LOCUS       BBa_K283020              981 bp    DNA     linear       19-Dec-2024
DEFINITION  FF_FF ansB promoter-rbs-cheZ_histag-terminators
ACCESSION   BBa_K283020
VERSION     BBa_K283020.1  
FEATURES             Location/Qualifiers
     promoter        1..105
                     /label=promoter
     misc_feature    1..24
                     /label=FNR
     misc_feature    53..74
                     /label=FNR
     promoter        1..105
                     /label=BBa_K283031
     misc_feature    118..121
                     /label=conserved
     RBS             114..125
                     /label=BBa_B0034
     CDS             132..776
                     /label=cheZ
     misc_feature    132..826
                     /label=BBa_K094100
     misc_feature    827..844
                     /label=6-His tag
     CDS             132..844
                     /label=BBa_K283008
     stem_loop       864..907
                     /label=stem_loop
     misc_feature    853..932
                     /label=BBa_B0010
     terminator      853..932
                     /label=BBa_B0010
     stem_loop       948..967
                     /label=T7 TE
     misc_feature    974..974
                     /label=stop
     polyA_site      968..981
                     /label=polya
     misc_feature    941..981
                     /label=BBa_B0012
     terminator      941..981
                     /label=BBa_B0012
ORIGIN
        1 ttttttgtta cctgcctcta actttgtaga tctccaaaat atattcacgt tgtaaattgt
       61 ttaacgtcaa atttcccata cagagctaag ggataatgcg tagcgtacta gagaaagagg
      121 agaaatacta gatgatgcaa ccatcaatca aacctgctga cgagcattca gctggcgata
      181 tcattgcgcg catcggcagc ctgacgcgta tgctgcgcga cagtttgcgg gaactggggc
      241 tggatcaggc cattgccgaa gcggcggaag ccatccccga tgcgcgcgat cgtttgtact
      301 atgttgtgca gatgaccgcc caggctgcgg agcgggcgct gaacagtgtt gaggcgtcac
      361 aaccgcatca ggatcaaatg gagaaatcag caaaagcgtt aacccaacgt tgggatgact
      421 ggtttgccga tccgattgac cttgccgacg cccgtgaact ggtaacagat acacgacaat
      481 ttctggcaga tgtacccgcg cataccagct ttactaacgc gcaactgctg gaaatcatga
      541 tggcgcagga ttttcaggat ctcaccgggc aggtcattaa gcggatgatg gatgtcattc
      601 aggagatcga acgccagttg ctgatggtgc tgttggaaaa catcccggaa caggagtcgc
      661 gtccaaaacg tgaaaaccag agtttgctta atggacctca ggtcgatacc agcaaagccg
      721 gtgtggtagc cagtcaggat caggtggacg atttgttgga tagtcttgga ttttgagcta
      781 gcaacgcaat gacgtcggaa ttgccagctg gggcgccctc tggtaacatc atcaccatca
      841 ccactactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
      901 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg
      961 gtgggccttt ctgcgtttat a
//