LOCUS       BBa_K283027              914 bp    DNA     linear       18-Dec-2024
DEFINITION  rbs-GFP(LVA)-terminators (Backbone: pSB1AK3)
ACCESSION   BBa_K283027
VERSION     BBa_K283027.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     misc_feature    19..738
                     /label=BBa_E0040
     misc_feature    733..771
                     /label=LVA
     misc_feature    772..777
                     /label=Double Stop
     misc_feature    19..21
                     /label=Start
     misc_feature    19..777
                     /label=BBa_J04031
     stem_loop       797..840
                     /label=stem_loop
     misc_feature    786..865
                     /label=BBa_B0010
     terminator      786..865
                     /label=BBa_B0010
     stem_loop       881..900
                     /label=T7 TE
     misc_feature    907..907
                     /label=stop
     polyA_site      901..914
                     /label=polya
     misc_feature    874..914
                     /label=BBa_B0012
     terminator      874..914
                     /label=BBa_B0012
ORIGIN
        1 aaagaggaga aatactagat gcgtaaagga gaagaacttt tcactggagt tgtcccaatt
       61 cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa
      121 ggtgatgcaa catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct
      181 gttccatggc caacacttgt cactactttc ggttatggtg ttcaatgctt tgcgagatac
      241 ccagatcata tgaaacagca tgactttttc aagagtgcca tgcccgaagg ttatgtacag
      301 gaaagaacta tatttttcaa agatgacggg aactacaaga cacgtgctga agtcaagttt
      361 gaaggtgata cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga
      421 aacattcttg gacacaaatt ggaatacaac tataactcac acaatgtata catcatggca
      481 gacaaacaaa agaatggaat caaagttaac ttcaaaatta gacacaacat tgaagatgga
      541 agcgttcaac tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt
      601 ttaccagaca accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag
      661 agagaccaca tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat
      721 gaactataca aaaggcctgc tgcaaacgac gaaaactacg ctttagtagc ttaataatac
      781 tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat
      841 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc
      901 tttctgcgtt tata
//