LOCUS BBa_K287006 4218 bp DNA linear 16-Jun-2024 DEFINITION Double Channel with Cyanine reporter ACCESSION BBa_K287006 VERSION BBa_K287006.1 FEATURES Location/Qualifiers CDS 1..1333 /label=BBa_K287003 CDS 1342..3270 /label=BBa_K287004 misc_feature 3279..3297 /label=TetR 1 misc_feature 3279..3332 /label=BBa_R0040 promoter 3298..3303 /label=-35 misc_feature 3304..3322 /label=TetR 2 promoter 3321..3326 /label=-10 promoter 3279..3332 /label=BBa_R0040 misc_feature 3345..3348 /label=conserved RBS 3341..3352 /label=BBa_B0034 CDS 3359..4081 /label=BBa_E0020 stem_loop 4101..4144 /label=stem_loop misc_feature 4090..4169 /label=BBa_B0010 terminator 4090..4169 /label=BBa_B0010 stem_loop 4185..4204 /label=T7 TE misc_feature 4211..4211 /label=stop polyA_site 4205..4218 /label=polya misc_feature 4178..4218 /label=BBa_B0012 terminator 4178..4218 /label=BBa_B0012 ORIGIN 1 agatctatga atgatgaaaa ctacagcacg acaatttaca accgtgttca gaccgagcgc 61 gtatacgaag attctgaccc ggccgaaaac ggcggcccgc tgtacgacga agtgcacgaa 121 gacgtacgcc gcgaagataa cctgtatgta aatgaactgg aaaaccaaga atacgacagc 181 gtggcagtct atcctgtggg tcgtcagggc cgtactagtg cgagtctgca gccagaaact 241 ggtgaatacg tcctgccgga cgaaccgtac agcaaagctc aggacccgca cccaggcgaa 301 cctacggcag atgaagacat ttctctggag gaactgctga gcccgaccaa ggaccatcag 361 agcgacagtg aagagcctca ggcgagcgac ccggaagaac cacaagccag cgacccggaa 421 gagccgcaag gtccagatcc ggaagaaccg caagaaaacg ggaacgaaat ggaggcggat 481 ctgccgagtc cgtctagttt caccattcaa aatagccgtg catttagcac gcgtgaaatt 541 tctccaacca gctatagtgc agatgacgta agtgagggca atgaatctgc ctctgcaagc 601 ccggagatca acctgtttgt taaagcgggc atcgatggtg aatctatcgg caattgtccg 661 tttagtcagc gtctgttcat gatcctgtgg ctgaaaggcg ttgttttcaa tgtgacgacg 721 gtggacctga aacgtaagcc agccgatctg cacaatctgg cgcctggtac ccatcctccg 781 tttctgactt ttaacggtga tgtgaagacc gatgtgaaca aaatcgaaga atttctggaa 841 gaaacgctga caccggaaaa gtatccacgt ctggccgcga agcatcgtga gtctaacaca 901 gcgggcatcg acatctttgt taaattcagc gcgtatatta agaatacaaa acagcagtct 961 aatgcagcac tggaacgcgg gctgaccaaa gcactgaaga aactggatga ttatctgaac 1021 actccactgc cagaggagat tgatgctgat actcgtggtg atgacgagaa agggagccgt 1081 cgcaaatttc tggacggtga tgagctgaca ctggcggatt gcaatctgct gcctaaactg 1141 catgtggtca aaattgttgc gaagaaatat cgcaattatg atttccctgc tgagatgacc 1201 ggtctgtggc gctatctgaa gaatgcttat gctcgcgatg agttcaccaa cacctgcgct 1261 gccgatagcg agattgagct ggcctatgcc gatgtcgcca aacgcctgtc tcgcagttaa 1321 ggatccatag aattactaga gtatggatcc ttacaggcgc gtggcattat gatgggctgg 1381 cagggccagg cgtggactcg gcgttgccgg tggcagttct tccagaaaca cgcgtggcgg 1441 cagctgcgga ctgcgcggct ccgggcgtgc ataacacagt gtggcgcggg tgatcagacc 1501 atccagcggc tgcagactat gcatccagcg cggcagaaaa tcccacgttt gcagccattt 1561 cggcaggtga cccggactgc gactttgcag aacattaacc agcaccacaa acgccagcag 1621 ggcgccaaaa ggcgtgccta cacccaccat ggcctgccag cctgccatac tgataccgaa 1681 aaccagagac ggcagcagca gaaaacagac cagcaggtac aggaccgcaa accaacgata 1741 tttcgcggtg cgtttgccca gcgctttggc catacggatc ggcagacgtg tacatggcag 1801 cggataccac agcaggatac cagagatatt aaagaagaag tgacacagag caatctggaa 1861 agaactactc agcttttcac gaggagacgc cagcgcagcc aggatagctg tcgtggtcgt 1921 accgatgtta gagcccagtg tcagcgggta cgcgcgttcg atagagataa cgcccaggcc 1981 aatcagagga gtgatagcag aagtaaagac agaagaactc tgtacgacaa aagtcatgct 2041 cgcacccacg accatcgcaa aatagcctgt cacccaggtg aaaggcgctg ggaagtcggt 2101 attaatcact ttctgaatga cgttcgccac ctgccctttc agcaggctat tcagcatttt 2161 caccagcaga atcagacagg tgcacagaac caccagacta cccgccagca ggatcagacc 2221 aactgccaga tccggcaggc cagtatccac gaaaatatgg ttgcatttct ccatggttgt 2281 gttagccagg ctaccaatag cctccacgcg agacatactt gtgctggctt ccttggtttc 2341 cggttggcac caaatacgaa tcaggctgtg gttacgcagg ctttcatcac cgacggcgat 2401 gctggtaatc acgcttttgt ccagttggat aatcagtttg gtgaacggtt ccgtgattac 2461 tttcagcagg tctggtgcgt cacggccgcc acgaatgtta aaactggcaa ccaccagacc 2521 ggtaacatga tgcaggtagc cggttgctgc ctccagcggc agcaggacca gaacgctcag 2581 ccagttgaaa cagtcatgaa cggtcgcacc agcaaaggca cggcgaaaat cggtacgatc 2641 gcctgcctgc atcagagcaa cgatcgtatt ggttacacta gtgccaatgt tgctgcccat 2701 aatgatagga atcgcgctgc ttacttccag cagaccagag cttaccatgc ttacaataat 2761 gcttgtagag gtgctagaac tctggaccag cacagtcacc agaatgccca caaccaggcc 2821 cgccaccgga ttagacagaa ttgcattatc cttgaaaata tcaccggcca ctttaccgcc 2881 agccagttga aacgcagagc tcagaacgtc cagagagcac acgaacaggt acagaaagcc 2941 cagcatcagc ggcactttca gcagcttagt cccaacttgt gccagtttct gactcagacg 3001 tggttcaggc ttttgctctt cttcctgcgc cagttttgca ggcagcggat cgtggcactc 3061 cagaacctcg ccatacgggc aagagtgctc cgtcagcgcg accggactca ggctgctaat 3121 agcgtacgtc gtagtacccg gaatgcgatg cagtacctga gggcttggga catacgcgaa 3181 ggctgcgcca tgaaccatgt gacgaccacg gactggcagc gggcttactg caggaccacc 3241 cagacgttcg ctatagctca tcatagatct tactagagtc cctatcagtg atagagattg 3301 acatccctat cagtgataga gatactgagc actactagag aaagaggaga aatactagat 3361 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg 3421 cgacgtgaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg 3481 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct 3541 cgtgaccacc ctgacctggg gcgtgcagtg cttcagccgc taccccgacc acatgaagca 3601 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt 3661 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt 3721 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa 3781 gctggagtac aactacatca gccacaacgt ctatatcacc gccgacaagc agaagaacgg 3841 catcaaggcc aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga 3901 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta 3961 cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct 4021 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaata 4081 atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt 4141 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg 4201 ggcctttctg cgtttata //