LOCUS       BBa_K284019             3326 bp    DNA     linear       22-Dec-2024
DEFINITION  Lactate Permease constitutive expression
ACCESSION   BBa_K284019
VERSION     BBa_K284019.1  
FEATURES             Location/Qualifiers
     promoter        1..1445
                     /label=Constitutive promoter
     misc_feature    1348..1354
                     /label=TATA Box
     promoter        1..1445
                     /label=BBa_J63005
     primer_bind     1454..1474
                     /label=primer_binding
     CDS             1460..3262
                     /label=Lactate Permease
     misc_feature    1460..1462
                     /label=Start
     misc_feature    3260..3262
                     /label=Stop
     primer_bind     3308..3326
                     /label=primer_binding
     CDS             1454..3326
                     /label=BBa_K284000
ORIGIN
        1 aagaaatgat ggtaaatgaa ataggaaatc aaggagcatg aaggcaaaag acaaatataa
       61 gggtcgaacg aaaaataaag tgaaaagtgt tgatatgatg tatttggctt tgcggcgccg
      121 aaaaaacgag tttacgcaat tgcacaatca tgctgactct gtggcggacc cgcgctcttg
      181 ccggcccggc gataacgctg ggcgtgaggc tgtgcccggc ggagtttttt gcgcctgcat
      241 tttccaaggt ttaccctgcg ctaaggggcg agattggaga agcaataaga atgccggttg
      301 gggttgcgat gatgacgacc acgacaactg gtgtcattat ttaagttgcc gaaagaacct
      361 gagtgcattt gcaacatgag tatactagaa gaatgagcca agacttgcga gacgcgagtt
      421 tgccggtggt gcgaacaata gagcgaccat gaccttgaag gtgagacgcg cataaccgct
      481 agagtacttt gaagaggaaa cagcaatagg gttgctacca gtataaatag acaggtacat
      541 acaacactgg aaatggttgt ctgtttgagt acgctttcaa ttcatttggg tgtgcacttt
      601 attatgttac aatatggaag ggaactttac acttctccta tgcacatata ttaattaaag
      661 tccaatgcta gtagagaagg ggggtaacac ccctccgcgc tcttttccga tttttttcta
      721 aaccgtggaa tatttcggat atccttttgt tgtttccggg tgtacaatat ggacttcctc
      781 ttttctggca accaaaccca tacatcggga ttcctataat accttcgttg gtctccctaa
      841 catgtaggtg gcggagggga gatatacaat agaacagata ccagacaaga cataatgggc
      901 taaacaagac tacaccaatt acactgcctc attgatggtg gtacataacg aactaatact
      961 gtagccctag acttgatagc catcatcata tcgaagtttc actacccttt ttccatttgc
     1021 catctattga agtaataata ggcgcatgca acttcttttc tttttttttc ttttctctct
     1081 cccccgttgt tgtctcacca tatccgcaat gacaaaaaaa atgatggaag acactaaagg
     1141 aaaaaattaa cgacaaagac agcaccaaca gatgtcgttg ttccagagct gatgaggggt
     1201 atcttcgaac acacgaaact ttttccttcc ttcattcacg cacactactc tctaatgagc
     1261 aacggtatac ggccttcctt ccagttactt gaatttgaaa taaaaaaagt ttgccgcttt
     1321 gctatcaagt ataaatagac ctgcaattat taatcttttg tttcctcgtc attgttctcg
     1381 ttccctttct tccttgtttc tttttctgca caatatttca agctatacca agcatacaat
     1441 caacttacta gagtcaacca tgaataacaa caatattaca ccagagtctg actctatgaa
     1501 atctaatgat aatgatcaaa caaacgatta tatgccggat gtagcggatt tcgaccatac
     1561 gcaaaccaat accaatgaga ttgctagagc catttcccat cctggttcag ttttgtcacg
     1621 tgttgcatcg tatgtgtcaa gaaaggacag atatgtcgat gaaaacggta acgaggtgtg
     1681 gcaggacgat gaagtgtcga ttcttatgga ggaagacgaa accccagatt tcacttggaa
     1741 aaacattcgt cactatgcga tcactagatt taccactttg accgagcttc atagggtatc
     1801 gatggaaaat atcaatccga tcccagagtt gagaaagatg actttacaca actggaacta
     1861 tttctttatg gggtatgcgg cttggttatg tgccgcttgg gcgttctttg cagtgtccgt
     1921 ttctacggct ccattggcta ctttgtacgg caaagaaact aaggatatct cttggggtct
     1981 ttcgttggtt cttttcgtta gatctgctgg tgccatcatc ttcggtatct ggaccgataa
     2041 ctattcgaga aaatggcctt acatcacttg tttgggttta ttcttaatct gtcaactttg
     2101 taccccatgg gccaagactt acactcagtt cttgggtgtt agatggattt ctggtatcgc
     2161 tatgggtggt atctacgctt gtgcctctgc cactgccatt gaagatgccc cagtgaaggc
     2221 aagatccttc ctatcaggtc ttttctttac cgcatatgct atgggtttca tctttgccat
     2281 catcttttac agagctttct tgaacgttaa cggtgaaaac tactggaagg ttcaattctg
     2341 gttctctatc tggttaccag ctgtgttgat cttatggaga ttggtttggc ctgagactaa
     2401 atatttcact aaggtgttaa aggctagaca attgatgaga gacgatgcca ttgccaagaa
     2461 cggtggtcaa ccattgccaa aattgtcttt caaacaaaaa ttcgctaacg ttaagaaaac
     2521 tgtttctaaa tactggttat tgttcggtta cttgattctc ttgcttgttg gtccaaacta
     2581 cttgacccat gcctctcaag atttgttccc aaccatgtta cgtgctcaat tgcgtttctc
     2641 tgaagacgct gttaccgttg ccattgttgt tgtatgtttg ggttccatcg ctggtggtat
     2701 gttctttggt caactaatgg aaatcaccgg tagaagagta ggtttgctct tggcccttat
     2761 catggccggt tgtttcactt atccagcttt catgttgaag acaagttctg ctgtgttggg
     2821 tgcagggttc atgctttggt tctccattct tggtgtctgg ggtgttcttc caatccatct
     2881 ttccgaatta tctcctccag aagctagagc tttggtctct ggtcttgcct atcaattagg
     2941 taacttggct tctgccgctt cggtcgttat cgaaaacgat ttggctgatt tgtacccaat
     3001 cgaatggaac tccgcaggtg aagtaacaaa caaggattac tctaaagtta tggctatctt
     3061 gactggttcc agtgtcatct tcacttttgt cttggtattt gtcggtcacg agaagttcca
     3121 cagagattta tcctctcctc atttgaaatc ttatatcgaa agagttgacc aaacggaaga
     3181 agttgctgct atgacaggtt ccacggccaa ttctatttct tctaagccaa gtgatgatca
     3241 attggaaaag gtttcagttt gattaatgat taacagaaac taaaccttta tacaatttca
     3301 tgcccacaca cgcaccatag cattat
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