BBa_K284020 1 BBa_K284020 EYFP regulated by lactate responsive promoter (JEN1) 2009-10-11T11:00:00Z 2015-05-08T01:11:48Z . . false false _386_ 0 4968 9 Not in stock false . false Gleidson Silva Teixeira component2244293 1 BBa_E2030 component2244288 1 BBa_K284002 annotation2244288 1 BBa_K284002 range2244288 1 1 998 annotation2244293 1 BBa_E2030 range2244293 1 1005 1782 BBa_K284002 1 BBa_K284002 JEN1 Promoter from Kluyveromyces lactis 2009-09-25T11:00:00Z 2015-05-08T01:11:48Z . . false false _386_ 0 4968 9 It's complicated true . false Raissa Estrela Curado, Tais Sineiro Herig and Gleidson Silva Teixeira annotation2034977 1 primer_binding range2034977 1 1 19 annotation2040986 1 TATA-Like range2040986 1 862 873 annotation2034979 1 primer_binding range2034979 1 976 998 annotation2034978 1 JEN1 Promoter range2034978 1 1 998 BBa_E2030 1 BBa_E2030 enhanced version of EYFP, yeast-optimized 2005-01-24T12:00:00Z 2015-08-31T04:07:26Z nagai T. et al. (2002) Nat Biot (20):87-90 Released HQ 2013 Venus YFP, yeast codon optimized. EX515/EM528 false false _8_ 0 230 7 In stock false Contains N- and C-terminal linker sequences (with homology to biobrick parts BBa_E2060 (mCherry), BBa_E2050 (mOrange), and BBa_E2020 (Cerulean CFP) to facilitate color swapping in yeast. Adds N-"MATSG" and "GSGTA"-C. to published amino acid sequence. Inserts a "V" after normal start M. Double TAATAA stop codon. Missing EcoRI, HindIII, NotI, NdeI, XhoI, RsrII, BamHI, NcoI, BglI, SpeI, XbaI, and PstI. Except for 5' and 3' ends no significant sequence identity runs with Cerulean CFP (BBa_E2020) or to GFP S65T as found in O'Shea deletion strain collection (originally from plasmid pFA6-GFP(S65T)-His3MX6). true ryu annotation1431979 1 MATSG linker range1431979 1 1 15 annotation1431978 1 Venus YFP range1431978 1 1 747 annotation2214039 1 Help:Barcodes range2214039 1 754 778 annotation1431980 1 GSGTA linker range1431980 1 733 747 BBa_E2030_sequence 1 atggcaactagcggcatggttagtaaaggagaagaacttttcactggagttgtcccaattttagttgaactagatggcgacgtgaacggtcataagttcagtgtctccggcgaaggtgagggtgatgcaacgtacggtaagttaactttgaagttaatatgtacaaccggcaagctgcctgttccctggcctaccctggtgacaacgttaggttatgggttgatgtgctttgctagatacccagatcacatgaaaaggcatgacttctttaaatctgcaatgccagaaggttacgtccaagaacgtactattttctttaaagatgacggtaattataaaactagggctgaagttaaattcgaaggtgacacacttgtaaatcgaatagagttaaaggggattgatttcaaagaggatggtaatattctaggccataaacttgaatataactataattcacacaacgtttacattaccgccgacaagcagaagaatggaatcaaagccaattttaagattagacacaatattgaggatggtggagtacagcttgctgatcattaccaacaaaataccccgatcggtgatggaccagttttgctacccgataaccattatctgtcctatcaaagcaaattgtcaaaagatcctaacgaaaaaagagaccacatggtactcttggaatttgtaacagctgctgggattacacatggcatggatgaactatacaaaggttctggtaccgcataataaccctgatagtgctagtgtagatccc BBa_K284002_sequence 1 tgattggcaaacctgttgtcccgggaaacgaacaccacatatattatccggtgaagcgtggagtgaagcgtttcgcagtggcttttccaccacccccacgctccaacaacctacactatacgtgccgaaacggctaaatattagccgttctccacacactgcccgcagaaccctggaaggaaaagaaaagattttccctcctcgaagcgtggaacaacaatctctcttctcttctctccagtacttctgaccatcaaacaactcgataaaccacacatcctgcgattctcggagtctggggtgtgtttcctcctctggagcaacggacttgcctaattacagcgaatttccggagataggataatggcaatacccgtttgcttctaccacatgctgcatccgccaaccttacgaagggttcttcatgcccttttgcctcctctatgcgacttttaagctacttttagatatggggcatttgcttattgtccctgcgaattggcgactggataggcggatatatccaaattccgtaactgagatgatgatactctctcaaacacttaccttaaacgtcatgcttctgtgccggttactttggctattccatctggtttctttccttctttccttccttcctgaaacgctctccagttcttctctgttgagtgacgaggggcgagtattttccagtgttccggttatccaggtagtggttttgatttagttattgtgatgatctgtgaaacttttttttttttttttcactaacctcatctccggtacctttttactatctctcactaaaataactctaattcagtatgagcccgctggatgtaccacacctgcgtttcttgatatataaaagtaactggtttggcgtctttttttcgacaatcaacgttcttccaatcttgttgtggttgtgtcttattattattgtaatgtatgtcttgtatgatctggagtaaccaaaaccaaaacagatttcaacc BBa_K284020_sequence 1 tgattggcaaacctgttgtcccgggaaacgaacaccacatatattatccggtgaagcgtggagtgaagcgtttcgcagtggcttttccaccacccccacgctccaacaacctacactatacgtgccgaaacggctaaatattagccgttctccacacactgcccgcagaaccctggaaggaaaagaaaagattttccctcctcgaagcgtggaacaacaatctctcttctcttctctccagtacttctgaccatcaaacaactcgataaaccacacatcctgcgattctcggagtctggggtgtgtttcctcctctggagcaacggacttgcctaattacagcgaatttccggagataggataatggcaatacccgtttgcttctaccacatgctgcatccgccaaccttacgaagggttcttcatgcccttttgcctcctctatgcgacttttaagctacttttagatatggggcatttgcttattgtccctgcgaattggcgactggataggcggatatatccaaattccgtaactgagatgatgatactctctcaaacacttaccttaaacgtcatgcttctgtgccggttactttggctattccatctggtttctttccttctttccttccttcctgaaacgctctccagttcttctctgttgagtgacgaggggcgagtattttccagtgttccggttatccaggtagtggttttgatttagttattgtgatgatctgtgaaacttttttttttttttttcactaacctcatctccggtacctttttactatctctcactaaaataactctaattcagtatgagcccgctggatgtaccacacctgcgtttcttgatatataaaagtaactggtttggcgtctttttttcgacaatcaacgttcttccaatcttgttgtggttgtgtcttattattattgtaatgtatgtcttgtatgatctggagtaaccaaaaccaaaacagatttcaacctactagatggcaactagcggcatggttagtaaaggagaagaacttttcactggagttgtcccaattttagttgaactagatggcgacgtgaacggtcataagttcagtgtctccggcgaaggtgagggtgatgcaacgtacggtaagttaactttgaagttaatatgtacaaccggcaagctgcctgttccctggcctaccctggtgacaacgttaggttatgggttgatgtgctttgctagatacccagatcacatgaaaaggcatgacttctttaaatctgcaatgccagaaggttacgtccaagaacgtactattttctttaaagatgacggtaattataaaactagggctgaagttaaattcgaaggtgacacacttgtaaatcgaatagagttaaaggggattgatttcaaagaggatggtaatattctaggccataaacttgaatataactataattcacacaacgtttacattaccgccgacaagcagaagaatggaatcaaagccaattttaagattagacacaatattgaggatggtggagtacagcttgctgatcattaccaacaaaataccccgatcggtgatggaccagttttgctacccgataaccattatctgtcctatcaaagcaaattgtcaaaagatcctaacgaaaaaagagaccacatggtactcttggaatttgtaacagctgctgggattacacatggcatggatgaactatacaaaggttctggtaccgcataataaccctgatagtgctagtgtagatccc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z