BBa_K284080
type: ComponentDefinition
persistent identity: BBa K284080
display id: BBa_K284080
version: 1
was generated by: iGEM to SBOL conversion
description: Upstream construction flanking FSTK gene
created: 2009-10-16T11:00:00Z
modified: 2015-05-08T01:11:48Z
mutable provenance: Assembly from other biobricks
owned by: james
owned by: myers
mutable description: This divice blocks conjugation by acting on the TraJ Operon. It can be excised form the genome by a Cre recombinase, which recognizes the Lox66 site.
discontinued: false
dominant: false
experience: experience/None
group u list: _386_
m user id: 0
owner id: 4974
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: no considerations
star: false
creator: Gabriel Francisco Zaniboni, Victor Augusti Negri, Marcelo Colika Bassalo and Bruno Vaz de oliveira
type: DnaRegion
role: SO:0000804
role: partType/Device
component: BBa_I718016
component: BBa_K284032
component: BBa_B0030
component: BBa_B0012
component: BBa_B0010
component: BBa_K284033
component: BBa_R0040
sequence annotation: BBa_R0040
sequence annotation: BBa_B0030
sequence annotation: BBa_K284033
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_K284032
sequence annotation: BBa_I718016
sequence: https://synbiohub.org/public/igem/BBa_K284080_sequence/1

BBa_I718016
type: Component
persistent identity: component2048893
display id: component2048893
version: 1
top level: BBa_K284080
definition: lox66
access: public

BBa_K284032
type: Component
persistent identity: component2048886
display id: component2048886
version: 1
top level: BBa_K284080
definition: BBa_K284032
access: public

BBa_B0030
type: Component
persistent identity: component2048881
display id: component2048881
version: 1
top level: BBa_K284080
definition: BBa_B0030
access: public

BBa_B0012
type: Component
persistent identity: component2048889
display id: component2048889
version: 1
top level: BBa_K284080
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component2048887
display id: component2048887
version: 1
top level: BBa_K284080
definition: BBa_B0010
access: public

BBa_B0010
type: Component
persistent identity: component2048869
display id: component2048869
version: 1
top level: BBa_K284080
definition: BBa_B0010
access: public

BBa_K284033
type: Component
persistent identity: component2048868
display id: component2048868
version: 1
top level: BBa_K284080
definition: BBa_K284033
access: public

BBa_R0040
type: Component
persistent identity: component2048875
display id: component2048875
version: 1
top level: BBa_K284080
definition: p(tetR)
access: public

BBa_B0012
type: Component
persistent identity: component2048871
display id: component2048871
version: 1
top level: BBa_K284080
definition: BBa_B0012
access: public

BBa_B0030
type: Component
persistent identity: component2048866
display id: component2048866
version: 1
top level: BBa_K284080
definition: BBa_B0030
access: public

BBa_R0040
type: Component
persistent identity: component2048860
display id: component2048860
version: 1
top level: BBa_K284080
definition: p(tetR)
access: public

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2048860
display id: annotation2048860
version: 1
top level: BBa_K284080
location: https://synbiohub.org/public/igem/BBa_K284080/annotation2048860/range2048860/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K284080/annotation2048860/range2048860/1
type: Range
persistent identity: range2048860
display id: range2048860
version: 1
top level: BBa_K284080
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2048866
display id: annotation2048866
version: 1
top level: BBa_K284080
location: https://synbiohub.org/public/igem/BBa_K284080/annotation2048866/range2048866/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K284080/annotation2048866/range2048866/1
type: Range
persistent identity: range2048866
display id: range2048866
version: 1
top level: BBa_K284080
direction: direction/forward
start: 63
end: 77
orientation: inline

BBa_K284033
type: SequenceAnnotation
persistent identity: annotation2048868
display id: annotation2048868
version: 1
top level: BBa_K284080
location: https://synbiohub.org/public/igem/BBa_K284080/annotation2048868/range2048868/1
component: BBa_K284033

https://synbiohub.org/public/igem/BBa_K284080/annotation2048868/range2048868/1
type: Range
persistent identity: range2048868
display id: range2048868
version: 1
top level: BBa_K284080
direction: direction/reverse
start: 86
end: 198
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2048869
display id: annotation2048869
version: 1
top level: BBa_K284080
location: https://synbiohub.org/public/igem/BBa_K284080/annotation2048869/range2048869/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K284080/annotation2048869/range2048869/1
type: Range
persistent identity: range2048869
display id: range2048869
version: 1
top level: BBa_K284080
direction: direction/forward
start: 207
end: 286
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2048871
display id: annotation2048871
version: 1
top level: BBa_K284080
location: https://synbiohub.org/public/igem/BBa_K284080/annotation2048871/range2048871/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K284080/annotation2048871/range2048871/1
type: Range
persistent identity: range2048871
display id: range2048871
version: 1
top level: BBa_K284080
direction: direction/forward
start: 295
end: 335
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2048875
display id: annotation2048875
version: 1
top level: BBa_K284080
location: https://synbiohub.org/public/igem/BBa_K284080/annotation2048875/range2048875/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K284080/annotation2048875/range2048875/1
type: Range
persistent identity: range2048875
display id: range2048875
version: 1
top level: BBa_K284080
direction: direction/forward
start: 344
end: 397
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2048881
display id: annotation2048881
version: 1
top level: BBa_K284080
location: https://synbiohub.org/public/igem/BBa_K284080/annotation2048881/range2048881/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K284080/annotation2048881/range2048881/1
type: Range
persistent identity: range2048881
display id: range2048881
version: 1
top level: BBa_K284080
direction: direction/forward
start: 406
end: 420
orientation: inline

BBa_K284032
type: SequenceAnnotation
persistent identity: annotation2048886
display id: annotation2048886
version: 1
top level: BBa_K284080
location: https://synbiohub.org/public/igem/BBa_K284080/annotation2048886/range2048886/1
component: BBa_K284032

https://synbiohub.org/public/igem/BBa_K284080/annotation2048886/range2048886/1
type: Range
persistent identity: range2048886
display id: range2048886
version: 1
top level: BBa_K284080
direction: direction/reverse
start: 427
end: 987
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2048887
display id: annotation2048887
version: 1
top level: BBa_K284080
location: https://synbiohub.org/public/igem/BBa_K284080/annotation2048887/range2048887/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K284080/annotation2048887/range2048887/1
type: Range
persistent identity: range2048887
display id: range2048887
version: 1
top level: BBa_K284080
direction: direction/forward
start: 996
end: 1075
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2048889
display id: annotation2048889
version: 1
top level: BBa_K284080
location: https://synbiohub.org/public/igem/BBa_K284080/annotation2048889/range2048889/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K284080/annotation2048889/range2048889/1
type: Range
persistent identity: range2048889
display id: range2048889
version: 1
top level: BBa_K284080
direction: direction/forward
start: 1084
end: 1124
orientation: inline

BBa_I718016
type: SequenceAnnotation
persistent identity: annotation2048893
display id: annotation2048893
version: 1
top level: BBa_K284080
location: https://synbiohub.org/public/igem/BBa_K284080/annotation2048893/range2048893/1
component: BBa_I718016

https://synbiohub.org/public/igem/BBa_K284080/annotation2048893/range2048893/1
type: Range
persistent identity: range2048893
display id: range2048893
version: 1
top level: BBa_K284080
direction: direction/reverse
start: 1133
end: 1166
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.1 (strong) -- modified from R. Weiss
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-1\Strong
sequence annotation: BBa_B0030
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
top level: BBa_B0030
direction: direction/forward
start: 8
end: 12
orientation: inline

lox66
type: ComponentDefinition
persistent identity: BBa I718016
display id: BBa_I718016
version: 1
was generated by: iGEM to SBOL conversion
description: lox66
created: 2007-10-25T11:00:00Z
modified: 2015-08-31T04:07:52Z
mutable provenance: This part was generated in the form of a forward & a reverse primer. After annealing these primers EcoRI & PstI compatible cohesive ends at the 5' & 3' ends of the dsDNA were generated. Next, the dsDNA was subcloned in a pSB1A2 open plasmid (digested with EcoRI & PstI) You can follow the construction process by following the links available in the Paris iGEM 2007 wiki: http://parts.mit.edu/igem07/index.php/Paris "freezer" section plasmids table. A links sends you to the corresponding notebook date when the ligation reaction was performed
owned by: james
owned by: myers
mutable description: lox66 is a site specific recombination cassette. It belongs to the loxP family frequently used in genetics, particularily in mouse genetics. lox site recombination is catalysed by a Site specific recombinase, Cre. lox sequences are composed of an 8 bp Core sequence surrounded by two Arms. The particularity of lox66 is that it has an altered sequence at the end of it's left arm compared to loxP. This sequence variation reduces affinity of the Cre recombinase for the arm. As a consequence, after a recombination between a lox66 and a lox71 (altered right arm sequence), one of the two resulting generated lox sites has very low recombination potential as it inherited both mutated arms. Use of lox66 & lox71 sites is potentially interresting when the recombination reaction must be "irreversible".
discontinued: false
dominant: false
experience: experience/None
group u list: _141_
m user id: 0
owner id: 1568
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: No modidification was made on the lox66 sequence
star: true
creator: Eimad Shotar
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_I718016_sequence/1

BBa_K284032
type: ComponentDefinition
persistent identity: BBa K284032
display id: BBa_K284032
version: 1
was generated by: iGEM to SBOL conversion
description: finO conjugal transfer fertility inhibition protein FinO from Plasmid R100
created: 2009-10-15T11:00:00Z
modified: 2015-05-08T01:11:48Z
mutable provenance: E. coli R100 Plasmid (conjugative)
owned by: james
owned by: myers
mutable description: the basic protein FinO is part of the the two component FinOP system which is responsible for repressing bacterial conjugation; the FinOP system represses the transfer (tra) operon of the F-plasmid which encodes the proteins responsible for conjugative transfer of this plasmid from host to recipient Escherichia coli cells; antisense RNA, FinP is thought to interact with traJ mRNA to occlude its ribosome binding site, blocking traJ translation and thereby inhibiting transcription of the tra operon; FinO protects FinP against degradation by binding to FinP and sterically blocking the cellular endonuclease RNase E; FinO also also binds to the complementary stem-loop structures in traJ mRNA and promotes duplex formation between FinP and traJ RNA in vitro; this domain contains two independent RNA binding regions.
discontinued: false
dominant: false
experience: experience/None
group u list: _386_
m user id: 0
owner id: 4974
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: FinO was amplified from E. coli R100 Plasmid (conjugative)
star: false
creator: Marcelo Colika Bassalo, Gabriel Francisco Zaniboni, Victor Augusti Negri and Bruno Vaz de oliveira
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: start
sequence annotation: cds
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K284032_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2046230
display id: annotation2046230
version: 1
top level: BBa_K284032
location: https://synbiohub.org/public/igem/BBa_K284032/annotation2046230/range2046230/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K284032/annotation2046230/range2046230/1
type: Range
persistent identity: range2046230
display id: range2046230
version: 1
top level: BBa_K284032
direction: direction/forward
start: 1
end: 3
orientation: inline

cds
type: SequenceAnnotation
persistent identity: annotation2046232
display id: annotation2046232
version: 1
top level: BBa_K284032
location: https://synbiohub.org/public/igem/BBa_K284032/annotation2046232/range2046232/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K284032/annotation2046232/range2046232/1
type: Range
persistent identity: range2046232
display id: range2046232
version: 1
top level: BBa_K284032
direction: direction/forward
start: 1
end: 561
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2046231
display id: annotation2046231
version: 1
top level: BBa_K284032
location: https://synbiohub.org/public/igem/BBa_K284032/annotation2046231/range2046231/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K284032/annotation2046231/range2046231/1
type: Range
persistent identity: range2046231
display id: range2046231
version: 1
top level: BBa_K284032
direction: direction/forward
start: 559
end: 561
orientation: inline

BBa_K284033
type: ComponentDefinition
persistent identity: BBa K284033
display id: BBa_K284033
version: 1
was generated by: iGEM to SBOL conversion
description: finP misc_RNA from Plasmid F
created: 2009-10-15T11:00:00Z
modified: 2015-05-08T01:11:48Z
mutable provenance: E. coli F plasmid (conjugative)
owned by: james
owned by: myers
mutable description: Antisense RNA of tra operon; binds traJ mRNA leader and blocks translation; requires the RNA-binding protein FinO to achieve duplex formation.
discontinued: false
dominant: false
experience: experience/None
group u list: _386_
m user id: 0
owner id: 4974
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Finp was amplified from E. coli F plasmid (conjugative).
star: false
creator: Gabriel Francisco Zaniboni, Marcelo Colika Bassalo, Victor Augusti Negri and Bruno Vaz de oliveira
type: DnaRegion
role: partType/RNA
role: SO:0000834
sequence: https://synbiohub.org/public/igem/BBa_K284033_sequence/1

https://synbiohub.org/public/igem/BBa_K284080_sequence/1
type: Sequence
persistent identity: BBa K284080 sequence
display id: BBa_K284080_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagattaaagaggagaaatactagagtgacgagcatgtttttgttgaatacgatccatcggatacataggaacctcctcacaaaggattctatggacagtcgatgcagggagttcacgtctccctgcatcggcgatttttactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagattaaagaggagaaatactagatgacagagcagaaacgaccggtactgacactgaaacggaaaacggaaggggaaacaccgacccggagccggaaaaccatcatcaatgtcaccacgccaccaaaatggaaggtgaaaaagcagaaactggcggagaaggctgcccgggaagcagagctgacagcaaaaaaagcgcaggccagacaggcgctgtccatttatctgaacctgccctcgctggatgaggccgtgaacaccctgaaaccctggtggccgggattatttgacggtgacacaccccgacttctggcctgcggtatccgggacgtgttactggaagacgtggcgcagcggaatatcccgctctcgcataaaaaactgcgcagggcgctgaaggccatcacccgttcagaaagctatctgtgtgccatgaaagccggtgcctgccggtatgacacggaagggtatgtgacggagcatatttctcaggaggaagaagtgtatgcggcagagcgtctggataaaatccgccgccagaaccggataaaggcagaacttcaggccgtgcttgatgaacaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagataacttggtatagcatacattatacgaacggta
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0030_sequence/1
type: Sequence
persistent identity: BBa B0030 sequence
display id: BBa_B0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I718016_sequence/1
type: Sequence
persistent identity: BBa I718016 sequence
display id: BBa_I718016_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ataacttggtatagcatacattatacgaacggta
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K284032_sequence/1
type: Sequence
persistent identity: BBa K284032 sequence
display id: BBa_K284032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgacagagcagaaacgaccggtactgacactgaaacggaaaacggaaggggaaacaccgacccggagccggaaaaccatcatcaatgtcaccacgccaccaaaatggaaggtgaaaaagcagaaactggcggagaaggctgcccgggaagcagagctgacagcaaaaaaagcgcaggccagacaggcgctgtccatttatctgaacctgccctcgctggatgaggccgtgaacaccctgaaaccctggtggccgggattatttgacggtgacacaccccgacttctggcctgcggtatccgggacgtgttactggaagacgtggcgcagcggaatatcccgctctcgcataaaaaactgcgcagggcgctgaaggccatcacccgttcagaaagctatctgtgtgccatgaaagccggtgcctgccggtatgacacggaagggtatgtgacggagcatatttctcaggaggaagaagtgtatgcggcagagcgtctggataaaatccgccgccagaaccggataaaggcagaacttcaggccgtgcttgatgaacaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K284033_sequence/1
type: Sequence
persistent identity: BBa K284033 sequence
display id: BBa_K284033_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgacgagcatgtttttgttgaatacgatccatcggatacataggaacctcctcacaaaggattctatggacagtcgatgcagggagttcacgtctccctgcatcggcgatttt
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z