LOCUS       BBa_K283016             1076 bp    DNA     linear       26-Nov-2024
DEFINITION  pLacI-rbs-cheZ_histag-terminators
ACCESSION   BBa_K283016
VERSION     BBa_K283016.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             227..871
                     /label=cheZ
     misc_feature    227..921
                     /label=BBa_K094100
     misc_feature    922..939
                     /label=6-His tag
     CDS             227..939
                     /label=BBa_K283008
     stem_loop       959..1002
                     /label=stem_loop
     misc_feature    948..1027
                     /label=BBa_B0010
     terminator      948..1027
                     /label=BBa_B0010
     stem_loop       1043..1062
                     /label=T7 TE
     misc_feature    1069..1069
                     /label=stop
     polyA_site      1063..1076
                     /label=polya
     misc_feature    1036..1076
                     /label=BBa_B0012
     terminator      1036..1076
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga tgcaaccatc
      241 aatcaaacct gctgacgagc attcagctgg cgatatcatt gcgcgcatcg gcagcctgac
      301 gcgtatgctg cgcgacagtt tgcgggaact ggggctggat caggccattg ccgaagcggc
      361 ggaagccatc cccgatgcgc gcgatcgttt gtactatgtt gtgcagatga ccgcccaggc
      421 tgcggagcgg gcgctgaaca gtgttgaggc gtcacaaccg catcaggatc aaatggagaa
      481 atcagcaaaa gcgttaaccc aacgttggga tgactggttt gccgatccga ttgaccttgc
      541 cgacgcccgt gaactggtaa cagatacacg acaatttctg gcagatgtac ccgcgcatac
      601 cagctttact aacgcgcaac tgctggaaat catgatggcg caggattttc aggatctcac
      661 cgggcaggtc attaagcgga tgatggatgt cattcaggag atcgaacgcc agttgctgat
      721 ggtgctgttg gaaaacatcc cggaacagga gtcgcgtcca aaacgtgaaa accagagttt
      781 gcttaatgga cctcaggtcg ataccagcaa agccggtgtg gtagccagtc aggatcaggt
      841 ggacgatttg ttggatagtc ttggattttg agctagcaac gcaatgacgt cggaattgcc
      901 agctggggcg ccctctggta acatcatcac catcaccact actagagcca ggcatcaaat
      961 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
     1021 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata
//