival upon exposure to K1 killer toxin%3B involved in ion homeostasis Sequence (the first 815 bases correspond to coordinates 13280..14094 in synthetic chromosome yeast_chr3_3_23)
3L.3_23.A2.08 is 674 bases long and is cloned into the pGem-T vector. 3L.3_23.A2.08 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.A2.08 is a constituent of 3L.3_23.A2 (along with 3L.3_23.A2.01, 3L.3_23.A2.02, 3L.3_23.A2.03, 3L.3_23.A2.04, 3L.3_23.A2.05, 3L.3_23.A2.06, 3L.3_23.A2.07, 3L.3_23.A2.09, 3L.3_23.A2.10, 3L.3_23.A2.11, 3L.3_23.A2.12, 3L.3_23.A2.13, and 3L.3_23.A2.14.) This part contains at least part of the following features (positions offset from first base of sequence): kind and name offset notes gene YCL057C-A (84..377) Hypothetical protein%2C has similarity to proteins in S. pombe%2C C. elegans%2C D. melanogaster. gene YCL058C (-425..33) Protein of unknown function%2C required for survival upon exposure to K1 killer toxin%3B involved in ion homeostasis Sequence (the first 674 bases correspond to coordinates 14084..14757 in synthetic chromosome yeast_chr3_3_23)
3L.3_23.A2.09 is 839 bases long and is cloned into the pGem-T vector. 3L.3_23.A2.09 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.A2.09 is a constituent of 3L.3_23.A2 (along with 3L.3_23.A2.01, 3L.3_23.A2.02, 3L.3_23.A2.03, 3L.3_23.A2.04, 3L.3_23.A2.05, 3L.3_23.A2.06, 3L.3_23.A2.07, 3L.3_23.A2.08, 3L.3_23.A2.10, 3L.3_23.A2.11, 3L.3_23.A2.12, 3L.3_23.A2.13, and 3L.3_23.A2.14.) This part contains at least part of the following features (positions offset from first base of sequence): kind and name offset notes reverse_primer YCL057W_tagr1v1 (473..500) forward_primer YCL057W_tagf1v1 (278..305) mutation_affecting_coding_sequence YCL057W_re_remove_AlwNI (594..605) removal of AlwNI gene YCL057W (153..+2291) Zinc metalloendopeptidase%2C found in the cytoplasm and intermembrane space of mitochondria%3B with Cym1p%2C involved in degradation of mitochondrial proteins and of presequence peptides cleaved from imported proteins Sequence (the first 839 bases correspond to coordinates 14751..15589 in synthetic chromosome yeast_chr3_3_23)
3L.3_23.A2.10 is 748 bases long and is cloned into the pGem-T vector. 3L.3_23.A2.10 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.A2.10 is a constituent of 3L.3_23.A2 (along with 3L.3_23.A2.01, 3L.3_23.A2.02, 3L.3_23.A2.03, 3L.3_23.A2.04, 3L.3_23.A2.05, 3L.3_23.A2.06, 3L.3_23.A2.07, 3L.3_23.A2.08, 3L.3_23.A2.09, 3L.3_23.A2.11, 3L.3_23.A2.12, 3L.3_23.A2.13, and 3L.3_23.A2.14.) This part contains at least part of the following features (positions offset from first base of sequence): kind and name offset notes forward_primer YCL057W_tagf2v1 (471..498) gene YCL057W (-677..+1461) Zinc metalloendopeptidase%2C found in the cytoplasm and intermembrane space of mitochondria%3B with Cym1p%2C involved in degradation of mitochondrial proteins and of presequence peptides cleaved from imported proteins Sequence (the first 748 bases correspond to coordinates 15581..16328 in synthetic chromosome yeast_chr3_3_23)
3L.3_23.A2.11 is 645 bases long and is cloned into the pGem-T vector. 3L.3_23.A2.11 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.A2.11 is a constituent of 3L.3_23.A2 (along with 3L.3_23.A2.01, 3L.3_23.A2.02, 3L.3_23.A2.03, 3L.3_23.A2.04, 3L.3_23.A2.05, 3L.3_23.A2.06, 3L.3_23.A2.07, 3L.3_23.A2.08, 3L.3_23.A2.09, 3L.3_23.A2.10, 3L.3_23.A2.12, 3L.3_23.A2.13, and 3L.3_23.A2.14.) This part contains at least part of the following features (positions offset from first base of sequence): kind and name offset notes reverse_primer YCL057W_tagr2v1 (126..153) gene YCL057W (-1418..+720) Zinc metalloendopeptidase%2C found in the cytoplasm and intermembrane space of mitochondria%3B with Cym1p%2C involved in degradation of mitochondrial proteins and of presequence peptides cleaved from imported proteins Sequence (the first 645 bases correspond to coordinates 16322..16966 in synthetic chromosome yeast_chr3_3_23):
3L.3_23.A2.12 is 742 bases long and is cloned into the pGem-T vector. 3L.3_23.A2.12 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.A2.12 is a constituent of 3L.3_23.A2 (along with 3L.3_23.A2.01, 3L.3_23.A2.02, 3L.3_23.A2.03, 3L.3_23.A2.04, 3L.3_23.A2.05, 3L.3_23.A2.06, 3L.3_23.A2.07, 3L.3_23.A2.08, 3L.3_23.A2.09, 3L.3_23.A2.10, 3L.3_23.A2.11, 3L.3_23.A2.13, and 3L.3_23.A2.14.) This part contains at least part of the following features (positions offset from first base of sequence): kind and name offset notes loxP_site loxPsym_YCL057W (90..123) gene YCL056C (139..573) Protein of unknown function%3B green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern gene YCL057W (-2052..86) Zinc metalloendopeptidase%2C found in the cytoplasm and intermembrane space of mitochondria%3B with Cym1p%2C involved in degradation of mitochondrial proteins and of presequence peptides cleaved from imported proteins Sequence (the first 742 bases correspond to coordinates 16956..17697 in synthetic chromosome yeast_chr3_3_23)
3L.3_23.A2.13 is 747 bases long and is cloned into the pGem-T vector. 3L.3_23.A2.13 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.A2.13 is a constituent of 3L.3_23.A2 (along with 3L.3_23.A2.01, 3L.3_23.A2.02, 3L.3_23.A2.03, 3L.3_23.A2.04, 3L.3_23.A2.05, 3L.3_23.A2.06, 3L.3_23.A2.07, 3L.3_23.A2.08, 3L.3_23.A2.09, 3L.3_23.A2.10, 3L.3_23.A2.11, 3L.3_23.A2.12, and 3L.3_23.A2.14.) This part contains at least part of the following features (positions offset from first base of sequence): kind and name offset notes gene YCL055W (410..+1417) Transcription factor required for induction of KAR3 and CIK1 during mating%2C also required during meiosis%3B exists in two forms%2C a slower-migrating form more abundant during vegetative growth and a faster-migrating form induced by pheromone forward_primer YCL055W_tagf1v1 (682..709) Sequence (the first 747 bases correspond to coordinates 17689..18435 in synthetic chromosome yeast_chr3_3_23)
3L.3_23.A2.14 is 750 bases long and is cloned into the pGem-T vector. 3L.3_23.A2.14 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.A2.14 is a constituent of 3L.3_23.A2 (along with 3L.3_23.A2.01, 3L.3_23.A2.02, 3L.3_23.A2.03, 3L.3_23.A2.04, 3L.3_23.A2.05, 3L.3_23.A2.06, 3L.3_23.A2.07, 3L.3_23.A2.08, 3L.3_23.A2.09, 3L.3_23.A2.10, 3L.3_23.A2.11, 3L.3_23.A2.12, and 3L.3_23.A2.13.) This part contains at least part of the following features (positions offset from first base of sequence): kind and name offset notes reverse_primer YCL055W_tagr1v1 (402..429) loxP_site loxPsym_YCL055W (685..718) gene YCL055W (-326..681) Transcription factor required for induction of KAR3 and CIK1 during mating%2C also required during meiosis%3B exists in two forms%2C a slower-migrating form more abundant during vegetative growth and a faster-migrating form induced by pheromone Sequence (the first 750 bases correspond to coordinates 18425..19174 in synthetic chromosome yeast_chr3_3_23)
Conversion of the iGEM parts registry to SBOL2.1