BBa_K287006
type: ComponentDefinition
persistent identity: BBa K287006
display id: BBa_K287006
version: 1
was generated by: iGEM to SBOL conversion
description: Double Channel with Cyanine reporter
created: 2009-10-20T11:00:00Z
modified: 2015-05-08T01:11:48Z
mutable provenance: This part comes from a Genomic Sequence from the NCBI.
owned by: james
owned by: myers
mutable description: This part is a composite from the Chloride-Sodium Channel with Cyanine reporter.
discontinued: false
dominant: false
group u list: _390_
m user id: 0
owner id: 4706
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: None
star: false
creator: Carlos Andres Sanchez
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K287003
component: BBa_B0012
component: BBa_B0034
component: BBa_R0040
component: BBa_B0010
component: BBa_E0020
component: BBa_K287004
sequence annotation: BBa_K287003
sequence annotation: BBa_K287004
sequence annotation: BBa_R0040
sequence annotation: BBa_B0034
sequence annotation: BBa_E0020
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K287006_sequence/1

BBa_K287003
type: Component
persistent identity: component2062124
display id: component2062124
version: 1
top level: BBa_K287006
definition: BBa_K287003
access: public

BBa_B0012
type: Component
persistent identity: component2062136
display id: component2062136
version: 1
top level: BBa_K287006
definition: BBa_B0012
access: public

BBa_B0034
type: Component
persistent identity: component2062132
display id: component2062132
version: 1
top level: BBa_K287006
definition: BBa_B0034
access: public

BBa_R0040
type: Component
persistent identity: component2062126
display id: component2062126
version: 1
top level: BBa_K287006
definition: p(tetR)
access: public

BBa_B0010
type: Component
persistent identity: component2062134
display id: component2062134
version: 1
top level: BBa_K287006
definition: BBa_B0010
access: public

BBa_E0020
type: Component
persistent identity: component2062133
display id: component2062133
version: 1
top level: BBa_K287006
definition: ecfp
access: public

BBa_K287004
type: Component
persistent identity: component2062125
display id: component2062125
version: 1
top level: BBa_K287006
definition: BBa_K287004
access: public

BBa_K287003
type: SequenceAnnotation
persistent identity: annotation2062124
display id: annotation2062124
version: 1
top level: BBa_K287006
location: https://synbiohub.org/public/igem/BBa_K287006/annotation2062124/range2062124/1
component: BBa_K287003

https://synbiohub.org/public/igem/BBa_K287006/annotation2062124/range2062124/1
type: Range
persistent identity: range2062124
display id: range2062124
version: 1
top level: BBa_K287006
direction: direction/reverse
start: 1
end: 1333
orientation: inline

BBa_K287004
type: SequenceAnnotation
persistent identity: annotation2062125
display id: annotation2062125
version: 1
top level: BBa_K287006
location: https://synbiohub.org/public/igem/BBa_K287006/annotation2062125/range2062125/1
component: BBa_K287004

https://synbiohub.org/public/igem/BBa_K287006/annotation2062125/range2062125/1
type: Range
persistent identity: range2062125
display id: range2062125
version: 1
top level: BBa_K287006
direction: direction/reverse
start: 1342
end: 3270
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2062126
display id: annotation2062126
version: 1
top level: BBa_K287006
location: https://synbiohub.org/public/igem/BBa_K287006/annotation2062126/range2062126/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K287006/annotation2062126/range2062126/1
type: Range
persistent identity: range2062126
display id: range2062126
version: 1
top level: BBa_K287006
direction: direction/forward
start: 3279
end: 3332
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2062132
display id: annotation2062132
version: 1
top level: BBa_K287006
location: https://synbiohub.org/public/igem/BBa_K287006/annotation2062132/range2062132/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K287006/annotation2062132/range2062132/1
type: Range
persistent identity: range2062132
display id: range2062132
version: 1
top level: BBa_K287006
direction: direction/reverse
start: 3341
end: 3352
orientation: inline

BBa_E0020
type: SequenceAnnotation
persistent identity: annotation2062133
display id: annotation2062133
version: 1
top level: BBa_K287006
location: https://synbiohub.org/public/igem/BBa_K287006/annotation2062133/range2062133/1
component: BBa_E0020

https://synbiohub.org/public/igem/BBa_K287006/annotation2062133/range2062133/1
type: Range
persistent identity: range2062133
display id: range2062133
version: 1
top level: BBa_K287006
direction: direction/reverse
start: 3359
end: 4081
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2062134
display id: annotation2062134
version: 1
top level: BBa_K287006
location: https://synbiohub.org/public/igem/BBa_K287006/annotation2062134/range2062134/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K287006/annotation2062134/range2062134/1
type: Range
persistent identity: range2062134
display id: range2062134
version: 1
top level: BBa_K287006
direction: direction/forward
start: 4090
end: 4169
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2062136
display id: annotation2062136
version: 1
top level: BBa_K287006
location: https://synbiohub.org/public/igem/BBa_K287006/annotation2062136/range2062136/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K287006/annotation2062136/range2062136/1
type: Range
persistent identity: range2062136
display id: range2062136
version: 1
top level: BBa_K287006
direction: direction/forward
start: 4178
end: 4218
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

ecfp
type: ComponentDefinition
persistent identity: BBa E0020
display id: BBa_E0020
version: 1
was generated by: iGEM to SBOL conversion
description: engineered cyan fluorescent protein derived from A. victoria GFP
created: 2004-03-02T12:00:00Z
modified: 2015-08-31T04:07:25Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: -- No description --
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Caitlin Conboy and Jennifer Braff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_E0020_sequence/1

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

BBa_K287003
type: ComponentDefinition
persistent identity: BBa K287003
display id: BBa_K287003
version: 1
was generated by: iGEM to SBOL conversion
description: Chloride channel protein
created: 2009-10-20T11:00:00Z
modified: 2015-05-08T01:11:48Z
mutable provenance: This part comes from a Genomic Sequence from the NCBI.
owned by: james
owned by: myers
mutable description: The chloride intracellular channel (CLIC) gene family has been implicated in chloride ion transport within various subcellular compartments.
discontinued: false
dominant: false
group u list: _390_
m user id: 0
owner id: 4706
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The restriction sites were added by PCR with designed prymers. Restriction site at 5' Bgl II, and 3' XhoI
star: false
creator: Carlos Andres Sanchez
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K287003_sequence/1

BBa_K287004
type: ComponentDefinition
persistent identity: BBa K287004
display id: BBa_K287004
version: 1
was generated by: iGEM to SBOL conversion
description: Sodium Phosphate dependant channel
created: 2009-10-20T11:00:00Z
modified: 2015-05-08T01:11:48Z
mutable provenance: This part comes from a Genomic Sequence from the NCBI, syntethized by Stratagene
owned by: james
owned by: myers
mutable description: This part is a Sodium Phosphate dependant channel
discontinued: false
dominant: false
group u list: _390_
m user id: 0
owner id: 4706
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The restriction sites were added by PCR with designed prymers. Restriction site at 5' XhoI, and 3' XhoI
star: false
creator: Carlos Andres Sanchez
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K287004_sequence/1

https://synbiohub.org/public/igem/BBa_K287006_sequence/1
type: Sequence
persistent identity: BBa K287006 sequence
display id: BBa_K287006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agatctatgaatgatgaaaactacagcacgacaatttacaaccgtgttcagaccgagcgcgtatacgaagattctgacccggccgaaaacggcggcccgctgtacgacgaagtgcacgaagacgtacgccgcgaagataacctgtatgtaaatgaactggaaaaccaagaatacgacagcgtggcagtctatcctgtgggtcgtcagggccgtactagtgcgagtctgcagccagaaactggtgaatacgtcctgccggacgaaccgtacagcaaagctcaggacccgcacccaggcgaacctacggcagatgaagacatttctctggaggaactgctgagcccgaccaaggaccatcagagcgacagtgaagagcctcaggcgagcgacccggaagaaccacaagccagcgacccggaagagccgcaaggtccagatccggaagaaccgcaagaaaacgggaacgaaatggaggcggatctgccgagtccgtctagtttcaccattcaaaatagccgtgcatttagcacgcgtgaaatttctccaaccagctatagtgcagatgacgtaagtgagggcaatgaatctgcctctgcaagcccggagatcaacctgtttgttaaagcgggcatcgatggtgaatctatcggcaattgtccgtttagtcagcgtctgttcatgatcctgtggctgaaaggcgttgttttcaatgtgacgacggtggacctgaaacgtaagccagccgatctgcacaatctggcgcctggtacccatcctccgtttctgacttttaacggtgatgtgaagaccgatgtgaacaaaatcgaagaatttctggaagaaacgctgacaccggaaaagtatccacgtctggccgcgaagcatcgtgagtctaacacagcgggcatcgacatctttgttaaattcagcgcgtatattaagaatacaaaacagcagtctaatgcagcactggaacgcgggctgaccaaagcactgaagaaactggatgattatctgaacactccactgccagaggagattgatgctgatactcgtggtgatgacgagaaagggagccgtcgcaaatttctggacggtgatgagctgacactggcggattgcaatctgctgcctaaactgcatgtggtcaaaattgttgcgaagaaatatcgcaattatgatttccctgctgagatgaccggtctgtggcgctatctgaagaatgcttatgctcgcgatgagttcaccaacacctgcgctgccgatagcgagattgagctggcctatgccgatgtcgccaaacgcctgtctcgcagttaaggatccatagaattactagagtatggatccttacaggcgcgtggcattatgatgggctggcagggccaggcgtggactcggcgttgccggtggcagttcttccagaaacacgcgtggcggcagctgcggactgcgcggctccgggcgtgcataacacagtgtggcgcgggtgatcagaccatccagcggctgcagactatgcatccagcgcggcagaaaatcccacgtttgcagccatttcggcaggtgacccggactgcgactttgcagaacattaaccagcaccacaaacgccagcagggcgccaaaaggcgtgcctacacccaccatggcctgccagcctgccatactgataccgaaaaccagagacggcagcagcagaaaacagaccagcaggtacaggaccgcaaaccaacgatatttcgcggtgcgtttgcccagcgctttggccatacggatcggcagacgtgtacatggcagcggataccacagcaggataccagagatattaaagaagaagtgacacagagcaatctggaaagaactactcagcttttcacgaggagacgccagcgcagccaggatagctgtcgtggtcgtaccgatgttagagcccagtgtcagcgggtacgcgcgttcgatagagataacgcccaggccaatcagaggagtgatagcag…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0020_sequence/1
type: Sequence
persistent identity: BBa E0020 sequence
display id: BBa_E0020_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K287003_sequence/1
type: Sequence
persistent identity: BBa K287003 sequence
display id: BBa_K287003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agatctatgaatgatgaaaactacagcacgacaatttacaaccgtgttcagaccgagcgcgtatacgaagattctgacccggccgaaaacggcggcccgctgtacgacgaagtgcacgaagacgtacgccgcgaagataacctgtatgtaaatgaactggaaaaccaagaatacgacagcgtggcagtctatcctgtgggtcgtcagggccgtactagtgcgagtctgcagccagaaactggtgaatacgtcctgccggacgaaccgtacagcaaagctcaggacccgcacccaggcgaacctacggcagatgaagacatttctctggaggaactgctgagcccgaccaaggaccatcagagcgacagtgaagagcctcaggcgagcgacccggaagaaccacaagccagcgacccggaagagccgcaaggtccagatccggaagaaccgcaagaaaacgggaacgaaatggaggcggatctgccgagtccgtctagtttcaccattcaaaatagccgtgcatttagcacgcgtgaaatttctccaaccagctatagtgcagatgacgtaagtgagggcaatgaatctgcctctgcaagcccggagatcaacctgtttgttaaagcgggcatcgatggtgaatctatcggcaattgtccgtttagtcagcgtctgttcatgatcctgtggctgaaaggcgttgttttcaatgtgacgacggtggacctgaaacgtaagccagccgatctgcacaatctggcgcctggtacccatcctccgtttctgacttttaacggtgatgtgaagaccgatgtgaacaaaatcgaagaatttctggaagaaacgctgacaccggaaaagtatccacgtctggccgcgaagcatcgtgagtctaacacagcgggcatcgacatctttgttaaattcagcgcgtatattaagaatacaaaacagcagtctaatgcagcactggaacgcgggctgaccaaagcactgaagaaactggatgattatctgaacactccactgccagaggagattgatgctgatactcgtggtgatgacgagaaagggagccgtcgcaaatttctggacggtgatgagctgacactggcggattgcaatctgctgcctaaactgcatgtggtcaaaattgttgcgaagaaatatcgcaattatgatttccctgctgagatgaccggtctgtggcgctatctgaagaatgcttatgctcgcgatgagttcaccaacacctgcgctgccgatagcgagattgagctggcctatgccgatgtcgccaaacgcctgtctcgcagttaaggatccatagaat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K287004_sequence/1
type: Sequence
persistent identity: BBa K287004 sequence
display id: BBa_K287004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tatggatccttacaggcgcgtggcattatgatgggctggcagggccaggcgtggactcggcgttgccggtggcagttcttccagaaacacgcgtggcggcagctgcggactgcgcggctccgggcgtgcataacacagtgtggcgcgggtgatcagaccatccagcggctgcagactatgcatccagcgcggcagaaaatcccacgtttgcagccatttcggcaggtgacccggactgcgactttgcagaacattaaccagcaccacaaacgccagcagggcgccaaaaggcgtgcctacacccaccatggcctgccagcctgccatactgataccgaaaaccagagacggcagcagcagaaaacagaccagcaggtacaggaccgcaaaccaacgatatttcgcggtgcgtttgcccagcgctttggccatacggatcggcagacgtgtacatggcagcggataccacagcaggataccagagatattaaagaagaagtgacacagagcaatctggaaagaactactcagcttttcacgaggagacgccagcgcagccaggatagctgtcgtggtcgtaccgatgttagagcccagtgtcagcgggtacgcgcgttcgatagagataacgcccaggccaatcagaggagtgatagcagaagtaaagacagaagaactctgtacgacaaaagtcatgctcgcacccacgaccatcgcaaaatagcctgtcacccaggtgaaaggcgctgggaagtcggtattaatcactttctgaatgacgttcgccacctgccctttcagcaggctattcagcattttcaccagcagaatcagacaggtgcacagaaccaccagactacccgccagcaggatcagaccaactgccagatccggcaggccagtatccacgaaaatatggttgcatttctccatggttgtgttagccaggctaccaatagcctccacgcgagacatacttgtgctggcttccttggtttccggttggcaccaaatacgaatcaggctgtggttacgcaggctttcatcaccgacggcgatgctggtaatcacgcttttgtccagttggataatcagtttggtgaacggttccgtgattactttcagcaggtctggtgcgtcacggccgccacgaatgttaaaactggcaaccaccagaccggtaacatgatgcaggtagccggttgctgcctccagcggcagcaggaccagaacgctcagccagttgaaacagtcatgaacggtcgcaccagcaaaggcacggcgaaaatcggtacgatcgcctgcctgcatcagagcaacgatcgtattggttacactagtgccaatgttgctgcccataatgataggaatcgcgctgcttacttccagcagaccagagcttaccatgcttacaataatgcttgtagaggtgctagaactctggaccagcacagtcaccagaatgcccacaaccaggcccgccaccggattagacagaattgcattatccttgaaaatatcaccggccactttaccgccagccagttgaaacgcagagctcagaacgtccagagagcacacgaacaggtacagaaagcccagcatcagcggcactttcagcagcttagtcccaacttgtgccagtttctgactcagacgtggttcaggcttttgctcttcttcctgcgccagttttgcaggcagcggatcgtggcactccagaacctcgccatacgggcaagagtgctccgtcagcgcgaccggactcaggctgctaatagcgtacgtcgtagtacccggaatgcgatgcagtacctgagggcttgggacatacgcgaaggctgcgccatgaaccatgtgacgaccacggactggcagcgggcttactgcaggaccacccagacgttcgctatagctcatcatagatct
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z