LOCUS       BBa_K283014             2400 bp    DNA     linear       26-Nov-2024
DEFINITION  pLacIq-rbs-lacI-T-pLacI-rbs-cheZ_histag-double terminators
ACCESSION   BBa_K283014
VERSION     BBa_K283014.1  
FEATURES             Location/Qualifiers
     promoter        1..80
                     /label=pLacIq
     promoter        1..80
                     /label=BBa_K094140
     misc_feature    93..96
                     /label=conserved
     RBS             89..100
                     /label=BBa_B0034
     CDS             107..1154
                     /label=protein
     CDS             107..1154
                     /label=BBa_K094142
     terminator      1163..1316
                     /label=BBa_K094014
     CDS             1325..1412
                     /label=end of LacI coding region (inactive)
     misc_feature    1325..1524
                     /label=BBa_R0010
     misc_feature    1413..1450
                     /label=CAP binding site
     promoter        1461..1466
                     /label=-35
     promoter        1485..1490
                     /label=-10
     misc_feature    1490..1524
                     /label=LacI binding site
     misc_feature    1497..1497
                     /label=start
     promoter        1325..1524
                     /label=BBa_R0010
     misc_feature    1537..1540
                     /label=conserved
     RBS             1533..1544
                     /label=BBa_B0034
     CDS             1551..2195
                     /label=cheZ
     misc_feature    1551..2245
                     /label=BBa_K094100
     misc_feature    2246..2263
                     /label=6-His tag
     CDS             1551..2263
                     /label=BBa_K283008
     stem_loop       2283..2326
                     /label=stem_loop
     misc_feature    2272..2351
                     /label=BBa_B0010
     terminator      2272..2351
                     /label=BBa_B0010
     stem_loop       2367..2386
                     /label=T7 TE
     misc_feature    2393..2393
                     /label=stop
     polyA_site      2387..2400
                     /label=polya
     misc_feature    2360..2400
                     /label=BBa_B0012
     terminator      2360..2400
                     /label=BBa_B0012
ORIGIN
        1 ttgacaccat cgaatggtgc aaaacctttc gcggtatggc atgatagcgc ccggaagaga
       61 gtcaattcag ggtggtgaat tactagagaa agaggagaaa tactagatgg tgaatgtgaa
      121 accagtaacg ttatacgatg tcgcagagta tgccggtgtc tcttatcaga ccgtttcccg
      181 cgtggtgaac caggccagcc acgtttctgc gaaaacgcgg gaaaaagtgg aagcggcgat
      241 ggcggagctg aattacattc ccaaccgcgt ggcacaacaa ctggcgggca aacagtcgtt
      301 gctgattggc gttgccacct ccagtctggc cctgcacgcg ccgtcgcaaa ttgtcgcggc
      361 gattaaatct cgcgccgatc aactgggtgc cagcgtggtg gtgtcgatgg tagaacgaag
      421 cggcgtcgaa gcctgtaaag cggcggtgca caatcttctc gcgcaacgcg tcagtgggct
      481 gatcattaac tatccgctgg atgaccagga tgccattgct gtggaagctg cctgcactaa
      541 tgttccggcg ttatttcttg atgtctctga ccagacaccc atcaacagta ttattttctc
      601 ccatgaagac ggtacgcgac tgggcgtgga gcatctggtc gcattgggtc accagcaaat
      661 cgcgctgtta gcgggcccat taagttctgt ctcggcgcgt ctgcgtctgg ctggctggca
      721 taaatatctc actcgcaatc aaattcagcc gatagcggaa cgggaaggcg actggagtgc
      781 catgtccggt tttcaacaaa ccatgcaaat gctgaatgag ggcatcgttc ccactgcgat
      841 gctggttgcc aacgatcaga tggcgctggg cgcaatgcgc gccattaccg agtccgggct
      901 gcgcgttggt gcggatatct cggtagtggg atacgacgat accgaagaca gctcatgtta
      961 tatcccgccg ttaaccacca tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga
     1021 ccgcttgctg caactctctc agggccaggc ggtgaagggc aatcagctgt tgcccgtctc
     1081 actggtgaaa agaaaaacca ccctggcgcc caatacgcaa accgcctctc cccgcgcgtt
     1141 ggccgattca ttaatactag agtgcagctg gcacgacagg tttcccgact ggaaagcggg
     1201 caggctgcaa acgacgaaaa ctacgcttta gtagcttaat aactctgata gtgctagtgt
     1261 agatctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact
     1321 agagcaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     1381 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
     1441 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
     1501 gtgagcggat aacaatttca cacatactag agaaagagga gaaatactag atgatgcaac
     1561 catcaatcaa acctgctgac gagcattcag ctggcgatat cattgcgcgc atcggcagcc
     1621 tgacgcgtat gctgcgcgac agtttgcggg aactggggct ggatcaggcc attgccgaag
     1681 cggcggaagc catccccgat gcgcgcgatc gtttgtacta tgttgtgcag atgaccgccc
     1741 aggctgcgga gcgggcgctg aacagtgttg aggcgtcaca accgcatcag gatcaaatgg
     1801 agaaatcagc aaaagcgtta acccaacgtt gggatgactg gtttgccgat ccgattgacc
     1861 ttgccgacgc ccgtgaactg gtaacagata cacgacaatt tctggcagat gtacccgcgc
     1921 ataccagctt tactaacgcg caactgctgg aaatcatgat ggcgcaggat tttcaggatc
     1981 tcaccgggca ggtcattaag cggatgatgg atgtcattca ggagatcgaa cgccagttgc
     2041 tgatggtgct gttggaaaac atcccggaac aggagtcgcg tccaaaacgt gaaaaccaga
     2101 gtttgcttaa tggacctcag gtcgatacca gcaaagccgg tgtggtagcc agtcaggatc
     2161 aggtggacga tttgttggat agtcttggat tttgagctag caacgcaatg acgtcggaat
     2221 tgccagctgg ggcgccctct ggtaacatca tcaccatcac cactactaga gccaggcatc
     2281 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
     2341 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
//