LOCUS       BBa_K290002             2756 bp    DNA     linear       22-Nov-2024
DEFINITION  constitutive LuxR, with bicistronic RhlI - EYFP  controlled by lux 
            pL
ACCESSION   BBa_K290002
VERSION     BBa_K290002.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    62..63
                     /label=prefix
     misc_feature    235..235
                     /label=T
     misc_feature    553..553
                     /label=A
     CDS             62..811
                     /label=luxR
     misc_feature    62..817
                     /label=BBa_C0062
     misc_feature    818..842
                     /label=Help:Barcodes
     CDS             62..817
                     /label=BBa_C0062
     stem_loop       862..905
                     /label=stem_loop
     misc_feature    851..930
                     /label=BBa_B0010
     terminator      851..930
                     /label=BBa_B0010
     stem_loop       946..965
                     /label=T7 TE
     misc_feature    972..972
                     /label=stop
     polyA_site      966..979
                     /label=polya
     misc_feature    939..979
                     /label=BBa_B0012
     terminator      939..979
                     /label=BBa_B0012
     misc_feature    44..979
                     /label=BBa_I0462
     misc_feature    988..1007
                     /label=LuxR/HSL
     promoter        1102..1109
                     /label=-10
     promoter        1076..1081
                     /label=-35
     misc_feature    1115..1115
                     /label=start
     misc_feature    1117..1136
                     /label=Putative LuxR/HSL
     misc_feature    988..1138
                     /label=BBa_R0063
     promoter        988..1138
                     /label=BBa_R0063
     misc_feature    1151..1154
                     /label=conserved
     RBS             1147..1158
                     /label=BBa_B0034
     CDS             1165..1767
                     /label=rhlL
     misc_feature    1768..1800
                     /label=LVA
     misc_feature    1807..1831
                     /label=Help:Barcodes
     CDS             1165..1831
                     /label=BBa_C0070
     misc_feature    1844..1847
                     /label=conserved
     RBS             1840..1851
                     /label=BBa_B0034
     misc_feature    2614..2619
                     /label=stop
     misc_feature    2575..2613
                     /label=AAV
     misc_feature    1858..2619
                     /label=BBa_E0034
     stem_loop       2639..2682
                     /label=stem_loop
     misc_feature    2628..2707
                     /label=BBa_B0010
     terminator      2628..2707
                     /label=BBa_B0010
     stem_loop       2723..2742
                     /label=T7 TE
     misc_feature    2749..2749
                     /label=stop
     polyA_site      2743..2756
                     /label=polya
     misc_feature    2716..2756
                     /label=BBa_B0012
     terminator      2716..2756
                     /label=BBa_B0012
     misc_feature    1840..2756
                     /label=BBa_E0434
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta
       61 gatgaaaaac ataaatgccg acgacacata cagaataatt aataaaatta aagcttgtag
      121 aagcaataat gatattaatc aatgcttatc tgatatgact aaaatggtac attgtgaata
      181 ttatttactc gcgatcattt atcctcattc tatggttaaa tctgatattt caatcctaga
      241 taattaccct aaaaaatgga ggcaatatta tgatgacgct aatttaataa aatatgatcc
      301 tatagtagat tattctaact ccaatcattc accaattaat tggaatatat ttgaaaacaa
      361 tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg aaaacatcag gtcttatcac
      421 tgggtttagt ttccctattc atacggctaa caatggcttc ggaatgctta gttttgcaca
      481 ttcagaaaaa gacaactata tagatagttt atttttacat gcgtgtatga acataccatt
      541 aattgttcct tctctagttg ataattatcg aaaaataaat atagcaaata ataaatcaaa
      601 caacgattta accaaaagag aaaaagaatg tttagcgtgg gcatgcgaag gaaaaagctc
      661 ttgggatatt tcaaaaatat taggttgcag tgagcgtact gtcactttcc atttaaccaa
      721 tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt atttctaaag caattttaac
      781 aggagcaatt gattgcccat actttaaaaa ttaataacac tgatagtgct agtgtagatc
      841 actactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
      901 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
      961 gggcctttct gcgtttatat actagagacc tgtacgatcc tacaggtgct tatgttaagt
     1021 aattgtattc ccagcgatac aatagtgtga caaaaatcca atttattaga atcaaatgtc
     1081 aatccattac cgttttaatg atatataaca cgcaaaactt gcgacaaaca ataggtaata
     1141 ctagagaaag aggagaaata ctagatgatc gaactgctgt ccgaatccct ggaaggtctg
     1201 tccgctgcta tgatcgctga actgggtcgt taccgtcacc aggttttcat cgaaaaactg
     1261 ggttgggacg ttgtttccac ctcccgtgtt cgtgaccagg agttcgacca gttcgaccac
     1321 ccgcagaccc gttacatcgt tgctatgtcc cgtcagggta tctgcggttg cgctcgtctg
     1381 ctgccgacca ccgacgctta cctgctgaaa gacgttttcg cttacctgtg ctccgaaacc
     1441 ccgccgtccg acccgtccgt ttgggaactg tcccgttacg ctgcttccgc tgctgacgac
     1501 ccgcagctgg ctatgaaaat cttctggtcc tccctccagt gcgcttggta cctgggtgct
     1561 tcctccgttg ttgctgttac caccaccgct atggaacgtt acttcgttcg taacggtgtt
     1621 atcctccagc gtctgggtcc gccgcagaaa gttaaaggtg aaaccctggt tgctatctcc
     1681 ttcccggctt accaggaacg tggtctggaa atgctgctgc gttaccaccc ggaatggctc
     1741 cagggtgttc cgctgtccat ggctgttgct gcaaacgacg aaaactacgc tttagtagct
     1801 taataactct gatagtgcta gtgtagatct ctactagaga aagaggagaa atactagatg
     1861 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
     1921 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
     1981 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
     2041 gtgaccacct tcggctacgg cctgcaatgc ttcgcccgct accccgacca catgaagctg
     2101 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
     2161 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     2221 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     2281 ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc
     2341 atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
     2401 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     2461 ctgagctacc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     2521 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagaggcct
     2581 gctgcaaacg acgaaaacta cgctgctgct gtttaataat actagagcca ggcatcaaat
     2641 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
     2701 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata
//