BBa_K284027
1
BBa_K284027
QseA promoter - activated by AI-2
2009-10-18T11:00:00Z
2015-05-08T01:11:48Z
Genomic sequence of Escherichia coli str. K-12 substr. MG1655.
QseA promoter indirectly activated by AI-2.
false
false
_386_
0
4973
9
Not in stock
false
The genomic sequence was amplified using the primers below:
pQseA-F 5??? ttctagagg GCCTGCGTGAGTTTGGTTA 3???
pQseA-R 5??? ctactagta TACTTTGGCAAACACCGACA 3???
false
Ane Fernanda Beraldi Zeidler
annotation2054609
1
QseA
range2054609
1
1
243
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1686
1
T7 TE
range1686
1
8
27
annotation1687
1
stop
range1687
1
34
34
annotation1690
1
polya
range1690
1
28
41
annotation7020
1
BBa_B0012
range7020
1
1
41
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_B0011
1
BBa_B0011
LuxICDABEG (+/-)
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>.
Released HQ 2013
Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.</p>
false
false
_1_
0
24
7
In stock
false
<P> <P>In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.<P>
true
Reshma Shetty
annotation7019
1
BBa_B0011
range7019
1
1
46
annotation1683
1
stem_loop
range1683
1
13
35
BBa_K112806
1
BBa_K112806
[T4 endolysin]
2008-10-31T12:00:00Z
2015-05-08T01:09:19Z
Enterobacteria phage T4
http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=29366675&from=66503&to=66997&view=gbwithparts
Released HQ 2013
The lysozyme from enterobacteria phage T4 degrades peptidoglycan layer.
false
true
_224_
0
3025
171
In stock
false
High expression of lysozyme only is toxic to bacteria.
false
Jin Huh
annotation1999018
1
T4 Endolysin
range1999018
1
17
511
BBa_K284035
1
BBa_K284035
T4 endolysin under the control of QseA promoter
2009-10-20T11:00:00Z
2015-05-08T01:11:48Z
This device is a junction of previously designed parts: BBa_K284027 - UNICAMP-BRAZIL 2008 - BBa_B0014 - BBa_K112806 - Designed by Jin Huh Group: iGEM08_UC_Berkeley and Bba_B0014
This construction is a device composed of: BBa_K284027 - BBa_B0034 - BBa_K112806 - Bba_B0014. This device contain the T4 endolysin, a lysozyme from enterobacteria phage, that degrades peptidoglycan layer, under the control of the QseA promoter. This promoter is activated by the auto-inducer AI-2.
false
false
_386_
0
4973
9
Not in stock
false
The QseA promotor is indirectly activated by AI-2.
false
Ane Fernanda Beraldi Zeidler
component2059075
1
BBa_B0011
component2059066
1
BBa_K284027
component2059070
1
BBa_K112806
component2059071
1
BBa_B0012
component2059068
1
BBa_B0034
annotation2059068
1
BBa_B0034
range2059068
1
252
263
annotation2059070
1
BBa_K112806
range2059070
1
272
785
annotation2059071
1
BBa_B0012
range2059071
1
794
834
annotation2059075
1
BBa_B0011
range2059075
1
843
888
annotation2059066
1
BBa_K284027
range2059066
1
1
243
BBa_K284035_sequence
1
gcctgcgtgagtttggttaatgttgaccttggttgtaaagagagagcaggcgttattattttcagcatctgtcgccgcagagaagggcatggaaagccgggcgagagcaacattgctgtagattgatatttaatatattagcgtaactgttatgctgttatctatattatgtgatctaaatcacttttaagtcagagtgaataaatggaacgactaaaacgcatgtcggtgtttgccaaagtatactagagaaagaggagaaatactagagatacttaggaggtattatgaatatatttgaaatgttacgtatagatgaaggtcttagacttaaaatctataaagacacagaaggctattacactattggcatcggtcatttgcttacaaaaagtccatcacttaatgctgctaaatctgaattagataaagctattgggcgtaattgcaatggtgtaattacaaaagatgaggctgaaaaactctttaatcaggatgttgatgctgctgttcgcggaatcctgagaaatgctaaattaaaaccggtttatgattctcttgatgcggttcgtcgctgtgcattgattaatatggttttccaaatgggagaaaccggtgtggcaggatttactaactctttacgtatgcttcaacaaaaacgctgggatgaagcagcagttaacttagctaaaagtagatggtataatcaaacacctaatcgcgcaaaacgagtcattacaacgtttagaactggcacttgggacgcgtataaaaatctataaagctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K284027_sequence
1
gcctgcgtgagtttggttaatgttgaccttggttgtaaagagagagcaggcgttattattttcagcatctgtcgccgcagagaagggcatggaaagccgggcgagagcaacattgctgtagattgatatttaatatattagcgtaactgttatgctgttatctatattatgtgatctaaatcacttttaagtcagagtgaataaatggaacgactaaaacgcatgtcggtgtttgccaaagta
BBa_B0011_sequence
1
agagaatataaaaagccagattattaatccggcttttttattattt
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K112806_sequence
1
atacttaggaggtattatgaatatatttgaaatgttacgtatagatgaaggtcttagacttaaaatctataaagacacagaaggctattacactattggcatcggtcatttgcttacaaaaagtccatcacttaatgctgctaaatctgaattagataaagctattgggcgtaattgcaatggtgtaattacaaaagatgaggctgaaaaactctttaatcaggatgttgatgctgctgttcgcggaatcctgagaaatgctaaattaaaaccggtttatgattctcttgatgcggttcgtcgctgtgcattgattaatatggttttccaaatgggagaaaccggtgtggcaggatttactaactctttacgtatgcttcaacaaaaacgctgggatgaagcagcagttaacttagctaaaagtagatggtataatcaaacacctaatcgcgcaaaacgagtcattacaacgtttagaactggcacttgggacgcgtataaaaatctataaagc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z