BBa_K277136
type: ComponentDefinition
persistent identity: BBa K277136
display id: BBa_K277136
version: 1
was generated by: iGEM to SBOL conversion
description: 3L.3_23.B1
created: 2009-10-20T11:00:00Z
modified: 2015-05-08T01:11:47Z
mutable provenance: Synthetic Yeast
owned by: james
owned by: myers
mutable description: 3L.3_23.B1 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns,repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.B1 is comprised of and must be constructed from the parts 3L.3_23.B1.01, 3L.3_23.B1.02, 3L.3_23.B1.03, 3L.3_23.B1.04, 3L.3_23.B1.05, 3L.3_23.B1.06, 3L.3_23.B1.07, 3L.3_23.B1.08, 3L.3_23.B1.09, 3L.3_23.B1.10, 3L.3_23.B1.11, 3L.3_23.B1.12, 3L.3_23.B1.13, and 3L.3_23.B1.14. This part wholly contains the following features (positions offset from first base of sequence): kind and name offset notes reverse_primer YCL054W_tagr3v1 (4762..4789) gene YCL050C (8947..9912) Diadenosine 5'%2C5''-P1%2CP4-tetraphosphate phosphorylase I (AP4A phosphorylase)%2C involved in catabolism of bis(5'-nucleosidyl) tetraphosphates%3B has similarity to Apa2p mutation_affecting_coding_sequence YCL054W_re_remove_Bpu10I (4706..4714) removal of Bpu10I loxP_site loxPsym_YCL051W (8663..8696) reverse_primer YCL054W_tagr1v1 (4321..4348) forward_primer YCL051W_tagf1v1 (8263..8290) reverse_primer YCL050C_tagr1v1 (9442..9469) mutation_affecting_coding_sequence YCL054W_re_remove_MmeI (3008..3019) removal of MmeI mutation_affecting_coding_sequence YCL054W_re_remove_BlpI (3947..3955) removal of BlpI mutation_affecting_coding_sequence YCL051W_re_remove_MmeI (7637..7648) removal of MmeI ARS ARS304 (1243..1700) Autonomously Replicating Sequence on Chromosome III gene YCL051W (6908..8659) Protein involved in control of cell wall structure and stress response%3B inhibits Cbk1p protein kinase activity%3B overproduction confers re…
discontinued: false
dominant: false
group u list: _385_
m user id: 0
owner id: 2669
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Constructed using USER assembly method described in RFC38
star: false
creator: James DiCarlo
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K277038
component: BBa_K277037
component: BBa_K277040
component: BBa_K277039
component: BBa_K277034
component: BBa_K277033
component: BBa_K277036
component: BBa_K277035
component: BBa_K277030
component: BBa_K277029
component: BBa_K277032
component: BBa_K277031
component: BBa_K277028
component: BBa_K277027
sequence annotation: BBa_K277027
sequence annotation: BBa_K277028
sequence annotation: BBa_K277029
sequence annotation: BBa_K277030
sequence annotation: BBa_K277031
sequence annotation: BBa_K277032
sequence annotation: BBa_K277033
sequence annotation: BBa_K277034
sequence annotation: BBa_K277035
sequence annotation: BBa_K277036
sequence annotation: BBa_K277037
sequence annotation: BBa_K277038
sequence annotation: BBa_K277039
sequence annotation: BBa_K277040
sequence: https://synbiohub.org/public/igem/BBa_K277136_sequence/1

BBa_K277038
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BBa_K277037
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display id: component2062015
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BBa_K277034
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BBa_K277035
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BBa_K277030
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BBa_K277032
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BBa_K277031
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BBa_K277028
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BBa_K277027
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BBa_K277027
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BBa_K277029
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BBa_K277031
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persistent identity: annotation2062009
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BBa_K277032
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BBa_K277033
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BBa_K277034
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persistent identity: annotation2062012
display id: annotation2062012
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location: https://synbiohub.org/public/igem/BBa_K277136/annotation2062012/range2062012/1
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BBa_K277035
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BBa_K277036
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BBa_K277037
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BBa_K277038
type: SequenceAnnotation
persistent identity: annotation2062016
display id: annotation2062016
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BBa_K277039
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persistent identity: annotation2062017
display id: annotation2062017
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persistent identity: range2062017
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BBa_K277040
type: SequenceAnnotation
persistent identity: annotation2062018
display id: annotation2062018
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type: Range
persistent identity: range2062018
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BBa_K277027
type: ComponentDefinition
persistent identity: BBa K277027
display id: BBa_K277027
version: 1
was generated by: iGEM to SBOL conversion
description: 3L.3_23.B1.01
created: 2009-10-19T11:00:00Z
modified: 2015-05-08T01:11:46Z
mutable provenance: Synthetic Yeast
owned by: james
owned by: myers
mutable description: 3L.3_23.B1.01 is 744 bases long and is cloned into the pGem-T vector. 3L.3_23.B1.01 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.B1.01 is a constituent of 3L.3_23.B1 (along with 3L.3_23.B1.02, 3L.3_23.B1.03, 3L.3_23.B1.04, 3L.3_23.B1.05, 3L.3_23.B1.06, 3L.3_23.B1.07, 3L.3_23.B1.08, 3L.3_23.B1.09, 3L.3_23.B1.10, 3L.3_23.B1.11, 3L.3_23.B1.12, 3L.3_23.B1.13, and 3L.3_23.B1.14.) This part contains at least part of the following features (positions offset from first base of sequence): kind and name offset notes Sequence (the first 744 bases correspond to coordinates 19161..19904 in synthetic chromosome yeast_chr3_3_23)
discontinued: false
dominant: false
group u list: _385_
m user id: 0
owner id: 2669
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Constructed using overlap extension PCR protocol described in RFC38
star: false
creator: James DiCarlo
type: DnaRegion
role: SO:0000110
role: partType/Other
sequence: https://synbiohub.org/public/igem/BBa_K277027_sequence/1

BBa_K277028
type: ComponentDefinition
persistent identity: BBa K277028
display id: BBa_K277028
version: 1
was generated by: iGEM to SBOL conversion
description: 3L.3_23.B1.02
created: 2009-10-19T11:00:00Z
modified: 2015-05-08T01:11:46Z
mutable provenance: Synthetic Yeast
owned by: james
owned by: myers
mutable description: 3L.3_23.B1.02 is 763 bases long and is cloned into the pGem-T vector. 3L.3_23.B1.02 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.B1.02 is a constituent of 3L.3_23.B1 (along with 3L.3_23.B1.01, 3L.3_23.B1.03, 3L.3_23.B1.04, 3L.3_23.B1.05, 3L.3_23.B1.06, 3L.3_23.B1.07, 3L.3_23.B1.08, 3L.3_23.B1.09, 3L.3_23.B1.10, 3L.3_23.B1.11, 3L.3_23.B1.12, 3L.3_23.B1.13, and 3L.3_23.B1.14.) This part contains at least part of the following features (positions offset from first base of sequence): kind and name offset notes ARS ARS304 (508..+965) Autonomously Replicating Sequence on Chromosome III Sequence (the first 763 bases correspond to coordinates 19896..20658 in synthetic chromosome yeast_chr3_3_23)
discontinued: false
dominant: false
group u list: _385_
m user id: 0
owner id: 2669
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Constructed using overlap extension PCR protocol described in RFC38
star: false
creator: James DiCarlo
type: DnaRegion
role: SO:0000110
role: partType/Other
sequence: https://synbiohub.org/public/igem/BBa_K277028_sequence/1

BBa_K277029
type: ComponentDefinition
persistent identity: BBa K277029
display id: BBa_K277029
version: 1
was generated by: iGEM to SBOL conversion
description: 3L.3_23.B1.03
created: 2009-10-19T11:00:00Z
modified: 2015-05-08T01:11:46Z
mutable provenance: Synthetic Yeast
owned by: james
owned by: myers
mutable description: 3L.3_23.B1.03 is 756 bases long and is cloned into the pGem-T vector. 3L.3_23.B1.03 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.B1.03 is a constituent of 3L.3_23.B1 (along with 3L.3_23.B1.01, 3L.3_23.B1.02, 3L.3_23.B1.04, 3L.3_23.B1.05, 3L.3_23.B1.06, 3L.3_23.B1.07, 3L.3_23.B1.08, 3L.3_23.B1.09, 3L.3_23.B1.10, 3L.3_23.B1.11, 3L.3_23.B1.12, 3L.3_23.B1.13, and 3L.3_23.B1.14.) This part contains at least part of the following features (positions offset from first base of sequence): kind and name offset notes ARS ARS304 (-246..211) Autonomously Replicating Sequence on Chromosome III Sequence (the first 756 bases correspond to coordinates 20650..21405 in synthetic chromosome yeast_chr3_3_23)
discontinued: false
dominant: false
group u list: _385_
m user id: 0
owner id: 2669
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Constructed using overlap extension PCR protocol described in RFC38
star: false
creator: James DiCarlo
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K277029_sequence/1

BBa_K277030
type: ComponentDefinition
persistent identity: BBa K277030
display id: BBa_K277030
version: 1
was generated by: iGEM to SBOL conversion
description: 3L.3_23.B1.04
created: 2009-10-19T11:00:00Z
modified: 2015-05-08T01:11:46Z
mutable provenance: Synthetic Yeast
owned by: james
owned by: myers
mutable description: 3L.3_23.B1.04 is 753 bases long and is cloned into the pGem-T vector. 3L.3_23.B1.04 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.B1.04 is a constituent of 3L.3_23.B1 (along with 3L.3_23.B1.01, 3L.3_23.B1.02, 3L.3_23.B1.03, 3L.3_23.B1.05, 3L.3_23.B1.06, 3L.3_23.B1.07, 3L.3_23.B1.08, 3L.3_23.B1.09, 3L.3_23.B1.10, 3L.3_23.B1.11, 3L.3_23.B1.12, 3L.3_23.B1.13, and 3L.3_23.B1.14.) This part contains at least part of the following features (positions offset from first base of sequence): kind and name offset notes forward_primer YCL054W_tagf2v1 (446..473) gene YCL054W (258..+2783) AdoMet-dependent methyltransferase involved in rRNA processing and 60S ribosomal subunit maturation%3B methylates G2922 in the tRNA docking site of the large subunit rRNA and in the absence of snR52%2C U2921%3B suppressor of PAB1 mutants Sequence (the first 753 bases correspond to coordinates 21395..22147 in synthetic chromosome yeast_chr3_3_23)
discontinued: false
dominant: false
group u list: _385_
m user id: 0
owner id: 2669
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Constructed using overlap extension PCR protocol described in RFC38
star: false
creator: James DiCarlo
type: DnaRegion
role: SO:0000110
role: partType/Other
sequence: https://synbiohub.org/public/igem/BBa_K277030_sequence/1

BBa_K277031
type: ComponentDefinition
persistent identity: BBa K277031
display id: BBa_K277031
version: 1
was generated by: iGEM to SBOL conversion
description: 3L.3_23.B1.05
created: 2009-10-19T11:00:00Z
modified: 2015-05-08T01:11:46Z
mutable provenance: Synthetic Yeast
owned by: james
owned by: myers
mutable description: 3L.3_23.B1.05 is 706 bases long and is cloned into the pGem-T vector. 3L.3_23.B1.05 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.B1.05 is a constituent of 3L.3_23.B1 (along with 3L.3_23.B1.01, 3L.3_23.B1.02, 3L.3_23.B1.03, 3L.3_23.B1.04, 3L.3_23.B1.06, 3L.3_23.B1.07, 3L.3_23.B1.08, 3L.3_23.B1.09, 3L.3_23.B1.10, 3L.3_23.B1.11, 3L.3_23.B1.12, 3L.3_23.B1.13, and 3L.3_23.B1.14.) This part contains at least part of the following features (positions offset from first base of sequence): kind and name offset notes mutation_affecting_coding_sequence YCL054W_re_remove_MmeI (32..43) removal of MmeI reverse_primer YCL054W_tagr2v1 (124..151) gene YCL054W (-484..+2041) AdoMet-dependent methyltransferase involved in rRNA processing and 60S ribosomal subunit maturation%3B methylates G2922 in the tRNA docking site of the large subunit rRNA and in the absence of snR52%2C U2921%3B suppressor of PAB1 mutants Sequence (the first 706 bases correspond to coordinates 22137..22842 in synthetic chromosome yeast_chr3_3_23)
discontinued: false
dominant: false
group u list: _385_
m user id: 0
owner id: 2669
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Constructed using overlap extension PCR protocol described in RFC38
star: false
creator: James DiCarlo
type: DnaRegion
role: SO:0000110
role: partType/Other
sequence: https://synbiohub.org/public/igem/BBa_K277031_sequence/1

BBa_K277032
type: ComponentDefinition
persistent identity: BBa K277032
display id: BBa_K277032
version: 1
was generated by: iGEM to SBOL conversion
description: 3L.3_23.B1.06
created: 2009-10-19T11:00:00Z
modified: 2015-05-08T01:11:46Z
mutable provenance: Synthetic Yeast
owned by: james
owned by: myers
mutable description: 3L.3_23.B1.06 is 750 bases long and is cloned into the pGem-T vector. 3L.3_23.B1.06 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.B1.06 is a constituent of 3L.3_23.B1 (along with 3L.3_23.B1.01, 3L.3_23.B1.02, 3L.3_23.B1.03, 3L.3_23.B1.04, 3L.3_23.B1.05, 3L.3_23.B1.07, 3L.3_23.B1.08, 3L.3_23.B1.09, 3L.3_23.B1.10, 3L.3_23.B1.11, 3L.3_23.B1.12, 3L.3_23.B1.13, and 3L.3_23.B1.14.) This part contains at least part of the following features (positions offset from first base of sequence): kind and name offset notes reverse_primer YCL054W_tagr1v1 (648..675) mutation_affecting_coding_sequence YCL054W_re_remove_BlpI (274..282) removal of BlpI forward_primer YCL054W_tagf1v1 (243..270) gene YCL054W (-1181..+1344) AdoMet-dependent methyltransferase involved in rRNA processing and 60S ribosomal subunit maturation%3B methylates G2922 in the tRNA docking site of the large subunit rRNA and in the absence of snR52%2C U2921%3B suppressor of PAB1 mutants Sequence (the first 750 bases correspond to coordinates 22834..23583 in synthetic chromosome yeast_chr3_3_23)
discontinued: false
dominant: false
group u list: _385_
m user id: 0
owner id: 2669
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Constructed using overlap extension PCR protocol described in RFC38
star: false
creator: James DiCarlo
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K277032_sequence/1

BBa_K277033
type: ComponentDefinition
persistent identity: BBa K277033
display id: BBa_K277033
version: 1
was generated by: iGEM to SBOL conversion
description: 3L.3_23.B1.07
created: 2009-10-19T11:00:00Z
modified: 2015-05-08T01:11:46Z
mutable provenance: Synthetic Yeast
owned by: james
owned by: myers
mutable description: 3L.3_23.B1.07 is 750 bases long and is cloned into the pGem-T vector. 3L.3_23.B1.07 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.B1.07 is a constituent of 3L.3_23.B1 (along with 3L.3_23.B1.01, 3L.3_23.B1.02, 3L.3_23.B1.03, 3L.3_23.B1.04, 3L.3_23.B1.05, 3L.3_23.B1.06, 3L.3_23.B1.08, 3L.3_23.B1.09, 3L.3_23.B1.10, 3L.3_23.B1.11, 3L.3_23.B1.12, 3L.3_23.B1.13, and 3L.3_23.B1.14.) This part contains at least part of the following features (positions offset from first base of sequence): kind and name offset notes reverse_primer YCL054W_tagr3v1 (350..377) mutation_affecting_coding_sequence YCL054W_re_remove_Bpu10I (294..302) removal of Bpu10I forward_primer YCL054W_tagf3v1 (74..101) gene YCL054W (-1920..605) AdoMet-dependent methyltransferase involved in rRNA processing and 60S ribosomal subunit maturation%3B methylates G2922 in the tRNA docking site of the large subunit rRNA and in the absence of snR52%2C U2921%3B suppressor of PAB1 mutants Sequence (the first 750 bases correspond to coordinates 23573..24322 in synthetic chromosome yeast_chr3_3_23)
discontinued: false
dominant: false
group u list: _385_
m user id: 0
owner id: 2669
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Constructed using overlap extension PCR protocol described in RFC38
star: false
creator: James DiCarlo
type: DnaRegion
role: SO:0000110
role: partType/Other
sequence: https://synbiohub.org/public/igem/BBa_K277033_sequence/1

BBa_K277034
type: ComponentDefinition
persistent identity: BBa K277034
display id: BBa_K277034
version: 1
was generated by: iGEM to SBOL conversion
description: 3L.3_23.B1.08
created: 2009-10-19T11:00:00Z
modified: 2015-05-08T01:11:46Z
mutable provenance: Synthetic Yeast
owned by: james
owned by: myers
mutable description: 3L.3_23.B1.08 is 804 bases long and is cloned into the pGem-T vector. 3L.3_23.B1.08 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.B1.08 is a constituent of 3L.3_23.B1 (along with 3L.3_23.B1.01, 3L.3_23.B1.02, 3L.3_23.B1.03, 3L.3_23.B1.04, 3L.3_23.B1.05, 3L.3_23.B1.06, 3L.3_23.B1.07, 3L.3_23.B1.09, 3L.3_23.B1.10, 3L.3_23.B1.11, 3L.3_23.B1.12, 3L.3_23.B1.13, and 3L.3_23.B1.14.) This part contains at least part of the following features (positions offset from first base of sequence): kind and name offset notes forward_primer YCL052C_tagf1v1 (620..647) gene YCL052C (35..+1285) Essential component of glycosylphosphatidylinositol-mannosyltransferase I%2C required for the autocatalytic post-translational processing of the protease B precursor Prb1p%2C localizes to ER in lumenal orientation%3B homolog of mammalian PIG-X Sequence (the first 804 bases correspond to coordinates 24312..25115 in synthetic chromosome yeast_chr3_3_23)
discontinued: false
dominant: false
group u list: _385_
m user id: 0
owner id: 2669
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Constructed using overlap extension PCR protocol described in RFC38
star: false
creator: James DiCarlo
type: DnaRegion
role: SO:0000110
role: partType/Other
sequence: https://synbiohub.org/public/igem/BBa_K277034_sequence/1

BBa_K277035
type: ComponentDefinition
persistent identity: BBa K277035
display id: BBa_K277035
version: 1
was generated by: iGEM to SBOL conversion
description: 3L.3_23.B1.09
created: 2009-10-19T11:00:00Z
modified: 2015-05-08T01:11:46Z
mutable provenance: Synthetic Yeast
owned by: james
owned by: myers
mutable description: 3L.3_23.B1.09 is 754 bases long and is cloned into the pGem-T vector. 3L.3_23.B1.09 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.B1.09 is a constituent of 3L.3_23.B1 (along with 3L.3_23.B1.01, 3L.3_23.B1.02, 3L.3_23.B1.03, 3L.3_23.B1.04, 3L.3_23.B1.05, 3L.3_23.B1.06, 3L.3_23.B1.07, 3L.3_23.B1.08, 3L.3_23.B1.10, 3L.3_23.B1.11, 3L.3_23.B1.12, 3L.3_23.B1.13, and 3L.3_23.B1.14.) This part contains at least part of the following features (positions offset from first base of sequence): kind and name offset notes reverse_primer YCL052C_tagr1v1 (213..240) gene YCL052C (-762..488) Essential component of glycosylphosphatidylinositol-mannosyltransferase I%2C required for the autocatalytic post-translational processing of the protease B precursor Prb1p%2C localizes to ER in lumenal orientation%3B homolog of mammalian PIG-X Sequence (the first 754 bases correspond to coordinates 25109..25862 in synthetic chromosome yeast_chr3_3_23)
discontinued: false
dominant: false
group u list: _385_
m user id: 0
owner id: 2669
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Constructed using overlap extension PCR protocol described in RFC38
star: false
creator: James DiCarlo
type: DnaRegion
role: SO:0000110
role: partType/Other
sequence: https://synbiohub.org/public/igem/BBa_K277035_sequence/1

BBa_K277036
type: ComponentDefinition
persistent identity: BBa K277036
display id: BBa_K277036
version: 1
was generated by: iGEM to SBOL conversion
description: 3L.3_23.B1.10
created: 2009-10-20T11:00:00Z
modified: 2015-05-08T01:11:46Z
mutable provenance: Synthetic Yeast
owned by: james
owned by: myers
mutable description: 3L.3_23.B1.10 is 678 bases long and is cloned into the pGem-T vector. 3L.3_23.B1.10 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.B1.10 is a constituent of 3L.3_23.B1 (along with 3L.3_23.B1.01, 3L.3_23.B1.02, 3L.3_23.B1.03, 3L.3_23.B1.04, 3L.3_23.B1.05, 3L.3_23.B1.06, 3L.3_23.B1.07, 3L.3_23.B1.08, 3L.3_23.B1.09, 3L.3_23.B1.11, 3L.3_23.B1.12, 3L.3_23.B1.13, and 3L.3_23.B1.14.) This part contains at least part of the following features (positions offset from first base of sequence): kind and name offset notes gene YCL051W (217..+1968) Protein involved in control of cell wall structure and stress response%3B inhibits Cbk1p protein kinase activity%3B overproduction confers resistance to cell-wall degrading enzymes forward_primer YCL051W_tagf2v1 (630..657) Sequence (the first 678 bases correspond to coordinates 25852..26529 in synthetic chromosome yeast_chr3_3_23)
discontinued: false
dominant: false
group u list: _385_
m user id: 0
owner id: 2669
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Constructed using overlap extension PCR protocol described in RFC38
star: false
creator: James DiCarlo
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K277036_sequence/1

BBa_K277037
type: ComponentDefinition
persistent identity: BBa K277037
display id: BBa_K277037
version: 1
was generated by: iGEM to SBOL conversion
description: 3L.3_23.B1.11
created: 2009-10-20T11:00:00Z
modified: 2015-05-08T01:11:46Z
mutable provenance: Synthetic Yeast
owned by: james
owned by: myers
mutable description: 3L.3_23.B1.11 is 745 bases long and is cloned into the pGem-T vector. 3L.3_23.B1.11 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.B1.11 is a constituent of 3L.3_23.B1 (along with 3L.3_23.B1.01, 3L.3_23.B1.02, 3L.3_23.B1.03, 3L.3_23.B1.04, 3L.3_23.B1.05, 3L.3_23.B1.06, 3L.3_23.B1.07, 3L.3_23.B1.08, 3L.3_23.B1.09, 3L.3_23.B1.10, 3L.3_23.B1.12, 3L.3_23.B1.13, and 3L.3_23.B1.14.) This part contains at least part of the following features (positions offset from first base of sequence): kind and name offset notes mutation_affecting_coding_sequence YCL051W_re_remove_MmeI (277..288) removal of MmeI gene YCL051W (-452..+1299) Protein involved in control of cell wall structure and stress response%3B inhibits Cbk1p protein kinase activity%3B overproduction confers resistance to cell-wall degrading enzymes reverse_primer YCL051W_tagr2v1 (429..456) Sequence (the first 745 bases correspond to coordinates 26521..27265 in synthetic chromosome yeast_chr3_3_23)
discontinued: false
dominant: false
group u list: _385_
m user id: 0
owner id: 2669
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Constructed using overlap extension PCR protocol described in RFC38
star: false
creator: James DiCarlo
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K277037_sequence/1

BBa_K277038
type: ComponentDefinition
persistent identity: BBa K277038
display id: BBa_K277038
version: 1
was generated by: iGEM to SBOL conversion
description: 3L.3_23.B1.12
created: 2009-10-20T11:00:00Z
modified: 2015-05-08T01:11:46Z
mutable provenance: Synthetic Yeast
owned by: james
owned by: myers
mutable description: 3L.3_23.B1.12 is 739 bases long and is cloned into the pGem-T vector. 3L.3_23.B1.12 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.B1.12 is a constituent of 3L.3_23.B1 (along with 3L.3_23.B1.01, 3L.3_23.B1.02, 3L.3_23.B1.03, 3L.3_23.B1.04, 3L.3_23.B1.05, 3L.3_23.B1.06, 3L.3_23.B1.07, 3L.3_23.B1.08, 3L.3_23.B1.09, 3L.3_23.B1.10, 3L.3_23.B1.11, 3L.3_23.B1.13, and 3L.3_23.B1.14.) This part contains at least part of the following features (positions offset from first base of sequence): kind and name offset notes loxP_site loxPsym_YCL051W (569..602) forward_primer YCL051W_tagf1v1 (169..196) gene YCL051W (-1186..565) Protein involved in control of cell wall structure and stress response%3B inhibits Cbk1p protein kinase activity%3B overproduction confers resistance to cell-wall degrading enzymes reverse_primer YCL051W_tagr1v1 (424..451) Sequence (the first 739 bases correspond to coordinates 27255..27993 in synthetic chromosome yeast_chr3_3_23)
discontinued: false
dominant: false
group u list: _385_
m user id: 0
owner id: 2669
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Constructed using overlap extension PCR protocol described in RFC38
star: false
creator: James DiCarlo
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K277038_sequence/1

BBa_K277039
type: ComponentDefinition
persistent identity: BBa K277039
display id: BBa_K277039
version: 1
was generated by: iGEM to SBOL conversion
description: 3L.3_23.B1.13
created: 2009-10-20T11:00:00Z
modified: 2015-05-08T01:11:46Z
mutable provenance: Synthetic Yeast
owned by: james
owned by: myers
mutable description: 3L.3_23.B1.13 is 869 bases long and is cloned into the pGem-T vector. 3L.3_23.B1.13 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.B1.13 is a constituent of 3L.3_23.B1 (along with 3L.3_23.B1.01, 3L.3_23.B1.02, 3L.3_23.B1.03, 3L.3_23.B1.04, 3L.3_23.B1.05, 3L.3_23.B1.06, 3L.3_23.B1.07, 3L.3_23.B1.08, 3L.3_23.B1.09, 3L.3_23.B1.10, 3L.3_23.B1.11, 3L.3_23.B1.12, and 3L.3_23.B1.14.) This part contains at least part of the following features (positions offset from first base of sequence): kind and name offset notes gene YCL050C (125..+1090) Diadenosine 5'%2C5''-P1%2CP4-tetraphosphate phosphorylase I (AP4A phosphorylase)%2C involved in catabolism of bis(5'-nucleosidyl) tetraphosphates%3B has similarity to Apa2p reverse_primer YCL050C_tagr1v1 (620..647) mutation_affecting_coding_sequence YCL050C_re_remove_MmeI (680..691) removal of MmeI forward_primer YCL050C_tagf1v1 (293..320) loxP_site loxPsym_YCL050C (88..121) Sequence (the first 869 bases correspond to coordinates 27983..28851 in synthetic chromosome yeast_chr3_3_23)
discontinued: false
dominant: false
group u list: _385_
m user id: 0
owner id: 2669
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Constructed using overlap extension PCR protocol described in RFC38
star: false
creator: James DiCarlo
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K277039_sequence/1

BBa_K277040
type: ComponentDefinition
persistent identity: BBa K277040
display id: BBa_K277040
version: 1
was generated by: iGEM to SBOL conversion
description: 3L.3_23.B1.14
created: 2009-10-20T11:00:00Z
modified: 2015-05-08T01:11:46Z
mutable provenance: Synthetic Yeast
owned by: james
owned by: myers
mutable description: 3L.3_23.B1.14 is 804 bases long and is cloned into the pGem-T vector. 3L.3_23.B1.14 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.B1.14 is a constituent of 3L.3_23.B1 (along with 3L.3_23.B1.01, 3L.3_23.B1.02, 3L.3_23.B1.03, 3L.3_23.B1.04, 3L.3_23.B1.05, 3L.3_23.B1.06, 3L.3_23.B1.07, 3L.3_23.B1.08, 3L.3_23.B1.09, 3L.3_23.B1.10, 3L.3_23.B1.11, 3L.3_23.B1.12, and 3L.3_23.B1.13.) This part contains at least part of the following features (positions offset from first base of sequence): kind and name offset notes gene YCL050C (-735..230) Diadenosine 5'%2C5''-P1%2CP4-tetraphosphate phosphorylase I (AP4A phosphorylase)%2C involved in catabolism of bis(5'-nucleosidyl) tetraphosphates%3B has similarity to Apa2p Sequence (the first 804 bases correspond to coordinates 28843..29646 in synthetic chromosome yeast_chr3_3_23)
discontinued: false
dominant: false
group u list: _385_
m user id: 0
owner id: 2669
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Constructed using overlap extension PCR protocol described in RFC38
star: false
creator: James DiCarlo
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K277040_sequence/1

https://synbiohub.org/public/igem/BBa_K277136_sequence/1
type: Sequence
persistent identity: BBa K277136 sequence
display id: BBa_K277136_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcttagctcaattaaatatactagtaaataagtatataaacaataattttgcattttattttactggaaactcttctcaaaaccaaattgcgcaaggattgattcagtacaattatgcaaactcgaaaagtaaataaacaaaaagatacagttgagtgaaaggtaaataaacattattctacttgattttcctctgttttttccatttttcttttttgtcttttcgtgttctagcgtgttcactttttcagccatgtaattacttaaataggaagtttacacgatgttctcgcataaaaagctggaagtgcagaacaaagaggaagaaaataggaacagagaatttgtttgtttatttgtttgtttgcttgtttgtttgtttgttttgatttgaagtcgaggtacttattcgaaataattattttgcccagggttttggatagttttttgttttccaattagatttttagaaatattttatcatagttttttgtttatttatttgtttatttatttgttgatttgtttatttatttgttggtttgtttatttaagtttgtaaatgtttaataaacaaaacaatatttgcaagccattgaaaactttgtaaatttgtttgagaatttacgaaacaatgaaaattaaggactctttgatggtgaacgaattactactttgcagcaggtaattctctaaaactcagaaacaaggcatgtatttttcactaggaaaaagaaggaaaataaggaaaatgggactacagtattagggaaacaggattaaaattcgtaggtaataataacccatactttcattgtagcagtactttcgttaataaaatggatattgttgtacttcggtagatgttatattctaaagagttaaattatccaattccaaattctagggacggtttccaaagatttgtattgtccagtgacataattttcactaaaacattgatctgtttaataggtttgctataaatgcacaataatttttcacccatgatacccaatttcaagagaagcaattgctacatataattatttaggctttactatctactactcattgactgtgcccttttacacaattataacaaatatgtcaaagcagatgccatgaactttgtatctgaatttttgatttccttttaattctaattgcagacgacgtaaatatagttctgaatttcaaagtcactgttaattaattgttctaattgtttggtttttttaatataaatcactagtgcttaagttctgttgacgcacacagtacctatctttgattccttcgtgcaaacagtattccggcacgttaattgataccaaaaagatttcaaaatcagatgcttcaagactaattgtatactttatgtgacttctggagtttaatttgttgtattgatgagataatattgtctattatattgccaataacaaccagcgccaggtgtactaagtcgagatgacagaaaatttataataagtgtaaatacaataaatacaataaattttgtaaataaataactacttcaatagaaatctcaaagtcgaagaaacacagaacacagaatgaaactggactaaatgattttcaataaaggaaaagcacgaaaaattttttgaacttttttgggacagcgtggatcgtgtaattactgtattgggaaatttacacgattctcgacaaataaagaaaaaggcgcgtttaaagacttacaagcacacctttgaattatttttgttctctatgattatcaaatgtgctagtgtttgcaagatggtgcttttttttctttagattgccacgtttctgctcctctttgtggttttctttttttttttcaatcttacttataaaatgaatatttcaattgatgaatagctatatattggatacaaaaattagcatttaatcgaaaactgcagcatgtatttaatcgaaaactacagcatgtagctatgatacgacagaagatttttgtttttata…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K277027_sequence/1
type: Sequence
persistent identity: BBa K277027 sequence
display id: BBa_K277027_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcttagctcaattaaatatactagtaaataagtatataaacaataattttgcattttattttactggaaactcttctcaaaaccaaattgcgcaaggattgattcagtacaattatgcaaactcgaaaagtaaataaacaaaaagatacagttgagtgaaaggtaaataaacattattctacttgattttcctctgttttttccatttttcttttttgtcttttcgtgttctagcgtgttcactttttcagccatgtaattacttaaataggaagtttacacgatgttctcgcataaaaagctggaagtgcagaacaaagaggaagaaaataggaacagagaatttgtttgtttatttgtttgtttgcttgtttgtttgtttgttttgatttgaagtcgaggtacttattcgaaataattattttgcccagggttttggatagttttttgttttccaattagatttttagaaatattttatcatagttttttgtttatttatttgtttatttatttgttgatttgtttatttatttgttggtttgtttatttaagtttgtaaatgtttaataaacaaaacaatatttgcaagccattgaaaactttgtaaatttgtttgagaatttacgaaacaatgaaaattaaggactctttgatggtgaacgaattactactttgcagcaggtaattctctaaaactcagaaacaaggcatgtatttttcactaggaaaaagaaggaaaat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K277028_sequence/1
type: Sequence
persistent identity: BBa K277028 sequence
display id: BBa_K277028_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaggaaaatgggactacagtattagggaaacaggattaaaattcgtaggtaataataacccatactttcattgtagcagtactttcgttaataaaatggatattgttgtacttcggtagatgttatattctaaagagttaaattatccaattccaaattctagggacggtttccaaagatttgtattgtccagtgacataattttcactaaaacattgatctgtttaataggtttgctataaatgcacaataatttttcacccatgatacccaatttcaagagaagcaattgctacatataattatttaggctttactatctactactcattgactgtgcccttttacacaattataacaaatatgtcaaagcagatgccatgaactttgtatctgaatttttgatttccttttaattctaattgcagacgacgtaaatatagttctgaatttcaaagtcactgttaattaattgttctaattgtttggtttttttaatataaatcactagtgcttaagttctgttgacgcacacagtacctatctttgattccttcgtgcaaacagtattccggcacgttaattgataccaaaaagatttcaaaatcagatgcttcaagactaattgtatactttatgtgacttctggagtttaatttgttgtattgatgagataatattgtctattatattgccaataacaaccagcgccaggtgtactaagtcgagatgacagaaaatttataataagtgtaaatacaat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K277029_sequence/1
type: Sequence
persistent identity: BBa K277029 sequence
display id: BBa_K277029_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaatacaataaattttgtaaataaataactacttcaatagaaatctcaaagtcgaagaaacacagaacacagaatgaaactggactaaatgattttcaataaaggaaaagcacgaaaaattttttgaacttttttgggacagcgtggatcgtgtaattactgtattgggaaatttacacgattctcgacaaataaagaaaaaggcgcgtttaaagacttacaagcacacctttgaattatttttgttctctatgattatcaaatgtgctagtgtttgcaagatggtgcttttttttctttagattgccacgtttctgctcctctttgtggttttctttttttttttcaatcttacttataaaatgaatatttcaattgatgaatagctatatattggatacaaaaattagcatttaatcgaaaactgcagcatgtatttaatcgaaaactacagcatgtagctatgatacgacagaagatttttgtttttatagttaagtcaagaagaaattctatttgtccagcaatccggcgcaaagaagactactaaagggtattgaaaaatttctagaaccagaaataatcgaaagcattactttttttggctctataaaggagttctggacagcttgaacttccgaagtctgtctgtttagctcgttaagataaatctcaaaagagtagcaagcaggtgggagggataatttggattaaagtgtatatattgcgtatatatagccacgtaaacctcacact
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K277030_sequence/1
type: Sequence
persistent identity: BBa K277030 sequence
display id: BBa_K277030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aacctcacactattgagtttaaacatttttcaccttacgttattattcttttgtctaattttaatctcctccagtatactaaaaccgcaaaagcgggttacccagcttgtttctttttgtctgaaaaattttttgcgatgcccattatgaaaaattggcaataatagtatagtagttagtttaagtttagattctttcaatactcattctgcttcagtttgtagttagatttaactcaatagaggtgattggcaaaaatgggtaagacacaaaagaagaatagtaagggacgtttagatagatactattatctagcaaaggagaaaggttatcgtgctcgttcatcctttaagattattcagattaatgaaaagtatggccacttcttagaaaaatcgaaggttgttattgatctgtgtgctgctcctggttcatggtgtcaagtcgctagcaagttgtgcccagttaatagcttaattattggtgttgatattgttccaatgaagccgatgcccaatgttataacttttcaaagtgatattacaacagaagattgtagatcaaaattgaggggttatatgaagacttggaaagctgatacagtgttgcatgatggtgctcctaatgttggtttgggttgggttcaggatgctttcacccaatctcaattaaccttacaagctttgaagttggccgtcgaaaatttggtggtgaacggtacttttgttactaaaattttcagatccaaggatt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K277031_sequence/1
type: Sequence
persistent identity: BBa K277031 sequence
display id: BBa_K277031_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atccaaggattataataaattgatttgggtttttcaacaattgtttgagaaagttgaagccacaaaaccacccgcatcaagaaatgtttctgcagaaatttttgtagtgtgtaaaggtttcaaagctcctaaacgattggatcctcgattgctggatccaaaagaagtttttgaagaattgccagatgggcaacagaatatggagtccaagatttataatcccgaaaaaaaagttagaaaaagacaaggttatgaggaaggtgataatttactgtatcatgaaacctcaattttggattttgtcaggacggaagacccaataagcatgcttggagaaatgaataagtttacaattgatgagaatgaccatgaatggaagatcttgaagaaattgaaacaaaccacagacgagttccgttcctgtattgaagatttaaaagtcttgggtaaaaaggattttaaaatgatcctaagatggagaaagattgcaagggaaatcttgggcatcgaagttaaggacgacgctaaaacagaaattgaagtagtaccgttaacagaagaggagcaaattgaaaaagatttgcaaggtttacaggagaagcagcgtctaaatgtcaagcgcgaaagaagaaggaagaacgaaatgaagcaaaaggaactacagagaatgcaaatgaacatgataacccccactgatattggtatt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K277032_sequence/1
type: Sequence
persistent identity: BBa K277032 sequence
display id: BBa_K277032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attggtattgaagccgcaagtttgggtaaagaatcgttgtttaatttgaaaactgcagaaaagactggtatcttgaacgacttggcgaagggtaagaaaagaatgatttttacagacgatgaattagccaaagataatgatatttacattgatgaaaacatcatgatcaaagataaggattctgccgctgatgcggacgatttagaaagcgagttgaatgccatgtatagtgattataaaacccgacgaagcgaacgagacgctaaattcagagctaaacaagcacgtggtggcgataatgaggaagaatggaccggttttaatgagggaagtctagaaaagaaagaagaggaaggtaaagattatatcgaagacaatgacgatgaaggtgtcgaaggagactccgatgatgatgaagccatcaccaatttgattagcaaattgaagggacaagaaggtgatcacaaattgagtagtaaggcacgtatgattttcaatgatccgatatttaataacgttgaacctgatttgccagtaaataccgtcaatgatggcataatgagttccgagtctgttggtgatatttctaaattaaataagaaaagaaaacatgaagaaatgcaccagaaacaagatgaagcagattccagcgacgaatctagctctgacgatagcgatttcgaaattgtggccaacgataatgcatcagaagaattcgattctgattatgattcagaggaagaaaaaaat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K277033_sequence/1
type: Sequence
persistent identity: BBa K277033 sequence
display id: BBa_K277033_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aagaaaaaaatcaaacaaagaaagaaaagcattccagagacattgatattgctactgttgaagccatgactttagctcaccaattggctctaggccaaaagaacaagcatgatcttgttgatgaaggtttcaatagatacaccttccgtgacaccgaaaatttgccagattggtttttagaagatgaaaaggaacattcaaagataaataagccgattactaaggaggcagcgatggcaattaaagaaaaaataaaggcaatgaacgcccgtcctattaagaaggttgctgaagctaaagcaagaaagaggatgcgtgccgtagctcggttagaaaagatcaagaagaaagctggtctaatcaatgacgacagcgataagacagagaaggacaaggctgaagaaatttctagattgatgcgtaaggttaccaaaaaaccaaagaccaagccaaaggttactttggttgttgcctcaggtaggaacaaaggtttggcaggtagaccaaagggcgttaagggtaagtataagatggttgatggtgtcatgaagaatgaacaaagagccctaagacgtatcgcaaagaagcatcacaagaaaaagtaataacaaagagcattatttttccattcccttgtataatattgaaaataaaggaaaagaaagcaatagatatattatgtatacatagaattaatcaaattatcatcaaaaaacttataggaaacctatcgttttgaattcagaaaat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K277034_sequence/1
type: Sequence
persistent identity: BBa K277034 sequence
display id: BBa_K277034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attcagaaaatacgagtgcacagtagaacaatttttattcccgttttactgatcttttcttcttgttgttaccaaataccttggagaaaatgtatatgatggagatgagtaagcatagtaacgtaccatttttgatcttggaataatcctttgtgtcaggccttggaattggtactagaagagttgtcgagttcaaatggcggaatgtagtatcgtctgtaaagagagattcatatcctagaccttttttataaaatggattacgggaaactttattgtcacctgtgtcgcaggctaatataacttctggatcaaatgaaacttctaatttatcccctttattattagaaggctcaatatatctagtatgcaatgtcacttcattcattgtgccggctttcaattcaaagatactttcagaaccccatgccttatctcgaagagagtattctggtaattctaagtcgtcttctccaaaaagtagtaagggagaggattggaatttatcgataaataattctaacggcaattgcaggtgcattagatacatgcatttagggcgttcttcgaaatctgtgagatcaatcatgatttttggatgcaaaccgataggttcattcaagtacaagctggtggtggtggtgctatgattgtatgcgatatggccctgtttatacaataaggatgttttctgacatctttccattttaccatcgtccatcctccaattacaacgtattccaccaatatcgacatcactttcatcacttgcatccacaaagaatacacctacttcgagtttct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K277035_sequence/1
type: Sequence
persistent identity: BBa K277035 sequence
display id: BBa_K277035_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agtttctcatctttcactcttgcaattgtaaattcttcaccctgtttcaacagacgatattcaacaatttggatttgtgtgggctccactgatatatcatatgtaaaatcttctggattccatgacagattcaaatctacttcgggaggcaagtactgctctatgtcaaacttctcactgtgaaatgaattgtagactgggttagtaataaatcgttctggaacgtcgctgctgttgctataaactgaaaagccagcactcagttcattttctataacgcttaaacttctagccaagtccgctctgggcctccaagtgattctcgtaaaggatttatccaagcttccagtcctctctaatagccacctttgttgtaaaaccacaccagaacctccacgaacagtcaaatgagtgtcattttggcgcatatgattaccgatatcctcaggggcattgtagagtacagtcactctatgtcttgtcaccattactcagctagaaacgcacacttcttgcgtttattctcctcccgatttgattatgattagcgtttccattatttgatcttttttatcagggggatcttcaaagaaaggaactttttttaggaaaaaaaaaataatcatcttttattacatgtgtaatacttcaagcaatcataaacaagagttaagaaggcaaagttctgctgttagtgtgtcctccaatactcttgcacgacaaagttttagttatccccttctacgtgtattgt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K277036_sequence/1
type: Sequence
persistent identity: BBa K277036 sequence
display id: BBa_K277036_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acgtgtattgttcctccgtggtcgattgtgttcaaagattgggagctcttttcgtgttttgcactaagagcatactgttaatccgcaaaggccactaaaacttacctgcatttgtctacttaaagatacattccaactatttacattcatttttatttctaaaagagcattctgaaccctcggcaattatttcatactccttctcagaataggaaaatgcccaatacgcatactcaacatgtgcaaatatcagagccaaatcctgtaaatactttgtctacaccatccaaaagaggtcaccgccatcgcagatcgctagcaatatcaggagattttgattttttgaaacagcctgcagcaattgtgaatttaccacctccacaggcggctgaaaattgtccttcaactgccccaactgctgtatcaagtacattatcgccaatacgctacaatagatttccttgcaaaaccaatgaagacgctggaacgttagatttgcctgaaccaagattttatccgttatcaccaaagaacaatctgcaaacaccaagtccacgatttttcattagtgaagagccaagtttttcatcgccagttaaaggcgtcccagatgccattattaaccttgatgacgctctaaaaacccgaccacgaagctttaaatcacatagaagatct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K277037_sequence/1
type: Sequence
persistent identity: BBa K277037 sequence
display id: BBa_K277037_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agaagatctgaatccgctcctcctgatttggaggttatggtagataagggcaattgtgcagccggttctaactctatgattaaagaagaagaggactccttaattgaaccagaatcgaaaaatgaatattatgagcaaaagctgccaacagcactattatccccactgcggccttccctttgtgtatctgaacaggccattgatgtagatgattcagctctcaatgggtcaccgacccatcacaaccatgggatgcaaaacgccaatgcacggaattctaacacattcaattcgttgaagatcaaaggccaaaagcaaagatattatcattatacgaagcagctacctttgaccgtaggctgtgactcgcaatctccaaaagaacaaaggtcggctgcttcaatgacaatcaatcaggcaatgacaccaagcagcttggcttacactccaagcaagctagcatctactcccgcaacaccagtatccttttatgacagcaatgcggacattaacttagaaagtgataattttccactaaaagataaccctagatatgccaaggatggttatcctaaaaagtgcggcaattcacagcttaatcgtgtgctggatagcgataaaagacaggattttagtggagaatcgagaagaagaagatcgggcagtcctatctcccacatgcaacaccgcaacctgattgataatatgaaaggtagacgaaacagtaacacgataaactcaatct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K277038_sequence/1
type: Sequence
persistent identity: BBa K277038 sequence
display id: BBa_K277038_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaactcaatcttcaactacaagagtcaacattatgaaatgccatatgatgatatgatgaaaaatgaaaacattaatgcacagtccatgcccttttcagtcaacggtgtcaacaatgaaaatagtatcggaggggttattacgagagcggacgatgcaccccttcaacatagcgttgttaagagctgcaccccagacggcaaggaagaaatgaataggcttaaaagtaatgacagtaatgaatattccaagtctgaagggcagatcagaaccaattcgcaactaagtaaggacattctcatgggtgaaccaggtgatatggttgatctgtcctcttttgtcaacgcgcagagaaaagcctcaaatgaaactggtgacttagtctttagtttatcccaggatgatgacgcactgaaaacgttccacgcttctaattctgctgctacctctaacgaaagctggtgtattagcgatgatgcgttaggaaagcaggcgcaggacagtgaagttaggaggaaaagaaaatccaaattaggactctttagacatattttttcaaggaagtaatacataacttcgtataatgtacattatacgaagttataacaatattaataataagtaataaaaaggtgaatcgtaaccttaacggcattgattgcatgtagttttaataataaagtgtagaataataaatataaaatttcaaagtcatcagaaataaaaattgaatagacatat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K277039_sequence/1
type: Sequence
persistent identity: BBa K277039 sequence
display id: BBa_K277039_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aatagacatatacatatagaaattacttctatcatttaaatacatatatatacatacatgtatacgtgtatacttgtacttgtgaagataacttcgtataatgtacattatacgaagttatgactcaatagttgtattcgtttggtttttgaccagaagtgtttgggaaaccacattccaataagatatcgttaatcaattcaggtttttcagtaatcttggagaaaacttcatcatttttggttaagatcataccacaataacctgtggagttgaaacctatcttcatttcgtcgctgaaagctttgcttcgagggacaacacagatccattccttggttaacataagattgtaggatttctttagttctggattttcgttcaaccagtcctggaaaaaggtcaaagctctttgcaatatggagatgtaacacatagctaataggtcttcatcaacagtttcttcagactccggcattggcaagacaaaatgagcgaacgagactttagcatcttgcaaaggttcagtattgaaagttggtaggaaatgttctttaccattacatagtctatcttggaaagtgacgaacttttcaggcatttgcagaatttgcaaatgcttatgatctaggctgctgccagaagcaggaccagaattgtaaaagaccatgtgtctcttatcggattcttcattgtccaaggcacacagcaacttataagcagtcaataaatcggttggggtcaaggcatcagtttgatgttggtattcgttagttaccaataaagtgtgttcagggattacagggaatttgtttaatagcaatttatacgctttgttatcggcaccaccaaattctgggataaccgtt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K277040_sequence/1
type: Sequence
persistent identity: BBa K277040 sequence
display id: BBa_K277040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ataaccgttaattcttcctcaggtttgcctagtggatcctctccttctggagtttggccacgctctggcttttcgatcagacttggcatgtgactaatcaagtatggcatgctggtttttgggtcctttgttttcgttgtttcagtctggataaattttaagttaccattatcgaaggcacttttgtacttgtcactaattaaagatgcaatgtcagcggggatactcatttttattttaatttttacttttctgtttgttctaaaatctatctaaactggctttcaagatcaatctattgtcttttaaggtaaactttaaattggaaataatagtaatgttagttccttcattttaaccttgtattgtatttcctttgcgtgatgaaaaaaaaactgaaaaagagaaaaataagaaaatcttctagaacgttccgaaacaggacacttagcacacaaatacagaataggaaagtaaaaggcaatatatgaatgcagtgcttgtaactggtgcttgtatccaagaatagcttcttgctgtaggttatgggaatatcgtgtaagctggggtgacttttgagctattcgcgacgcccgacgccgtaataactactttcgacagaccacttatgacagtatttcaggccgctcttataaaatgacatgttaacaaacagttctgattattcgccttttgacaggacgataatgtaaatagttgtggtagtatcattcaggtatgtaactgtttactttgtatcgcttgaaaaaaataagcatttcagagccttctttggagctcaag
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z