BBa_B0013 1 BBa_B0013 TE from coliphage T7 (+/-) 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Strong transcriptional terminator consisting of a 20 bp stem-loop that has been engineered to be bidirectional. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Subsequently mutations were introduced to make the terminator bi-directional (i.e., AAAAAA insertion on 5' side of the stem loop). Additional mutations were introduced on the 3' side of the stem loop to increase the number of T's and eliminate any promoter -10 site that might be present to avoid initiation of transcription of whatever is downstream.<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation7021 1 BBa_B0013 range7021 1 1 47 annotation1696 1 C range1696 1 10 10 annotation1691 1 T7 TE range1691 1 14 33 annotation1695 1 C range1695 1 37 37 annotation1698 1 polya range1698 1 34 47 annotation1692 1 stop range1692 1 40 40 annotation1697 1 '' range1697 1 1 6 BBa_K294001 1 BBa_K294001 Protein generator that produces E. coli heat shock protein hsp15 2009-06-26T11:00:00Z 2015-05-08T01:11:49Z This is a composite part of three basic parts. E. coli heat shock protein hsp15 protein generator. Designed for use in the bacterial lava lamp system but could be used wherever you need a heat shock protein. false false _397_ 0 135 84 Not in stock false This part was assembled by BioBrick assembly. We selected a strong RBS in designing this protein coding sequence. We selected a weak transcriptional terminator because we wanted some transcriptional read-through from the protein generator. false Meagan Lizarazo component2006755 1 BBa_B0034 component2006760 1 BBa_B0013 component2006758 1 BBa_K294000 annotation2006755 1 BBa_B0034 range2006755 1 1 12 annotation2006758 1 BBa_K294000 range2006758 1 19 420 annotation2006760 1 BBa_B0013 range2006760 1 429 475 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K294000 1 BBa_K294000 This is the coding sequence for the heat shock protein hsp15 from E. coli 2009-06-26T11:00:00Z 2015-05-08T01:11:49Z hsp15 is derived from the genome of E. coli K12. We retrieved the [http://BioCyc.org/ECOLI/NEW-IMAGE?type=GENE&object=G7743 sequence] from the Ecocyc database on June 26th 2009. This coding sequence produces a protein that is involved in the heat shock response of E. coli. We are using this part to regulate the activity of a heat shock promoter as part of a bacterial lava lamp. false false _397_ 0 135 84 Not in stock true We chose this heat shock protein because it operates well in multiple bacteria including E. coli and B. subtilis and our project involves both organisms. false Bartholomew Canton annotation2006746 1 hslR range2006746 1 1 402 annotation2006750 1 start codon range2006750 1 1 3 BBa_K294000_sequence 1 atgaaagagaaacctgctgttgaggttcgactggataaatggctatgggctgcccgtttttataaaacccgcgcgctggcccgtgaaatgattgaaggcggtaaggtgcattacaacgggcagcgcagcaagccgagcaaaatcgtcgagctgaatgccacgctcactctgcgccagggaaatgacgaacgcacggtgattgtaaaggcgattactgaacagcgtcgccccgccagcgaggcagccttgctgtatgaagagactgcggaaagtgtagagaaacgcgaaaaaatggcgctggcacgtaaacttaatgccttaaccatgccgcacccggaccgacgcccggacaaaaaagagcgccgcgacctgttacgatttaaacacggcgacagtgaataa BBa_B0034_sequence 1 aaagaggagaaa BBa_B0013_sequence 1 aaaaaatcaaactggctcaccttcgggtgggcctttttgcgtttata BBa_K294001_sequence 1 aaagaggagaaatactagatgaaagagaaacctgctgttgaggttcgactggataaatggctatgggctgcccgtttttataaaacccgcgcgctggcccgtgaaatgattgaaggcggtaaggtgcattacaacgggcagcgcagcaagccgagcaaaatcgtcgagctgaatgccacgctcactctgcgccagggaaatgacgaacgcacggtgattgtaaaggcgattactgaacagcgtcgccccgccagcgaggcagccttgctgtatgaagagactgcggaaagtgtagagaaacgcgaaaaaatggcgctggcacgtaaacttaatgccttaaccatgccgcacccggaccgacgcccggacaaaaaagagcgccgcgacctgttacgatttaaacacggcgacagtgaataatactagagaaaaaatcaaactggctcaccttcgggtgggcctttttgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z