LOCUS       BBa_K284021             1247 bp    DNA     linear       28-Jun-2024
DEFINITION  EYFP regulated by lactate reponsive  promoter (DLD)
ACCESSION   BBa_K284021
VERSION     BBa_K284021.1  
FEATURES             Location/Qualifiers
     promoter        1..463
                     /label=DLD Promoter
     primer_bind     1..22
                     /label=primer_binding
     misc_feature    300..330
                     /label=TATA-Like
     misc_feature    355..372
                     /label=TATA-Like
     primer_bind     442..463
                     /label=primer_binding
     promoter        1..463
                     /label=BBa_K284003
     CDS             470..1216
                     /label=Venus YFP
     misc_feature    470..484
                     /label=MATSG linker
     misc_feature    1202..1216
                     /label=GSGTA linker
     misc_feature    1223..1247
                     /label=Help:Barcodes
     misc_feature    470..1247
                     /label=BBa_E2030
ORIGIN
        1 ggtcgctctt tctctctctc atctaccgaa tctgagagct gctcttgact ctccactggc
       61 tctcagtgct gcctcaaacc agtacaacaa gatccgatcc acgctacatc ccacgctacg
      121 aagtgaaaaa tcatcatgga aatccggcaa caaagaaaag ccagagaaga ccagaggaga
      181 gcaagccgga tatgtatcaa aacaagggtg gatgaaccaa gtcgtggagt cgacggtata
      241 tgattgttcc tattccgcgt ttgaactgtt tttttttttc tcagctatga acttgtactt
      301 atatatatat atatatatat atgtataatc gaacgccgtc tgtattccgc atatcaatat
      361 actatataat aaggtaacaa cttggtagag aaacttggta acttagcagc tgttttgtgt
      421 acttgttatc gcaaagtgca agaaagacca gaaacgcaac tcatactaga tggcaactag
      481 cggcatggtt agtaaaggag aagaactttt cactggagtt gtcccaattt tagttgaact
      541 agatggcgac gtgaacggtc ataagttcag tgtctccggc gaaggtgagg gtgatgcaac
      601 gtacggtaag ttaactttga agttaatatg tacaaccggc aagctgcctg ttccctggcc
      661 taccctggtg acaacgttag gttatgggtt gatgtgcttt gctagatacc cagatcacat
      721 gaaaaggcat gacttcttta aatctgcaat gccagaaggt tacgtccaag aacgtactat
      781 tttctttaaa gatgacggta attataaaac tagggctgaa gttaaattcg aaggtgacac
      841 acttgtaaat cgaatagagt taaaggggat tgatttcaaa gaggatggta atattctagg
      901 ccataaactt gaatataact ataattcaca caacgtttac attaccgccg acaagcagaa
      961 gaatggaatc aaagccaatt ttaagattag acacaatatt gaggatggtg gagtacagct
     1021 tgctgatcat taccaacaaa ataccccgat cggtgatgga ccagttttgc tacccgataa
     1081 ccattatctg tcctatcaaa gcaaattgtc aaaagatcct aacgaaaaaa gagaccacat
     1141 ggtactcttg gaatttgtaa cagctgctgg gattacacat ggcatggatg aactatacaa
     1201 aggttctggt accgcataat aaccctgata gtgctagtgt agatccc
//