LOCUS       BBa_K283023             2623 bp    DNA     linear       22-Nov-2024
DEFINITION  plux-rbs-CI-terminator-plambda(R-O12)-rbs-cheZ-terminator
ACCESSION   BBa_K283023
VERSION     BBa_K283023.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=LuxR/HSL
     promoter        20..25
                     /label=-35
     promoter        42..47
                     /label=-10
     misc_feature    53..53
                     /label=start
     misc_feature    1..55
                     /label=BBa_R0062
     promoter        1..55
                     /label=BBa_R0062
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     CDS             82..1590
                     /label=BBa_K094011
     terminator      1599..1692
                     /label=BBa_K094013
     promoter        1701..1800
                     /label=promoter
     promoter        1701..1800
                     /label=BBa_K094002
     misc_feature    1813..1816
                     /label=conserved
     RBS             1809..1820
                     /label=BBa_B0034
     CDS             1827..2471
                     /label=cheZ
     CDS             1827..2521
                     /label=BBa_K094100
     terminator      2530..2623
                     /label=BBa_K094013
ORIGIN
        1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ataaatacta
       61 gagaaagagg agaaatacta gatgagcaca aaaaagaaac cattaacaca agagcagctt
      121 gaggacgcac gtcgccttaa agcaatttat gaaaaaaaga aaaatgaact tggcttatcc
      181 caggaatctg tcgcagacaa gatggggatg gggcagtcag gcgttggtgc tttatttaat
      241 ggcatcaatg cattaaatgc ttataacgcc gcattgcttg caaaaattct caaagttagc
      301 gttgaagaat tcagcccttc aatcgccaga gagatctacg agatgtatga agcggttagt
      361 atgcagccgt cacttagaag tgagtatgag taccctgttt tttctcatgt tcaggcaggg
      421 atgttctcac ctgagcttag aacctttacc aaaggtgatg cggagagatg ggtaagcaca
      481 accaaaaaag ccagtgattc tgcattctgg cttgaggttg aaggtaattc catgaccgca
      541 ccaacaggct ccaagccaag ctttcctgac ggaatgttaa ttctcgttga ccctgagcag
      601 gctgttgagc ccggggattt ctgcatagcc agacttgggg gtgatgagtt taccttcaag
      661 aaactgatca gggatagcgg tcaggtgttt ttacaaccac taaacccaca gtacccaatg
      721 atcccatgca atgagagttg ttccgttgtg gggaaagtta tcgctagtca gtggcctgaa
      781 gagacgtttg gctgatcggc aaggtgttct ggtcggcgca tagctgataa caattggtac
      841 ctagaattgc ttaaagagga gaaattaagc atggtgagca agggcgagga gctgttcacc
      901 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
      961 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
     1021 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgacctg gggcgtgcag
     1081 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     1141 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
     1201 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     1261 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacat cagccacaac
     1321 gtctatatca ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa gatccgccac
     1381 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
     1441 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa
     1501 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     1561 actctcggca tggacgagct gtacaagtaa tactagagaa cgaaaggctc agtcgaaaga
     1621 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc
     1681 gccgccctag actactagag ctagtagcac gacaggttcc tagtaaatat ctaacaccgt
     1741 gcgtgttgac tattttacct ctggcggtga taatggttgc atgtagctac cgaattcagc
     1801 tactagagaa agaggagaaa tactagatga tgcaaccatc aatcaaacct gctgacgagc
     1861 attcagctgg cgatatcatt gcgcgcatcg gcagcctgac gcgtatgctg cgcgacagtt
     1921 tgcgggaact ggggctggat caggccattg ccgaagcggc ggaagccatc cccgatgcgc
     1981 gcgatcgttt gtactatgtt gtgcagatga ccgcccaggc tgcggagcgg gcgctgaaca
     2041 gtgttgaggc gtcacaaccg catcaggatc aaatggagaa atcagcaaaa gcgttaaccc
     2101 aacgttggga tgactggttt gccgatccga ttgaccttgc cgacgcccgt gaactggtaa
     2161 cagatacacg acaatttctg gcagatgtac ccgcgcatac cagctttact aacgcgcaac
     2221 tgctggaaat catgatggcg caggattttc aggatctcac cgggcaggtc attaagcgga
     2281 tgatggatgt cattcaggag atcgaacgcc agttgctgat ggtgctgttg gaaaacatcc
     2341 cggaacagga gtcgcgtcca aaacgtgaaa accagagttt gcttaatgga cctcaggtcg
     2401 ataccagcaa agccggtgtg gtagccagtc aggatcaggt ggacgatttg ttggatagtc
     2461 ttggattttg agctagcaac gcaatgacgt cggaattgcc agctggggcg ccctctggta
     2521 atactagaga acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
     2581 cggtgaacgc tctcctgagt aggacaaatc cgccgcccta gac
//