##gff-version 3
##sequence-region BBa_K294112 1 1641
BBa_K294112	.	CDS	1	706	.	+	0	ID=BBa_E1010;Name=BBa_E1010
BBa_K294112	.	engineered_tag	682	706	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K294112	.	CDS	1	675	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K294112	.	engineered_region	715	1641	.	+	0	ID=BBa_J54103;Name=BBa_J54103
BBa_K294112	.	tag	729	734	.	+	0	ID=annotation2152491;Name=BamHI;Parent=BBa_J54103
BBa_K294112	.	engineered_region	1514	1593	.	+	0	ID=annotation2152499;Name=BBa_B0010;Parent=BBa_J54103
BBa_K294112	.	tag	741	746	.	+	0	ID=annotation2152493;Name=BglII;Parent=BBa_J54103
BBa_K294112	.	engineered_region	715	734	.	+	0	ID=annotation2152489;Name=BBa_J54033;Parent=BBa_J54103
BBa_K294112	.	engineered_region	763	778	.	+	0	ID=annotation2152496;Name=BBa_B0030;Parent=BBa_J54103
BBa_K294112	.	tag	755	760	.	+	0	ID=annotation2152494;Name=MulI;Parent=BBa_J54103
BBa_K294112	.	engineered_region	1601	1641	.	+	0	ID=annotation2152500;Name=BBa_B0012;Parent=BBa_J54103
BBa_K294112	.	engineered_region	741	760	.	+	0	ID=annotation2152492;Name=BBa_J54025;Parent=BBa_J54103
BBa_K294112	.	ribosome_entry_site	763	778	.	+	0	ID=annotation2152495;Name=RBS-1\Strong;Parent=BBa_J54103
BBa_K294112	.	stem_loop	1613	1632	.	+	0	ID=annotation2152502;Name=T7 TE;Parent=BBa_J54103
BBa_K294112	.	ribosome_entry_site	770	776	.	+	0	ID=annotation2152497;Name=RBS;Parent=BBa_J54103
BBa_K294112	.	engineered_region	785	1504	.	+	0	ID=annotation2152498;Name=BBa_E0040;Parent=BBa_J54103
BBa_K294112	.	stem_loop	1525	1568	.	+	0	ID=annotation2152501;Name=stem_loop;Parent=BBa_J54103
BBa_K294112	.	tag	715	720	.	+	0	ID=annotation2152490;Name=SalI;Parent=BBa_J54103
>BBa_K294112
atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagt
tcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtcc
gctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatc
ccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgtta
ccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccga
cggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaa
ggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaa
aaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgt
tgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctacta
gaggtcgactgacgactggatcctgtcggagatctgtcggataacgcgtgagattaaagaggagaaatactagatgcgt
aaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctg
tcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgt
tccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcat
gactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaaga
cacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatgg
aaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatgga
atcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactc
caattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacga
aaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataa
taatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcg
gtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata