LOCUS       BBa_K283001             2996 bp    DNA     linear       12-May-2024
DEFINITION  pT7-rbs-(sp_eGFP_streptavidin_AIDAc)-Terminators
ACCESSION   BBa_K283001
VERSION     BBa_K283001.1  
FEATURES             Location/Qualifiers
     promoter        1..46
                     /label=BBa_I712074
     misc_feature    59..62
                     /label=conserved
     RBS             55..66
                     /label=BBa_B0034
     stem_loop       2879..2922
                     /label=stem_loop
     misc_feature    2868..2947
                     /label=BBa_B0010
     terminator      2868..2947
                     /label=BBa_B0010
     stem_loop       2963..2982
                     /label=T7 TE
     misc_feature    2989..2989
                     /label=stop
     polyA_site      2983..2996
                     /label=polya
     misc_feature    2956..2996
                     /label=BBa_B0012
     terminator      2956..2996
                     /label=BBa_B0012
ORIGIN
        1 taatacgact cactataggg aatacaagct acttgttctt tttgcatact agagaaagag
       61 gagaaatact agatgacagg atcatttagg atccaggaaa caatgaataa ggcctacagt
      121 atcatatgga gccactccag acaggcctgg attgtggcct cagagttagc cagaggacat
      181 ggttttgtcc ttgcaaaaaa tacactgctg gtattggcgg ttgtttccac aatcggaaat
      241 gcatttgcaa tggaattcat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
      301 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
      361 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
      421 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc
      481 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
      541 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
      601 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
      661 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
      721 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac
      781 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
      841 ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag
      901 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
      961 gacgagctgt acaaggagct cgctgaagct ggtatcaccg gcacctggta caaccagctg
     1021 ggatccacct tcatcgttac cgctggtgct gacggtgctc tgaccggtac ctacgaatcc
     1081 gctgttggta acgctgaaag ccgctacgtt ctgaccggtc gttacgactc cgctccggct
     1141 accgacggtt ccggaaccgc tctgggttgg accgttgctt ggaaaaacaa ctaccgtaac
     1201 gctcactccg ctaccacctg gtctggccag tacgttggtg gtgctgaagc tcgtatcaac
     1261 acccagtggt tgttgacctc cggcaccacc gaagccaacg cgtggaaatc caccctggtt
     1321 ggtcacgaca ccttcaccaa agttaaaccg tccgctgctt ccgagctcaa taacaatgga
     1381 agcattgtca ttaataacag cattataaac gggaatatta cgaatgatgc tgacttaagc
     1441 tttggtacag caaagctgct ctctgctaca gtgaatggta gtcttgttaa taacaaaaat
     1501 atcattctta atcctacaaa agaaagtgca ggtaatactc ttaccgtgtc aaattatact
     1561 gggacaccgg gaagtgttat ttctcttggt ggtgtgcttg aaggagataa ttcacttacg
     1621 gaccgtctgg tggtgaaagg taatacctct ggtcaaagtg acatcgttta tgtcaatgaa
     1681 gatggcagtg gtggtcagac gagagatggt attaatatta tttctgtaga gggaaattct
     1741 gatgcagaat tctctctgaa gaaccgcgta gttgccggag cttatgatta cacactgcag
     1801 aaaggaaacg agagtgggac agataataag ggatggtatt taaccagtca tcttcccaca
     1861 tctgataccc ggcaatacag accggagaac ggaagttatg ctaccaatat ggcactggct
     1921 aactcactgt tcctcatgga tttgaatgag cgtaagcaat tcagggccat gagtgataat
     1981 acacagcctg agtctgcatc cgtgtggatg aagatcactg gaggaataag ctctggtaag
     2041 cttaatgacg ggcaaaataa aacaacaacc aatcagttta tcaatcagct cgggggggat
     2101 atttataaat tccatgctga acaactgggt gattttacct tagggattat gggaggatac
     2161 gcgaatgcaa aaggtaaaac gataaattac acgagcaaca aagctgccag aaacacactg
     2221 gatggttatt ctgtcggggt atacggtacg tggtatcaga atggggaaaa tgcaacaggg
     2281 ctctttgctg aaacttggat gcaatataac tggtttaatg catcagtgaa aggtgacgga
     2341 ctggaagaag aaaaatataa tctgaatggt ttaaccgctt ctgcaggtgg gggatataac
     2401 ctgaatgtgc acacatggac atcacctgaa ggaataacag gtgaattctg gttacagcct
     2461 catttgcagg ctgtctggat gggggttaca ccggatacac atcaggagga taacggaacg
     2521 gtggtgcagg gagcagggaa aaataatatt cagacaaaag caggtattcg tgcatcctgg
     2581 aaggtgaaaa gcaccctgga taaggatacc gggcggaggt tccgtccgta tatagaggca
     2641 aactggatcc ataacactca tgaatttggt gttaaaatga gtgatgacag ccagttgttg
     2701 tcaggtagcc gaaatcaggg agagataaag acaggtattg aaggggtgat tactcaaaac
     2761 ttgtcagtga atggcggagt cgcatatcag gcaggaggtc acgggagcaa tgccatctcc
     2821 ggagcactgg ggataaaata cagcttctga ctcgagtaat actagagcca ggcatcaaat
     2881 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
     2941 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata
//