BBa_K283014
type: ComponentDefinition
persistent identity: BBa K283014
display id: BBa_K283014
version: 1
was generated by: iGEM to SBOL conversion
description: pLacIq-rbs-lacI-T-pLacI-rbs-cheZ_histag-double terminators
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:47Z
mutable provenance: The prosseA promoter is self-designed The rbs is from the registry
owned by: james
owned by: myers
mutable description: prosseA promoter with a rbs. This part is similar to K283012, but with a different plasmid backbone pSB3C5
discontinued: false
dominant: false
experience: experience/Works
group u list: _383_
m user id: 0
owner id: 5494
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: BBa_K283014
star: false
creator: W Ling , Nelson Chu, Shi Lei
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_B0012
component: BBa_K094014
component: BBa_K094142
component: BBa_B0034
component: BBa_R0010
component: BBa_K094140
component: BBa_B0010
component: BBa_K283008
sequence annotation: BBa_K094140
sequence annotation: BBa_B0034
sequence annotation: BBa_K094142
sequence annotation: BBa_K094014
sequence annotation: BBa_R0010
sequence annotation: BBa_K283008
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K283014_sequence/1

BBa_B0012
type: Component
persistent identity: component2056035
display id: component2056035
version: 1
top level: BBa_K283014
definition: BBa_B0012
access: public

BBa_K094014
type: Component
persistent identity: component2056019
display id: component2056019
version: 1
top level: BBa_K283014
definition: BBa_K094014
access: public

BBa_K094142
type: Component
persistent identity: component2056018
display id: component2056018
version: 1
top level: BBa_K283014
definition: BBa_K094142
access: public

BBa_B0034
type: Component
persistent identity: component2056028
display id: component2056028
version: 1
top level: BBa_K283014
definition: BBa_B0034
access: public

BBa_R0010
type: Component
persistent identity: component2056020
display id: component2056020
version: 1
top level: BBa_K283014
definition: LacI
access: public

BBa_B0034
type: Component
persistent identity: component2056016
display id: component2056016
version: 1
top level: BBa_K283014
definition: BBa_B0034
access: public

BBa_K094140
type: Component
persistent identity: component2056014
display id: component2056014
version: 1
top level: BBa_K283014
definition: BBa_K094140
access: public

BBa_B0010
type: Component
persistent identity: component2056033
display id: component2056033
version: 1
top level: BBa_K283014
definition: BBa_B0010
access: public

BBa_K283008
type: Component
persistent identity: component2056032
display id: component2056032
version: 1
top level: BBa_K283014
definition: BBa_K283008
access: public

BBa_K094140
type: SequenceAnnotation
persistent identity: annotation2056014
display id: annotation2056014
version: 1
top level: BBa_K283014
location: https://synbiohub.org/public/igem/BBa_K283014/annotation2056014/range2056014/1
component: BBa_K094140

https://synbiohub.org/public/igem/BBa_K283014/annotation2056014/range2056014/1
type: Range
persistent identity: range2056014
display id: range2056014
version: 1
top level: BBa_K283014
direction: direction/reverse
start: 1
end: 80
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2056016
display id: annotation2056016
version: 1
top level: BBa_K283014
location: https://synbiohub.org/public/igem/BBa_K283014/annotation2056016/range2056016/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K283014/annotation2056016/range2056016/1
type: Range
persistent identity: range2056016
display id: range2056016
version: 1
top level: BBa_K283014
direction: direction/reverse
start: 89
end: 100
orientation: inline

BBa_K094142
type: SequenceAnnotation
persistent identity: annotation2056018
display id: annotation2056018
version: 1
top level: BBa_K283014
location: https://synbiohub.org/public/igem/BBa_K283014/annotation2056018/range2056018/1
component: BBa_K094142

https://synbiohub.org/public/igem/BBa_K283014/annotation2056018/range2056018/1
type: Range
persistent identity: range2056018
display id: range2056018
version: 1
top level: BBa_K283014
direction: direction/reverse
start: 107
end: 1154
orientation: inline

BBa_K094014
type: SequenceAnnotation
persistent identity: annotation2056019
display id: annotation2056019
version: 1
top level: BBa_K283014
location: https://synbiohub.org/public/igem/BBa_K283014/annotation2056019/range2056019/1
component: BBa_K094014

https://synbiohub.org/public/igem/BBa_K283014/annotation2056019/range2056019/1
type: Range
persistent identity: range2056019
display id: range2056019
version: 1
top level: BBa_K283014
direction: direction/reverse
start: 1163
end: 1316
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2056020
display id: annotation2056020
version: 1
top level: BBa_K283014
location: https://synbiohub.org/public/igem/BBa_K283014/annotation2056020/range2056020/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K283014/annotation2056020/range2056020/1
type: Range
persistent identity: range2056020
display id: range2056020
version: 1
top level: BBa_K283014
direction: direction/forward
start: 1325
end: 1524
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2056028
display id: annotation2056028
version: 1
top level: BBa_K283014
location: https://synbiohub.org/public/igem/BBa_K283014/annotation2056028/range2056028/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K283014/annotation2056028/range2056028/1
type: Range
persistent identity: range2056028
display id: range2056028
version: 1
top level: BBa_K283014
direction: direction/reverse
start: 1533
end: 1544
orientation: inline

BBa_K283008
type: SequenceAnnotation
persistent identity: annotation2056032
display id: annotation2056032
version: 1
top level: BBa_K283014
location: https://synbiohub.org/public/igem/BBa_K283014/annotation2056032/range2056032/1
component: BBa_K283008

https://synbiohub.org/public/igem/BBa_K283014/annotation2056032/range2056032/1
type: Range
persistent identity: range2056032
display id: range2056032
version: 1
top level: BBa_K283014
direction: direction/reverse
start: 1551
end: 2263
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2056033
display id: annotation2056033
version: 1
top level: BBa_K283014
location: https://synbiohub.org/public/igem/BBa_K283014/annotation2056033/range2056033/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K283014/annotation2056033/range2056033/1
type: Range
persistent identity: range2056033
display id: range2056033
version: 1
top level: BBa_K283014
direction: direction/forward
start: 2272
end: 2351
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2056035
display id: annotation2056035
version: 1
top level: BBa_K283014
location: https://synbiohub.org/public/igem/BBa_K283014/annotation2056035/range2056035/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K283014/annotation2056035/range2056035/1
type: Range
persistent identity: range2056035
display id: range2056035
version: 1
top level: BBa_K283014
direction: direction/forward
start: 2360
end: 2400
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa R0010
display id: BBa_R0010
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lacI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_R0010
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacI binding site
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0010_sequence/1

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1961221
display id: annotation1961221
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
type: Range
persistent identity: range1961221
display id: range1961221
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 88
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1961222
display id: annotation1961222
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
type: Range
persistent identity: range1961222
display id: range1961222
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 200
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1961223
display id: annotation1961223
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
type: Range
persistent identity: range1961223
display id: range1961223
version: 1
top level: LacI
direction: direction/forward
start: 89
end: 126
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961224
display id: annotation1961224
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
type: Range
persistent identity: range1961224
display id: range1961224
version: 1
top level: LacI
direction: direction/forward
start: 137
end: 142
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1961225
display id: annotation1961225
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
type: Range
persistent identity: range1961225
display id: range1961225
version: 1
top level: LacI
direction: direction/forward
start: 161
end: 166
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1961226
display id: annotation1961226
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
type: Range
persistent identity: range1961226
display id: range1961226
version: 1
top level: LacI
direction: direction/forward
start: 166
end: 200
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1961227
display id: annotation1961227
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
type: Range
persistent identity: range1961227
display id: range1961227
version: 1
top level: LacI
direction: direction/forward
start: 173
end: 173
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_K094014
type: ComponentDefinition
persistent identity: BBa K094014
display id: BBa_K094014
version: 1
was generated by: iGEM to SBOL conversion
description: terminator
created: 2008-08-20T11:00:00Z
modified: 2015-05-08T01:08:40Z
mutable provenance: BBa_K094013
owned by: james
owned by: myers
mutable description: BBa_K094013
discontinued: false
dominant: false
experience: experience/Works
group u list: _255_
m user id: 0
owner id: 2053
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: BBa_K094013
star: false
creator: Shi Lei
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence: https://synbiohub.org/public/igem/BBa_K094014_sequence/1

BBa_K094140
type: ComponentDefinition
persistent identity: BBa K094140
display id: BBa_K094140
version: 1
was generated by: iGEM to SBOL conversion
description: pLacIq
created: 2008-10-12T11:00:00Z
modified: 2015-05-08T01:08:40Z
mutable provenance: point mutation made from E. Coli genomic lacI gene promoter
owned by: james
owned by: myers
mutable description: mutant promoter of lacI gene, which enables high level of lacI protein expression
discontinued: false
dominant: false
experience: experience/Works
group u list: _255_
m user id: 0
owner id: 2589
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: no specific considerations
star: false
creator: Liu Chenli
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: pLacIq
sequence: https://synbiohub.org/public/igem/BBa_K094140_sequence/1

pLacIq
type: SequenceAnnotation
persistent identity: annotation1981123
display id: annotation1981123
version: 1
top level: BBa_K094140
location: https://synbiohub.org/public/igem/BBa_K094140/annotation1981123/range1981123/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K094140/annotation1981123/range1981123/1
type: Range
persistent identity: range1981123
display id: range1981123
version: 1
top level: BBa_K094140
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_K094142
type: ComponentDefinition
persistent identity: BBa K094142
display id: BBa_K094142
version: 1
was generated by: iGEM to SBOL conversion
description: LacIq
created: 2008-10-12T11:00:00Z
modified: 2015-05-08T01:08:40Z
mutable provenance: composited from BBa_K094120 and BBa_K094141
owned by: james
owned by: myers
mutable description: This part is composited with a lacI operon and an mRFP operon. The LacI operon contains a mutated lacIq promoter that enables high level of transcription. The mRFP operon is spontaneously repressed by the LacI operon, and can be turned on by inducing with IPTG.
discontinued: false
dominant: false
experience: experience/Works
group u list: _255_
m user id: 0
owner id: 2589
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: N/A
star: false
creator: Shi Lei
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: protein
sequence: https://synbiohub.org/public/igem/BBa_K094142_sequence/1

protein
type: SequenceAnnotation
persistent identity: annotation1985831
display id: annotation1985831
version: 1
top level: BBa_K094142
location: https://synbiohub.org/public/igem/BBa_K094142/annotation1985831/range1985831/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K094142/annotation1985831/range1985831/1
type: Range
persistent identity: range1985831
display id: range1985831
version: 1
top level: BBa_K094142
direction: direction/forward
start: 1
end: 1048
orientation: inline

BBa_K283008
type: ComponentDefinition
persistent identity: BBa K283008
display id: BBa_K283008
version: 1
was generated by: iGEM to SBOL conversion
description: chez_Histag
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:47Z
mutable provenance: self-designed
owned by: james
owned by: myers
mutable description: This part consists of a chez coding region followed by a 6-Histidine tail
discontinued: false
dominant: false
experience: experience/Works
group u list: _383_
m user id: 0
owner id: 5494
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: BBa_K283008
star: false
creator: W Ling , Nelson Chu, Shi Lei
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_K094100
sequence annotation: cheZ
sequence annotation: 6-His tag
sequence: https://synbiohub.org/public/igem/BBa_K283008_sequence/1

BBa_K094100
type: SequenceAnnotation
persistent identity: annotation2035290
display id: annotation2035290
version: 1
top level: BBa_K283008
location: https://synbiohub.org/public/igem/BBa_K283008/annotation2035290/range2035290/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K283008/annotation2035290/range2035290/1
type: Range
persistent identity: range2035290
display id: range2035290
version: 1
top level: BBa_K283008
direction: direction/reverse
start: 1
end: 695
orientation: inline

cheZ
type: SequenceAnnotation
persistent identity: annotation2035289
display id: annotation2035289
version: 1
top level: BBa_K283008
location: https://synbiohub.org/public/igem/BBa_K283008/annotation2035289/range2035289/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_K283008/annotation2035289/range2035289/1
type: Range
persistent identity: range2035289
display id: range2035289
version: 1
top level: BBa_K283008
direction: direction/forward
start: 1
end: 645
orientation: inline

6-His tag
type: SequenceAnnotation
persistent identity: annotation2035366
display id: annotation2035366
version: 1
top level: BBa_K283008
location: https://synbiohub.org/public/igem/BBa_K283008/annotation2035366/range2035366/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_K283008/annotation2035366/range2035366/1
type: Range
persistent identity: range2035366
display id: range2035366
version: 1
top level: BBa_K283008
direction: direction/reverse
start: 696
end: 713
orientation: inline

https://synbiohub.org/public/igem/BBa_K283014_sequence/1
type: Sequence
persistent identity: BBa K283014 sequence
display id: BBa_K283014_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacaccatcgaatggtgcaaaacctttcgcggtatggcatgatagcgcccggaagagagtcaattcagggtggtgaattactagagaaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatactagagtgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgatgcaaccatcaatcaaacctgctgacgagcattcagctggcgatatcattgcgcgcatcggcagcctgacgcgtatgctgcgcgacagtttgcgggaactggggctggatcaggccattgccgaagcggcggaagccatccccgatgcgcgcgatcgtttgtactatgttgtgcagatgaccgcccaggctgcggagcgggcgctgaacagtgttgaggcgtcacaaccgcatcaggatcaaatggagaaatcagcaaaagcgttaacccaacgttgggatgactggtttgccgatccgattgaccttgccgacgcccgtgaactggtaacagatacacgacaatttctggcagatgtacccgcgcataccagctttactaacgcgcaactgctggaaatcatgatggcgcaggattttcaggatctcaccgggcaggtcattaag…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0010_sequence/1
type: Sequence
persistent identity: BBa R0010 sequence
display id: BBa_R0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K094014_sequence/1
type: Sequence
persistent identity: BBa K094014 sequence
display id: BBa_K094014_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K094140_sequence/1
type: Sequence
persistent identity: BBa K094140 sequence
display id: BBa_K094140_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacaccatcgaatggtgcaaaacctttcgcggtatggcatgatagcgcccggaagagagtcaattcagggtggtgaat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K094142_sequence/1
type: Sequence
persistent identity: BBa K094142 sequence
display id: BBa_K094142_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K283008_sequence/1
type: Sequence
persistent identity: BBa K283008 sequence
display id: BBa_K283008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgatgcaaccatcaatcaaacctgctgacgagcattcagctggcgatatcattgcgcgcatcggcagcctgacgcgtatgctgcgcgacagtttgcgggaactggggctggatcaggccattgccgaagcggcggaagccatccccgatgcgcgcgatcgtttgtactatgttgtgcagatgaccgcccaggctgcggagcgggcgctgaacagtgttgaggcgtcacaaccgcatcaggatcaaatggagaaatcagcaaaagcgttaacccaacgttgggatgactggtttgccgatccgattgaccttgccgacgcccgtgaactggtaacagatacacgacaatttctggcagatgtacccgcgcataccagctttactaacgcgcaactgctggaaatcatgatggcgcaggattttcaggatctcaccgggcaggtcattaagcggatgatggatgtcattcaggagatcgaacgccagttgctgatggtgctgttggaaaacatcccggaacaggagtcgcgtccaaaacgtgaaaaccagagtttgcttaatggacctcaggtcgataccagcaaagccggtgtggtagccagtcaggatcaggtggacgatttgttggatagtcttggattttgagctagcaacgcaatgacgtcggaattgccagctggggcgccctctggtaacatcatcaccatcaccac
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z