LOCUS BBa_K283045 3178 bp DNA linear 22-Dec-2024 DEFINITION prosseA-rbs- T7 RNAP (Backbone: pSB3C5) ACCESSION BBa_K283045 VERSION BBa_K283045.1 FEATURES Location/Qualifiers promoter 1..500 /label=prosseA promoter 1..500 /label=BBa_K283038 RBS 509..520 /label=BBa_J61134 CDS 527..3178 /label=T7 polymerase CDS 527..3178 /label=BBa_K283047 ORIGIN 1 actttgaccc ggttagaaga tttgctgctt cattcgcgtg aagaggccaa aggcataatt 61 ttacaattaa gggctgcccg gaaacagtta gaagagaaca acggcaagtt acaggatccg 121 cagcaatatc agcaaaacac cttattgctt gaagcgatcg agcaggccga aaatatcatc 181 aacattattt attatcgtta ccataacagc gcacttgtag tgagtgagca agagtaaagt 241 aaaaatatct tagagcctat cccaccaggc gttaattggc gcagccagtt tggacacgga 301 tagcgcgcaa aaaccgcagc gtacacgtag tacgtgaggt ttgactcgct acgctcgccc 361 ttcgggccgc cgctagcggc gttcaaaacg ctaacgcgtt ttggcgagca ctgcccaggt 421 tcaaaatggc aagtaaaata gccctaatgg gataggctct tagttagcac gttaattatc 481 tatcgtgtat atggagggga tactagagaa agaccggaaa tactagatga acacgattaa 541 catcgctaag aacgacttct ctgacatcga actggctgct atcccgttca acactctggc 601 tgaccattac ggtgagcgtt tagctcgcga acagttggcc cttgagcatg agtcttacga 661 gatgggtgaa gcacgcttcc gcaagatgtt tgagcgtcaa cttaaagctg gtgaggttgc 721 ggataacgct gccgccaagc ctctcatcac taccctactc cctaagatga ttgcacgcat 781 caacgactgg tttgaggaag tgaaagctaa gcgcggcaag cgcccgacag ccttccagtt 841 cctgcaagaa atcaagccgg aagccgtagc gtacatcacc attaagacca ctctggcttg 901 cctaaccagt gctgacaata caaccgttca ggctgtagca agcgcaatcg gtcgggccat 961 tgaggacgag gctcgcttcg gtcgtatccg tgaccttgaa gctaagcact tcaagaaaaa 1021 cgttgaggaa caactcaaca agcgcgtagg gcacgtctac aagaaagcat ttatgcaagt 1081 tgtcgaggct gacatgctct ctaagggtct actcggtggc gaggcgtggt cttcgtggca 1141 taaggaagac tctattcatg taggagtacg ctgcatcgag atgctcattg agtcaaccgg 1201 aatggttagc ttacaccgcc aaaatgctgg cgtagtaggt caagactctg agactatcga 1261 actcgcacct gaatacgctg aggctatcgc aacccgtgca ggtgcgctgg ctggcatctc 1321 tccgatgttc caaccttgcg tagttcctcc taagccgtgg actggcatta ctggtggtgg 1381 ctattgggct aacggtcgtc gtcctctggc gctggtgcgt actcacagta agaaagcact 1441 gatgcgctac gaagacgttt acatgcctga ggtgtacaaa gcgattaaca ttgcgcaaaa 1501 caccgcatgg aaaatcaaca agaaagtcct agcggtcgcc aacgtaatca ccaagtggaa 1561 gcattgtccg gtcgaggaca tccctgcgat tgagcgtgaa gaactcccga tgaaaccgga 1621 agacatcgac atgaatcctg aggctctcac cgcgtggaaa cgtgctgccg ctgctgtgta 1681 ccgcaaggac agggctcgca agtctcgccg tatcagcctt gagttcatgc ttgagcaagc 1741 caataagttt gctaaccata aggccatctg gttcccttac aacatggact ggcgcggtcg 1801 tgtttacgcc gtgtcaatgt tcaacccgca aggtaacgat atgaccaaag gactgcttac 1861 gctggcgaaa ggtaaaccaa tcggtaagga aggttactac tggctgaaaa tccacggtgc 1921 aaactgtgcg ggtgtcgata aggttccgtt ccctgagcgc atcaagttca ttgaggaaaa 1981 ccacgagaac atcatggctt gcgctaagtc tccactggag aacacttggt gggctgagca 2041 agattctccg ttctgcttcc ttgcgttctg ctttgagtac gctggggtac agcaccacgg 2101 cctgagctat aactgctccc ttccgctggc gtttgacggg tcttgctctg gcatccagca 2161 cttctccgcg atgctccgag atgaggtagg tggtcgcgcg gttaacttgc ttcctagtga 2221 gaccgttcag gacatctacg ggattgttgc taagaaagtc aacgagattc tacaagcaga 2281 cgcaatcaat gggaccgata acgaagtagt taccgtgacc gatgagaaca ctggtgaaat 2341 ctctgagaaa gtcaagctgg gcactaaggc actggctggt caatggctgg ctcacggtgt 2401 tactcgcagt gtgactaagc gttcagtcat gacgctggct tacgggtcca aagagttcgg 2461 cttccgtcaa caagtgctgg aagataccat tcagccagct attgattccg gcaagggtcc 2521 gatgttcact cagccgaatc aggctgctgg atacatggct aagctgattt gggaatctgt 2581 gagcgtgacg gtggtagctg cggttgaagc aatgaactgg cttaagtctg ctgctaagct 2641 gctggctgct gaggtcaaag ataagaagac tggagagatt cttcgcaagc gttgcgctgt 2701 gcattgggta actcctgatg gtttccctgt gtggcaggaa tacaagaagc ctattcagac 2761 gcgcttgaac ctgatgttcc tcggtcagtt ccgcttacag cctaccatta acaccaacaa 2821 agatagcgag attgatgcac acaaacagga gtctggtatc gctcctaact ttgtacacag 2881 ccaagacggt agccaccttc gtaagactgt agtgtgggca cacgagaagt acggaatcga 2941 atcttttgca ctgattcacg actccttcgg taccattccg gctgacgctg cgaacctgtt 3001 caaagcagtg cgcgaaacta tggttgacac atatgagtct tgtgatgtac tggctgattt 3061 ctacgaccag ttcgctgacc agttgcacga gtctcaattg gacaaaatgc cagcacttcc 3121 ggctaaaggt aacttgaacc tccgtgacat cttagagtcg gacttcgcgt tcgcgtaa //