BBa_B0030 1 BBa_B0030 RBS.1 (strong) -- modified from R. Weiss 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _44_46_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;orig&quot; in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation1701 1 RBS-1\Strong range1701 1 1 15 annotation1702 1 RBS range1702 1 8 12 annotation7025 1 BBa_B0030 range7025 1 1 15 BBa_I14032 1 P(Lac) IQ promoter P(Lac) IQ 2004-08-03T11:00:00Z 2015-08-31T04:07:37Z Plasmid pMAL-p2X Released HQ 2013 Constitutive Promoter, High Transcription false true _4_ 0 171 7 In stock false true Vikram Vijayan, Allen Hsu, Lawrence Fomundam annotation1028344 1 -10 range1028344 1 26 31 annotation1028342 1 P(Lac) IQ range1028342 1 1 37 annotation1028343 1 -35 range1028343 1 3 8 BBa_K294354 1 BBa_K294354 test 2010-10-01T11:00:00Z 2015-05-08T01:11:49Z tggtgcaaaacctttcgcggtatggcatgatagcgcctactagagattaaagaggagaaatactagatggcgtggctggtcggcaagccgtcgctcgaac gatcatggaatgcgatactaagttttccggaatctggattccaactggaatgcaggaatacgatcggtagtagcgtttttagctcgcattttacgctgca ttttcgagtggcacgtcgcttgcttcatttctcgtgccgccgattcaccgaaactcagaaagagccaacgcaagctctatggtggtgcgaattgcccact gctcccgcgcccagacgccgcggaaccggcctaaaagcggcattaatcttggcgaaggacaattcaaatccaagggagagtaagatgagcatcacacgca gacatgtcatcgttcagggtggcgtcattgcagcaggcctgctcgccagcggcctaccggggacaaaagccttcgcgcagataccgtcaatcccttggcg gcgctcactccagggcttggcctggaacgacccgatcatcgagacctatcgcgacgcagtgcgccttctcaacgcccttcccgccagcgacaaattcaac tgggtcaacctctcgaaaattcacggcagcggtgacgtcgtcaaatactgcccgcatgggaactggtatttcctgccgtggcacagggcctataccgcta tgtacgagcgcatcgttcggcacgtgaccaagaacaacgatttcgctatgccgttctgggactggaccgacaatccgtacctgcccgaagtgttcacaat gcaaaagacgcccgacggcaaggacaatccactttatgtttcgtcgcgcacctggccaatcacgcagccgatgccggacaatatagttgggccacaggtt ctcaacaccatcctaacggcgaagccatacgaggtcttcggcaccacccgccccgagggacagaactcactcgatccttcctgggtcaccaccagcagcg gcacgcagggggcgctggaatacacaccgcacaatcaggtgcacaacaatatcggtggctggatgccggaaatgtcgtcgccccgcgatccgatcttctt catgcatcattgcaacatcgaccgcatctgggcgacctggaatttgcgcaacgccaacagcacggatcgactctgggccgacatgccgttcaccgacaat ttctacgatgtagacggcaacttctggtccccgaaggtctctgacctttatgttccagaggaactcggatacaattatggtttccggacctacttcaagg tcgcggcggcgagcgccaaaacgctggccctgaacgataaactcacgtccgtgatcgcggcgacggcgaccgatgctgcaatcgccggcgtgacgaccac ctccacggacaacagcaaggcggcaacggaaaacgtgccgctttcgctgccgatcaagatcccggcgggcgcattgcaggagatcgtccgccaaccgcct ctgccatccggcatggatacaatggatttcggcgcggcacaggagcaggcggcctccgctcctcgtgtgctggcattcctgcgcgatgtcgagatcacca gtgccagcacgaccagcgttcgggtgtttctcggcaagaacgaccttaaggccgatacgcccgtcaccgatccccattacgtcggttctttcgccgttct cggtcatgacggcgaccatcatcgcaaaccatccttcgtcctcgatctgacggacgcgatccagcgggtttacggcggaagggggcagacggatggcgag gccatcgacctccagctcattcctgtcggatcgggagcgggcaaacccggcgccgtagagcccgcaaagctagaaatagccatagtgtccgcctaa test test false false _397_ 0 5245 9 It's complicated false design note false Kazuaki Amikura component2081165 1 BBa_K193600 component2081163 1 BBa_B0030 component2081161 1 BBa_I14032 annotation2081165 1 BBa_K193600 range2081165 1 67 1896 annotation2081163 1 BBa_B0030 range2081163 1 46 60 annotation2081161 1 BBa_I14032 range2081161 1 1 37 BBa_K193600 1 BBa_K193600 melA gene 2009-10-13T11:00:00Z 2015-05-08T01:11:16Z The gene sequence was obtained from GENEART. melA is coding sequence of tyrosinase. Tyrosinase is enzyme which converts from tyrosine to dopaquinone. Dopaquinone is intermediate product of melanin biosynthesis pathway. false false _288_ 0 5245 9 Not in stock false We added restriction enzyme sites (Prefix-Suffix) to the upstream and down stream of the ORF of melA. false Kazuaki Amikura BBa_I14032_sequence 1 tggtgcaaaacctttcgcggtatggcatgatagcgcc BBa_B0030_sequence 1 attaaagaggagaaa BBa_K294354_sequence 1 tggtgcaaaacctttcgcggtatggcatgatagcgcctactagagattaaagaggagaaatactagatggcgtggctggtcggcaagccgtcgctcgaacgatcatggaatgcgatactaagttttccggaatctggattccaactggaatgcaggaatacgatcggtagtagcgtttttagctcgcattttacgctgcattttcgagtggcacgtcgcttgcttcatttctcgtgccgccgattcaccgaaactcagaaagagccaacgcaagctctatggtggtgcgaattgcccactgctcccgcgcccagacgccgcggaaccggcctaaaagcggcattaatcttggcgaaggacaattcaaatccaagggagagtaagatgagcatcacacgcagacatgtcatcgttcagggtggcgtcattgcagcaggcctgctcgccagcggcctaccggggacaaaagccttcgcgcagataccgtcaatcccttggcggcgctcactccagggcttggcctggaacgacccgatcatcgagacctatcgcgacgcagtgcgccttctcaacgcccttcccgccagcgacaaattcaactgggtcaacctctcgaaaattcacggcagcggtgacgtcgtcaaatactgcccgcatgggaactggtatttcctgccgtggcacagggcctataccgctatgtacgagcgcatcgttcggcacgtgaccaagaacaacgatttcgctatgccgttctgggactggaccgacaatccgtacctgcccgaagtgttcacaatgcaaaagacgcccgacggcaaggacaatccactttatgtttcgtcgcgcacctggccaatcacgcagccgatgccggacaatatagttgggccacaggttctcaacaccatcctaacggcgaagccatacgaggtcttcggcaccacccgccccgagggacagaactcactcgatccttcctgggtcaccaccagcagcggcacgcagggggcgctggaatacacaccgcacaatcaggtgcacaacaatatcggtggctggatgccggaaatgtcgtcgccccgcgatccgatcttcttcatgcatcattgcaacatcgaccgcatctgggcgacctggaatttgcgcaacgccaacagcacggatcgactctgggccgacatgccgttcaccgacaatttctacgatgtagacggcaacttctggtccccgaaggtctctgacctttatgttccagaggaactcggatacaattatggtttccggacctacttcaaggtcgcggcggcgagcgccaaaacgctggccctgaacgataaactcacgtccgtgatcgcggcgacggcgaccgatgctgcaatcgccggcgtgacgaccacctccacggacaacagcaaggcggcaacggaaaacgtgccgctttcgctgccgatcaagatcccggcgggcgcattgcaggagatcgtccgccaaccgcctctgccatccggcatggatacaatggatttcggcgcggcacaggagcaggcggcctccgctcctcgtgtgctggcattcctgcgcgatgtcgagatcaccagtgccagcacgaccagcgttcgggtgtttctcggcaagaacgaccttaaggccgatacgcccgtcaccgatccccattacgtcggttctttcgccgttctcggtcatgacggcgaccatcatcgcaaaccatccttcgtcctcgatctgacggacgcgatccagcgggtttacggcggaagggggcagacggatggcgaggccatcgacctccagctcattcctgtcggatcgggagcgggcaaacccggcgccgtagagcccgcaaagctagaaatagccatagtgtccgcctaa BBa_K193600_sequence 1 atggcgtggctggtcggcaagccgtcgctcgaacgatcatggaatgcgatactaagttttccggaatctggattccaactggaatgcaggaatacgatcggtagtagcgtttttagctcgcattttacgctgcattttcgagtggcacgtcgcttgcttcatttctcgtgccgccgattcaccgaaactcagaaagagccaacgcaagctctatggtggtgcgaattgcccactgctcccgcgcccagacgccgcggaaccggcctaaaagcggcattaatcttggcgaaggacaattcaaatccaagggagagtaagatgagcatcacacgcagacatgtcatcgttcagggtggcgtcattgcagcaggcctgctcgccagcggcctaccggggacaaaagccttcgcgcagataccgtcaatcccttggcggcgctcactccagggcttggcctggaacgacccgatcatcgagacctatcgcgacgcagtgcgccttctcaacgcccttcccgccagcgacaaattcaactgggtcaacctctcgaaaattcacggcagcggtgacgtcgtcaaatactgcccgcatgggaactggtatttcctgccgtggcacagggcctataccgctatgtacgagcgcatcgttcggcacgtgaccaagaacaacgatttcgctatgccgttctgggactggaccgacaatccgtacctgcccgaagtgttcacaatgcaaaagacgcccgacggcaaggacaatccactttatgtttcgtcgcgcacctggccaatcacgcagccgatgccggacaatatagttgggccacaggttctcaacaccatcctaacggcgaagccatacgaggtcttcggcaccacccgccccgagggacagaactcactcgatccttcctgggtcaccaccagcagcggcacgcagggggcgctggaatacacaccgcacaatcaggtgcacaacaatatcggtggctggatgccggaaatgtcgtcgccccgcgatccgatcttcttcatgcatcattgcaacatcgaccgcatctgggcgacctggaatttgcgcaacgccaacagcacggatcgactctgggccgacatgccgttcaccgacaatttctacgatgtagacggcaacttctggtccccgaaggtctctgacctttatgttccagaggaactcggatacaattatggtttccggacctacttcaaggtcgcggcggcgagcgccaaaacgctggccctgaacgataaactcacgtccgtgatcgcggcgacggcgaccgatgctgcaatcgccggcgtgacgaccacctccacggacaacagcaaggcggcaacggaaaacgtgccgctttcgctgccgatcaagatcccggcgggcgcattgcaggagatcgtccgccaaccgcctctgccatccggcatggatacaatggatttcggcgcggcacaggagcaggcggcctccgctcctcgtgtgctggcattcctgcgcgatgtcgagatcaccagtgccagcacgaccagcgttcgggtgtttctcggcaagaacgaccttaaggccgatacgcccgtcaccgatccccattacgtcggttctttcgccgttctcggtcatgacggcgaccatcatcgcaaaccatccttcgtcctcgatctgacggacgcgatccagcgggtttacggcggaagggggcagacggatggcgaggccatcgacctccagctcattcctgtcggatcgggagcgggcaaacccggcgccgtagagcccgcaaagctagaaatagccatagtgtccgcctaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z