LOCUS       BBa_K283019             1027 bp    DNA     linear       18-Dec-2024
DEFINITION  FF_FF ansB promoter-rbs- GFP (LVA)- terminators
ACCESSION   BBa_K283019
VERSION     BBa_K283019.1  
FEATURES             Location/Qualifiers
     promoter        1..105
                     /label=promoter
     misc_feature    1..24
                     /label=FNR
     misc_feature    53..74
                     /label=FNR
     promoter        1..105
                     /label=BBa_K283031
     misc_feature    118..121
                     /label=conserved
     RBS             114..125
                     /label=BBa_B0034
     misc_feature    132..851
                     /label=BBa_E0040
     misc_feature    846..884
                     /label=LVA
     misc_feature    885..890
                     /label=Double Stop
     misc_feature    132..134
                     /label=Start
     misc_feature    132..890
                     /label=BBa_J04031
     stem_loop       910..953
                     /label=stem_loop
     misc_feature    899..978
                     /label=BBa_B0010
     terminator      899..978
                     /label=BBa_B0010
     stem_loop       994..1013
                     /label=T7 TE
     misc_feature    1020..1020
                     /label=stop
     polyA_site      1014..1027
                     /label=polya
     misc_feature    987..1027
                     /label=BBa_B0012
     terminator      987..1027
                     /label=BBa_B0012
ORIGIN
        1 ttttttgtta cctgcctcta actttgtaga tctccaaaat atattcacgt tgtaaattgt
       61 ttaacgtcaa atttcccata cagagctaag ggataatgcg tagcgtacta gagaaagagg
      121 agaaatacta gatgcgtaaa ggagaagaac ttttcactgg agttgtccca attcttgttg
      181 aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg
      241 caacatacgg aaaacttacc cttaaattta tttgcactac tggaaaacta cctgttccat
      301 ggccaacact tgtcactact ttcggttatg gtgttcaatg ctttgcgaga tacccagatc
      361 atatgaaaca gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaaagaa
      421 ctatattttt caaagatgac gggaactaca agacacgtgc tgaagtcaag tttgaaggtg
      481 atacccttgt taatagaatc gagttaaaag gtattgattt taaagaagat ggaaacattc
      541 ttggacacaa attggaatac aactataact cacacaatgt atacatcatg gcagacaaac
      601 aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa cattgaagat ggaagcgttc
      661 aactagcaga ccattatcaa caaaatactc caattggcga tggccctgtc cttttaccag
      721 acaaccatta cctgtccaca caatctgccc tttcgaaaga tcccaacgaa aagagagacc
      781 acatggtcct tcttgagttt gtaacagctg ctgggattac acatggcatg gatgaactat
      841 acaaaaggcc tgctgcaaac gacgaaaact acgctttagt agcttaataa tactagagcc
      901 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
      961 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc
     1021 gtttata
//