BBa_R0040
1
p(tetR)
TetR repressible promoter
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
Lutz, R., Bujard, H., <em>Nucleic Acids Research</em> (1997) 25, 1203-1210.
Released HQ 2013
Sequence for pTet inverting regulator driven by the TetR protein.</P>
false
true
_1_
0
24
7
In stock
false
<P> <P>BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.<P>
true
June Rhee, Connie Tao, Ty Thomson, Louis Waldman
annotation1986785
1
-35
range1986785
1
20
25
annotation1986783
1
TetR 1
range1986783
1
1
19
annotation1986784
1
BBa_R0040
range1986784
1
1
54
annotation1986787
1
-10
range1986787
1
43
48
annotation1986786
1
TetR 2
range1986786
1
26
44
BBa_K284080
1
BBa_K284080
Upstream construction flanking FSTK gene
2009-10-16T11:00:00Z
2015-05-08T01:11:48Z
Assembly from other biobricks
This divice blocks conjugation by acting on the TraJ Operon. It can be excised form the genome by a Cre recombinase, which recognizes the Lox66 site.
false
false
_386_
0
4974
9
Not in stock
false
no considerations
false
Gabriel Francisco Zaniboni, Victor Augusti Negri, Marcelo Colika Bassalo and Bruno Vaz de oliveira
component2048893
1
BBa_I718016
component2048860
1
BBa_R0040
component2048875
1
BBa_R0040
component2048887
1
BBa_B0010
component2048869
1
BBa_B0010
component2048886
1
BBa_K284032
component2048881
1
BBa_B0030
component2048866
1
BBa_B0030
component2048889
1
BBa_B0012
component2048868
1
BBa_K284033
component2048871
1
BBa_B0012
annotation2048889
1
BBa_B0012
range2048889
1
1084
1124
annotation2048887
1
BBa_B0010
range2048887
1
996
1075
annotation2048866
1
BBa_B0030
range2048866
1
63
77
annotation2048893
1
BBa_I718016
range2048893
1
1133
1166
annotation2048869
1
BBa_B0010
range2048869
1
207
286
annotation2048881
1
BBa_B0030
range2048881
1
406
420
annotation2048886
1
BBa_K284032
range2048886
1
427
987
annotation2048875
1
BBa_R0040
range2048875
1
344
397
annotation2048868
1
BBa_K284033
range2048868
1
86
198
annotation2048871
1
BBa_B0012
range2048871
1
295
335
annotation2048860
1
BBa_R0040
range2048860
1
1
54
BBa_K284033
1
BBa_K284033
finP misc_RNA from Plasmid F
2009-10-15T11:00:00Z
2015-05-08T01:11:48Z
E. coli F plasmid (conjugative)
Antisense RNA of tra operon; binds traJ mRNA leader and blocks translation; requires the RNA-binding protein FinO to achieve duplex formation.
false
false
_386_
0
4974
9
Not in stock
false
Finp was amplified from E. coli F plasmid (conjugative).
false
Gabriel Francisco Zaniboni, Marcelo Colika Bassalo, Victor Augusti Negri and Bruno Vaz de oliveira
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1686
1
T7 TE
range1686
1
8
27
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1687
1
stop
range1687
1
34
34
annotation1690
1
polya
range1690
1
28
41
BBa_K284032
1
BBa_K284032
finO conjugal transfer fertility inhibition protein FinO from Plasmid R100
2009-10-15T11:00:00Z
2015-05-08T01:11:48Z
E. coli R100 Plasmid (conjugative)
the basic protein FinO is part of the the two component FinOP system which is responsible for repressing bacterial conjugation; the FinOP system represses the transfer (tra) operon of the F-plasmid which encodes the proteins responsible for conjugative transfer of this plasmid from host to recipient Escherichia coli cells; antisense RNA, FinP is thought to interact with traJ mRNA to occlude its ribosome binding site, blocking traJ translation and thereby inhibiting transcription of the tra operon; FinO protects FinP against degradation by binding to FinP and sterically blocking the cellular endonuclease RNase E; FinO also also binds to the complementary stem-loop structures in traJ mRNA and promotes duplex formation between FinP and traJ RNA in vitro; this domain contains two independent RNA binding regions.
false
false
_386_
0
4974
9
Not in stock
false
FinO was amplified from E. coli R100 Plasmid (conjugative)
false
Marcelo Colika Bassalo, Gabriel Francisco Zaniboni, Victor Augusti Negri and Bruno Vaz de oliveira
annotation2046231
1
stop
range2046231
1
559
561
annotation2046230
1
start
range2046230
1
1
3
annotation2046232
1
cds
range2046232
1
1
561
BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation7025
1
BBa_B0030
range7025
1
1
15
annotation1702
1
RBS
range1702
1
8
12
annotation1701
1
RBS-1\Strong
range1701
1
1
15
BBa_I718016
1
lox66
lox66
2007-10-25T11:00:00Z
2015-08-31T04:07:52Z
This part was generated in the form of a forward & a reverse primer. After annealing these primers EcoRI & PstI compatible cohesive ends at the 5' & 3' ends of the dsDNA were generated.
Next, the dsDNA was subcloned in a pSB1A2 open plasmid (digested with EcoRI & PstI)
You can follow the construction process by following the links available in the Paris iGEM 2007 wiki:
http://parts.mit.edu/igem07/index.php/Paris
"freezer" section
plasmids table. A links sends you to the corresponding notebook date when the ligation reaction was performed
lox66 is a site specific recombination cassette. It belongs to the loxP family frequently used in genetics, particularily in mouse genetics.
lox site recombination is catalysed by a Site specific recombinase, Cre.
lox sequences are composed of an 8 bp Core sequence surrounded by two Arms.
The particularity of lox66 is that it has an altered sequence at the end of it's left arm compared to loxP. This sequence variation reduces affinity of the Cre recombinase for the arm.
As a consequence, after a recombination between a lox66 and a lox71 (altered right arm sequence), one of the two resulting generated lox sites has very low recombination potential as it inherited both mutated arms. Use of lox66 & lox71 sites is potentially interresting when the recombination reaction must be "irreversible".
false
false
_141_
0
1568
9
In stock
false
No modidification was made on the lox66 sequence
true
Eimad Shotar
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation7018
1
BBa_B0010
range7018
1
1
80
annotation4184
1
stem_loop
range4184
1
12
55
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_K284032_sequence
1
atgacagagcagaaacgaccggtactgacactgaaacggaaaacggaaggggaaacaccgacccggagccggaaaaccatcatcaatgtcaccacgccaccaaaatggaaggtgaaaaagcagaaactggcggagaaggctgcccgggaagcagagctgacagcaaaaaaagcgcaggccagacaggcgctgtccatttatctgaacctgccctcgctggatgaggccgtgaacaccctgaaaccctggtggccgggattatttgacggtgacacaccccgacttctggcctgcggtatccgggacgtgttactggaagacgtggcgcagcggaatatcccgctctcgcataaaaaactgcgcagggcgctgaaggccatcacccgttcagaaagctatctgtgtgccatgaaagccggtgcctgccggtatgacacggaagggtatgtgacggagcatatttctcaggaggaagaagtgtatgcggcagagcgtctggataaaatccgccgccagaaccggataaaggcagaacttcaggccgtgcttgatgaacaataa
BBa_R0040_sequence
1
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_K284080_sequence
1
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagattaaagaggagaaatactagagtgacgagcatgtttttgttgaatacgatccatcggatacataggaacctcctcacaaaggattctatggacagtcgatgcagggagttcacgtctccctgcatcggcgatttttactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagattaaagaggagaaatactagatgacagagcagaaacgaccggtactgacactgaaacggaaaacggaaggggaaacaccgacccggagccggaaaaccatcatcaatgtcaccacgccaccaaaatggaaggtgaaaaagcagaaactggcggagaaggctgcccgggaagcagagctgacagcaaaaaaagcgcaggccagacaggcgctgtccatttatctgaacctgccctcgctggatgaggccgtgaacaccctgaaaccctggtggccgggattatttgacggtgacacaccccgacttctggcctgcggtatccgggacgtgttactggaagacgtggcgcagcggaatatcccgctctcgcataaaaaactgcgcagggcgctgaaggccatcacccgttcagaaagctatctgtgtgccatgaaagccggtgcctgccggtatgacacggaagggtatgtgacggagcatatttctcaggaggaagaagtgtatgcggcagagcgtctggataaaatccgccgccagaaccggataaaggcagaacttcaggccgtgcttgatgaacaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagataacttggtatagcatacattatacgaacggta
BBa_I718016_sequence
1
ataacttggtatagcatacattatacgaacggta
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K284033_sequence
1
tgacgagcatgtttttgttgaatacgatccatcggatacataggaacctcctcacaaaggattctatggacagtcgatgcagggagttcacgtctccctgcatcggcgatttt
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z