BBa_K284150 1 BBa_K284150 Alpha Hemolysin Secretion System 2009-10-19T11:00:00Z 2015-05-08T01:11:48Z This part come from plasmidial DNA. This biobrick contains part of the Alpha hemolysin operon with 252bp of hlyA and the complete sequencen of hlyD and hlyB. hlyA works as a signal peptide for extracellular transportation to proteins fused in the prefix, the target protein mustn't have the stop codon. HlyB and hlyD assemble to create the transport channel that recognize the hlyA signal peptide. false false _386_ 0 5132 9 Not in stock false For the primers design we chose the hemolysin from large plasmids of Escherichia coli instead of genomic hemolysin because we have a sorotype with this plasmid. false Marcos Henrique de Moraes BBa_K284150_sequence 1 gataaagatggtcgagagataaaagttgataaaatacctcataataataatgaacggtcaggttatataaaagccagtaatatagcatctgaaaaaaacatggttaatatcaccagtgttgccaatgatattaataagattatttcttcagtttcagggttcgattcaggtgatgaacgattagcatctttatataatttatccttacatcaaaacaacacacactcaacaactttaacgacaactgtctgagactaaaagaggaggtgatggccattccaggaggaatggtcatcgttatatgatgagtaaatgtagttctcataatagtctgtatgcactgatattgcttgcacaatatcataatataactgtcaatgctgaaactataaggcatcagtataatacccacacacaagattttggggtgactgaatggttactggcagcgaaatccattggcttaaaagcaaaatatgtagaaaaacatttttccagattgtcaataatttctttacctgcgttgatatggcgggatgacggtaagcattatatattgtctcgtgttactaaagattcatcacgctatcttgtttatgatccagaacaacatcagtcactaacttttagtcgggatgagtttgaaaaactgtatcagggaaaagtcattctggttacgtcaagagcaacagtagtcggagagttagctaaatttgatttttcttggtttatcccctctgttgtgaaatacaggaggattttacttgaggtgttaactgtttctgcttttattcagtttcttgcgttaataacacctcttttttttcaggttgtaatggataaggttttagttcaccgggggttttcaacgttaaatattatcacaatagcatttattatagtgatactttttgaagtgatattaaccggagccagaacttatattttctctcatactacaagtcgtattgacgtcgaactgagcgctaagttattcagacatttgcttgcattgcctgtttcatattttgaaaatcgcagggtcggagagaccgttgccagagtaagggaactggagcaaattcgtaattttttaaccggacaagcgttgacatcagttcttgatctatttttttctgtaatatttttttgtgtcatgtggtattacagccctcaattaacactggttatattattgtcactaccttgttatgttatatggtcattgtttatatcacccttattacgtcgacgtcttgatgataagtttctcaggaatgcagaaaatcaagcttttcttgtcgaaacggtaacagcaataaatacaattaaatccatggcagtatcaccacagatgattgctacatgggataaacaactggccgggtatgttgcttccagtttcagggtaaatctggttgcaatgacagggcagcaggggatacagctgatacagaaaagtgtaatggtaattagtctatggatgggagcacatcttgtcatatcgggagagataagtatcgggcagttaatcgcgtttaatatgcttgcaggtcaggtcattgctcctgttatcagactggctcatctttggcaggattttcagcaggttggtatttctgttgagcgccttggtgatgtattaaatacaccggtagagaaaaagtcaggcagaaatatactgccggaaattcagggggatatcgaatttaaaaatgtcagatttcggtattcttctgacggtaatgttattttgaataatattaatttatacatatcaaagggggatgttatcggtatagttggccgttctggttcaggaaaaagtacattaacaaaactgcttcagcgcttttatataccagagaccggacagattttaattgatgggcatgatttatcacttgcagatccagaatggttacgacgccagattggtgttgtattgcaggaaaatatactattaaatcgtagtattatcgataatattacattagcttctccggctgtatctatggaacaggctattgaggcagccagacttgcaggtgcccatgattttattacagaactaaaagaagggtacaatactattgttggagaacagggcgttggtctttcaggagggcagcgccaacggatcgcaatagcccgggctcttgttacaaatcctcgaattcttatttttgatgaagcaaccagtgctcttgattatgagtcggaaaatataataatgaaaaatatgtcaagaatatgtaagaacagaaccgtaattattattgcacacaggttgtcaactgtaaaaaatgcaaacagaataattgttatggataatggctttatttctgaagatggcacacataaagagcttatctccaaaaaagacagtttatatgcatatttatatcagttgcaggcataacgtatgcgcttttacatgaaaggtttatgggatttggtttgccgttataagacggttttttctgatgtctggaaaattcgtcacacacttgatgctcctgtgagagaaaaagatgagtatgcatttcttcctgctcaccttgaactcattgaaacaccagtatccagacgttctcattttgttgtatggagtattttattatttgtaattatatctcttctcttatctgttcttgggaaagttgaagtggtttcagtagcaaatggtaagtttactcatagtggaagaagcaaagaaataaaaccgatagagaatgcgattgtagaaaaaataatggtaaaagatggctcttttgtaaaaaaaaatgatccattagttgaattgacggtgcctggtgttgaatctgatattttaaaatcagaagcatctttgttgtatgaaaaaacagaacaatatcgctacgcaattctttctgaatcaatacaacggaatgagcttcctgaaataaggataacagattttcctggcggagaagataacgcgggaggtgaacattttcagagggttagctcattaataaaagagcaattcatgacatggcagaacagaaaaaatcaaaagcagctcacattaaataaaaaaatagttgaacgggatgcagcgcttgctcgtgttagcctgtatgagcatcaggtatcacaggaaggaagaaaactcaatgattttaagtatttgttgaataaaaaagctgtttctcaacattcagttatggagcaggaaaatagctatattcaggcaaaaaatgaacatgcagtctggcttgcacaggtttctcaacttgaaaaagaaatagaacttgtgcgggaagaactggcactggagacgaatatcttcagaagtgaaattatcgagaagcacagaaaatcaacagataacattgtgttgctggagcatgagcttgaaaaaaacaggcagagaaaagcatcatcttttattaaagctcctgtgagtggtactgttcaggagttaaatatacatacagaaggtggtgttgtcacaacagcagaaacgctgatgattattgtccctgataatgatattctcgaagtaacagcatctgtacttaacaaggatatcggttttatacaacctggacaggaggtcgttattaaagtagatgcatatccatatacacgtcatggttaccttacagggaaagtaaaaaatataactgcagattctgtttctgttccggatacagggcttgtatttaacgtgattatatctgttgatcggaatgatatacagggagaaagaaaaaaaatccctgttacagctggaatgactgttatggcagaaataaaaaccggggtgcgtagtgttatcagctatcttcttagtccattaaaggaaactattaatgaaagtttacgtgaacgttag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z