BBa_K277135
type: ComponentDefinition
persistent identity: BBa K277135
display id: BBa_K277135
version: 1
was generated by: iGEM to SBOL conversion
description: 3L.3_23.A2
created: 2009-10-20T11:00:00Z
modified: 2015-05-08T01:11:47Z
mutable provenance: Synthetic Yeast
owned by: myers
owned by: james
mutable description: 3L.3_23.A2 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns,repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.A2 is comprised of and must be constructed from the parts 3L.3_23.A2.01, 3L.3_23.A2.02, 3L.3_23.A2.03, 3L.3_23.A2.04, 3L.3_23.A2.05, 3L.3_23.A2.06, 3L.3_23.A2.07, 3L.3_23.A2.08, 3L.3_23.A2.09, 3L.3_23.A2.10, 3L.3_23.A2.11, 3L.3_23.A2.12, 3L.3_23.A2.13, and 3L.3_23.A2.14. This part wholly contains the following features (positions offset from first base of sequence): kind and name offset notes reverse_primer YCL057W_tagr1v1 (6353..6380) loxP_site loxPsym_YCL057W (8175..8208) forward_primer YCL059C_tagf1v1 (3862..3889) forward_primer YCL057W_tagf1v1 (6158..6185) reverse_primer YCL055W_tagr1v1 (9956..9983) gene YCL056C (8224..8658) Protein of unknown function%3B green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern loxP_site loxPsym_YCL055W (10239..10272) gene YCL059C (3694..4644) Essential nucleolar protein required for the synthesis of 18S rRNA and for the assembly of 40S ribosomal subunit mutation_affecting_coding_sequence YCL057W_re_remove_AlwNI (6474..6485) removal of AlwNI mutation_affecting_coding_sequence YCL061C_re_remove_AlwNI (1548..1559) removal of AlwNI reverse_primer YCL061C_tagr3v1 (3219..3246) gene YCL058W-A (4849..5190) Protein of unknown function%3B identified by homology to Ashbya gossypii reverse_primer YCL061C_tagr2v1 (2808..2835) reverse_primer YCL061C_tagr1v1 (2601..2628) reverse_primer YCL057W_tagr2v1 (7577..7604) forwar…
discontinued: false
dominant: false
group u list: _385_
m user id: 0
owner id: 2669
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Constructed using USER assembly method described in RFC38
star: false
creator: James DiCarlo
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K277019
component: BBa_K277018
component: BBa_K277021
component: BBa_K277020
component: BBa_K277023
component: BBa_K277022
component: BBa_K277025
component: BBa_K277024
component: BBa_K277013
component: BBa_K277015
component: BBa_K277014
component: BBa_K277017
component: BBa_K277016
component: BBa_K277026
sequence annotation: BBa_K277013
sequence annotation: BBa_K277014
sequence annotation: BBa_K277015
sequence annotation: BBa_K277016
sequence annotation: BBa_K277017
sequence annotation: BBa_K277018
sequence annotation: BBa_K277019
sequence annotation: BBa_K277020
sequence annotation: BBa_K277021
sequence annotation: BBa_K277022
sequence annotation: BBa_K277023
sequence annotation: BBa_K277024
sequence annotation: BBa_K277025
sequence annotation: BBa_K277026
sequence: https://synbiohub.org/public/igem/BBa_K277135_sequence/1

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BBa_K277018
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BBa_K277015
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BBa_K277014
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BBa_K277017
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BBa_K277018
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BBa_K277019
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BBa_K277020
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BBa_K277021
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BBa_K277022
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BBa_K277023
type: SequenceAnnotation
persistent identity: annotation2062001
display id: annotation2062001
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BBa_K277024
type: SequenceAnnotation
persistent identity: annotation2062002
display id: annotation2062002
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BBa_K277025
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display id: annotation2062003
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BBa_K277026
type: SequenceAnnotation
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BBa_K277013
type: ComponentDefinition
persistent identity: BBa K277013
display id: BBa_K277013
version: 1
was generated by: iGEM to SBOL conversion
description: 3L.3_23.A2.01
created: 2009-10-19T11:00:00Z
modified: 2015-05-08T01:11:45Z
mutable provenance: Synthetic Yeast
owned by: james
owned by: myers
mutable description: 3L.3_23.A2.01 is 746 bases long and is cloned into the pGem-T vector. 3L.3_23.A2.01 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.A2.01 is a constituent of 3L.3_23.A2 (along with 3L.3_23.A2.02, 3L.3_23.A2.03, 3L.3_23.A2.04, 3L.3_23.A2.05, 3L.3_23.A2.06, 3L.3_23.A2.07, 3L.3_23.A2.08, 3L.3_23.A2.09, 3L.3_23.A2.10, 3L.3_23.A2.11, 3L.3_23.A2.12, 3L.3_23.A2.13, and 3L.3_23.A2.14.) This part contains at least part of the following features (positions offset from first base of sequence): kind and name offset notes loxP_site loxPsym_YCL061C (44..77) gene YCL061C (81..+3371) S-phase checkpoint protein found at replication forks%2C required for DNA replication%3B also required for Rad53p activation during DNA replication stress%2C where it forms a replication-pausing complex with Tof1p and is phosphorylated by Mec1p%3B protein involved in replication checkpoint Sequence (the first 746 bases correspond to coordinates 8871..9616 in synthetic chromosome yeast_chr3_3_23)
discontinued: false
dominant: false
group u list: _385_
m user id: 0
owner id: 2669
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Constructed using overlap extension PCR protocol described in RFC38
star: false
creator: James DiCarlo
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K277013_sequence/1

BBa_K277014
type: ComponentDefinition
persistent identity: BBa K277014
display id: BBa_K277014
version: 1
was generated by: iGEM to SBOL conversion
description: 3L.3_23.A2.02
created: 2009-10-19T11:00:00Z
modified: 2015-05-08T01:11:45Z
mutable provenance: Synthetic Yeast
owned by: james
owned by: myers
mutable description: 3L.3_23.A2.02 is 756 bases long and is cloned into the pGem-T vector. 3L.3_23.A2.02 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.A2.02 is a constituent of 3L.3_23.A2 (along with 3L.3_23.A2.01, 3L.3_23.A2.03, 3L.3_23.A2.04, 3L.3_23.A2.05, 3L.3_23.A2.06, 3L.3_23.A2.07, 3L.3_23.A2.08, 3L.3_23.A2.09, 3L.3_23.A2.10, 3L.3_23.A2.11, 3L.3_23.A2.12, 3L.3_23.A2.13, and 3L.3_23.A2.14.) This part contains at least part of the following features (positions offset from first base of sequence): kind and name offset notes gene YCL061C (-656..+2634) S-phase checkpoint protein found at replication forks%2C required for DNA replication%3B also required for Rad53p activation during DNA replication stress%2C where it forms a replication-pausing complex with Tof1p and is phosphorylated by Mec1p%3B protein involved in replication checkpoint Sequence (the first 756 bases correspond to coordinates 9608..10363 in synthetic chromosome yeast_chr3_3_23)
discontinued: false
dominant: false
group u list: _385_
m user id: 0
owner id: 2669
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Constructed using overlap extension PCR protocol described in RFC38
star: false
creator: James DiCarlo
type: DnaRegion
role: SO:0000110
role: partType/Other
sequence: https://synbiohub.org/public/igem/BBa_K277014_sequence/1

BBa_K277015
type: ComponentDefinition
persistent identity: BBa K277015
display id: BBa_K277015
version: 1
was generated by: iGEM to SBOL conversion
description: 3L.3_23.A2.03
created: 2009-10-19T11:00:00Z
modified: 2015-05-08T01:11:45Z
mutable provenance: Synthetic Yeast
owned by: james
owned by: myers
mutable description: 3L.3_23.A2.03 is 726 bases long and is cloned into the pGem-T vector. 3L.3_23.A2.03 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.A2.03 is a constituent of 3L.3_23.A2 (along with 3L.3_23.A2.01, 3L.3_23.A2.02, 3L.3_23.A2.04, 3L.3_23.A2.05, 3L.3_23.A2.06, 3L.3_23.A2.07, 3L.3_23.A2.08, 3L.3_23.A2.09, 3L.3_23.A2.10, 3L.3_23.A2.11, 3L.3_23.A2.12, 3L.3_23.A2.13, and 3L.3_23.A2.14.) This part contains at least part of the following features (positions offset from first base of sequence): kind and name offset notes mutation_affecting_coding_sequence YCL061C_re_remove_AlwNI (64..75) removal of AlwNI gene YCL061C (-1403..+1887) S-phase checkpoint protein found at replication forks%2C required for DNA replication%3B also required for Rad53p activation during DNA replication stress%2C where it forms a replication-pausing complex with Tof1p and is phosphorylated by Mec1p%3B protein involved in replication checkpoint forward_primer YCL061C_tagf1v1 (724..+751) Sequence (the first 726 bases correspond to coordinates 10355..11080 in synthetic chromosome yeast_chr3_3_23)
discontinued: false
dominant: false
group u list: _385_
m user id: 0
owner id: 2669
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Constructed using overlap extension PCR protocol described in RFC38
star: false
creator: James DiCarlo
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K277015_sequence/1

BBa_K277016
type: ComponentDefinition
persistent identity: BBa K277016
display id: BBa_K277016
version: 1
was generated by: iGEM to SBOL conversion
description: 3L.3_23.A2.04
created: 2009-10-19T11:00:00Z
modified: 2015-05-08T01:11:45Z
mutable provenance: Synthetic Yeast
owned by: james
owned by: myers
mutable description: 3L.3_23.A2.04 is 743 bases long and is cloned into the pGem-T vector. 3L.3_23.A2.04 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.A2.04 is a constituent of 3L.3_23.A2 (along with 3L.3_23.A2.01, 3L.3_23.A2.02, 3L.3_23.A2.03, 3L.3_23.A2.05, 3L.3_23.A2.06, 3L.3_23.A2.07, 3L.3_23.A2.08, 3L.3_23.A2.09, 3L.3_23.A2.10, 3L.3_23.A2.11, 3L.3_23.A2.12, 3L.3_23.A2.13, and 3L.3_23.A2.14.) This part contains at least part of the following features (positions offset from first base of sequence): kind and name offset notes reverse_primer YCL061C_tagr2v1 (607..634) reverse_primer YCL061C_tagr1v1 (400..427) gene YCL061C (-2120..+1170) S-phase checkpoint protein found at replication forks%2C required for DNA replication%3B also required for Rad53p activation during DNA replication stress%2C where it forms a replication-pausing complex with Tof1p and is phosphorylated by Mec1p%3B protein involved in replication checkpoint forward_primer YCL061C_tagf1v1 (7..34) forward_primer YCL061C_tagf2v1 (136..163) Sequence (the first 743 bases correspond to coordinates 11072..11814 in synthetic chromosome yeast_chr3_3_23)
discontinued: false
dominant: false
group u list: _385_
m user id: 0
owner id: 2669
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Constructed using overlap extension PCR protocol described in RFC38
star: false
creator: James DiCarlo
type: DnaRegion
role: SO:0000110
role: partType/Other
sequence: https://synbiohub.org/public/igem/BBa_K277016_sequence/1

BBa_K277017
type: ComponentDefinition
persistent identity: BBa K277017
display id: BBa_K277017
version: 1
was generated by: iGEM to SBOL conversion
description: 3L.3_23.A2.05
created: 2009-10-19T11:00:00Z
modified: 2015-05-08T01:11:45Z
mutable provenance: Synthetic Yeast
owned by: james
owned by: myers
mutable description: 3L.3_23.A2.05 is 741 bases long and is cloned into the pGem-T vector. 3L.3_23.A2.05 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.A2.05 is a constituent of 3L.3_23.A2 (along with 3L.3_23.A2.01, 3L.3_23.A2.02, 3L.3_23.A2.03, 3L.3_23.A2.04, 3L.3_23.A2.06, 3L.3_23.A2.07, 3L.3_23.A2.08, 3L.3_23.A2.09, 3L.3_23.A2.10, 3L.3_23.A2.11, 3L.3_23.A2.12, 3L.3_23.A2.13, and 3L.3_23.A2.14.) This part contains at least part of the following features (positions offset from first base of sequence): kind and name offset notes reverse_primer YCL061C_tagr3v1 (282..309) forward_primer YCL061C_tagf3v1 (54..81) gene YCL061C (-2856..434) S-phase checkpoint protein found at replication forks%2C required for DNA replication%3B also required for Rad53p activation during DNA replication stress%2C where it forms a replication-pausing complex with Tof1p and is phosphorylated by Mec1p%3B protein involved in replication checkpoint Sequence (the first 741 bases correspond to coordinates 11808..12548 in synthetic chromosome yeast_chr3_3_23)
discontinued: false
dominant: false
group u list: _385_
m user id: 0
owner id: 2669
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Constructed using overlap extension PCR protocol described in RFC38
star: false
creator: James DiCarlo
type: DnaRegion
role: SO:0000110
role: partType/Other
sequence: https://synbiohub.org/public/igem/BBa_K277017_sequence/1

BBa_K277018
type: ComponentDefinition
persistent identity: BBa K277018
display id: BBa_K277018
version: 1
was generated by: iGEM to SBOL conversion
description: 3L.3_23.A2.06
created: 2009-10-19T11:00:00Z
modified: 2015-05-08T01:11:45Z
mutable provenance: Synthetic Yeast
owned by: james
owned by: myers
mutable description: 3L.3_23.A2.06 is 749 bases long and is cloned into the pGem-T vector. 3L.3_23.A2.06 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.A2.06 is a constituent of 3L.3_23.A2 (along with 3L.3_23.A2.01, 3L.3_23.A2.02, 3L.3_23.A2.03, 3L.3_23.A2.04, 3L.3_23.A2.05, 3L.3_23.A2.07, 3L.3_23.A2.08, 3L.3_23.A2.09, 3L.3_23.A2.10, 3L.3_23.A2.11, 3L.3_23.A2.12, 3L.3_23.A2.13, and 3L.3_23.A2.14.) This part contains at least part of the following features (positions offset from first base of sequence): kind and name offset notes forward_primer YCL059C_tagf1v1 (191..218) gene YCL059C (23..+973) Essential nucleolar protein required for the synthesis of 18S rRNA and for the assembly of 40S ribosomal subunit reverse_primer YCL059C_tagr1v1 (641..665) Sequence (the first 749 bases correspond to coordinates 12542..13290 in synthetic chromosome yeast_chr3_3_23)
discontinued: false
dominant: false
group u list: _385_
m user id: 0
owner id: 2669
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Constructed using overlap extension PCR protocol described in RFC38
star: false
creator: James DiCarlo
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K277018_sequence/1

BBa_K277019
type: ComponentDefinition
persistent identity: BBa K277019
display id: BBa_K277019
version: 1
was generated by: iGEM to SBOL conversion
description: 3L.3_23.A2.07
created: 2009-10-19T11:00:00Z
modified: 2015-05-08T01:11:45Z
mutable provenance: Synthetic Yeast
owned by: james
owned by: myers
mutable description: 3L.3_23.A2.07 is 815 bases long and is cloned into the pGem-T vector. 3L.3_23.A2.07 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.A2.07 is a constituent of 3L.3_23.A2 (along with 3L.3_23.A2.01, 3L.3_23.A2.02, 3L.3_23.A2.03, 3L.3_23.A2.04, 3L.3_23.A2.05, 3L.3_23.A2.06, 3L.3_23.A2.08, 3L.3_23.A2.09, 3L.3_23.A2.10, 3L.3_23.A2.11, 3L.3_23.A2.12, 3L.3_23.A2.13, and 3L.3_23.A2.14.) This part contains at least part of the following features (positions offset from first base of sequence): kind and name offset notes gene YCL059C (-715..235) Essential nucleolar protein required for the synthesis of 18S rRNA and for the assembly of 40S ribosomal subunit gene YCL058W-A (440..781) Protein of unknown function%3B identified by homology to Ashbya gossypii mutation_affecting_coding_sequence YCL058C_stop_recode_overlap_from_YCL058W-A (781..781) Overlaps stop codon change in YCL058W-A gene YCL058C (379..+837) Protein of unknown function%2C required for survival upon exposure to K1 killer toxin%3B involved in ion homeostasis Sequence (the first 815 bases correspond to coordinates 13280..14094 in synthetic chromosome yeast_chr3_3_23)
discontinued: false
dominant: false
group u list: _385_
m user id: 0
owner id: 2669
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Constructed using overlap extension PCR protocol described in RFC38
star: false
creator: James DiCarlo
type: DnaRegion
role: SO:0000110
role: partType/Other
sequence: https://synbiohub.org/public/igem/BBa_K277019_sequence/1

BBa_K277020
type: ComponentDefinition
persistent identity: BBa K277020
display id: BBa_K277020
version: 1
was generated by: iGEM to SBOL conversion
description: 3L.3_23.A2.08
created: 2009-10-19T11:00:00Z
modified: 2015-05-08T01:11:45Z
mutable provenance: Synthetic Yeast
owned by: james
owned by: myers
mutable description: 3L.3_23.A2.08 is 674 bases long and is cloned into the pGem-T vector. 3L.3_23.A2.08 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.A2.08 is a constituent of 3L.3_23.A2 (along with 3L.3_23.A2.01, 3L.3_23.A2.02, 3L.3_23.A2.03, 3L.3_23.A2.04, 3L.3_23.A2.05, 3L.3_23.A2.06, 3L.3_23.A2.07, 3L.3_23.A2.09, 3L.3_23.A2.10, 3L.3_23.A2.11, 3L.3_23.A2.12, 3L.3_23.A2.13, and 3L.3_23.A2.14.) This part contains at least part of the following features (positions offset from first base of sequence): kind and name offset notes gene YCL057C-A (84..377) Hypothetical protein%2C has similarity to proteins in S. pombe%2C C. elegans%2C D. melanogaster. gene YCL058C (-425..33) Protein of unknown function%2C required for survival upon exposure to K1 killer toxin%3B involved in ion homeostasis Sequence (the first 674 bases correspond to coordinates 14084..14757 in synthetic chromosome yeast_chr3_3_23)
discontinued: false
dominant: false
group u list: _385_
m user id: 0
owner id: 2669
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Constructed using overlap extension PCR protocol described in RFC38
star: false
creator: James DiCarlo
type: DnaRegion
role: SO:0000110
role: partType/Other
sequence: https://synbiohub.org/public/igem/BBa_K277020_sequence/1

BBa_K277021
type: ComponentDefinition
persistent identity: BBa K277021
display id: BBa_K277021
version: 1
was generated by: iGEM to SBOL conversion
description: 3L.3_23.A2.09
created: 2009-10-19T11:00:00Z
modified: 2015-05-08T01:11:45Z
mutable provenance: Synthetic Yeast
owned by: james
owned by: myers
mutable description: 3L.3_23.A2.09 is 839 bases long and is cloned into the pGem-T vector. 3L.3_23.A2.09 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.A2.09 is a constituent of 3L.3_23.A2 (along with 3L.3_23.A2.01, 3L.3_23.A2.02, 3L.3_23.A2.03, 3L.3_23.A2.04, 3L.3_23.A2.05, 3L.3_23.A2.06, 3L.3_23.A2.07, 3L.3_23.A2.08, 3L.3_23.A2.10, 3L.3_23.A2.11, 3L.3_23.A2.12, 3L.3_23.A2.13, and 3L.3_23.A2.14.) This part contains at least part of the following features (positions offset from first base of sequence): kind and name offset notes reverse_primer YCL057W_tagr1v1 (473..500) forward_primer YCL057W_tagf1v1 (278..305) mutation_affecting_coding_sequence YCL057W_re_remove_AlwNI (594..605) removal of AlwNI gene YCL057W (153..+2291) Zinc metalloendopeptidase%2C found in the cytoplasm and intermembrane space of mitochondria%3B with Cym1p%2C involved in degradation of mitochondrial proteins and of presequence peptides cleaved from imported proteins Sequence (the first 839 bases correspond to coordinates 14751..15589 in synthetic chromosome yeast_chr3_3_23)
discontinued: false
dominant: false
group u list: _385_
m user id: 0
owner id: 2669
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Constructed using overlap extension PCR protocol described in RFC38
star: false
creator: James DiCarlo
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K277021_sequence/1

BBa_K277022
type: ComponentDefinition
persistent identity: BBa K277022
display id: BBa_K277022
version: 1
was generated by: iGEM to SBOL conversion
description: 3L.3_23.A2.10
created: 2009-10-19T11:00:00Z
modified: 2015-05-08T01:11:45Z
mutable provenance: Synthetic Yeast
owned by: james
owned by: myers
mutable description: 3L.3_23.A2.10 is 748 bases long and is cloned into the pGem-T vector. 3L.3_23.A2.10 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.A2.10 is a constituent of 3L.3_23.A2 (along with 3L.3_23.A2.01, 3L.3_23.A2.02, 3L.3_23.A2.03, 3L.3_23.A2.04, 3L.3_23.A2.05, 3L.3_23.A2.06, 3L.3_23.A2.07, 3L.3_23.A2.08, 3L.3_23.A2.09, 3L.3_23.A2.11, 3L.3_23.A2.12, 3L.3_23.A2.13, and 3L.3_23.A2.14.) This part contains at least part of the following features (positions offset from first base of sequence): kind and name offset notes forward_primer YCL057W_tagf2v1 (471..498) gene YCL057W (-677..+1461) Zinc metalloendopeptidase%2C found in the cytoplasm and intermembrane space of mitochondria%3B with Cym1p%2C involved in degradation of mitochondrial proteins and of presequence peptides cleaved from imported proteins Sequence (the first 748 bases correspond to coordinates 15581..16328 in synthetic chromosome yeast_chr3_3_23)
discontinued: false
dominant: false
group u list: _385_
m user id: 0
owner id: 2669
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Constructed using overlap extension PCR protocol described in RFC38
star: false
creator: James DiCarlo
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K277022_sequence/1

BBa_K277023
type: ComponentDefinition
persistent identity: BBa K277023
display id: BBa_K277023
version: 1
was generated by: iGEM to SBOL conversion
description: 3L.3_23.A2.11
created: 2009-10-19T11:00:00Z
modified: 2015-05-08T01:11:45Z
mutable provenance: Synthetic Yeast
owned by: james
owned by: myers
mutable description: 3L.3_23.A2.11 is 645 bases long and is cloned into the pGem-T vector. 3L.3_23.A2.11 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.A2.11 is a constituent of 3L.3_23.A2 (along with 3L.3_23.A2.01, 3L.3_23.A2.02, 3L.3_23.A2.03, 3L.3_23.A2.04, 3L.3_23.A2.05, 3L.3_23.A2.06, 3L.3_23.A2.07, 3L.3_23.A2.08, 3L.3_23.A2.09, 3L.3_23.A2.10, 3L.3_23.A2.12, 3L.3_23.A2.13, and 3L.3_23.A2.14.) This part contains at least part of the following features (positions offset from first base of sequence): kind and name offset notes reverse_primer YCL057W_tagr2v1 (126..153) gene YCL057W (-1418..+720) Zinc metalloendopeptidase%2C found in the cytoplasm and intermembrane space of mitochondria%3B with Cym1p%2C involved in degradation of mitochondrial proteins and of presequence peptides cleaved from imported proteins Sequence (the first 645 bases correspond to coordinates 16322..16966 in synthetic chromosome yeast_chr3_3_23):
discontinued: false
dominant: false
group u list: _385_
m user id: 0
owner id: 2669
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Constructed using overlap extension PCR protocol described in RFC38
star: false
creator: James DiCarlo
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K277023_sequence/1

BBa_K277024
type: ComponentDefinition
persistent identity: BBa K277024
display id: BBa_K277024
version: 1
was generated by: iGEM to SBOL conversion
description: 3L.3_23.A2.12
created: 2009-10-19T11:00:00Z
modified: 2015-05-08T01:11:46Z
mutable provenance: Synthetic Yeast
owned by: james
owned by: myers
mutable description: 3L.3_23.A2.12 is 742 bases long and is cloned into the pGem-T vector. 3L.3_23.A2.12 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.A2.12 is a constituent of 3L.3_23.A2 (along with 3L.3_23.A2.01, 3L.3_23.A2.02, 3L.3_23.A2.03, 3L.3_23.A2.04, 3L.3_23.A2.05, 3L.3_23.A2.06, 3L.3_23.A2.07, 3L.3_23.A2.08, 3L.3_23.A2.09, 3L.3_23.A2.10, 3L.3_23.A2.11, 3L.3_23.A2.13, and 3L.3_23.A2.14.) This part contains at least part of the following features (positions offset from first base of sequence): kind and name offset notes loxP_site loxPsym_YCL057W (90..123) gene YCL056C (139..573) Protein of unknown function%3B green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern gene YCL057W (-2052..86) Zinc metalloendopeptidase%2C found in the cytoplasm and intermembrane space of mitochondria%3B with Cym1p%2C involved in degradation of mitochondrial proteins and of presequence peptides cleaved from imported proteins Sequence (the first 742 bases correspond to coordinates 16956..17697 in synthetic chromosome yeast_chr3_3_23)
discontinued: false
dominant: false
group u list: _385_
m user id: 0
owner id: 2669
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Constructed using overlap extension PCR protocol described in RFC38
star: false
creator: James DiCarlo
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K277024_sequence/1

BBa_K277025
type: ComponentDefinition
persistent identity: BBa K277025
display id: BBa_K277025
version: 1
was generated by: iGEM to SBOL conversion
description: 3L.3_23.A2.13
created: 2009-10-19T11:00:00Z
modified: 2015-05-08T01:11:46Z
mutable provenance: Synthetic Yeast
owned by: james
owned by: myers
mutable description: 3L.3_23.A2.13 is 747 bases long and is cloned into the pGem-T vector. 3L.3_23.A2.13 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.A2.13 is a constituent of 3L.3_23.A2 (along with 3L.3_23.A2.01, 3L.3_23.A2.02, 3L.3_23.A2.03, 3L.3_23.A2.04, 3L.3_23.A2.05, 3L.3_23.A2.06, 3L.3_23.A2.07, 3L.3_23.A2.08, 3L.3_23.A2.09, 3L.3_23.A2.10, 3L.3_23.A2.11, 3L.3_23.A2.12, and 3L.3_23.A2.14.) This part contains at least part of the following features (positions offset from first base of sequence): kind and name offset notes gene YCL055W (410..+1417) Transcription factor required for induction of KAR3 and CIK1 during mating%2C also required during meiosis%3B exists in two forms%2C a slower-migrating form more abundant during vegetative growth and a faster-migrating form induced by pheromone forward_primer YCL055W_tagf1v1 (682..709) Sequence (the first 747 bases correspond to coordinates 17689..18435 in synthetic chromosome yeast_chr3_3_23)
discontinued: false
dominant: false
group u list: _385_
m user id: 0
owner id: 2669
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Constructed using overlap extension PCR protocol described in RFC38
star: false
creator: James DiCarlo
type: DnaRegion
role: SO:0000110
role: partType/Other
sequence: https://synbiohub.org/public/igem/BBa_K277025_sequence/1

BBa_K277026
type: ComponentDefinition
persistent identity: BBa K277026
display id: BBa_K277026
version: 1
was generated by: iGEM to SBOL conversion
description: 3L.3_23.A2.14
created: 2009-10-19T11:00:00Z
modified: 2015-05-08T01:11:46Z
mutable provenance: Synthetic Yeast
owned by: james
owned by: myers
mutable description: 3L.3_23.A2.14 is 750 bases long and is cloned into the pGem-T vector. 3L.3_23.A2.14 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.A2.14 is a constituent of 3L.3_23.A2 (along with 3L.3_23.A2.01, 3L.3_23.A2.02, 3L.3_23.A2.03, 3L.3_23.A2.04, 3L.3_23.A2.05, 3L.3_23.A2.06, 3L.3_23.A2.07, 3L.3_23.A2.08, 3L.3_23.A2.09, 3L.3_23.A2.10, 3L.3_23.A2.11, 3L.3_23.A2.12, and 3L.3_23.A2.13.) This part contains at least part of the following features (positions offset from first base of sequence): kind and name offset notes reverse_primer YCL055W_tagr1v1 (402..429) loxP_site loxPsym_YCL055W (685..718) gene YCL055W (-326..681) Transcription factor required for induction of KAR3 and CIK1 during mating%2C also required during meiosis%3B exists in two forms%2C a slower-migrating form more abundant during vegetative growth and a faster-migrating form induced by pheromone Sequence (the first 750 bases correspond to coordinates 18425..19174 in synthetic chromosome yeast_chr3_3_23)
discontinued: false
dominant: false
group u list: _385_
m user id: 0
owner id: 2669
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Constructed using overlap extension PCR protocol described in RFC38
star: false
creator: James DiCarlo
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K277026_sequence/1

https://synbiohub.org/public/igem/BBa_K277135_sequence/1
type: Sequence
persistent identity: BBa K277135 sequence
display id: BBa_K277135_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cagcttctggagttcaatcaacttcttcggaaaagataaaaaaataacttcgtataatgtacattatacgaagttatccattaattatcaaagctatcttgtccgctttcaaaaagtttattcgattttgttttcgttttcattttcatttttttattatgatggtcatggtggtagcggtggctcccttcagttttcctctttggtgctattaattttctcgttttccccatatatgtgatagaagcttttgaactccccactgtcttgtaagactttagaatcttgactgtcttgttaccttctttaaacttatcatttatgtctgttctggacgcaaaggatttgattattgatggatgtttgaaaacgccaactagtgatgtatctccattttccacctcatcttcatcctctgggcgcttttctaagtcaattattgtgtttactgtttttgaagtggtcgaatcagtttgagaatttgtgaatgactttatcgaactattctgttttagggtatataaatcttcaattgcttcatcattgccatgttgtatccttgatagctccttatccgtttcaaaatcctcataattattactttttaggaaggataagcttttctgcacaaagtcttcagatatgataacctttttgttcttttgatctacaggtttctgttcattatttcccgtattatcacctacattaatgctattgtcctgagtatctaggtcagtagccgtagtagccgtacttgtaatgtctaggttatattcctcttcagccccgaaggggtttttatattcgataatatcttccaccatactttcaaaaaatgcgctcgactttgggtttttaacaagctttgcatcatcgccaatttctaatctcctttttctcatcaattctctccttttgagacgatattgttgtagtacgtcgtcttcatcatcatcacttagttctagctctaagctattttttgctcttttattacgaaatcctccatttttaatgtcataaagaattttgtttatcattttgatatccatttccttattttctgcggcaagcatttctctgatttcatgaggattgaaattgttcttggaataatcatcgatcattttttctaggtcagaatcatagtcgtcagatccttctccatctgcaccaccgataccatgccattcatcctcggattcttcggcttccatttcaaagaagtttgtgacacctcttttcttcaactcttttatcttagcctcgtgctccttttctttctgttttagttgcaatttctctttgtcaatcagctgacggcgaattgcttcatcagcttcttcattgacgtatacatcatctttgacttcattttcgattgtatttctttcggccatcacctcttcaatgttttgtgtatctagaacattggtttcttgaaatttgtcggtatcctctccgatattatcaccgtaatggcccagattaatggcgataatggcgatccttttcggtagactttcatcagctttctctttgggtttggcttcgacttcggtatcagagtcactttcgttaatgatatgtttcacgtggtgggattttttttgctttagaataatttcatcatcttccttttcactatcggattctctacgattgtcattttcactaccagatgattcataatcggcaatttcattaccactcaatgcaaatccagaagactctgaccctgagtcagaaccggaatcatgggcgttcaattgaaagtcattttcttcaagtttttcccttctcttctctttttgtctgattcttttatttctaagaatttcttgttctagcaagttctctactatttccttttctttggccatatcctccagcttcagacctttcgtttcaatcacttctttctgatgatccagaatttgtttcctgctagctttccttaaggtatttaatagtacattatgatcaacttttgggtc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K277013_sequence/1
type: Sequence
persistent identity: BBa K277013 sequence
display id: BBa_K277013_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cagcttctggagttcaatcaacttcttcggaaaagataaaaaaataacttcgtataatgtacattatacgaagttatccattaattatcaaagctatcttgtccgctttcaaaaagtttattcgattttgttttcgttttcattttcatttttttattatgatggtcatggtggtagcggtggctcccttcagttttcctctttggtgctattaattttctcgttttccccatatatgtgatagaagcttttgaactccccactgtcttgtaagactttagaatcttgactgtcttgttaccttctttaaacttatcatttatgtctgttctggacgcaaaggatttgattattgatggatgtttgaaaacgccaactagtgatgtatctccattttccacctcatcttcatcctctgggcgcttttctaagtcaattattgtgtttactgtttttgaagtggtcgaatcagtttgagaatttgtgaatgactttatcgaactattctgttttagggtatataaatcttcaattgcttcatcattgccatgttgtatccttgatagctccttatccgtttcaaaatcctcataattattactttttaggaaggataagcttttctgcacaaagtcttcagatatgataacctttttgttcttttgatctacaggtttctgttcattatttcccgtattatcacctacattaatgctattgtcctgagtatctaggtcagtagccgt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K277014_sequence/1
type: Sequence
persistent identity: BBa K277014 sequence
display id: BBa_K277014_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agtagccgtacttgtaatgtctaggttatattcctcttcagccccgaaggggtttttatattcgataatatcttccaccatactttcaaaaaatgcgctcgactttgggtttttaacaagctttgcatcatcgccaatttctaatctcctttttctcatcaattctctccttttgagacgatattgttgtagtacgtcgtcttcatcatcatcacttagttctagctctaagctattttttgctcttttattacgaaatcctccatttttaatgtcataaagaattttgtttatcattttgatatccatttccttattttctgcggcaagcatttctctgatttcatgaggattgaaattgttcttggaataatcatcgatcattttttctaggtcagaatcatagtcgtcagatccttctccatctgcaccaccgataccatgccattcatcctcggattcttcggcttccatttcaaagaagtttgtgacacctcttttcttcaactcttttatcttagcctcgtgctccttttctttctgttttagttgcaatttctctttgtcaatcagctgacggcgaattgcttcatcagcttcttcattgacgtatacatcatctttgacttcattttcgattgtatttctttcggccatcacctcttcaatgttttgtgtatctagaacattggtttcttgaaatttgtcggtatcctctccgatattatcaccgtaatggcccagattaatggcgat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K277015_sequence/1
type: Sequence
persistent identity: BBa K277015 sequence
display id: BBa_K277015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aatggcgatccttttcggtagactttcatcagctttctctttgggtttggcttcgacttcggtatcagagtcactttcgttaatgatatgtttcacgtggtgggattttttttgctttagaataatttcatcatcttccttttcactatcggattctctacgattgtcattttcactaccagatgattcataatcggcaatttcattaccactcaatgcaaatccagaagactctgaccctgagtcagaaccggaatcatgggcgttcaattgaaagtcattttcttcaagtttttcccttctcttctctttttgtctgattcttttatttctaagaatttcttgttctagcaagttctctactatttccttttctttggccatatcctccagcttcagacctttcgtttcaatcacttctttctgatgatccagaatttgtttcctgctagctttccttaaggtatttaatagtacattatgatcaacttttgggtctttacttttattcggcctttgagacaacttttgattttgtttagagagtcttgccttcaggtttaatatagtcgcctttgataactgagagattggcaaaacgctttcatctttggataatttaatgctatccatatcatcatctccatattcgtcactgtcgctgtcgaggtccagtgttatgcatttcgaggagtcaatttcccgtttcaaattattagcatagct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K277016_sequence/1
type: Sequence
persistent identity: BBa K277016 sequence
display id: BBa_K277016_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agcatagctagatagcaaagggactcgcttttcgtttattcttgtctcatcagttagatttaattcaacacttttgttctcatgcagttcatcgtcattctgtactggttttggatgtgcattttccagctttatgtcgtcatcttcgttgctgctgctgtcatcaaaatcagcaagaaaagactctttagaaaaaaattttttggttggtattggcttttgcggttttttgtattcgacatttctagctttttcccttttagaatcttctgaagccaattcgctttgtatttcccgaatttttaatgtgtgtggtacttcatcttgaagtgtttgtgcgactgtatctatttgggttttctgttcctgatgcgtagcctgtggaataccaattgcttgggttttgtcttcgtcgtgagcggtttgaggtggtacatccatttgggtgtcaggaccacgattttgaatggactgaaataaaggactagttccaataagttccgttggaattataggaattttcggcgacagagcattagaatgattgctggaagtcgtaattatttgggattgtggttcaattgattttataggctgagtttgtgaagtagcgccagaagagtcgattcgttgggtagcccctggccgtcctgtcgtgagaggtgttttcagcgcttgagaagtttcgttgacgagaccatcttctttgattaattgggtgggtttcccgtcattaacggaat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K277017_sequence/1
type: Sequence
persistent identity: BBa K277017 sequence
display id: BBa_K277017_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acggaatggataggtacgttaaacaccttgtcttgttggatgctgacaggtattcgttgggtttgggtaaagctgctgctaacatttttctcactgtaactattatctttaacttcggatttctccagctcttcaccaccatcgtaaagattagagattaattgagttggtaacgaattctcactcctatcttttccattattgtgattctgttcgggtgctttcttgccttctaatctgttcttaacacgatttaatgtggcattggcaaataaaaacccattaccggttagttcaggtgggttatcgatgtcattgggcccatttccattagtattatcgttctcatctagtatgggaacagcaacttttttgtaggtggtagttctcttctttgcagtcaacgaggacaaagcatgcaaggcatcatccatcactaaaatatttggtgataagttcaaaagcgaataacgaacttccttagttgtttgtctgttcttggtttttttccaacataatgtgtttgaaatgctattgaagcaaacgcgttttacgcattctatctcttcatgacgcgtcgaagaaggataaggaaaatttcaagtattgatgtatatctctctgcatatgagagccagtagagttggtgtcctagtaagaatttctttcactttacaaacatatctaagtaaacgaaatgtgtgtgtgtttctatatatatatagacatatatgaaggatt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K277018_sequence/1
type: Sequence
persistent identity: BBa K277018 sequence
display id: BBa_K277018_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaggattccgtagcggtgtaaattaattttggtttggcttgtatgcttcttcttccggagctatgaaatcttttgctctctcttcctgcctttcgatttctctttccatttgcttttccttttgctcatttaatttcttcatttgcttttctctcttgcttaagaaatactcaccactttcaatttccaagtcgacttttcgtggtagttgagctggtggaaatggagtatagaccttcttttcgacgtttctgatcttcttgggtttctttctggccacattcctcttcttaaacatgggcaagaatcttgaccaatcttcattggctaactctggcctttttgccaattctctttttatcattaattccttgatatgatagatagggtgaatatttttcatacaatcttctactactcgacggacctccttcaagcccttgaagggacccatggcacttactgtgttaccttgtactagaatgtaacatttagttagaagttccaaagcctttaaagtattaccgttagggcctacaagacgttgtcttctcttgacaaacctttctttgttagtaacgaaattaccaattttaataacgtcgcatgccatgtcatcttgtaggatcttaacggcttgtggaaatgggacgcttcgagctagcaatttgatcaaatctctggctttcaaaatgatagcgggatcgtatgtttttctagttgtttttactgtcatagaaccttcgact
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K277019_sequence/1
type: Sequence
persistent identity: BBa K277019 sequence
display id: BBa_K277019_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaccttcgactaaatctagaacacacgctatgttgtgtttgtctagagcccttgttacatcattccaaatcgtcttcaagtaactttctctgtatttaggaaacaaagtcataaaactggactcttcagcaaaaggttgaccggatgcgttatcctcttccttaaactcctctatcttccatttatcaatatcatccgtatcccaaggtttatctctgttatgtgtagacaccatcgtttgccaatttggatatttgtgtgacccttttgtttgctgtctactttacaatagttaacctcatcatctcttttttttgaaaattttcatatctcatcgctaaaagaattagaaatataaggaaaaaaaaattttcgttttcagatgtgcaagcctgctataataaggtacaataactcaagggcatttagcaaggaaaaaatgggcaagtgtagcatgaaaaagaaaggtgtgggcaagaatgttggtgttggcaagaaagtacaaaaaaagaggtcgatcagcaccgctgaaaggaagagaacaaagttacaagtggaaaagttaaacaaaagtagtgaaacaatgataccgacgctgctgcgggaggcaagtacacaagagccagctaaactgaaagctgagactactttgaaagccgaggagctgatcaaggaccaggaaaaggactccaaggtacgagagcaaattcggacagaaaaatcaaaaacaaacgacagcatgctgaagcagatcgaaatgatatccggcttttccttataaggaatagtggtgaaagttacgtaaatatatacat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K277020_sequence/1
type: Sequence
persistent identity: BBa K277020 sequence
display id: BBa_K277020_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aatatatacatatagagcggaatggtactgcatatttttattatttttttttttgaatatatataaagcatcgtcgcttaagattaaaccttcgaggatctgaggccagcgctggatctaaagatggcgtcgccctcggcgtagcctcttccaacaccaaatccaatgcctagccatacaggaaatgcacggcgcttgaagaataatactgaggtgaacacacccacgccaaaacccatggcagtcttgaccagcatgttggacaggacaatatcccacttagtgtcgaggatcgtggacacggagctgccattcttgttagaatccttggagggagtgcttttcgctggttgttgtgtttgtgcttgttcggacatggtatattttgtctttttccgtgtttattccctctcgtagcaaagttaatctgccaattgacagtagtttaatatatggtattatcgttgtttgaagccctttaaaaggaaaaaacgggttagggccacccggcgcgaagtaatagctgctgattggtcagaatagtaaaacggcttgaattgatagtatatcgtactgtatataccttatatttgggctaataatcatctcccaaatgctccagtatacatgtcacctagctatgtatgaggttgactaccttatatgcagctact
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K277021_sequence/1
type: Sequence
persistent identity: BBa K277021 sequence
display id: BBa_K277021_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agctactacacaataactgtagtatcgcgctgcacgagcgtgacggcggttaaatctggattttttgccaccttttttatacgaaaataagaactaggtaaataggaaggaatggttggatttgagtttaaaaatagagggaatacttggagatgcgattgttgctgtgcaagaattggtttgcgtcacctgtaatctcaccactactgtatacccgctccttatattcaatggctaacactactagtttccctattgctccccaggccccgcctaactggagctttaccccatctgacatctctgggaaaaccaacgaaatcatcaacaacagcaacaatttctatgattctatgagtaaggtagagagcccttccgtgagtaattttgtggagcctttcatgaagtttgaaaatgaattgggcccaataattaaccaattaactttcttacagcatgtgtcgtctgataaggaaatccgagatgctagcgttaatagctcaatgaaactggatgagttgaacatcgatctatctctgcgtcacgacatctttttgcaattcgcccgcgtctggcaggatgttcaatcgaaggcggattctgtggaaagagaaactttcaaatacgttgagaagtcttacaaggactacattcattctggtttggaacttgacgagggaaaccgattgaaaatcaaagagatcaaaaagaagatctccgttaactctattaatttttcgaagaatctgggagaacaaaaggaatacatcactttcaccaaagaacaattggaaggtgtgccggattctattttgacgcagttcgagacaataaaatct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K277022_sequence/1
type: Sequence
persistent identity: BBa K277022 sequence
display id: BBa_K277022_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ataaaatctgacaaagatagcaatgaaaccttgtataaagtcaccttcaaatatccggacatttttcccgtgatgaaattggcatcctcagctcagactagaaagcaggcctttttggccgaccaaaataaggtccctgaaaatgaagctatactgttggatacattgaagctgcgtgacgaattggcctcgttattgggctatgacacgtatgcgaactacaacctgtatgataaaatggctgaagatagcactacggtaatgaactttttgaatgatttgaaggacaagctaattccgctgggcagaaaggaactacaggtcttgcaagatatgaaagccgaagatgttaagaaacttaaccagggtgcagatccaaactactacatttgggaccaccgttactacgataacaaatatttgttagaaaacttcaatgtggacctagaaaagatttctgaatattttcctttggaagccaccatcaccggcatgttggaaatatacgaaacattgtttaatttgaagtttatcgagacgaaagattctcaaaacaaatctgtttggcatgacgacgtcaaacaaatcgccgtttggaatatggatgatccaaagtctccaaactttgttggttggatttatttcgatttacatcctcgtgatggtaaatatggccacgctgccaattttggtttatcgtcatcattcatgattgatgacaccacaagatcgtatccggttactgcgt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K277023_sequence/1
type: Sequence
persistent identity: BBa K277023 sequence
display id: BBa_K277023_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: actgcgttggtttgcaatttctccaaatctacgaaggataaaccttctctactgaagcataacgaaatagtgacctttttccatgaattgggccatggtatccatgacctggtgggacaaaacaaagaaagccgattcaacggtccaggtagcgttccatgggattttgtggaggcaccttcccaaatgttagaattttggacttggaataagaatgaattaatcaacctctcatcacattacaaaacgggcgaaaaaattccagaatctttgatcaattcattgatcaaaactaaacacgtaaatggtgctttattcactctaagacaattacattttgggttatttgatatgaaagtacatacttgtaaagacttgcaaaacctgtcaatttgcgatacctggaaccaattgagacaggatatttctttgatttctaatggtggtacgttatccaagggttatgattcatttggccatataatgtcagactcttactctgccggttattacggttatctatgggcggaagtctttgcaactgatatgtatcacaccaaattcgctaaggatccgttaaatgccaagaatgggatacaataccgtgatattgtgttggctcgtggtggcctttatgatattaat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K277024_sequence/1
type: Sequence
persistent identity: BBa K277024 sequence
display id: BBa_K277024_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgatattaatgataatctgaaagaatttttgggtagggaaccttctaaggatgctttcttgaaggagctgggcttacagaactaaattataacttcgtataatgtacattatacgaagttatcgttcaagcataaaattatacaattattctacaaagtgttattatgttagataatcttttaaataacctcgtccctggatagattaaaaccattaaatagctcaaatcttggagtaaagcagcagtaatatgtctgatatgttcgttatattttttcttgaagtttatcaacttatcctttactagaccatttccctccccatccaatcgttgatcgattttaaatttcaccttgcataattttattaatttccatacacattttatcactgaaatgcacgagtaacacagccaaactttccgtaatagcccacttacatataacttatcgttgaccacgcctaggttttccaatactgcgaagaaatcaagcagcgaacttacaccactccatatattctcccatatatcttttgcactgatttcagccgtatttttcttcgaaaccattcaactttcttttcttcttcctttacttttattcatctcctctttacttcttacctcagagtcgaacattgaaatcatttcccgcgaatttttcgattataattaccctttctacttaacaagaagcaataagagcaaaggggcgaaatgcttgtcccatactttggct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K277025_sequence/1
type: Sequence
persistent identity: BBa K277025 sequence
display id: BBa_K277025_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: actttggcttttggatgtgacattaggagccttatctcctcttaatgccttaagtcaaaaaatagtttttgggatatatcttgtgatcaaatgctttgcaaaagggtaaaacagcgtttacaataggatagccaaaagtaaaaaaaaaaaaaaaaaaaaaaagaaggaagttagtatcgagctcaaacaattttagttttgctggacatattttcagtttcattactgtatatttgcagcttggtttttgtcgctctgtagtcttgtttcacacttcgtcatttccgagtttcatcttgcgacataacttgtttcaacttttcaagtgcgactttttgttaaatattatgacatagaagaaagtagaacagccattataaaacaaaagagaagtgagaatactattaagatggcattccaagatccaacttacgaccagaataaaagcagacacatcaacaacagtcacttgcaagggccaaaccaggaaacaatagaaatgaaatctaaacacgtatcattcaaaccctctagagacttccatacaaacgattactcgaataactacattcatgggaagtcgctaccgcaacagcatgttactaatattgagaatagggttgatggctatccaaaacttcagaaattatttcaggcgaaagctaaacaaataaatcaattcgctaccacccctttcggttgcaagattggaatagattccattgttccaacgttgaatcactggat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K277026_sequence/1
type: Sequence
persistent identity: BBa K277026 sequence
display id: BBa_K277026_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aatcactggatacagaacgaaaatttgactttcgacgtggtgatgattggctgcttaacagaaaatcagtttatttacccaattttaacccaattgccattggatagattgatctccaaaccaggtttcctgttcatctgggccaattctcaaaaaatcaatgaacttactaaacttttgaataatgaaatatgggctaaaaagtttagaagaagtgaagaattggtttttgttcctattgacaagaaatcaccgttttatccaggtttagatcaggacgatgaaacgttgatggaaaaaatgcaatggcactgttggatgtgtatcacaggtacagtaaggaggtctacagatggacatcttattcattgtaacgtagacactgacttgagtatcgaaaccaaagatactaccaacggcgccgttccttcccatttgtatcgtattgcagaaaacttctctaccgcgactagacgattacatattattcctgcaaggactggttacgagacacccgtcaaagtaagacctggctgggttatagtgagcccagatgttatgttggataacttctcacccaagagatataaagaagagatagctaatttaggttcgaatatcccattaaaaaatgagattgagctgttaagaccaagaagtccagtacaaaaagcacaataaagcataacttcgtataatgtacattatacgaagttatatcaacacataaatccttgcttagctcaatta
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z