LOCUS       BBa_K283002             1986 bp    DNA     linear       22-Dec-2024
DEFINITION  Speed control device (pLacI-rbs-TetR-terminator-ptet-rbs-chez_his-d
            ouble terminators)
ACCESSION   BBa_K283002
VERSION     BBa_K283002.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             230..846
                     /label=tetR
     misc_feature    847..880
                     /label=SsrA
     misc_feature    887..911
                     /label=Help:Barcodes
     CDS             227..911
                     /label=BBa_C0040
     stem_loop       931..974
                     /label=stem_loop
     misc_feature    920..999
                     /label=BBa_B0010
     terminator      920..999
                     /label=BBa_B0010
     stem_loop       1015..1034
                     /label=T7 TE
     misc_feature    1041..1041
                     /label=stop
     polyA_site      1035..1048
                     /label=polya
     misc_feature    1008..1048
                     /label=BBa_B0012
     terminator      1008..1048
                     /label=BBa_B0012
     misc_feature    1057..1075
                     /label=TetR 1
     misc_feature    1057..1110
                     /label=BBa_R0040
     promoter        1076..1081
                     /label=-35
     misc_feature    1082..1100
                     /label=TetR 2
     promoter        1099..1104
                     /label=-10
     promoter        1057..1110
                     /label=BBa_R0040
     misc_feature    1123..1126
                     /label=conserved
     RBS             1119..1130
                     /label=BBa_B0034
     CDS             1137..1781
                     /label=cheZ
     misc_feature    1137..1831
                     /label=BBa_K094100
     misc_feature    1832..1849
                     /label=6-His tag
     CDS             1137..1849
                     /label=BBa_K283008
     stem_loop       1869..1912
                     /label=stem_loop
     misc_feature    1858..1937
                     /label=BBa_B0010
     terminator      1858..1937
                     /label=BBa_B0010
     stem_loop       1953..1972
                     /label=T7 TE
     misc_feature    1979..1979
                     /label=stop
     polyA_site      1973..1986
                     /label=polya
     misc_feature    1946..1986
                     /label=BBa_B0012
     terminator      1946..1986
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgt ccagattaga
      241 taaaagtaaa gtgattaaca gcgcattaga gctgcttaat gaggtcggaa tcgaaggttt
      301 aacaacccgt aaactcgccc agaagctagg tgtagagcag cctacattgt attggcatgt
      361 aaaaaataag cgggctttgc tcgacgcctt agccattgag atgttagata ggcaccatac
      421 tcacttttgc cctttagaag gggaaagctg gcaagatttt ttacgtaata acgctaaaag
      481 ttttagatgt gctttactaa gtcatcgcga tggagcaaaa gtacatttag gtacacggcc
      541 tacagaaaaa cagtatgaaa ctctcgaaaa tcaattagcc tttttatgcc aacaaggttt
      601 ttcactagag aatgcattat atgcactcag cgctgtgggg cattttactt taggttgcgt
      661 attggaagat caagagcatc aagtcgctaa agaagaaagg gaaacaccta ctactgatag
      721 tatgccgcca ttattacgac aagctatcga attatttgat caccaaggtg cagagccagc
      781 cttcttattc ggccttgaat tgatcatatg cggattagaa aaacaactta aatgtgaaag
      841 tgggtccgct gcaaacgacg aaaactacgc tttagtagct taataacact gatagtgcta
      901 gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg
      961 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca
     1021 ccttcgggtg ggcctttctg cgtttatata ctagagtccc tatcagtgat agagattgac
     1081 atccctatca gtgatagaga tactgagcac tactagagaa agaggagaaa tactagatga
     1141 tgcaaccatc aatcaaacct gctgacgagc attcagctgg cgatatcatt gcgcgcatcg
     1201 gcagcctgac gcgtatgctg cgcgacagtt tgcgggaact ggggctggat caggccattg
     1261 ccgaagcggc ggaagccatc cccgatgcgc gcgatcgttt gtactatgtt gtgcagatga
     1321 ccgcccaggc tgcggagcgg gcgctgaaca gtgttgaggc gtcacaaccg catcaggatc
     1381 aaatggagaa atcagcaaaa gcgttaaccc aacgttggga tgactggttt gccgatccga
     1441 ttgaccttgc cgacgcccgt gaactggtaa cagatacacg acaatttctg gcagatgtac
     1501 ccgcgcatac cagctttact aacgcgcaac tgctggaaat catgatggcg caggattttc
     1561 aggatctcac cgggcaggtc attaagcgga tgatggatgt cattcaggag atcgaacgcc
     1621 agttgctgat ggtgctgttg gaaaacatcc cggaacagga gtcgcgtcca aaacgtgaaa
     1681 accagagttt gcttaatgga cctcaggtcg ataccagcaa agccggtgtg gtagccagtc
     1741 aggatcaggt ggacgatttg ttggatagtc ttggattttg agctagcaac gcaatgacgt
     1801 cggaattgcc agctggggcg ccctctggta acatcatcac catcaccact actagagcca
     1861 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
     1921 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg
     1981 tttata
//