##gff-version 3 ##sequence-region BBa_K283016 1 1076 BBa_K283016 . ribosome_entry_site 209 220 . + 0 ID=BBa_B0034;Name=BBa_B0034 BBa_K283016 . conserved_region 213 216 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_K283016 . CDS 227 939 . + 0 ID=BBa_K283008;Name=BBa_K283008 BBa_K283016 . tag 922 939 . + 0 ID=annotation2035366;Name=6-His tag;Parent=BBa_K283008 BBa_K283016 . CDS 227 871 . + 0 ID=annotation2035289;Name=cheZ;Parent=BBa_K283008 BBa_K283016 . engineered_region 227 921 . + 0 ID=annotation2035290;Name=BBa_K094100;Parent=BBa_K283008 BBa_K283016 . terminator 1036 1076 . + 0 ID=BBa_B0012;Name=BBa_B0012 BBa_K283016 . stem_loop 1043 1062 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_K283016 . engineered_region 1036 1076 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_K283016 . stop_codon 1069 1069 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_K283016 . polyA_site 1063 1076 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_K283016 . promoter 1 200 . + 0 ID=BBa_R0010;Name=BBa_R0010 BBa_K283016 . engineered_region 1 200 . + 0 ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010 BBa_K283016 . non_covalent_binding_site 166 200 . + 0 ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010 BBa_K283016 . CDS 1 88 . + 0 ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010 BBa_K283016 . non_covalent_binding_site 89 126 . + 0 ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010 BBa_K283016 . promoter 137 142 . + 0 ID=annotation1961224;Name=-35;Parent=BBa_R0010 BBa_K283016 . start_codon 173 173 . + 0 ID=annotation1961227;Name=start;Parent=BBa_R0010 BBa_K283016 . promoter 161 166 . + 0 ID=annotation1961225;Name=-10;Parent=BBa_R0010 BBa_K283016 . terminator 948 1027 . + 0 ID=BBa_B0010;Name=BBa_B0010 BBa_K283016 . engineered_region 948 1027 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_K283016 . stem_loop 959 1002 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 >BBa_K283016 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgatgcaacc atcaatcaaacctgctgacgagcattcagctggcgatatcattgcgcgcatcggcagcctgacgcgtatgctgcgcgac agtttgcgggaactggggctggatcaggccattgccgaagcggcggaagccatccccgatgcgcgcgatcgtttgtact atgttgtgcagatgaccgcccaggctgcggagcgggcgctgaacagtgttgaggcgtcacaaccgcatcaggatcaaat ggagaaatcagcaaaagcgttaacccaacgttgggatgactggtttgccgatccgattgaccttgccgacgcccgtgaa ctggtaacagatacacgacaatttctggcagatgtacccgcgcataccagctttactaacgcgcaactgctggaaatca tgatggcgcaggattttcaggatctcaccgggcaggtcattaagcggatgatggatgtcattcaggagatcgaacgcca gttgctgatggtgctgttggaaaacatcccggaacaggagtcgcgtccaaaacgtgaaaaccagagtttgcttaatgga cctcaggtcgataccagcaaagccggtgtggtagccagtcaggatcaggtggacgatttgttggatagtcttggatttt gagctagcaacgcaatgacgtcggaattgccagctggggcgccctctggtaacatcatcaccatcaccactactagagc caggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc tactagagtcacactggctcaccttcgggtgggcctttctgcgtttata