BBa_K284003
1
BBa_K284003
Partial DLD Promoter from Kluyveromyces lactis
2009-09-25T11:00:00Z
2015-05-08T01:11:48Z
.
.
false
false
_386_
0
4968
9
It's complicated
true
.
false
Tais Sineiro Herig, Raissa Estrela Curado and Gleidson Silva Teixeira
annotation2034976
1
primer_binding
range2034976
1
442
463
annotation2034972
1
DLD Promoter
range2034972
1
1
463
annotation2034973
1
primer_binding
range2034973
1
1
22
annotation2034975
1
TATA-Like
range2034975
1
355
372
annotation2034974
1
TATA-Like
range2034974
1
300
330
BBa_K284021
1
BBa_K284021
EYFP regulated by lactate reponsive promoter (DLD)
2009-10-11T11:00:00Z
2015-05-08T01:11:48Z
.
.
false
false
_386_
0
4968
9
Not in stock
false
.
false
Gleidson Silva Teixeira
component2244311
1
BBa_K284003
component2244316
1
BBa_E2030
annotation2244311
1
BBa_K284003
range2244311
1
1
463
annotation2244316
1
BBa_E2030
range2244316
1
470
1247
BBa_E2030
1
BBa_E2030
enhanced version of EYFP, yeast-optimized
2005-01-24T12:00:00Z
2015-08-31T04:07:26Z
nagai T. et al. (2002) Nat Biot (20):87-90
Released HQ 2013
Venus YFP, yeast codon optimized. EX515/EM528
false
false
_8_
0
230
7
In stock
false
Contains N- and C-terminal linker sequences (with homology to biobrick parts BBa_E2060 (mCherry), BBa_E2050 (mOrange), and BBa_E2020 (Cerulean CFP) to facilitate color swapping in yeast. Adds N-"MATSG" and "GSGTA"-C. to published amino acid sequence. Inserts a "V" after normal start M. Double TAATAA stop codon. Missing EcoRI, HindIII, NotI, NdeI, XhoI, RsrII, BamHI, NcoI, BglI, SpeI, XbaI, and PstI. Except for 5' and 3' ends no significant sequence identity runs with Cerulean CFP (BBa_E2020) or to GFP S65T as found in O'Shea deletion strain collection (originally from plasmid pFA6-GFP(S65T)-His3MX6).
true
ryu
annotation1431979
1
MATSG linker
range1431979
1
1
15
annotation1431978
1
Venus YFP
range1431978
1
1
747
annotation2214039
1
Help:Barcodes
range2214039
1
754
778
annotation1431980
1
GSGTA linker
range1431980
1
733
747
BBa_K284003_sequence
1
ggtcgctctttctctctctcatctaccgaatctgagagctgctcttgactctccactggctctcagtgctgcctcaaaccagtacaacaagatccgatccacgctacatcccacgctacgaagtgaaaaatcatcatggaaatccggcaacaaagaaaagccagagaagaccagaggagagcaagccggatatgtatcaaaacaagggtggatgaaccaagtcgtggagtcgacggtatatgattgttcctattccgcgtttgaactgtttttttttttctcagctatgaacttgtacttatatatatatatatatatatatgtataatcgaacgccgtctgtattccgcatatcaatatactatataataaggtaacaacttggtagagaaacttggtaacttagcagctgttttgtgtacttgttatcgcaaagtgcaagaaagaccagaaacgcaactca
BBa_E2030_sequence
1
atggcaactagcggcatggttagtaaaggagaagaacttttcactggagttgtcccaattttagttgaactagatggcgacgtgaacggtcataagttcagtgtctccggcgaaggtgagggtgatgcaacgtacggtaagttaactttgaagttaatatgtacaaccggcaagctgcctgttccctggcctaccctggtgacaacgttaggttatgggttgatgtgctttgctagatacccagatcacatgaaaaggcatgacttctttaaatctgcaatgccagaaggttacgtccaagaacgtactattttctttaaagatgacggtaattataaaactagggctgaagttaaattcgaaggtgacacacttgtaaatcgaatagagttaaaggggattgatttcaaagaggatggtaatattctaggccataaacttgaatataactataattcacacaacgtttacattaccgccgacaagcagaagaatggaatcaaagccaattttaagattagacacaatattgaggatggtggagtacagcttgctgatcattaccaacaaaataccccgatcggtgatggaccagttttgctacccgataaccattatctgtcctatcaaagcaaattgtcaaaagatcctaacgaaaaaagagaccacatggtactcttggaatttgtaacagctgctgggattacacatggcatggatgaactatacaaaggttctggtaccgcataataaccctgatagtgctagtgtagatccc
BBa_K284021_sequence
1
ggtcgctctttctctctctcatctaccgaatctgagagctgctcttgactctccactggctctcagtgctgcctcaaaccagtacaacaagatccgatccacgctacatcccacgctacgaagtgaaaaatcatcatggaaatccggcaacaaagaaaagccagagaagaccagaggagagcaagccggatatgtatcaaaacaagggtggatgaaccaagtcgtggagtcgacggtatatgattgttcctattccgcgtttgaactgtttttttttttctcagctatgaacttgtacttatatatatatatatatatatatgtataatcgaacgccgtctgtattccgcatatcaatatactatataataaggtaacaacttggtagagaaacttggtaacttagcagctgttttgtgtacttgttatcgcaaagtgcaagaaagaccagaaacgcaactcatactagatggcaactagcggcatggttagtaaaggagaagaacttttcactggagttgtcccaattttagttgaactagatggcgacgtgaacggtcataagttcagtgtctccggcgaaggtgagggtgatgcaacgtacggtaagttaactttgaagttaatatgtacaaccggcaagctgcctgttccctggcctaccctggtgacaacgttaggttatgggttgatgtgctttgctagatacccagatcacatgaaaaggcatgacttctttaaatctgcaatgccagaaggttacgtccaagaacgtactattttctttaaagatgacggtaattataaaactagggctgaagttaaattcgaaggtgacacacttgtaaatcgaatagagttaaaggggattgatttcaaagaggatggtaatattctaggccataaacttgaatataactataattcacacaacgtttacattaccgccgacaagcagaagaatggaatcaaagccaattttaagattagacacaatattgaggatggtggagtacagcttgctgatcattaccaacaaaataccccgatcggtgatggaccagttttgctacccgataaccattatctgtcctatcaaagcaaattgtcaaaagatcctaacgaaaaaagagaccacatggtactcttggaatttgtaacagctgctgggattacacatggcatggatgaactatacaaaggttctggtaccgcataataaccctgatagtgctagtgtagatccc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z