LOCUS       BBa_K283021              912 bp    DNA     linear       14-Jun-2024
DEFINITION  pTet-rbs-cheZ-termintors
ACCESSION   BBa_K283021
VERSION     BBa_K283021.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..725
                     /label=cheZ
     CDS             81..775
                     /label=BBa_K094100
     stem_loop       795..838
                     /label=stem_loop
     misc_feature    784..863
                     /label=BBa_B0010
     terminator      784..863
                     /label=BBa_B0010
     stem_loop       879..898
                     /label=T7 TE
     misc_feature    905..905
                     /label=stop
     polyA_site      899..912
                     /label=polya
     misc_feature    872..912
                     /label=BBa_B0012
     terminator      872..912
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgatgcaac catcaatcaa acctgctgac gagcattcag
      121 ctggcgatat cattgcgcgc atcggcagcc tgacgcgtat gctgcgcgac agtttgcggg
      181 aactggggct ggatcaggcc attgccgaag cggcggaagc catccccgat gcgcgcgatc
      241 gtttgtacta tgttgtgcag atgaccgccc aggctgcgga gcgggcgctg aacagtgttg
      301 aggcgtcaca accgcatcag gatcaaatgg agaaatcagc aaaagcgtta acccaacgtt
      361 gggatgactg gtttgccgat ccgattgacc ttgccgacgc ccgtgaactg gtaacagata
      421 cacgacaatt tctggcagat gtacccgcgc ataccagctt tactaacgcg caactgctgg
      481 aaatcatgat ggcgcaggat tttcaggatc tcaccgggca ggtcattaag cggatgatgg
      541 atgtcattca ggagatcgaa cgccagttgc tgatggtgct gttggaaaac atcccggaac
      601 aggagtcgcg tccaaaacgt gaaaaccaga gtttgcttaa tggacctcag gtcgatacca
      661 gcaaagccgg tgtggtagcc agtcaggatc aggtggacga tttgttggat agtcttggat
      721 tttgagctag caacgcaatg acgtcggaat tgccagctgg ggcgccctct ggtaatacta
      781 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
      841 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt
      901 tctgcgttta ta
//