BBa_K284033
1
BBa_K284033
finP misc_RNA from Plasmid F
2009-10-15T11:00:00Z
2015-05-08T01:11:48Z
E. coli F plasmid (conjugative)
Antisense RNA of tra operon; binds traJ mRNA leader and blocks translation; requires the RNA-binding protein FinO to achieve duplex formation.
false
false
_386_
0
4974
9
Not in stock
false
Finp was amplified from E. coli F plasmid (conjugative).
false
Gabriel Francisco Zaniboni, Marcelo Colika Bassalo, Victor Augusti Negri and Bruno Vaz de oliveira
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1690
1
polya
range1690
1
28
41
annotation1687
1
stop
range1687
1
34
34
annotation1686
1
T7 TE
range1686
1
8
27
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation7018
1
BBa_B0010
range7018
1
1
80
annotation4184
1
stem_loop
range4184
1
12
55
BBa_R0040
1
p(tetR)
TetR repressible promoter
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
Lutz, R., Bujard, H., <em>Nucleic Acids Research</em> (1997) 25, 1203-1210.
Released HQ 2013
Sequence for pTet inverting regulator driven by the TetR protein.</P>
false
true
_1_
0
24
7
In stock
false
<P> <P>BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.<P>
true
June Rhee, Connie Tao, Ty Thomson, Louis Waldman
annotation1986786
1
TetR 2
range1986786
1
26
44
annotation1986787
1
-10
range1986787
1
43
48
annotation1986783
1
TetR 1
range1986783
1
1
19
annotation1986784
1
BBa_R0040
range1986784
1
1
54
annotation1986785
1
-35
range1986785
1
20
25
BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation1701
1
RBS-1\Strong
range1701
1
1
15
annotation7025
1
BBa_B0030
range7025
1
1
15
annotation1702
1
RBS
range1702
1
8
12
BBa_K284060
1
BBa_K284060
FinP under constitutive promoter
2009-10-16T11:00:00Z
2015-05-08T01:11:48Z
Assemby using other biobricks
Antisense RNA of tra operon; binds traJ mRNA leader and blocks translation; requires the RNA-binding protein FinO to achieve duplex formation. In this device, FinP is under regulation of pTet.
false
false
_386_
0
4974
9
Not in stock
false
no consideration
false
Gabriel Francisco Zaniboni, Marcelo Colika Bassalo, Victor Augusti Negri and Bruno Vaz de oliveira
component2048809
1
BBa_R0040
component2048817
1
BBa_K284033
component2048818
1
BBa_B0010
component2048820
1
BBa_B0012
component2048815
1
BBa_B0030
annotation2048809
1
BBa_R0040
range2048809
1
1
54
annotation2048815
1
BBa_B0030
range2048815
1
63
77
annotation2048820
1
BBa_B0012
range2048820
1
295
335
annotation2048818
1
BBa_B0010
range2048818
1
207
286
annotation2048817
1
BBa_K284033
range2048817
1
86
198
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_R0040_sequence
1
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_K284060_sequence
1
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagattaaagaggagaaatactagagtgacgagcatgtttttgttgaatacgatccatcggatacataggaacctcctcacaaaggattctatggacagtcgatgcagggagttcacgtctccctgcatcggcgatttttactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K284033_sequence
1
tgacgagcatgtttttgttgaatacgatccatcggatacataggaacctcctcacaaaggattctatggacagtcgatgcagggagttcacgtctccctgcatcggcgatttt
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z