LOCUS       BBa_K292004             1173 bp    DNA     linear       28-Nov-2024
DEFINITION  Double terminator + Kanamycin resistance cassette + Double ter
ACCESSION   BBa_K292004
VERSION     BBa_K292004.1  
FEATURES             Location/Qualifiers
     stem_loop       8..27
                     /label=T7 TE
     misc_feature    34..34
                     /label=stop
     polyA_site      28..41
                     /label=polya
     misc_feature    1..41
                     /label=BBa_B0012
     terminator      1..41
                     /label=BBa_B0012
     stem_loop       62..84
                     /label=stem_loop
     misc_feature    50..95
                     /label=BBa_B0011
     terminator      50..95
                     /label=BBa_B0011
     CDS             252..1070
                     /label=kanamycin resistance
     misc_feature    104..1070
                     /label=BBa_P1003
     stem_loop       1086..1105
                     /label=T7 TE
     misc_feature    1112..1112
                     /label=stop
     polyA_site      1106..1119
                     /label=polya
     misc_feature    1079..1119
                     /label=BBa_B0012
     terminator      1079..1119
                     /label=BBa_B0012
     stem_loop       1140..1162
                     /label=stem_loop
     misc_feature    1128..1173
                     /label=BBa_B0011
     terminator      1128..1173
                     /label=BBa_B0011
ORIGIN
        1 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagaga gagaatataa
       61 aaagccagat tattaatccg gcttttttat tattttacta gagctgatcc ttcaactcag
      121 caaaagttcg atttattcaa caaagccacg ttgtgtctca aaatctctga tgttacattg
      181 cacaagataa aaatatatca tcatgaacaa taaaactgtc tgcttacata aacagtaata
      241 caaggggtgt tatgagccat attcaacggg aaacgtcttg ctcccgtccg cgcttaaact
      301 ccaacatgga cgctgattta tatgggtata aatgggctcg cgataatgtc gggcaatcag
      361 gtgcgacaat ctatcgcttg tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg
      421 gcaaaggtag cgttgccaat gatgttacag atgagatggt ccgtctcaac tggctgacgg
      481 agtttatgcc tctcccgacc atcaagcatt ttatccgtac tcctgatgat gcgtggttac
      541 tcaccaccgc gattcctggg aaaacagcct tccaggtatt agaagaatat cctgattcag
      601 gtgaaaatat tgttgatgcg ctggccgtgt tcctgcgccg gttacattcg attcctgttt
      661 gtaattgtcc ttttaacagc gatcgtgtat ttcgtcttgc tcaggcgcaa tcacgcatga
      721 ataacggttt ggttgatgcg agtgattttg atgacgagcg taatggctgg cctgttgaac
      781 aagtctggaa agaaatgcac aagctcttgc cattctcacc ggattcagtc gtcactcatg
      841 gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt tgtattgatg
      901 ttggacgggt cggaatcgca gaccgttacc aggaccttgc cattctttgg aactgcctcg
      961 gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt gataatcctg
     1021 atatgaataa attgcagttt catttgatgc tcgatgagtt tttctaataa tactagagtc
     1081 acactggctc accttcgggt gggcctttct gcgtttatat actagagaga gaatataaaa
     1141 agccagatta ttaatccggc ttttttatta ttt
//