LOCUS       BBa_K290001             2640 bp    DNA     linear       29-Nov-2024
DEFINITION  constitutive RhlR with bicistronic LuxI - GFP controlled by pRhl
ACCESSION   BBa_K290001
VERSION     BBa_K290001.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             62..784
                     /label=rhlR
     misc_feature    785..817
                     /label=LVA
     misc_feature    824..848
                     /label=Help:Barcodes
     CDS             62..823
                     /label=BBa_C0071
     stem_loop       868..911
                     /label=stem_loop
     misc_feature    857..936
                     /label=BBa_B0010
     terminator      857..936
                     /label=BBa_B0010
     stem_loop       952..971
                     /label=T7 TE
     misc_feature    978..978
                     /label=stop
     polyA_site      972..985
                     /label=polya
     misc_feature    945..985
                     /label=BBa_B0012
     terminator      945..985
                     /label=BBa_B0012
     misc_feature    44..985
                     /label=BBa_I0466
     misc_feature    994..1013
                     /label=RhlR
     promoter        1012..1017
                     /label=-35
     promoter        1035..1040
                     /label=-10
     misc_feature    1046..1046
                     /label=start
     promoter        994..1046
                     /label=BBa_R0071
     misc_feature    1059..1062
                     /label=conserved
     RBS             1055..1066
                     /label=BBa_B0034
     misc_feature    1652..1683
                     /label=LVA
     CDS             1073..1651
                     /label=luxI
     misc_feature    1073..1690
                     /label=BBa_C0061
     misc_feature    1691..1715
                     /label=Help:Barcodes
     CDS             1073..1690
                     /label=BBa_C0061
     misc_feature    1728..1731
                     /label=conserved
     RBS             1724..1735
                     /label=BBa_B0034
     misc_feature    1742..2503
                     /label=BBa_E0024
     stem_loop       2523..2566
                     /label=stem_loop
     misc_feature    2512..2591
                     /label=BBa_B0010
     terminator      2512..2591
                     /label=BBa_B0010
     stem_loop       2607..2626
                     /label=T7 TE
     misc_feature    2633..2633
                     /label=stop
     polyA_site      2627..2640
                     /label=polya
     misc_feature    2600..2640
                     /label=BBa_B0012
     terminator      2600..2640
                     /label=BBa_B0012
     misc_feature    1724..2640
                     /label=BBa_E0424
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta
       61 gatgaggaat gacggaggct ttttgctgtg gtgggacggt ttgcgtagcg agatgcagcc
      121 gatccacgac agccagggcg tgttcgccgt cctggaaaag gaagtgcggc gcctgggctt
      181 cgattactac gcctatggcg tgcgccacac gattcccttc acccggccga agaccgaggt
      241 ccatggcacc tatcccaagg cctggctgga gcgataccag atgcagaact acggggccgt
      301 ggatccggcg atcctcaacg gcctgcgctc ctcggaaatg gtggtctgga gcgacagcct
      361 gttcgaccag agccggatgc tctggaacga ggctcgcgat tggggcctct gtgtcggcgc
      421 gaccttgccg atccgcgcgc cgaacaattt gctcagcgtg ctttccgtgg cgcgcgacca
      481 gcagaacatc tccagcttcg agcgcgagga aatccgcctg cggctgcgtt gcatgatcga
      541 gttgctgacc cagaagctga ccgacctgga gcatccgatg ctgatgtcca acccggtctg
      601 cctgagccat cgcgaacgcg agatcctgca atggaccgcc gacggcaaga gttccgggga
      661 aatcgccatc atcctgagca tctccgagag cacggtgaac ttccaccaca agaacatcca
      721 gaagaagttc gacgcgccga acaagacgct ggctgccgcc tacgccgcgg cgctgggtct
      781 catcgctgca aacgacgaaa actacgcttt agtagcttaa taacactgat agtgctagtg
      841 tagatcacta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
      901 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct
      961 tcgggtgggc ctttctgcgt ttatatacta gagtcctgtg aaatctggca gttaccgtta
     1021 gctttcgaat tggctaaaaa gtgttctact agagaaagag gagaaatact agatgactat
     1081 aatgataaaa aaatcggatt ttttggcaat tccatcggag gagtataaag gtattctaag
     1141 tcttcgttat caagtgttta agcaaagact tgagtgggac ttagttgtag aaaataacct
     1201 tgaatcagat gagtatgata actcaaatgc agaatatatt tatgcttgtg atgatactga
     1261 aaatgtaagt ggatgctggc gtttattacc tacaacaggt gattatatgc tgaaaagtgt
     1321 ttttcctgaa ttgcttggtc aacagagtgc tcccaaagat cctaatatag tcgaattaag
     1381 tcgttttgct gtaggtaaaa atagctcaaa gataaataac tctgctagtg aaattacaat
     1441 gaaactattt gaagctatat ataaacacgc tgttagtcaa ggtattacag aatatgtaac
     1501 agtaacatca acagcaatag agcgattttt aaagcgtatt aaagttcctt gtcatcgtat
     1561 tggagacaaa gaaattcatg tattaggtga tactaaatcg gttgtattgt ctatgcctat
     1621 taatgaacag tttaaaaaag cagtcttaaa tgctgcaaac gacgaaaact acgctttagt
     1681 agcttaataa ctctgatagt gctagtgtag atctctacta gagaaagagg agaaatacta
     1741 gatggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     1801 cggcgacgtg aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
     1861 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     1921 cctcgtgacc accctgacct ggggcgtgca gtgcttcagc cgctaccccg accacatgaa
     1981 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     2041 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     2101 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     2161 caagctggag tacaactaca tcagccacaa cgtctatatc accgccgaca agcagaagaa
     2221 cggcatcaag gccaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
     2281 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     2341 ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
     2401 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagag
     2461 gcctgctgca aacgacgaaa actacgctgc tgctgtttaa taatactaga gccaggcatc
     2521 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
     2581 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
//