LOCUS       BBa_K292006             1277 bp    DNA     linear       04-May-2024
DEFINITION  Strong RBS + LacI repressor + double terminator
ACCESSION   BBa_K292006
VERSION     BBa_K292006.1  
FEATURES             Location/Qualifiers
     RBS             1..15
                     /label=RBS-1\Strong
     RBS             8..12
                     /label=RBS
     misc_feature    1..15
                     /label=BBa_B0030
     RBS             1..15
                     /label=BBa_B0030
     misc_feature    1111..1149
                     /label=LVA
     CDS             22..1149
                     /label=lacI-LVA
     misc_feature    22..1149
                     /label=BBa_C0012
     misc_feature    1150..1174
                     /label=Help:Barcodes
     CDS             22..1149
                     /label=BBa_C0012
     stem_loop       1190..1209
                     /label=T7 TE
     misc_feature    1216..1216
                     /label=stop
     polyA_site      1210..1223
                     /label=polya
     misc_feature    1183..1223
                     /label=BBa_B0012
     terminator      1183..1223
                     /label=BBa_B0012
     stem_loop       1244..1266
                     /label=stem_loop
     misc_feature    1232..1277
                     /label=BBa_B0011
     terminator      1232..1277
                     /label=BBa_B0011
     terminator      1183..1277
                     /label=BBa_B0014
ORIGIN
        1 attaaagagg agaaatacta gatggtgaat gtgaaaccag taacgttata cgatgtcgca
       61 gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt
      121 tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac
      181 cgcgtggcac aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt
      241 ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg
      301 ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg
      361 gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac
      421 caggatgcca ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc
      481 tctgaccaga cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc
      541 gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt
      601 tctgtctcgg cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt
      661 cagccgatag cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg
      721 caaatgctga atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg
      781 ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcggta
      841 gtgggatacg acgataccga agacagctca tgttatatcc cgccgttaac caccatcaaa
      901 caggattttc gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc
      961 caggcggtga agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg
     1021 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     1081 cgacaggttt cccgactgga aagcgggcag gctgcaaacg acgaaaacta cgctttagta
     1141 gcttaataac tctgatagtg ctagtgtaga tctctactag agtcacactg gctcaccttc
     1201 gggtgggcct ttctgcgttt atatactaga gagagaatat aaaaagccag attattaatc
     1261 cggctttttt attattt
//